Hubei tombus-like virus 9

Taxonomy: Viruses; Riboviria; unclassified Riboviria; unclassified RNA viruses ShiM-2016

Average proteome isoelectric point is 7.5

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1L3KGI2|A0A1L3KGI2_9VIRU RNA-directed RNA polymerase OS=Hubei tombus-like virus 9 OX=1923296 PE=4 SV=1
MM1 pKa = 7.55VSVQGYY7 pKa = 8.33NSQPKK12 pKa = 8.45FHH14 pKa = 7.33PPGVRR19 pKa = 11.84ISDD22 pKa = 3.74HH23 pKa = 5.79ATSSGRR29 pKa = 11.84EE30 pKa = 4.09KK31 pKa = 10.71NRR33 pKa = 11.84HH34 pKa = 4.47VLVDD38 pKa = 3.24TGVPGVGLCYY48 pKa = 9.62THH50 pKa = 6.93NNSADD55 pKa = 3.39NLLRR59 pKa = 11.84GLGEE63 pKa = 4.2RR64 pKa = 11.84LLMVPTGDD72 pKa = 3.81TFSQPPRR79 pKa = 11.84PCADD83 pKa = 2.89AFRR86 pKa = 11.84CLEE89 pKa = 3.81EE90 pKa = 3.88YY91 pKa = 10.05RR92 pKa = 11.84RR93 pKa = 11.84KK94 pKa = 10.08VVSKK98 pKa = 10.34CPKK101 pKa = 7.71QTGPLEE107 pKa = 4.12RR108 pKa = 11.84DD109 pKa = 3.58EE110 pKa = 5.44FVSLYY115 pKa = 10.66DD116 pKa = 3.29GPKK119 pKa = 8.65RR120 pKa = 11.84KK121 pKa = 9.55RR122 pKa = 11.84YY123 pKa = 8.27EE124 pKa = 3.62AAARR128 pKa = 11.84NLADD132 pKa = 5.92RR133 pKa = 11.84EE134 pKa = 4.22LQKK137 pKa = 11.11ADD139 pKa = 2.62WDD141 pKa = 3.75INVFIKK147 pKa = 10.87DD148 pKa = 3.46EE149 pKa = 4.66TVCSWSKK156 pKa = 10.83VDD158 pKa = 3.7PAPRR162 pKa = 11.84LISPRR167 pKa = 11.84SPEE170 pKa = 3.75YY171 pKa = 10.49CLEE174 pKa = 4.37LGCYY178 pKa = 9.11IKK180 pKa = 10.57PVEE183 pKa = 4.12KK184 pKa = 10.52LLYY187 pKa = 9.96KK188 pKa = 10.56AVARR192 pKa = 11.84VWGEE196 pKa = 3.48VTIAKK201 pKa = 9.76GLNFNQRR208 pKa = 11.84GEE210 pKa = 4.62LIQQKK215 pKa = 8.68WEE217 pKa = 4.18SFTEE221 pKa = 4.3PVAVGLDD228 pKa = 3.21ASRR231 pKa = 11.84FDD233 pKa = 3.5QHH235 pKa = 7.3VSTDD239 pKa = 3.34ALEE242 pKa = 4.53FEE244 pKa = 4.76HH245 pKa = 7.12GFYY248 pKa = 9.29TQLYY252 pKa = 8.09PRR254 pKa = 11.84SKK256 pKa = 10.6KK257 pKa = 10.79LPFLLSKK264 pKa = 10.72QLLNEE269 pKa = 3.41GRR271 pKa = 11.84AYY273 pKa = 11.15VDD275 pKa = 3.54DD276 pKa = 4.79KK277 pKa = 11.28KK278 pKa = 10.87IEE280 pKa = 3.9YY281 pKa = 9.55SVRR284 pKa = 11.84GSRR287 pKa = 11.84MSGDD291 pKa = 3.39MNTALGNCLIMTSLVWGYY309 pKa = 10.56LKK311 pKa = 10.46EE312 pKa = 4.11RR313 pKa = 11.84SVVGKK318 pKa = 9.97LINDD322 pKa = 3.67GDD324 pKa = 4.17DD325 pKa = 3.35CVVIMEE331 pKa = 4.64KK332 pKa = 10.77ADD334 pKa = 3.67LFRR337 pKa = 11.84FMDD340 pKa = 5.15GLTEE344 pKa = 4.19WFTQRR349 pKa = 11.84GFTMKK354 pKa = 10.24VEE356 pKa = 4.37SPVYY360 pKa = 8.5TLEE363 pKa = 4.36EE364 pKa = 3.93IEE366 pKa = 4.77FCQCHH371 pKa = 5.45PVFNGEE377 pKa = 4.36SYY379 pKa = 10.19TMCRR383 pKa = 11.84NVFKK387 pKa = 11.1ALFTDD392 pKa = 4.22VAHH395 pKa = 7.23VGRR398 pKa = 11.84TWRR401 pKa = 11.84EE402 pKa = 3.35VVGIRR407 pKa = 11.84EE408 pKa = 4.44SISVAGAVWAKK419 pKa = 9.86GIPVLSAFYY428 pKa = 10.52RR429 pKa = 11.84SLSTGAPQVVPRR441 pKa = 11.84HH442 pKa = 5.96SGTWWNAQGCNTGTVEE458 pKa = 3.66ITPASRR464 pKa = 11.84EE465 pKa = 4.05SFAKK469 pKa = 10.43AFHH472 pKa = 7.08LDD474 pKa = 3.4PAEE477 pKa = 3.94QAAIEE482 pKa = 4.11QMYY485 pKa = 8.4DD486 pKa = 3.42TLPQLPFSDD495 pKa = 4.37PNRR498 pKa = 11.84MLTYY502 pKa = 10.67NPTIPADD509 pKa = 3.49QYY511 pKa = 10.97PILISEE517 pKa = 4.75PLQVLLFNGPP527 pKa = 3.34

Molecular weight: 59.41 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1L3KGI2|A0A1L3KGI2_9VIRU RNA-directed RNA polymerase OS=Hubei tombus-like virus 9 OX=1923296 PE=4 SV=1
MM1 pKa = 7.4ARR3 pKa = 11.84KK4 pKa = 9.2KK5 pKa = 10.95QMTKK9 pKa = 9.65PRR11 pKa = 11.84ARR13 pKa = 11.84RR14 pKa = 11.84SRR16 pKa = 11.84KK17 pKa = 8.72QGKK20 pKa = 9.19KK21 pKa = 9.41SSAVPVPKK29 pKa = 10.49RR30 pKa = 11.84MLEE33 pKa = 4.03LAEE36 pKa = 4.94LLHH39 pKa = 6.35NPCDD43 pKa = 3.84ADD45 pKa = 3.48IPRR48 pKa = 11.84GIYY51 pKa = 9.36PGEE54 pKa = 3.96VGAVEE59 pKa = 4.1RR60 pKa = 11.84FIWEE64 pKa = 3.84QAFVGVNNTACFYY77 pKa = 11.01AFHH80 pKa = 7.67PNTGAIIYY88 pKa = 9.77SNQTTSSGALAMTTTASFAPGQTFLSANAAKK119 pKa = 10.44VRR121 pKa = 11.84GLAACIQVACSSLSVTAITGEE142 pKa = 3.67ISLGVLTADD151 pKa = 3.2VVTNNLLTTTTDD163 pKa = 2.34QWFNMLQARR172 pKa = 11.84GPISRR177 pKa = 11.84DD178 pKa = 2.73IKK180 pKa = 9.15EE181 pKa = 4.39VKK183 pKa = 9.02WYY185 pKa = 9.53PGLRR189 pKa = 11.84DD190 pKa = 3.78NSFTTYY196 pKa = 10.57NGSLNSLATLQATGSDD212 pKa = 3.38LSDD215 pKa = 3.13THH217 pKa = 6.27VVCIAIRR224 pKa = 11.84NVPVGTTINVRR235 pKa = 11.84TTYY238 pKa = 9.78VAEE241 pKa = 4.31WSPKK245 pKa = 7.81LTVGITPSTAVSPGTNHH262 pKa = 7.32LAAANILHH270 pKa = 6.62KK271 pKa = 10.82SKK273 pKa = 10.95PNWFHH278 pKa = 6.41NAGSGLGNAASGFATAFMQSVGQNAADD305 pKa = 3.41QTMKK309 pKa = 10.93YY310 pKa = 7.97ITSAGANAGNNMARR324 pKa = 11.84RR325 pKa = 11.84GTVYY329 pKa = 10.49AIEE332 pKa = 4.19EE333 pKa = 4.22LAEE336 pKa = 4.03EE337 pKa = 4.55LGPRR341 pKa = 11.84LLTLL345 pKa = 4.11

Molecular weight: 36.74 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2

0

2

872

345

527

436.0

48.07

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

8.945 ± 2.162

2.064 ± 0.349

4.243 ± 0.928

5.39 ± 1.085

4.014 ± 0.469

7.225 ± 0.341

1.835 ± 0.054

4.243 ± 0.389

4.702 ± 0.366

8.372 ± 0.31

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.179 ± 0.079

4.931 ± 0.985

5.734 ± 0.622

3.67 ± 0.109

5.849 ± 0.523

6.995 ± 0.307

7.225 ± 1.658

7.569 ± 0.512

1.72 ± 0.154

3.096 ± 0.441
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski