Rhizobium sp. Leaf306

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Alphaproteobacteria; Hyphomicrobiales; Rhizobiaceae; Rhizobium/Agrobacterium group; Rhizobium; unclassified Rhizobium

Average proteome isoelectric point is 6.33

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 4551 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A0Q5D4H8|A0A0Q5D4H8_9RHIZ Uncharacterized protein OS=Rhizobium sp. Leaf306 OX=1736330 GN=ASG19_20420 PE=4 SV=1
MM1 pKa = 7.46TKK3 pKa = 9.47TVLNALGEE11 pKa = 4.22TLYY14 pKa = 11.38YY15 pKa = 10.75SGTSKK20 pKa = 10.83AWISATGAGTALSGTAANDD39 pKa = 3.32SMYY42 pKa = 11.02GDD44 pKa = 3.71SAVNVTMSGGAGDD57 pKa = 4.08DD58 pKa = 3.44VYY60 pKa = 11.88YY61 pKa = 10.51LYY63 pKa = 11.33SSINRR68 pKa = 11.84AAEE71 pKa = 3.83AAGQGIDD78 pKa = 4.87TVDD81 pKa = 2.44TWMSYY86 pKa = 7.33TLPDD90 pKa = 3.94NIEE93 pKa = 4.04NLRR96 pKa = 11.84VTGDD100 pKa = 2.93NRR102 pKa = 11.84FAFGNDD108 pKa = 2.95LDD110 pKa = 4.95NIISGSSSRR119 pKa = 11.84QTFDD123 pKa = 2.94GGAGNDD129 pKa = 3.49VLTGGGGADD138 pKa = 3.32TFIVAKK144 pKa = 10.77GNGSDD149 pKa = 5.3LITDD153 pKa = 4.3FSADD157 pKa = 3.34DD158 pKa = 4.21KK159 pKa = 11.4IRR161 pKa = 11.84LDD163 pKa = 4.0GYY165 pKa = 10.68SFTSFEE171 pKa = 4.12QVGNSLTQEE180 pKa = 4.29GANLRR185 pKa = 11.84LDD187 pKa = 4.11FGDD190 pKa = 4.03GDD192 pKa = 4.25SLVFAGTTADD202 pKa = 5.46DD203 pKa = 4.21LSADD207 pKa = 3.72QFALSLDD214 pKa = 3.48RR215 pKa = 11.84SVLTKK220 pKa = 10.6TFGDD224 pKa = 3.74EE225 pKa = 4.54FNALSLNNGTSGTWDD240 pKa = 3.87ANFHH244 pKa = 5.85WAPDD248 pKa = 3.67QGSSLPTNGEE258 pKa = 4.13SQWYY262 pKa = 9.11VNPLYY267 pKa = 10.89SPTAGYY273 pKa = 10.89SPFSVSNGALTITAKK288 pKa = 8.95NTPDD292 pKa = 4.65AISDD296 pKa = 3.64AVNGYY301 pKa = 10.8DD302 pKa = 3.97YY303 pKa = 11.62VSGMLNTHH311 pKa = 5.37STFSQTYY318 pKa = 8.57GYY320 pKa = 11.06FEE322 pKa = 4.58MRR324 pKa = 11.84ADD326 pKa = 3.79MPTDD330 pKa = 3.27QATWPAFWLLPEE342 pKa = 5.2DD343 pKa = 4.82GSWPPEE349 pKa = 3.59IDD351 pKa = 3.58VVEE354 pKa = 4.39MRR356 pKa = 11.84GQDD359 pKa = 3.54PNTVHH364 pKa = 6.68VSVHH368 pKa = 5.53SNEE371 pKa = 4.26TGKK374 pKa = 8.36QTTQTSAISVPSTEE388 pKa = 4.56GFHH391 pKa = 6.35TYY393 pKa = 10.26GMLWTEE399 pKa = 4.69EE400 pKa = 4.13EE401 pKa = 4.39IVWYY405 pKa = 10.19FDD407 pKa = 3.49DD408 pKa = 3.92VAIASAEE415 pKa = 4.37TPSDD419 pKa = 3.23MHH421 pKa = 8.3DD422 pKa = 3.32PMYY425 pKa = 11.17LLVNLAVGGAAGKK438 pKa = 9.59PGDD441 pKa = 3.9LSGGAKK447 pKa = 9.03MVIDD451 pKa = 4.49YY452 pKa = 10.67IHH454 pKa = 7.31AYY456 pKa = 9.28EE457 pKa = 4.83INDD460 pKa = 3.67DD461 pKa = 4.02AAPTSSISSASDD473 pKa = 3.21DD474 pKa = 3.76GLVV477 pKa = 3.06

Molecular weight: 50.56 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A0Q5CRR0|A0A0Q5CRR0_9RHIZ Glutamine amidotransferase OS=Rhizobium sp. Leaf306 OX=1736330 GN=ASG19_16100 PE=3 SV=1
MM1 pKa = 6.95NTVTCHH7 pKa = 4.23GHH9 pKa = 5.63TIRR12 pKa = 11.84MLNISGTIWIAAPDD26 pKa = 3.9ALRR29 pKa = 11.84ALGVDD34 pKa = 3.83IARR37 pKa = 11.84KK38 pKa = 8.37GASVHH43 pKa = 5.26LQKK46 pKa = 10.94LPRR49 pKa = 11.84TEE51 pKa = 3.78VRR53 pKa = 11.84LVTSSQYY60 pKa = 10.77PDD62 pKa = 3.76LFAGRR67 pKa = 11.84RR68 pKa = 11.84GNPRR72 pKa = 11.84MNLVTEE78 pKa = 4.04QGLALLRR85 pKa = 11.84EE86 pKa = 4.28QSGKK90 pKa = 10.24FCAGG94 pKa = 3.09

Molecular weight: 10.25 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

4551

0

4551

1439732

26

2831

316.4

34.39

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

11.562 ± 0.047

0.754 ± 0.01

5.859 ± 0.031

5.696 ± 0.033

3.943 ± 0.022

8.348 ± 0.031

1.948 ± 0.016

5.766 ± 0.026

3.792 ± 0.03

9.884 ± 0.044

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.677 ± 0.017

2.821 ± 0.019

4.849 ± 0.024

3.149 ± 0.023

6.42 ± 0.032

6.074 ± 0.031

5.511 ± 0.024

7.431 ± 0.028

1.229 ± 0.015

2.288 ± 0.018
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski