Sidastrum golden leaf spot virus

Taxonomy: Viruses; Monodnaviria; Shotokuvirae; Cressdnaviricota; Repensiviricetes; Geplafuvirales; Geminiviridae; Begomovirus

Average proteome isoelectric point is 9.24

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|E9KZK5|E9KZK5_9GEMI Replication-associated protein OS=Sidastrum golden leaf spot virus OX=858513 GN=AC1 PE=4 SV=1
MM1 pKa = 7.74PSALFQRR8 pKa = 11.84KK9 pKa = 7.51KK10 pKa = 10.21HH11 pKa = 5.52FPNYY15 pKa = 10.03LPSKK19 pKa = 9.07PQLIKK24 pKa = 10.92NISEE28 pKa = 4.31YY29 pKa = 11.05VEE31 pKa = 5.27SSMKK35 pKa = 9.45MGNLIYY41 pKa = 9.98TSCFSSKK48 pKa = 10.33GSSHH52 pKa = 6.35VRR54 pKa = 11.84IADD57 pKa = 3.57SSILNIPAIPMYY69 pKa = 11.22VMEE72 pKa = 4.82NTNHH76 pKa = 6.91ASPPQTPRR84 pKa = 11.84HH85 pKa = 5.1ISRR88 pKa = 11.84RR89 pKa = 11.84TEE91 pKa = 3.21ITANGVVFRR100 pKa = 11.84STEE103 pKa = 3.78DD104 pKa = 3.47LLEE107 pKa = 4.92GGQQTDD113 pKa = 4.14DD114 pKa = 4.32DD115 pKa = 4.44ATTAPLNAALYY126 pKa = 10.49EE127 pKa = 4.1EE128 pKa = 4.7TLLKK132 pKa = 10.54SSKK135 pKa = 10.03PEE137 pKa = 3.27IRR139 pKa = 11.84RR140 pKa = 11.84RR141 pKa = 11.84TLSSYY146 pKa = 11.39HH147 pKa = 6.25NIKK150 pKa = 10.94ANIEE154 pKa = 4.01RR155 pKa = 11.84LFQKK159 pKa = 10.5APEE162 pKa = 4.11PWTPPFQLSSFTRR175 pKa = 11.84VPDD178 pKa = 4.46EE179 pKa = 3.99MQEE182 pKa = 3.85WADD185 pKa = 3.78DD186 pKa = 3.86YY187 pKa = 11.3FGRR190 pKa = 11.84GAAARR195 pKa = 11.84PGKK198 pKa = 8.52TDD200 pKa = 2.96EE201 pKa = 3.78YY202 pKa = 10.18HH203 pKa = 6.07RR204 pKa = 11.84RR205 pKa = 11.84RR206 pKa = 11.84VIVGRR211 pKa = 11.84GRR213 pKa = 11.84QCGLVRR219 pKa = 11.84LAHH222 pKa = 6.69IIILADD228 pKa = 3.08TWTSILGFTQTKK240 pKa = 10.39RR241 pKa = 11.84NITSSMMSHH250 pKa = 6.76RR251 pKa = 11.84NILRR255 pKa = 11.84LSTGKK260 pKa = 9.9NLLGPKK266 pKa = 8.71KK267 pKa = 9.16TGSQIANTEE276 pKa = 4.15SQFKK280 pKa = 10.35LKK282 pKa = 10.48EE283 pKa = 4.19GSHH286 pKa = 5.47QSCSAIQARR295 pKa = 11.84GPAIKK300 pKa = 10.02ISLTKK305 pKa = 10.52RR306 pKa = 11.84KK307 pKa = 8.25MLRR310 pKa = 11.84LKK312 pKa = 10.46SWTLHH317 pKa = 4.46NAKK320 pKa = 10.29FIFLDD325 pKa = 3.49SPLYY329 pKa = 8.71QTSTQDD335 pKa = 3.61CEE337 pKa = 4.47KK338 pKa = 10.45EE339 pKa = 4.41SNSDD343 pKa = 2.94GDD345 pKa = 4.23GFTSSAAAPYY355 pKa = 9.65TSTSTAA361 pKa = 3.19

Molecular weight: 40.4 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|E2GI61|E2GI61_9GEMI Replication enhancer OS=Sidastrum golden leaf spot virus OX=858513 GN=AC3 PE=3 SV=2
MM1 pKa = 7.93IYY3 pKa = 10.96LKK5 pKa = 10.0MPKK8 pKa = 9.05RR9 pKa = 11.84DD10 pKa = 3.64PSWRR14 pKa = 11.84SMAGTSKK21 pKa = 10.59VSRR24 pKa = 11.84SSNFSPRR31 pKa = 11.84GGPKK35 pKa = 9.75VNKK38 pKa = 9.81ASEE41 pKa = 4.12WVNRR45 pKa = 11.84PMYY48 pKa = 9.79RR49 pKa = 11.84KK50 pKa = 8.95PRR52 pKa = 11.84IYY54 pKa = 9.6RR55 pKa = 11.84TLRR58 pKa = 11.84TPDD61 pKa = 3.21VPRR64 pKa = 11.84GCEE67 pKa = 4.34GPCKK71 pKa = 9.32VQSYY75 pKa = 6.98EE76 pKa = 3.66QRR78 pKa = 11.84NDD80 pKa = 2.52ISHH83 pKa = 6.96AGKK86 pKa = 10.32VICISDD92 pKa = 3.57VTRR95 pKa = 11.84GSGITHH101 pKa = 6.35RR102 pKa = 11.84VGKK105 pKa = 9.44RR106 pKa = 11.84FCVKK110 pKa = 9.92SVYY113 pKa = 10.16ILGKK117 pKa = 9.37IWMDD121 pKa = 3.39EE122 pKa = 4.0NIKK125 pKa = 10.61LKK127 pKa = 10.73NHH129 pKa = 5.95TNSVMFWLVRR139 pKa = 11.84DD140 pKa = 3.78RR141 pKa = 11.84RR142 pKa = 11.84PNGTSPWTLVICSTCSTMSLVLQLLRR168 pKa = 11.84MISEE172 pKa = 3.65IVIRR176 pKa = 11.84FNTDD180 pKa = 2.18SMPRR184 pKa = 11.84SLVVNMPAMSRR195 pKa = 11.84PWSGGFGRR203 pKa = 11.84SILMSCITTRR213 pKa = 11.84KK214 pKa = 10.01LLDD217 pKa = 3.08TRR219 pKa = 11.84TIRR222 pKa = 11.84RR223 pKa = 11.84MPSVVHH229 pKa = 5.56GHH231 pKa = 5.4VLMPLIPCMQLFKK244 pKa = 10.76IRR246 pKa = 11.84IYY248 pKa = 10.52FYY250 pKa = 11.18DD251 pKa = 4.08SISII255 pKa = 3.82

Molecular weight: 29.29 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3

0

3

749

133

361

249.7

28.49

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

5.073 ± 1.49

1.736 ± 0.622

3.471 ± 0.432

3.872 ± 0.811

3.738 ± 0.816

5.207 ± 0.524

3.204 ± 1.249

7.343 ± 0.416

5.607 ± 0.911

7.477 ± 0.354

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

4.139 ± 0.965

4.272 ± 0.155

5.207 ± 1.555

3.071 ± 0.797

8.678 ± 1.027

10.814 ± 0.63

7.477 ± 0.682

4.806 ± 1.396

1.736 ± 0.371

3.071 ± 0.499
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski