Aeromonas phage 2_D05

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Myoviridae; unclassified Myoviridae

Average proteome isoelectric point is 6.45

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 77 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A4Y5TXQ9|A0A4Y5TXQ9_9CAUD Uncharacterized protein OS=Aeromonas phage 2_D05 OX=2588098 GN=2D05_004 PE=4 SV=1
MM1 pKa = 7.72DD2 pKa = 4.14TMFLNPTTWDD12 pKa = 3.59LDD14 pKa = 3.3VDD16 pKa = 3.95ASSNIALSSSAYY28 pKa = 10.44SVAQDD33 pKa = 3.4VASQSLLWLGEE44 pKa = 4.02APYY47 pKa = 9.22NTDD50 pKa = 3.71DD51 pKa = 4.97GIPYY55 pKa = 7.94EE56 pKa = 4.12QSVLGQRR63 pKa = 11.84PAQATLAAWYY73 pKa = 8.63QQEE76 pKa = 4.22ALRR79 pKa = 11.84VPDD82 pKa = 3.48VAAATPVLIYY92 pKa = 10.59DD93 pKa = 3.51QARR96 pKa = 11.84GVTGQIKK103 pKa = 8.27VTLTGGTQINVV114 pKa = 3.12

Molecular weight: 12.2 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A4Y5TYX5|A0A4Y5TYX5_9CAUD Uncharacterized protein OS=Aeromonas phage 2_D05 OX=2588098 GN=2D05_025 PE=4 SV=1
MM1 pKa = 6.43KK2 pKa = 9.48TYY4 pKa = 10.46RR5 pKa = 11.84ISGTNRR11 pKa = 11.84RR12 pKa = 11.84TGRR15 pKa = 11.84FEE17 pKa = 3.73TANVQGEE24 pKa = 4.47TPEE27 pKa = 3.96QARR30 pKa = 11.84QLMGMTHH37 pKa = 6.37CRR39 pKa = 11.84MVVLDD44 pKa = 4.06KK45 pKa = 11.35KK46 pKa = 11.11GVV48 pKa = 3.2

Molecular weight: 5.48 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

77

0

77

12737

48

531

165.4

18.2

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

10.591 ± 0.347

1.358 ± 0.134

5.904 ± 0.27

5.998 ± 0.322

3.376 ± 0.168

8.024 ± 0.246

1.507 ± 0.162

5.166 ± 0.206

5.166 ± 0.323

7.828 ± 0.278

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

3.085 ± 0.197

3.769 ± 0.221

4.357 ± 0.196

4.428 ± 0.233

5.527 ± 0.298

6.21 ± 0.26

6.022 ± 0.29

6.76 ± 0.251

1.79 ± 0.113

3.133 ± 0.159
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski