Firmicutes bacterium CAG:56

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Firmicutes; environmental samples

Average proteome isoelectric point is 6.18

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2160 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|R6CGU4|R6CGU4_9FIRM Crispr-protein OS=Firmicutes bacterium CAG:56 OX=1263031 GN=BN708_01819 PE=4 SV=1
MM1 pKa = 7.26KK2 pKa = 10.15KK3 pKa = 10.17KK4 pKa = 10.71VIAGLLGTAMVASLLAGCGSSNNTTDD30 pKa = 3.12TTADD34 pKa = 3.12TTAATEE40 pKa = 3.98AAADD44 pKa = 3.93TTADD48 pKa = 3.53TTADD52 pKa = 3.27TAAADD57 pKa = 4.18AEE59 pKa = 4.61SEE61 pKa = 4.26AQSVADD67 pKa = 3.62AAGDD71 pKa = 3.66VDD73 pKa = 5.73LSNADD78 pKa = 3.6VAVMYY83 pKa = 7.24YY84 pKa = 9.96TYY86 pKa = 11.0GDD88 pKa = 3.58TYY90 pKa = 10.05IASVRR95 pKa = 11.84TALDD99 pKa = 3.21AKK101 pKa = 10.67LDD103 pKa = 3.95EE104 pKa = 5.56LGVKK108 pKa = 8.92YY109 pKa = 10.45QDD111 pKa = 3.17YY112 pKa = 10.55DD113 pKa = 3.77ANNNQTTQNEE123 pKa = 4.42QIDD126 pKa = 4.0TAIQTGANVLIVNIVTSGSVDD147 pKa = 2.99ASSQIVEE154 pKa = 4.21KK155 pKa = 10.89AKK157 pKa = 10.74AADD160 pKa = 3.24IPVIFFNRR168 pKa = 11.84AVEE171 pKa = 4.04GDD173 pKa = 3.51EE174 pKa = 5.93DD175 pKa = 3.74EE176 pKa = 5.2GKK178 pKa = 10.76VLGSYY183 pKa = 9.41DD184 pKa = 2.79KK185 pKa = 11.15CAFVGTDD192 pKa = 3.25APEE195 pKa = 4.49AGHH198 pKa = 5.95MQGEE202 pKa = 4.53MIGNYY207 pKa = 10.2LVDD210 pKa = 4.22NYY212 pKa = 11.46DD213 pKa = 3.74AVDD216 pKa = 3.9LNGDD220 pKa = 3.79GKK222 pKa = 10.56ISYY225 pKa = 10.98AMFMGQLGNVEE236 pKa = 4.61AIYY239 pKa = 8.58RR240 pKa = 11.84TQYY243 pKa = 10.72GVEE246 pKa = 4.18DD247 pKa = 3.83ANKK250 pKa = 9.65VLEE253 pKa = 4.5EE254 pKa = 3.99NGKK257 pKa = 9.65AALEE261 pKa = 4.23YY262 pKa = 10.29FDD264 pKa = 6.02SSNTDD269 pKa = 3.6CYY271 pKa = 11.0QVDD274 pKa = 3.69QDD276 pKa = 4.72GNWSATAANNYY287 pKa = 5.86MTTNLTQYY295 pKa = 11.04NEE297 pKa = 4.08EE298 pKa = 4.08NKK300 pKa = 10.64NMIEE304 pKa = 5.38LVICNNDD311 pKa = 2.77GMAEE315 pKa = 4.13GAISALNDD323 pKa = 3.01KK324 pKa = 10.44GYY326 pKa = 10.98NLGTDD331 pKa = 3.56DD332 pKa = 5.15CKK334 pKa = 10.52TIPVFGVDD342 pKa = 3.08ATDD345 pKa = 3.91AAKK348 pKa = 10.46QLIKK352 pKa = 10.31DD353 pKa = 3.79GKK355 pKa = 7.36MTGTIKK361 pKa = 10.46QDD363 pKa = 3.31AEE365 pKa = 4.21GMADD369 pKa = 4.88CIADD373 pKa = 3.65LTKK376 pKa = 10.89NAGSGQDD383 pKa = 3.33VMANTDD389 pKa = 3.34SYY391 pKa = 11.69NISEE395 pKa = 4.19NVKK398 pKa = 8.84NKK400 pKa = 10.15IYY402 pKa = 10.29IPYY405 pKa = 10.75AMYY408 pKa = 9.87TGEE411 pKa = 4.05EE412 pKa = 4.06

Molecular weight: 43.66 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|R6CHB6|R6CHB6_9FIRM Uncharacterized protein OS=Firmicutes bacterium CAG:56 OX=1263031 GN=BN708_01967 PE=4 SV=1
MM1 pKa = 7.67KK2 pKa = 8.72MTFQPKK8 pKa = 8.86KK9 pKa = 8.2RR10 pKa = 11.84SRR12 pKa = 11.84AKK14 pKa = 9.27VHH16 pKa = 5.81GFRR19 pKa = 11.84ARR21 pKa = 11.84MSTAGGRR28 pKa = 11.84KK29 pKa = 8.62VLAARR34 pKa = 11.84RR35 pKa = 11.84LKK37 pKa = 10.61GRR39 pKa = 11.84KK40 pKa = 8.87KK41 pKa = 10.59LSAA44 pKa = 3.95

Molecular weight: 5.0 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2160

0

2160

707107

29

3033

327.4

36.65

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.882 ± 0.062

1.512 ± 0.027

5.715 ± 0.046

7.701 ± 0.067

3.907 ± 0.04

7.052 ± 0.039

1.707 ± 0.026

6.972 ± 0.056

7.579 ± 0.058

8.523 ± 0.065

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

3.023 ± 0.035

4.219 ± 0.043

3.089 ± 0.028

3.487 ± 0.027

4.261 ± 0.053

5.753 ± 0.055

5.718 ± 0.069

6.923 ± 0.043

0.871 ± 0.017

4.092 ± 0.04
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski