Amino acid dipepetide frequency for Firmicutes bacterium CAG:56

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10^-3.

For more information see sequence space article on Wikipedia.

	Ala	Cys	Asp	Glu	Phe	Gly	His	Ile	Lys	Leu	Met	Asn	Pro	Gln	Arg	Ser	Thr	Val	Trp	Tyr	Xaa
Ala	8.19AlaAla: 8.19 ± 0.148	1.078AlaCys: 1.078 ± 0.046	5.325AlaAsp: 5.325 ± 0.105	6.155AlaGlu: 6.155 ± 0.112	3.229AlaPhe: 3.229 ± 0.086	6.276AlaGly: 6.276 ± 0.108	1.041AlaHis: 1.041 ± 0.043	4.859AlaIle: 4.859 ± 0.091	5.91AlaLys: 5.91 ± 0.105	6.594AlaLeu: 6.594 ± 0.113	2.414AlaMet: 2.414 ± 0.064	2.807AlaAsn: 2.807 ± 0.078	1.976AlaPro: 1.976 ± 0.061	2.396AlaGln: 2.396 ± 0.06	2.712AlaArg: 2.712 ± 0.068	4.24AlaSer: 4.24 ± 0.098	3.526AlaThr: 3.526 ± 0.09	6.46AlaVal: 6.46 ± 0.109	0.683AlaTrp: 0.683 ± 0.031	2.933AlaTyr: 2.933 ± 0.064	0.013AlaXaa: 0.013 ± 0.004
Cys	1.0CysAla: 1.0 ± 0.038	0.359CysCys: 0.359 ± 0.031	0.833CysAsp: 0.833 ± 0.038	0.983CysGlu: 0.983 ± 0.04	0.675CysPhe: 0.675 ± 0.032	1.495CysGly: 1.495 ± 0.055	0.356CysHis: 0.356 ± 0.023	1.158CysIle: 1.158 ± 0.044	0.885CysLys: 0.885 ± 0.041	1.134CysLeu: 1.134 ± 0.039	0.47CysMet: 0.47 ± 0.028	0.501CysAsn: 0.501 ± 0.029	0.557CysPro: 0.557 ± 0.032	0.488CysGln: 0.488 ± 0.028	0.761CysArg: 0.761 ± 0.04	0.933CysSer: 0.933 ± 0.036	0.84CysThr: 0.84 ± 0.035	0.989CysVal: 0.989 ± 0.039	0.119CysTrp: 0.119 ± 0.015	0.58CysTyr: 0.58 ± 0.033	0.001CysXaa: 0.001 ± 0.001
Asp	4.752AspAla: 4.752 ± 0.116	0.772AspCys: 0.772 ± 0.034	2.974AspAsp: 2.974 ± 0.08	4.472AspGlu: 4.472 ± 0.089	2.514AspPhe: 2.514 ± 0.059	4.781AspGly: 4.781 ± 0.098	1.014AspHis: 1.014 ± 0.044	4.155AspIle: 4.155 ± 0.089	3.652AspLys: 3.652 ± 0.079	4.745AspLeu: 4.745 ± 0.087	1.796AspMet: 1.796 ± 0.052	2.029AspAsn: 2.029 ± 0.059	1.834AspPro: 1.834 ± 0.045	1.877AspGln: 1.877 ± 0.056	2.205AspArg: 2.205 ± 0.067	3.035AspSer: 3.035 ± 0.07	3.298AspThr: 3.298 ± 0.076	3.932AspVal: 3.932 ± 0.074	0.518AspTrp: 0.518 ± 0.025	2.793AspTyr: 2.793 ± 0.062	0.006AspXaa: 0.006 ± 0.003
Glu	5.773GluAla: 5.773 ± 0.107	0.839GluCys: 0.839 ± 0.034	4.26GluAsp: 4.26 ± 0.073	7.41GluGlu: 7.41 ± 0.157	2.219GluPhe: 2.219 ± 0.064	4.431GluGly: 4.431 ± 0.096	1.454GluHis: 1.454 ± 0.045	5.626GluIle: 5.626 ± 0.094	7.985GluLys: 7.985 ± 0.13	6.248GluLeu: 6.248 ± 0.099	2.442GluMet: 2.442 ± 0.058	4.302GluAsn: 4.302 ± 0.092	1.667GluPro: 1.667 ± 0.055	3.075GluGln: 3.075 ± 0.074	3.316GluArg: 3.316 ± 0.08	3.608GluSer: 3.608 ± 0.079	4.267GluThr: 4.267 ± 0.112	4.699GluVal: 4.699 ± 0.102	0.563GluTrp: 0.563 ± 0.03	2.823GluTyr: 2.823 ± 0.069	0.003GluXaa: 0.003 ± 0.002
Phe	3.157PheAla: 3.157 ± 0.066	0.861PheCys: 0.861 ± 0.044	2.567PheAsp: 2.567 ± 0.059	2.785PheGlu: 2.785 ± 0.063	1.745PhePhe: 1.745 ± 0.064	2.933PheGly: 2.933 ± 0.072	0.816PheHis: 0.816 ± 0.041	2.229PheIle: 2.229 ± 0.073	1.885PheLys: 1.885 ± 0.055	3.77PheLeu: 3.77 ± 0.093	1.058PheMet: 1.058 ± 0.045	1.257PheAsn: 1.257 ± 0.05	1.24PhePro: 1.24 ± 0.043	1.403PheGln: 1.403 ± 0.042	1.69PheArg: 1.69 ± 0.053	2.623PheSer: 2.623 ± 0.062	2.208PheThr: 2.208 ± 0.057	2.738PheVal: 2.738 ± 0.067	0.406PheTrp: 0.406 ± 0.026	1.696PheTyr: 1.696 ± 0.046	0.003PheXaa: 0.003 ± 0.002
Gly	4.869GlyAla: 4.869 ± 0.103	1.25GlyCys: 1.25 ± 0.046	3.701GlyAsp: 3.701 ± 0.083	4.723GlyGlu: 4.723 ± 0.094	2.949GlyPhe: 2.949 ± 0.064	4.575GlyGly: 4.575 ± 0.087	1.252GlyHis: 1.252 ± 0.04	5.975GlyIle: 5.975 ± 0.108	6.282GlyLys: 6.282 ± 0.114	5.07GlyLeu: 5.07 ± 0.093	2.469GlyMet: 2.469 ± 0.058	3.333GlyAsn: 3.333 ± 0.089	1.151GlyPro: 1.151 ± 0.045	2.077GlyGln: 2.077 ± 0.055	2.913GlyArg: 2.913 ± 0.073	4.408GlySer: 4.408 ± 0.093	4.571GlyThr: 4.571 ± 0.109	4.899GlyVal: 4.899 ± 0.098	0.696GlyTrp: 0.696 ± 0.041	3.346GlyTyr: 3.346 ± 0.07	0.006GlyXaa: 0.006 ± 0.003
His	1.281HisAla: 1.281 ± 0.047	0.31HisCys: 0.31 ± 0.024	0.846HisAsp: 0.846 ± 0.035	1.011HisGlu: 1.011 ± 0.037	0.877HisPhe: 0.877 ± 0.036	1.252HisGly: 1.252 ± 0.046	0.409HisHis: 0.409 ± 0.033	1.383HisIle: 1.383 ± 0.054	0.96HisLys: 0.96 ± 0.04	1.506HisLeu: 1.506 ± 0.055	0.561HisMet: 0.561 ± 0.026	0.666HisAsn: 0.666 ± 0.034	0.847HisPro: 0.847 ± 0.034	0.547HisGln: 0.547 ± 0.031	0.82HisArg: 0.82 ± 0.029	0.935HisSer: 0.935 ± 0.038	0.948HisThr: 0.948 ± 0.036	1.025HisVal: 1.025 ± 0.033	0.12HisTrp: 0.12 ± 0.014	0.764HisTyr: 0.764 ± 0.036	0.0HisXaa: 0.0 ± 0.0
Ile	5.573IleAla: 5.573 ± 0.097	1.407IleCys: 1.407 ± 0.055	3.707IleAsp: 3.707 ± 0.084	4.51IleGlu: 4.51 ± 0.093	2.66IlePhe: 2.66 ± 0.074	4.94IleGly: 4.94 ± 0.095	1.344IleHis: 1.344 ± 0.048	4.171IleIle: 4.171 ± 0.111	4.08IleLys: 4.08 ± 0.077	6.979IleLeu: 6.979 ± 0.137	1.795IleMet: 1.795 ± 0.057	2.513IleAsn: 2.513 ± 0.069	2.942IlePro: 2.942 ± 0.071	2.325IleGln: 2.325 ± 0.065	3.896IleArg: 3.896 ± 0.098	4.593IleSer: 4.593 ± 0.083	4.267IleThr: 4.267 ± 0.093	4.671IleVal: 4.671 ± 0.087	0.615IleTrp: 0.615 ± 0.031	2.735IleTyr: 2.735 ± 0.067	0.003IleXaa: 0.003 ± 0.002
Lys	6.077LysAla: 6.077 ± 0.119	0.803LysCys: 0.803 ± 0.039	4.203LysAsp: 4.203 ± 0.075	7.319LysGlu: 7.319 ± 0.111	1.959LysPhe: 1.959 ± 0.056	4.559LysGly: 4.559 ± 0.087	1.018LysHis: 1.018 ± 0.039	5.3LysIle: 5.3 ± 0.093	7.934LysLys: 7.934 ± 0.143	5.674LysLeu: 5.674 ± 0.091	2.359LysMet: 2.359 ± 0.06	4.093LysAsn: 4.093 ± 0.08	1.908LysPro: 1.908 ± 0.05	2.643LysGln: 2.643 ± 0.067	3.158LysArg: 3.158 ± 0.084	3.608LysSer: 3.608 ± 0.076	4.39LysThr: 4.39 ± 0.09	5.087LysVal: 5.087 ± 0.112	0.666LysTrp: 0.666 ± 0.032	3.031LysTyr: 3.031 ± 0.067	0.001LysXaa: 0.001 ± 0.002
Leu	6.713LeuAla: 6.713 ± 0.119	1.455LeuCys: 1.455 ± 0.046	4.972LeuAsp: 4.972 ± 0.093	6.283LeuGlu: 6.283 ± 0.11	3.569LeuPhe: 3.569 ± 0.09	5.456LeuGly: 5.456 ± 0.097	1.52LeuHis: 1.52 ± 0.05	5.383LeuIle: 5.383 ± 0.113	6.432LeuLys: 6.432 ± 0.105	7.866LeuLeu: 7.866 ± 0.184	2.472LeuMet: 2.472 ± 0.066	3.562LeuAsn: 3.562 ± 0.066	3.224LeuPro: 3.224 ± 0.073	3.022LeuGln: 3.022 ± 0.069	3.507LeuArg: 3.507 ± 0.085	5.64LeuSer: 5.64 ± 0.105	5.08LeuThr: 5.08 ± 0.083	5.139LeuVal: 5.139 ± 0.088	0.716LeuTrp: 0.716 ± 0.034	3.212LeuTyr: 3.212 ± 0.073	0.01LeuXaa: 0.01 ± 0.004
Met	2.475MetAla: 2.475 ± 0.064	0.33MetCys: 0.33 ± 0.022	1.874MetAsp: 1.874 ± 0.056	2.473MetGlu: 2.473 ± 0.074	1.007MetPhe: 1.007 ± 0.043	1.967MetGly: 1.967 ± 0.059	0.503MetHis: 0.503 ± 0.031	2.099MetIle: 2.099 ± 0.061	2.514MetLys: 2.514 ± 0.057	2.676MetLeu: 2.676 ± 0.067	0.945MetMet: 0.945 ± 0.044	1.344MetAsn: 1.344 ± 0.045	1.121MetPro: 1.121 ± 0.042	1.336MetGln: 1.336 ± 0.047	1.22MetArg: 1.22 ± 0.041	1.601MetSer: 1.601 ± 0.046	1.768MetThr: 1.768 ± 0.053	1.881MetVal: 1.881 ± 0.046	0.206MetTrp: 0.206 ± 0.017	0.887MetTyr: 0.887 ± 0.033	0.001MetXaa: 0.001 ± 0.001
Asn	3.387AsnAla: 3.387 ± 0.086	0.591AsnCys: 0.591 ± 0.03	2.049AsnAsp: 2.049 ± 0.059	2.589AsnGlu: 2.589 ± 0.066	1.383AsnPhe: 1.383 ± 0.045	3.762AsnGly: 3.762 ± 0.088	0.748AsnHis: 0.748 ± 0.036	3.175AsnIle: 3.175 ± 0.062	2.555AsnLys: 2.555 ± 0.062	3.797AsnLeu: 3.797 ± 0.076	1.342AsnMet: 1.342 ± 0.042	1.824AsnAsn: 1.824 ± 0.071	1.915AsnPro: 1.915 ± 0.052	1.489AsnGln: 1.489 ± 0.044	1.936AsnArg: 1.936 ± 0.053	2.232AsnSer: 2.232 ± 0.064	2.417AsnThr: 2.417 ± 0.071	2.872AsnVal: 2.872 ± 0.073	0.412AsnTrp: 0.412 ± 0.023	1.714AsnTyr: 1.714 ± 0.056	0.001AsnXaa: 0.001 ± 0.001
Pro	2.346ProAla: 2.346 ± 0.061	0.458ProCys: 0.458 ± 0.028	2.213ProAsp: 2.213 ± 0.065	3.009ProGlu: 3.009 ± 0.077	1.376ProPhe: 1.376 ± 0.045	2.16ProGly: 2.16 ± 0.064	0.511ProHis: 0.511 ± 0.026	1.78ProIle: 1.78 ± 0.051	2.09ProLys: 2.09 ± 0.056	2.434ProLeu: 2.434 ± 0.065	0.832ProMet: 0.832 ± 0.036	1.205ProAsn: 1.205 ± 0.044	0.622ProPro: 0.622 ± 0.032	1.266ProGln: 1.266 ± 0.039	0.851ProArg: 0.851 ± 0.039	1.778ProSer: 1.778 ± 0.049	1.525ProThr: 1.525 ± 0.055	2.816ProVal: 2.816 ± 0.066	0.296ProTrp: 0.296 ± 0.023	1.314ProTyr: 1.314 ± 0.042	0.004ProXaa: 0.004 ± 0.002
Gln	2.605GlnAla: 2.605 ± 0.07	0.382GlnCys: 0.382 ± 0.026	1.727GlnAsp: 1.727 ± 0.052	2.942GlnGlu: 2.942 ± 0.077	1.236GlnPhe: 1.236 ± 0.041	2.001GlnGly: 2.001 ± 0.06	0.496GlnHis: 0.496 ± 0.027	2.954GlnIle: 2.954 ± 0.069	3.476GlnLys: 3.476 ± 0.079	2.623GlnLeu: 2.623 ± 0.07	1.25GlnMet: 1.25 ± 0.037	1.737GlnAsn: 1.737 ± 0.047	0.902GlnPro: 0.902 ± 0.038	1.311GlnGln: 1.311 ± 0.051	1.38GlnArg: 1.38 ± 0.045	1.796GlnSer: 1.796 ± 0.06	2.041GlnThr: 2.041 ± 0.056	2.336GlnVal: 2.336 ± 0.054	0.29GlnTrp: 0.29 ± 0.021	1.382GlnTyr: 1.382 ± 0.045	0.003GlnXaa: 0.003 ± 0.002
Arg	2.715ArgAla: 2.715 ± 0.073	0.615ArgCys: 0.615 ± 0.035	2.076ArgAsp: 2.076 ± 0.057	3.635ArgGlu: 3.635 ± 0.098	1.752ArgPhe: 1.752 ± 0.05	2.326ArgGly: 2.326 ± 0.065	0.762ArgHis: 0.762 ± 0.034	3.479ArgIle: 3.479 ± 0.076	3.892ArgLys: 3.892 ± 0.084	3.511ArgLeu: 3.511 ± 0.075	1.52ArgMet: 1.52 ± 0.048	1.896ArgAsn: 1.896 ± 0.054	1.257ArgPro: 1.257 ± 0.042	1.765ArgGln: 1.765 ± 0.057	2.205ArgArg: 2.205 ± 0.075	2.294ArgSer: 2.294 ± 0.058	2.239ArgThr: 2.239 ± 0.063	2.53ArgVal: 2.53 ± 0.065	0.358ArgTrp: 0.358 ± 0.024	1.778ArgTyr: 1.778 ± 0.045	0.001ArgXaa: 0.001 ± 0.001
Ser	4.475SerAla: 4.475 ± 0.107	0.898SerCys: 0.898 ± 0.048	3.511SerAsp: 3.511 ± 0.077	4.294SerGlu: 4.294 ± 0.088	2.551SerPhe: 2.551 ± 0.074	5.172SerGly: 5.172 ± 0.096	0.861SerHis: 0.861 ± 0.037	3.81SerIle: 3.81 ± 0.08	3.414SerLys: 3.414 ± 0.075	4.691SerLeu: 4.691 ± 0.09	1.785SerMet: 1.785 ± 0.052	2.126SerAsn: 2.126 ± 0.07	1.488SerPro: 1.488 ± 0.047	1.769SerGln: 1.769 ± 0.055	2.604SerArg: 2.604 ± 0.057	3.588SerSer: 3.588 ± 0.089	3.018SerThr: 3.018 ± 0.078	4.451SerVal: 4.451 ± 0.101	0.57SerTrp: 0.57 ± 0.03	2.447SerTyr: 2.447 ± 0.062	0.007SerXaa: 0.007 ± 0.003
Thr	5.207ThrAla: 5.207 ± 0.111	0.734ThrCys: 0.734 ± 0.044	3.427ThrAsp: 3.427 ± 0.081	4.507ThrGlu: 4.507 ± 0.098	2.287ThrPhe: 2.287 ± 0.064	4.921ThrGly: 4.921 ± 0.099	0.786ThrHis: 0.786 ± 0.034	3.78ThrIle: 3.78 ± 0.077	3.482ThrLys: 3.482 ± 0.083	4.895ThrLeu: 4.895 ± 0.087	1.414ThrMet: 1.414 ± 0.049	1.983ThrAsn: 1.983 ± 0.06	2.307ThrPro: 2.307 ± 0.065	1.701ThrGln: 1.701 ± 0.053	1.87ThrArg: 1.87 ± 0.051	3.149ThrSer: 3.149 ± 0.077	3.056ThrThr: 3.056 ± 0.084	4.917ThrVal: 4.917 ± 0.121	0.557ThrTrp: 0.557 ± 0.03	2.196ThrTyr: 2.196 ± 0.067	0.004ThrXaa: 0.004 ± 0.002
Val	4.678ValAla: 4.678 ± 0.093	1.239ValCys: 1.239 ± 0.052	3.752ValAsp: 3.752 ± 0.084	4.483ValGlu: 4.483 ± 0.092	2.984ValPhe: 2.984 ± 0.071	4.113ValGly: 4.113 ± 0.086	1.119ValHis: 1.119 ± 0.039	5.121ValIle: 5.121 ± 0.09	5.148ValLys: 5.148 ± 0.108	6.507ValLeu: 6.507 ± 0.112	1.964ValMet: 1.964 ± 0.058	2.831ValAsn: 2.831 ± 0.079	2.394ValPro: 2.394 ± 0.06	2.253ValGln: 2.253 ± 0.058	2.985ValArg: 2.985 ± 0.076	4.745ValSer: 4.745 ± 0.09	4.852ValThr: 4.852 ± 0.129	4.728ValVal: 4.728 ± 0.094	0.547ValTrp: 0.547 ± 0.027	2.786ValTyr: 2.786 ± 0.058	0.003ValXaa: 0.003 ± 0.002
Trp	0.533TrpAla: 0.533 ± 0.031	0.123TrpCys: 0.123 ± 0.014	0.553TrpAsp: 0.553 ± 0.028	0.577TrpGlu: 0.577 ± 0.028	0.38TrpPhe: 0.38 ± 0.028	0.544TrpGly: 0.544 ± 0.029	0.199TrpHis: 0.199 ± 0.018	0.707TrpIle: 0.707 ± 0.032	0.837TrpLys: 0.837 ± 0.038	0.8TrpLeu: 0.8 ± 0.037	0.305TrpMet: 0.305 ± 0.023	0.451TrpAsn: 0.451 ± 0.025	0.165TrpPro: 0.165 ± 0.016	0.348TrpGln: 0.348 ± 0.024	0.351TrpArg: 0.351 ± 0.026	0.481TrpSer: 0.481 ± 0.028	0.406TrpThr: 0.406 ± 0.03	0.458TrpVal: 0.458 ± 0.024	0.072TrpTrp: 0.072 ± 0.011	0.42TrpTyr: 0.42 ± 0.024	0.001TrpXaa: 0.001 ± 0.002
Tyr	2.997TyrAla: 2.997 ± 0.062	0.612TyrCys: 0.612 ± 0.032	2.581TyrAsp: 2.581 ± 0.067	2.845TyrGlu: 2.845 ± 0.068	1.714TyrPhe: 1.714 ± 0.045	2.843TyrGly: 2.843 ± 0.068	0.857TyrHis: 0.857 ± 0.038	2.581TyrIle: 2.581 ± 0.069	2.376TyrLys: 2.376 ± 0.069	3.707TyrLeu: 3.707 ± 0.083	1.031TyrMet: 1.031 ± 0.042	1.728TyrAsn: 1.728 ± 0.056	1.362TyrPro: 1.362 ± 0.048	1.778TyrGln: 1.778 ± 0.053	2.216TyrArg: 2.216 ± 0.055	2.232TyrSer: 2.232 ± 0.059	2.468TyrThr: 2.468 ± 0.075	2.604TyrVal: 2.604 ± 0.059	0.305TyrTrp: 0.305 ± 0.02	2.086TyrTyr: 2.086 ± 0.07	0.0TyrXaa: 0.0 ± 0.0
Xaa	0.013XaaAla: 0.013 ± 0.004	0.0XaaCys: 0.0 ± 0.0	0.003XaaAsp: 0.003 ± 0.002	0.003XaaGlu: 0.003 ± 0.002	0.003XaaPhe: 0.003 ± 0.002	0.01XaaGly: 0.01 ± 0.003	0.001XaaHis: 0.001 ± 0.001	0.0XaaIle: 0.0 ± 0.0	0.0XaaLys: 0.0 ± 0.0	0.007XaaLeu: 0.007 ± 0.003	0.003XaaMet: 0.003 ± 0.002	0.003XaaAsn: 0.003 ± 0.002	0.004XaaPro: 0.004 ± 0.002	0.001XaaGln: 0.001 ± 0.001	0.01XaaArg: 0.01 ± 0.004	0.01XaaSer: 0.01 ± 0.004	0.0XaaThr: 0.0 ± 0.0	0.0XaaVal: 0.0 ± 0.0	0.0XaaTrp: 0.0 ± 0.0	0.001XaaTyr: 0.001 ± 0.001	0.074XaaXaa: 0.074 ± 0.013

Statistics based on 2160 proteins (707107 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944

Contact: Lukasz P. Kozlowski

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