Odonata associated gemycircularvirus-1
Average proteome isoelectric point is 7.13
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 2 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A0B4UHX2|A0A0B4UHX2_9VIRU Replication-associated protein OS=Odonata associated gemycircularvirus-1 OX=1592107 PE=3 SV=1
MM1 pKa = 8.06 PSFCTNARR9 pKa = 11.84 HH10 pKa = 6.38 FLITYY15 pKa = 7.25 AQCGDD20 pKa = 3.45 LSEE23 pKa = 4.37 WNVLDD28 pKa = 4.16 KK29 pKa = 11.03 FSSLGAEE36 pKa = 4.22 CIIARR41 pKa = 11.84 EE42 pKa = 3.95 YY43 pKa = 10.86 HH44 pKa = 6.71 ADD46 pKa = 3.65 LGLHH50 pKa = 5.8 LHH52 pKa = 5.84 VFVDD56 pKa = 4.55 FGRR59 pKa = 11.84 KK60 pKa = 8.01 FRR62 pKa = 11.84 SRR64 pKa = 11.84 KK65 pKa = 7.67 TDD67 pKa = 2.7 IFDD70 pKa = 3.24 VDD72 pKa = 3.54 GRR74 pKa = 11.84 HH75 pKa = 6.05 PNIKK79 pKa = 9.98 RR80 pKa = 11.84 SWGTPEE86 pKa = 4.27 KK87 pKa = 10.76 GYY89 pKa = 10.97 DD90 pKa = 3.43 YY91 pKa = 10.61 AIKK94 pKa = 10.61 DD95 pKa = 3.82 GEE97 pKa = 4.57 VVAGGLEE104 pKa = 4.2 RR105 pKa = 11.84 PDD107 pKa = 3.76 GVHH110 pKa = 7.12 GSGKK114 pKa = 8.36 TFDD117 pKa = 4.05 LWSQVAGAEE126 pKa = 4.16 DD127 pKa = 3.75 RR128 pKa = 11.84 GEE130 pKa = 3.89 FWRR133 pKa = 11.84 LVEE136 pKa = 4.16 EE137 pKa = 4.93 LDD139 pKa = 4.72 PKK141 pKa = 10.92 ALCCSFTQLSKK152 pKa = 10.32 FADD155 pKa = 2.99 WRR157 pKa = 11.84 FAEE160 pKa = 4.52 VVADD164 pKa = 3.79 YY165 pKa = 9.21 EE166 pKa = 4.75 SPGGIGFVPGDD177 pKa = 3.31 VDD179 pKa = 5.71 GRR181 pKa = 11.84 DD182 pKa = 3.3 SWVEE186 pKa = 3.83 SAGLGLGEE194 pKa = 4.86 PPVGMLLTPTLEE206 pKa = 4.51 GGNGSGQGPYY216 pKa = 9.58 PPPSLVVGASGVWYY230 pKa = 10.46 ADD232 pKa = 3.22 VFLVDD237 pKa = 4.41 LVLGVHH243 pKa = 6.78 EE244 pKa = 4.33 STPRR248 pKa = 11.84 QVCYY252 pKa = 10.51 FDD254 pKa = 5.93 DD255 pKa = 3.42 IRR257 pKa = 11.84 GGIKK261 pKa = 9.97 FFPAFKK267 pKa = 9.81 EE268 pKa = 4.05 WLGGQQTVSVKK279 pKa = 10.36 RR280 pKa = 11.84 LYY282 pKa = 10.48 RR283 pKa = 11.84 DD284 pKa = 3.36 PKK286 pKa = 9.31 LVRR289 pKa = 11.84 WGKK292 pKa = 8.77 PSIWISNDD300 pKa = 3.18 DD301 pKa = 4.01 PRR303 pKa = 11.84 HH304 pKa = 5.68 SMEE307 pKa = 4.88 PSDD310 pKa = 4.3 ISWMEE315 pKa = 3.47 ANARR319 pKa = 11.84 FIEE322 pKa = 4.16 VRR324 pKa = 11.84 EE325 pKa = 4.37 AIYY328 pKa = 9.36 FSCQQ332 pKa = 2.77
Molecular weight: 36.98 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 4.977
IPC2_protein 5.016
IPC_protein 4.978
Toseland 4.902
ProMoST 5.118
Dawson 4.978
Bjellqvist 5.105
Wikipedia 4.863
Rodwell 4.876
Grimsley 4.825
Solomon 4.978
Lehninger 4.927
Nozaki 5.092
DTASelect 5.283
Thurlkill 4.914
EMBOSS 4.902
Sillero 5.156
Patrickios 3.973
IPC_peptide 4.978
IPC2_peptide 5.156
IPC2.peptide.svr19 5.11
Protein with the highest isoelectric point:
>tr|A0A0B4UHX2|A0A0B4UHX2_9VIRU Replication-associated protein OS=Odonata associated gemycircularvirus-1 OX=1592107 PE=3 SV=1
MM1 pKa = 7.98 GYY3 pKa = 10.2 RR4 pKa = 11.84 GGPTKK9 pKa = 10.48 RR10 pKa = 11.84 ALAAYY15 pKa = 9.03 KK16 pKa = 10.17 RR17 pKa = 11.84 SQSSKK22 pKa = 9.78 RR23 pKa = 11.84 RR24 pKa = 11.84 YY25 pKa = 8.66 SSKK28 pKa = 10.51 GKK30 pKa = 6.44 TRR32 pKa = 11.84 RR33 pKa = 11.84 YY34 pKa = 9.06 RR35 pKa = 11.84 KK36 pKa = 7.99 KK37 pKa = 8.24 TKK39 pKa = 9.72 RR40 pKa = 11.84 SYY42 pKa = 10.53 RR43 pKa = 11.84 RR44 pKa = 11.84 PAMTRR49 pKa = 11.84 KK50 pKa = 9.83 SLLNVTSRR58 pKa = 11.84 KK59 pKa = 9.78 KK60 pKa = 9.82 QDD62 pKa = 3.1 NMLSFSNTNGSGASQALAAGSLFVNGSTGYY92 pKa = 11.26 AMSLFCPTARR102 pKa = 11.84 SLVTAGATNTIVDD115 pKa = 3.88 TADD118 pKa = 3.55 RR119 pKa = 11.84 TSTTCYY125 pKa = 9.72 LRR127 pKa = 11.84 GYY129 pKa = 10.39 RR130 pKa = 11.84 EE131 pKa = 4.32 DD132 pKa = 5.31 LRR134 pKa = 11.84 IQTSSPLPWLWRR146 pKa = 11.84 RR147 pKa = 11.84 ICFTSKK153 pKa = 10.69 GATFQQAFNDD163 pKa = 4.01 SSPTAPIQPYY173 pKa = 9.49 SDD175 pKa = 3.37 TSIGMARR182 pKa = 11.84 KK183 pKa = 8.61 WFNLQVNNSPNTVANYY199 pKa = 8.27 NQIMFRR205 pKa = 11.84 GNFGADD211 pKa = 2.86 WNDD214 pKa = 3.24 VITAKK219 pKa = 10.47 VDD221 pKa = 3.28 TTRR224 pKa = 11.84 ITVKK228 pKa = 9.88 SDD230 pKa = 2.25 RR231 pKa = 11.84 VTRR234 pKa = 11.84 ITTGNNSGHH243 pKa = 5.48 FSEE246 pKa = 5.23 RR247 pKa = 11.84 KK248 pKa = 8.77 LWYY251 pKa = 9.46 PMNRR255 pKa = 11.84 NLVYY259 pKa = 10.9 DD260 pKa = 4.26 DD261 pKa = 5.25 DD262 pKa = 4.88 EE263 pKa = 6.24 NGAAEE268 pKa = 4.29 TPQYY272 pKa = 11.0 VSSDD276 pKa = 3.13 AKK278 pKa = 10.84 AAWGDD283 pKa = 3.92 YY284 pKa = 10.64 YY285 pKa = 10.41 IVDD288 pKa = 3.78 YY289 pKa = 10.59 LVPGVGGTTSDD300 pKa = 3.93 VVNLNCTATLYY311 pKa = 9.4 WHH313 pKa = 7.01 EE314 pKa = 4.29 KK315 pKa = 9.3
Molecular weight: 35.25 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.278
IPC2_protein 9.604
IPC_protein 9.94
Toseland 10.058
ProMoST 9.867
Dawson 10.306
Bjellqvist 10.028
Wikipedia 10.511
Rodwell 10.599
Grimsley 10.394
Solomon 10.335
Lehninger 10.292
Nozaki 10.072
DTASelect 10.014
Thurlkill 10.145
EMBOSS 10.482
Sillero 10.218
Patrickios 9.853
IPC_peptide 10.321
IPC2_peptide 8.887
IPC2.peptide.svr19 8.587
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
2
0
2
647
315
332
323.5
36.12
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
7.11 ± 0.804
1.7 ± 0.302
6.646 ± 0.878
4.019 ± 1.708
4.637 ± 1.027
9.119 ± 1.5
1.546 ± 0.64
3.709 ± 0.376
4.946 ± 0.54
6.646 ± 0.655
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
1.7 ± 0.367
4.328 ± 1.866
4.946 ± 0.798
2.782 ± 0.276
7.264 ± 0.918
8.501 ± 0.942
6.646 ± 2.691
6.801 ± 0.986
2.782 ± 0.393
4.173 ± 0.86
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here