Mongoose feces-associated gemycircularvirus d

Taxonomy: Viruses; Monodnaviria; Shotokuvirae; Cressdnaviricota; Repensiviricetes; Geplafuvirales; Genomoviridae; Gemycircularvirus; Gemycircularvirus monas1; Mongoose associated gemycircularvirus 1

Average proteome isoelectric point is 7.45

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A0E3M0E2|A0A0E3M0E2_9VIRU Replication-associated protein OS=Mongoose feces-associated gemycircularvirus d OX=1634487 PE=3 SV=1
MM1 pKa = 7.4LFVNSRR7 pKa = 11.84YY8 pKa = 10.16LLLTYY13 pKa = 10.06AQSNGLDD20 pKa = 3.1EE21 pKa = 4.66WDD23 pKa = 3.83VSNHH27 pKa = 5.62LSTLGAEE34 pKa = 4.52CIIARR39 pKa = 11.84EE40 pKa = 4.02NHH42 pKa = 6.94DD43 pKa = 4.23DD44 pKa = 3.98GGTHH48 pKa = 6.28LHH50 pKa = 6.56CFVDD54 pKa = 4.87FNRR57 pKa = 11.84KK58 pKa = 8.32FRR60 pKa = 11.84SRR62 pKa = 11.84NVRR65 pKa = 11.84VFDD68 pKa = 3.66VGGFHH73 pKa = 7.41PNISPSRR80 pKa = 11.84GTPEE84 pKa = 3.69KK85 pKa = 10.76GYY87 pKa = 10.89DD88 pKa = 3.47YY89 pKa = 10.69AIKK92 pKa = 10.87DD93 pKa = 3.59GDD95 pKa = 4.09VVAGGLDD102 pKa = 3.28RR103 pKa = 11.84PAPRR107 pKa = 11.84GGMHH111 pKa = 6.28VGAHH115 pKa = 5.22RR116 pKa = 11.84VSNVAHH122 pKa = 6.47LCEE125 pKa = 4.38SSEE128 pKa = 4.2EE129 pKa = 3.9FLEE132 pKa = 5.89LYY134 pKa = 10.95DD135 pKa = 3.74EE136 pKa = 4.52MEE138 pKa = 4.76RR139 pKa = 11.84GDD141 pKa = 4.1LIGRR145 pKa = 11.84FSNVRR150 pKa = 11.84AYY152 pKa = 10.73ADD154 pKa = 2.91WRR156 pKa = 11.84FRR158 pKa = 11.84LDD160 pKa = 2.95IPEE163 pKa = 4.3YY164 pKa = 8.34STPSEE169 pKa = 4.23FVFSSGEE176 pKa = 3.65LDD178 pKa = 5.18GRR180 pKa = 11.84DD181 pKa = 3.29DD182 pKa = 3.61WVQQSGVGSGEE193 pKa = 3.9PLIGMSFTSVRR204 pKa = 11.84LQRR207 pKa = 11.84KK208 pKa = 8.9SLVLYY213 pKa = 10.28GRR215 pKa = 11.84SLTGKK220 pKa = 5.78TTWARR225 pKa = 11.84SLGSHH230 pKa = 6.5MYY232 pKa = 9.84SEE234 pKa = 4.75RR235 pKa = 11.84RR236 pKa = 11.84LNAQMADD243 pKa = 3.72DD244 pKa = 4.51MEE246 pKa = 4.78HH247 pKa = 5.58TAHH250 pKa = 6.19YY251 pKa = 10.15HH252 pKa = 5.36VLDD255 pKa = 4.73DD256 pKa = 3.28VDD258 pKa = 4.94LRR260 pKa = 11.84YY261 pKa = 9.84FPAWKK266 pKa = 9.64SWLGGMQWISNEE278 pKa = 3.51LKK280 pKa = 10.0YY281 pKa = 11.12RR282 pKa = 11.84NVRR285 pKa = 11.84LLKK288 pKa = 9.88WGRR291 pKa = 11.84PCIWCNNTDD300 pKa = 3.18PRR302 pKa = 11.84DD303 pKa = 3.56VMRR306 pKa = 11.84RR307 pKa = 11.84SMAAFDD313 pKa = 3.87GQGDD317 pKa = 4.1GKK319 pKa = 10.74FSHH322 pKa = 7.21EE323 pKa = 3.81DD324 pKa = 3.68LAWLEE329 pKa = 4.13ANCVFIHH336 pKa = 6.53IDD338 pKa = 3.38EE339 pKa = 5.27EE340 pKa = 4.36IATFRR345 pKa = 11.84ASTTT349 pKa = 3.32

Molecular weight: 39.78 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A0E3M0E2|A0A0E3M0E2_9VIRU Replication-associated protein OS=Mongoose feces-associated gemycircularvirus d OX=1634487 PE=3 SV=1
MM1 pKa = 7.74PPRR4 pKa = 11.84YY5 pKa = 9.21HH6 pKa = 6.29NRR8 pKa = 11.84RR9 pKa = 11.84RR10 pKa = 11.84TIGVSRR16 pKa = 11.84RR17 pKa = 11.84RR18 pKa = 11.84KK19 pKa = 8.07FTSSRR24 pKa = 11.84YY25 pKa = 8.4RR26 pKa = 11.84KK27 pKa = 9.0PRR29 pKa = 11.84RR30 pKa = 11.84ATRR33 pKa = 11.84RR34 pKa = 11.84AYY36 pKa = 9.88RR37 pKa = 11.84KK38 pKa = 8.87PIRR41 pKa = 11.84RR42 pKa = 11.84VSRR45 pKa = 11.84RR46 pKa = 11.84RR47 pKa = 11.84MINMLSKK54 pKa = 10.66KK55 pKa = 10.3KK56 pKa = 10.0RR57 pKa = 11.84DD58 pKa = 3.6TMLSSAGSGVNPPPDD73 pKa = 3.64STPTGSSIIISPFTSTTNLFEE94 pKa = 4.76SGVHH98 pKa = 5.76SLIFTPSMKK107 pKa = 10.35PLTPSNATFVAQRR120 pKa = 11.84TNTYY124 pKa = 10.12CYY126 pKa = 10.15YY127 pKa = 10.76KK128 pKa = 10.85GFAEE132 pKa = 5.08TYY134 pKa = 9.64TFLPNDD140 pKa = 3.36NSVWWHH146 pKa = 4.82RR147 pKa = 11.84RR148 pKa = 11.84IVFSSRR154 pKa = 11.84RR155 pKa = 11.84RR156 pKa = 11.84FAEE159 pKa = 3.79LNQITSGSGTLAPAVTAGGISRR181 pKa = 11.84RR182 pKa = 11.84KK183 pKa = 9.16FKK185 pKa = 11.19DD186 pKa = 3.05MSEE189 pKa = 4.13TSGLDD194 pKa = 3.25GFNDD198 pKa = 3.17IQALIIAEE206 pKa = 4.31VFRR209 pKa = 11.84GAYY212 pKa = 7.23NTDD215 pKa = 2.67WADD218 pKa = 3.62PLRR221 pKa = 11.84ARR223 pKa = 11.84LDD225 pKa = 3.43TTKK228 pKa = 10.6INVHH232 pKa = 5.55SDD234 pKa = 2.89RR235 pKa = 11.84LVTLRR240 pKa = 11.84SGNDD244 pKa = 3.01VPAPRR249 pKa = 11.84IVKK252 pKa = 10.0HH253 pKa = 4.85YY254 pKa = 9.66TPINKK259 pKa = 9.01TIVYY263 pKa = 9.39DD264 pKa = 3.79DD265 pKa = 4.21EE266 pKa = 5.1EE267 pKa = 5.73IGVGMQSSYY276 pKa = 11.31YY277 pKa = 10.32AVQGKK282 pKa = 9.03PGIGDD287 pKa = 4.43LYY289 pKa = 11.24VLDD292 pKa = 5.18FFEE295 pKa = 5.71CPVPNDD301 pKa = 3.55TTTTTLNISSQFTSYY316 pKa = 7.21WHH318 pKa = 6.48EE319 pKa = 4.05KK320 pKa = 9.6

Molecular weight: 36.36 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2

0

2

669

320

349

334.5

38.07

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

5.68 ± 0.264

1.196 ± 0.409

6.577 ± 1.13

4.484 ± 1.198

4.783 ± 0.069

7.474 ± 1.101

2.99 ± 0.799

4.783 ± 1.051

3.587 ± 0.789

7.025 ± 1.228

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.541 ± 0.253

4.634 ± 0.263

4.783 ± 1.275

1.943 ± 0.049

9.567 ± 0.759

9.118 ± 0.632

6.876 ± 2.239

5.979 ± 0.254

2.093 ± 0.604

3.886 ± 0.35
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski