Changjiang tombus-like virus 22

Taxonomy: Viruses; Riboviria; unclassified Riboviria; unclassified RNA viruses ShiM-2016

Average proteome isoelectric point is 5.44

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1L3KFX0|A0A1L3KFX0_9VIRU Uncharacterized protein OS=Changjiang tombus-like virus 22 OX=1922816 PE=4 SV=1
MM1 pKa = 7.57EE2 pKa = 6.37RR3 pKa = 11.84GDD5 pKa = 4.13CDD7 pKa = 5.43LIVPEE12 pKa = 5.02LTADD16 pKa = 3.72DD17 pKa = 4.12NSINRR22 pKa = 11.84PAVGAAFGSFDD33 pKa = 3.62YY34 pKa = 11.72GEE36 pKa = 4.59FSLFSLGLRR45 pKa = 11.84SASRR49 pKa = 11.84LARR52 pKa = 11.84HH53 pKa = 5.98GAVLDD58 pKa = 3.87EE59 pKa = 4.53RR60 pKa = 11.84VGEE63 pKa = 4.28AGSVAGSGDD72 pKa = 3.73LVGDD76 pKa = 3.38SDD78 pKa = 3.76YY79 pKa = 10.91HH80 pKa = 7.94YY81 pKa = 10.69EE82 pKa = 4.16VNSPGWVDD90 pKa = 3.92LSPEE94 pKa = 4.06RR95 pKa = 11.84EE96 pKa = 4.18EE97 pKa = 5.15QSGLDD102 pKa = 3.53PSVPPPAEE110 pKa = 4.13WEE112 pKa = 4.32WVDD115 pKa = 4.26PMSDD119 pKa = 3.56GPDD122 pKa = 3.52GYY124 pKa = 11.55SRR126 pKa = 11.84LPNGEE131 pKa = 4.09IVWNGAEE138 pKa = 4.11NYY140 pKa = 8.28AALMARR146 pKa = 11.84DD147 pKa = 4.63LNHH150 pKa = 6.51EE151 pKa = 4.03MHH153 pKa = 7.1ALCGNRR159 pKa = 11.84PGAPEE164 pKa = 3.92RR165 pKa = 11.84VRR167 pKa = 11.84GRR169 pKa = 11.84GPPLRR174 pKa = 11.84KK175 pKa = 9.42NRR177 pKa = 11.84PRR179 pKa = 11.84VADD182 pKa = 3.72RR183 pKa = 11.84RR184 pKa = 11.84PAVRR188 pKa = 11.84LPPLTRR194 pKa = 11.84EE195 pKa = 3.58GRR197 pKa = 11.84AAVDD201 pKa = 3.53LGFDD205 pKa = 4.95VDD207 pKa = 6.5DD208 pKa = 5.28LDD210 pKa = 3.88QDD212 pKa = 3.54AFEE215 pKa = 5.0FGDD218 pKa = 3.18VGGRR222 pKa = 11.84VGVVEE227 pKa = 4.95RR228 pKa = 11.84GAVIIDD234 pKa = 3.91PPRR237 pKa = 11.84APARR241 pKa = 11.84AFVFGDD247 pKa = 3.02LGEE250 pKa = 4.27PVEE253 pKa = 4.19VEE255 pKa = 3.6EE256 pKa = 5.06GGVVFVNPPRR266 pKa = 11.84CEE268 pKa = 3.91APALPVEE275 pKa = 4.98PIAAKK280 pKa = 10.35GPEE283 pKa = 4.34PEE285 pKa = 4.6VPICLPLPEE294 pKa = 5.69EE295 pKa = 4.2ILEE298 pKa = 4.36STDD301 pKa = 3.21LAAVQGPPLAWDD313 pKa = 3.68VSRR316 pKa = 11.84LVKK319 pKa = 10.03PAFLVPEE326 pKa = 4.44PPDD329 pKa = 3.57NKK331 pKa = 10.54GKK333 pKa = 9.51PRR335 pKa = 11.84DD336 pKa = 3.74LSWDD340 pKa = 3.39EE341 pKa = 3.7RR342 pKa = 11.84VMRR345 pKa = 11.84EE346 pKa = 3.94QKK348 pKa = 10.69LSALGTRR355 pKa = 11.84GSSHH359 pKa = 5.37TAYY362 pKa = 10.78FLGNQTLSMRR372 pKa = 11.84EE373 pKa = 3.5IYY375 pKa = 9.65KK376 pKa = 10.19RR377 pKa = 11.84KK378 pKa = 9.69HH379 pKa = 4.56NRR381 pKa = 11.84RR382 pKa = 11.84WWLWVYY388 pKa = 11.3LLTCYY393 pKa = 10.51LLCSFVFGFSIWLEE407 pKa = 4.32SLWGMVLVSAVVVSAFCLFIWHH429 pKa = 7.53WYY431 pKa = 7.85SVMWGIVTYY440 pKa = 9.45MVVLEE445 pKa = 4.6DD446 pKa = 3.64VPDD449 pKa = 4.21RR450 pKa = 11.84EE451 pKa = 4.54TEE453 pKa = 3.83HH454 pKa = 6.93RR455 pKa = 11.84RR456 pKa = 11.84LADD459 pKa = 3.36SEE461 pKa = 4.27QPDD464 pKa = 3.9IRR466 pKa = 11.84THH468 pKa = 6.4PLAKK472 pKa = 10.12GAIEE476 pKa = 4.1RR477 pKa = 11.84PVKK480 pKa = 9.82EE481 pKa = 3.24RR482 pKa = 11.84RR483 pKa = 11.84ARR485 pKa = 11.84VSRR488 pKa = 11.84VEE490 pKa = 3.84KK491 pKa = 10.6NFWGGEE497 pKa = 4.04FKK499 pKa = 10.62PCRR502 pKa = 11.84DD503 pKa = 2.93RR504 pKa = 11.84TLPVSQQLLDD514 pKa = 3.98DD515 pKa = 4.44CMSPRR520 pKa = 11.84IVPQSIMGDD529 pKa = 3.43DD530 pKa = 3.32AAVAEE535 pKa = 4.32RR536 pKa = 11.84VGRR539 pKa = 11.84YY540 pKa = 8.32LATCSHH546 pKa = 6.46VNTANSDD553 pKa = 3.5QLIQNTGEE561 pKa = 3.97IARR564 pKa = 11.84LLIAEE569 pKa = 5.01RR570 pKa = 11.84FNNWSVHH577 pKa = 5.49RR578 pKa = 11.84GQGGFLPGASS588 pKa = 3.4

Molecular weight: 65.2 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1L3KFX0|A0A1L3KFX0_9VIRU Uncharacterized protein OS=Changjiang tombus-like virus 22 OX=1922816 PE=4 SV=1
MM1 pKa = 7.31LFSVGCHH8 pKa = 5.33MDD10 pKa = 3.68GAALPTPDD18 pKa = 3.26ITSSHH23 pKa = 5.92TLLAGVAARR32 pKa = 11.84FATQPPRR39 pKa = 11.84PDD41 pKa = 3.37DD42 pKa = 3.56VLMGEE47 pKa = 4.81LEE49 pKa = 4.21QFVLRR54 pKa = 11.84WLEE57 pKa = 4.02EE58 pKa = 3.78NLTPLSADD66 pKa = 3.51CDD68 pKa = 3.86VSFDD72 pKa = 3.56SWVAGCNQPDD82 pKa = 3.19WRR84 pKa = 11.84KK85 pKa = 10.51AEE87 pKa = 3.9LRR89 pKa = 11.84EE90 pKa = 4.58AFDD93 pKa = 3.26SWVVPEE99 pKa = 4.74SKK101 pKa = 10.72HH102 pKa = 6.39FDD104 pKa = 2.98VKK106 pKa = 10.8MFMKK110 pKa = 10.43KK111 pKa = 9.74EE112 pKa = 4.14SYY114 pKa = 10.18TDD116 pKa = 3.27WKK118 pKa = 9.99HH119 pKa = 5.28GRR121 pKa = 11.84AINSRR126 pKa = 11.84TDD128 pKa = 2.87AFKK131 pKa = 10.87TMVGPFFSAIEE142 pKa = 3.98HH143 pKa = 5.82EE144 pKa = 4.74VFKK147 pKa = 10.95RR148 pKa = 11.84PEE150 pKa = 4.36FIKK153 pKa = 10.5KK154 pKa = 10.18VPVTDD159 pKa = 3.5RR160 pKa = 11.84ASYY163 pKa = 10.26VYY165 pKa = 10.31HH166 pKa = 6.8RR167 pKa = 11.84LYY169 pKa = 9.63RR170 pKa = 11.84TNGHH174 pKa = 6.19YY175 pKa = 10.39YY176 pKa = 10.72SSDD179 pKa = 3.3FTSFEE184 pKa = 3.97ALFTRR189 pKa = 11.84RR190 pKa = 11.84LMEE193 pKa = 3.83IVEE196 pKa = 4.35FSLYY200 pKa = 10.72RR201 pKa = 11.84HH202 pKa = 5.21MTKK205 pKa = 10.24HH206 pKa = 5.91LPSGGWFMGLISRR219 pKa = 11.84VLLGKK224 pKa = 10.24NKK226 pKa = 9.79IKK228 pKa = 10.2NRR230 pKa = 11.84HH231 pKa = 4.9MSLEE235 pKa = 4.13VEE237 pKa = 4.41ATRR240 pKa = 11.84MSGEE244 pKa = 4.07MCTSLGNGFSNLMFNLFVASKK265 pKa = 10.44SGVDD269 pKa = 2.89IDD271 pKa = 4.61GVVEE275 pKa = 4.22GDD277 pKa = 3.89DD278 pKa = 3.88GLFVTSGQIDD288 pKa = 3.54DD289 pKa = 4.49SLFEE293 pKa = 4.18KK294 pKa = 10.69LGLIIKK300 pKa = 9.77IEE302 pKa = 4.0RR303 pKa = 11.84HH304 pKa = 5.86EE305 pKa = 4.82DD306 pKa = 3.07PSTASFCGQIFDD318 pKa = 4.02PQNFVVITDD327 pKa = 3.59PRR329 pKa = 11.84KK330 pKa = 9.44VLASAGWIDD339 pKa = 4.42GKK341 pKa = 11.09YY342 pKa = 10.35LGAKK346 pKa = 9.61RR347 pKa = 11.84GKK349 pKa = 9.74QLGLLRR355 pKa = 11.84AKK357 pKa = 9.94AWSFGYY363 pKa = 10.04QYY365 pKa = 9.82PEE367 pKa = 4.23CPIVSAMARR376 pKa = 11.84ALLRR380 pKa = 11.84LTRR383 pKa = 11.84SMDD386 pKa = 2.92HH387 pKa = 6.25RR388 pKa = 11.84VVFKK392 pKa = 10.73TDD394 pKa = 3.37LGWWLRR400 pKa = 11.84KK401 pKa = 9.22EE402 pKa = 3.79YY403 pKa = 10.34QAAFDD408 pKa = 3.64HH409 pKa = 6.59GRR411 pKa = 11.84PEE413 pKa = 4.0LNRR416 pKa = 11.84PVRR419 pKa = 11.84DD420 pKa = 3.56EE421 pKa = 4.21TRR423 pKa = 11.84SLMHH427 pKa = 6.52KK428 pKa = 10.32VFGVSPDD435 pKa = 3.44TQRR438 pKa = 11.84EE439 pKa = 3.74IEE441 pKa = 3.95AWFDD445 pKa = 3.14NLQEE449 pKa = 4.11IRR451 pKa = 11.84PFPSLDD457 pKa = 3.4VPEE460 pKa = 4.18SWKK463 pKa = 10.71EE464 pKa = 3.74YY465 pKa = 7.62FQSYY469 pKa = 7.06VVRR472 pKa = 11.84TLNTDD477 pKa = 2.89WADD480 pKa = 3.9LPPEE484 pKa = 4.22SWNKK488 pKa = 10.23DD489 pKa = 3.41YY490 pKa = 11.48CVVLPAEE497 pKa = 4.95LDD499 pKa = 3.57PGG501 pKa = 3.84

Molecular weight: 57.35 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2

0

2

1089

501

588

544.5

61.28

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.346 ± 0.697

1.561 ± 0.099

6.887 ± 0.06

7.071 ± 0.411

4.867 ± 0.915

7.622 ± 0.743

2.296 ± 0.3

3.398 ± 0.118

3.581 ± 1.088

8.999 ± 0.01

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.479 ± 0.43

3.03 ± 0.142

6.979 ± 0.957

2.204 ± 0.115

7.805 ± 0.734

6.887 ± 0.42

3.489 ± 0.783

8.356 ± 0.705

2.755 ± 0.024

2.388 ± 0.245
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski