Amino acid dipepetide frequency for Streptomyces phage phiC31 (Bacteriophage phi-C31)

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
14.213AlaAla: 14.213 ± 1.18
1.205AlaCys: 1.205 ± 0.353
11.323AlaAsp: 11.323 ± 1.156
9.476AlaGlu: 9.476 ± 1.365
3.614AlaPhe: 3.614 ± 0.515
7.629AlaGly: 7.629 ± 0.847
2.73AlaHis: 2.73 ± 0.442
4.417AlaIle: 4.417 ± 0.563
4.979AlaLys: 4.979 ± 0.808
11.242AlaLeu: 11.242 ± 0.837
3.051AlaMet: 3.051 ± 0.569
3.373AlaAsn: 3.373 ± 0.539
4.898AlaPro: 4.898 ± 0.76
3.854AlaGln: 3.854 ± 0.469
7.629AlaArg: 7.629 ± 0.827
6.103AlaSer: 6.103 ± 0.623
8.11AlaThr: 8.11 ± 0.63
9.074AlaVal: 9.074 ± 0.843
2.57AlaTrp: 2.57 ± 0.434
4.015AlaTyr: 4.015 ± 0.533
0.0AlaXaa: 0.0 ± 0.0
Cys
1.445CysAla: 1.445 ± 0.41
0.0CysCys: 0.0 ± 0.0
0.883CysAsp: 0.883 ± 0.204
0.562CysGlu: 0.562 ± 0.177
0.08CysPhe: 0.08 ± 0.081
0.964CysGly: 0.964 ± 0.297
0.08CysHis: 0.08 ± 0.095
0.241CysIle: 0.241 ± 0.128
0.402CysLys: 0.402 ± 0.163
0.642CysLeu: 0.642 ± 0.254
0.08CysMet: 0.08 ± 0.067
0.402CysAsn: 0.402 ± 0.164
0.562CysPro: 0.562 ± 0.247
0.241CysGln: 0.241 ± 0.132
0.883CysArg: 0.883 ± 0.274
0.482CysSer: 0.482 ± 0.192
0.723CysThr: 0.723 ± 0.246
0.562CysVal: 0.562 ± 0.217
0.241CysTrp: 0.241 ± 0.143
0.402CysTyr: 0.402 ± 0.185
0.0CysXaa: 0.0 ± 0.0
Asp
10.6AspAla: 10.6 ± 1.534
0.642AspCys: 0.642 ± 0.274
4.818AspAsp: 4.818 ± 0.789
3.854AspGlu: 3.854 ± 0.629
2.168AspPhe: 2.168 ± 0.394
7.629AspGly: 7.629 ± 0.597
1.124AspHis: 1.124 ± 0.306
1.365AspIle: 1.365 ± 0.297
2.811AspLys: 2.811 ± 0.474
5.541AspLeu: 5.541 ± 0.784
1.365AspMet: 1.365 ± 0.318
1.365AspAsn: 1.365 ± 0.411
4.256AspPro: 4.256 ± 0.612
0.803AspGln: 0.803 ± 0.253
3.854AspArg: 3.854 ± 0.631
3.212AspSer: 3.212 ± 0.593
3.132AspThr: 3.132 ± 0.384
7.067AspVal: 7.067 ± 0.657
2.088AspTrp: 2.088 ± 0.384
1.445AspTyr: 1.445 ± 0.275
0.0AspXaa: 0.0 ± 0.0
Glu
8.753GluAla: 8.753 ± 0.914
0.642GluCys: 0.642 ± 0.221
3.614GluAsp: 3.614 ± 0.621
3.533GluGlu: 3.533 ± 0.611
2.008GluPhe: 2.008 ± 0.338
4.738GluGly: 4.738 ± 0.681
1.044GluHis: 1.044 ± 0.26
2.65GluIle: 2.65 ± 0.544
1.686GluLys: 1.686 ± 0.519
5.461GluLeu: 5.461 ± 1.011
0.803GluMet: 0.803 ± 0.26
1.044GluAsn: 1.044 ± 0.327
3.854GluPro: 3.854 ± 1.043
1.044GluGln: 1.044 ± 0.258
5.139GluArg: 5.139 ± 0.792
3.051GluSer: 3.051 ± 0.482
4.577GluThr: 4.577 ± 0.496
3.694GluVal: 3.694 ± 0.509
1.365GluTrp: 1.365 ± 0.304
1.847GluTyr: 1.847 ± 0.438
0.0GluXaa: 0.0 ± 0.0
Phe
3.854PheAla: 3.854 ± 0.731
0.08PheCys: 0.08 ± 0.083
2.489PheAsp: 2.489 ± 0.527
1.767PheGlu: 1.767 ± 0.416
0.642PhePhe: 0.642 ± 0.204
2.811PheGly: 2.811 ± 0.478
0.161PheHis: 0.161 ± 0.116
0.803PheIle: 0.803 ± 0.244
1.686PheLys: 1.686 ± 0.295
2.008PheLeu: 2.008 ± 0.444
1.124PheMet: 1.124 ± 0.366
1.606PheAsn: 1.606 ± 0.316
1.686PhePro: 1.686 ± 0.404
0.642PheGln: 0.642 ± 0.204
2.088PheArg: 2.088 ± 0.44
1.526PheSer: 1.526 ± 0.352
2.73PheThr: 2.73 ± 0.478
2.73PheVal: 2.73 ± 0.469
0.241PheTrp: 0.241 ± 0.126
0.562PheTyr: 0.562 ± 0.17
0.0PheXaa: 0.0 ± 0.0
Gly
10.52GlyAla: 10.52 ± 0.962
0.883GlyCys: 0.883 ± 0.256
5.3GlyAsp: 5.3 ± 0.684
4.898GlyGlu: 4.898 ± 0.738
3.694GlyPhe: 3.694 ± 0.568
6.344GlyGly: 6.344 ± 0.799
1.365GlyHis: 1.365 ± 0.304
3.614GlyIle: 3.614 ± 0.586
4.577GlyLys: 4.577 ± 0.852
7.147GlyLeu: 7.147 ± 1.249
1.526GlyMet: 1.526 ± 0.385
2.891GlyAsn: 2.891 ± 0.432
4.577GlyPro: 4.577 ± 0.952
2.409GlyGln: 2.409 ± 0.389
6.986GlyArg: 6.986 ± 0.553
4.658GlySer: 4.658 ± 0.671
4.898GlyThr: 4.898 ± 0.862
5.621GlyVal: 5.621 ± 0.854
2.088GlyTrp: 2.088 ± 0.401
3.614GlyTyr: 3.614 ± 0.807
0.0GlyXaa: 0.0 ± 0.0
His
2.088HisAla: 2.088 ± 0.445
0.321HisCys: 0.321 ± 0.179
1.445HisAsp: 1.445 ± 0.29
0.964HisGlu: 0.964 ± 0.305
0.803HisPhe: 0.803 ± 0.244
1.445HisGly: 1.445 ± 0.298
0.241HisHis: 0.241 ± 0.143
0.642HisIle: 0.642 ± 0.231
0.562HisLys: 0.562 ± 0.248
1.285HisLeu: 1.285 ± 0.311
0.241HisMet: 0.241 ± 0.14
0.562HisAsn: 0.562 ± 0.199
0.964HisPro: 0.964 ± 0.28
0.161HisGln: 0.161 ± 0.111
1.044HisArg: 1.044 ± 0.26
1.365HisSer: 1.365 ± 0.312
0.803HisThr: 0.803 ± 0.246
1.205HisVal: 1.205 ± 0.368
0.562HisTrp: 0.562 ± 0.242
0.482HisTyr: 0.482 ± 0.184
0.0HisXaa: 0.0 ± 0.0
Ile
3.854IleAla: 3.854 ± 0.602
0.241IleCys: 0.241 ± 0.155
3.051IleAsp: 3.051 ± 0.492
3.132IleGlu: 3.132 ± 0.524
1.044IlePhe: 1.044 ± 0.276
2.811IleGly: 2.811 ± 0.486
1.124IleHis: 1.124 ± 0.404
1.365IleIle: 1.365 ± 0.339
2.008IleLys: 2.008 ± 0.394
1.927IleLeu: 1.927 ± 0.405
0.803IleMet: 0.803 ± 0.198
0.642IleAsn: 0.642 ± 0.232
2.008IlePro: 2.008 ± 0.387
0.883IleGln: 0.883 ± 0.28
2.088IleArg: 2.088 ± 0.498
1.686IleSer: 1.686 ± 0.376
2.811IleThr: 2.811 ± 0.404
2.73IleVal: 2.73 ± 0.41
0.321IleTrp: 0.321 ± 0.135
0.723IleTyr: 0.723 ± 0.204
0.0IleXaa: 0.0 ± 0.0
Lys
5.3LysAla: 5.3 ± 0.905
0.402LysCys: 0.402 ± 0.216
2.489LysAsp: 2.489 ± 0.524
2.008LysGlu: 2.008 ± 0.429
1.445LysPhe: 1.445 ± 0.342
2.891LysGly: 2.891 ± 0.665
1.365LysHis: 1.365 ± 0.378
1.606LysIle: 1.606 ± 0.442
1.445LysLys: 1.445 ± 0.342
4.095LysLeu: 4.095 ± 0.745
0.642LysMet: 0.642 ± 0.218
1.365LysAsn: 1.365 ± 0.363
3.292LysPro: 3.292 ± 0.537
0.562LysGln: 0.562 ± 0.195
4.658LysArg: 4.658 ± 0.595
2.57LysSer: 2.57 ± 0.561
2.409LysThr: 2.409 ± 0.46
4.015LysVal: 4.015 ± 0.525
0.642LysTrp: 0.642 ± 0.22
1.044LysTyr: 1.044 ± 0.302
0.0LysXaa: 0.0 ± 0.0
Leu
8.271LeuAla: 8.271 ± 0.87
0.562LeuCys: 0.562 ± 0.161
6.585LeuAsp: 6.585 ± 0.788
3.373LeuGlu: 3.373 ± 0.59
2.008LeuPhe: 2.008 ± 0.385
5.621LeuGly: 5.621 ± 0.663
1.044LeuHis: 1.044 ± 0.261
3.614LeuIle: 3.614 ± 0.517
4.577LeuLys: 4.577 ± 0.711
4.095LeuLeu: 4.095 ± 0.691
0.642LeuMet: 0.642 ± 0.232
3.453LeuAsn: 3.453 ± 0.479
5.22LeuPro: 5.22 ± 0.696
2.73LeuGln: 2.73 ± 0.419
5.3LeuArg: 5.3 ± 0.896
5.461LeuSer: 5.461 ± 0.691
5.862LeuThr: 5.862 ± 0.85
5.22LeuVal: 5.22 ± 0.641
1.044LeuTrp: 1.044 ± 0.259
2.088LeuTyr: 2.088 ± 0.396
0.0LeuXaa: 0.0 ± 0.0
Met
3.132MetAla: 3.132 ± 0.512
0.321MetCys: 0.321 ± 0.142
1.124MetAsp: 1.124 ± 0.363
0.482MetGlu: 0.482 ± 0.164
0.241MetPhe: 0.241 ± 0.125
2.088MetGly: 2.088 ± 0.395
0.321MetHis: 0.321 ± 0.149
0.642MetIle: 0.642 ± 0.223
1.285MetLys: 1.285 ± 0.326
1.445MetLeu: 1.445 ± 0.274
0.161MetMet: 0.161 ± 0.116
0.321MetAsn: 0.321 ± 0.154
1.365MetPro: 1.365 ± 0.355
0.321MetGln: 0.321 ± 0.129
1.847MetArg: 1.847 ± 0.374
1.205MetSer: 1.205 ± 0.346
1.365MetThr: 1.365 ± 0.239
1.767MetVal: 1.767 ± 0.472
0.642MetTrp: 0.642 ± 0.206
0.241MetTyr: 0.241 ± 0.151
0.0MetXaa: 0.0 ± 0.0
Asn
5.3AsnAla: 5.3 ± 0.629
0.161AsnCys: 0.161 ± 0.12
0.964AsnAsp: 0.964 ± 0.295
1.285AsnGlu: 1.285 ± 0.301
0.803AsnPhe: 0.803 ± 0.275
3.774AsnGly: 3.774 ± 0.436
0.723AsnHis: 0.723 ± 0.205
0.723AsnIle: 0.723 ± 0.246
1.606AsnLys: 1.606 ± 0.32
1.686AsnLeu: 1.686 ± 0.304
0.723AsnMet: 0.723 ± 0.244
0.803AsnAsn: 0.803 ± 0.276
2.489AsnPro: 2.489 ± 0.477
0.482AsnGln: 0.482 ± 0.222
1.124AsnArg: 1.124 ± 0.31
1.445AsnSer: 1.445 ± 0.307
1.205AsnThr: 1.205 ± 0.304
2.489AsnVal: 2.489 ± 0.453
0.562AsnTrp: 0.562 ± 0.267
0.402AsnTyr: 0.402 ± 0.188
0.0AsnXaa: 0.0 ± 0.0
Pro
6.264ProAla: 6.264 ± 0.706
0.562ProCys: 0.562 ± 0.256
4.898ProAsp: 4.898 ± 0.632
5.3ProGlu: 5.3 ± 0.911
1.927ProPhe: 1.927 ± 0.373
5.541ProGly: 5.541 ± 0.756
0.562ProHis: 0.562 ± 0.206
1.445ProIle: 1.445 ± 0.387
3.051ProLys: 3.051 ± 0.462
2.971ProLeu: 2.971 ± 0.603
1.044ProMet: 1.044 ± 0.278
2.088ProAsn: 2.088 ± 0.408
1.044ProPro: 1.044 ± 0.318
1.124ProGln: 1.124 ± 0.552
1.767ProArg: 1.767 ± 0.357
3.453ProSer: 3.453 ± 0.541
4.738ProThr: 4.738 ± 0.628
4.256ProVal: 4.256 ± 0.655
0.883ProTrp: 0.883 ± 0.296
1.205ProTyr: 1.205 ± 0.3
0.0ProXaa: 0.0 ± 0.0
Gln
3.453GlnAla: 3.453 ± 0.442
0.241GlnCys: 0.241 ± 0.122
0.964GlnAsp: 0.964 ± 0.216
0.562GlnGlu: 0.562 ± 0.253
0.883GlnPhe: 0.883 ± 0.228
2.248GlnGly: 2.248 ± 0.407
0.482GlnHis: 0.482 ± 0.197
0.883GlnIle: 0.883 ± 0.211
0.482GlnLys: 0.482 ± 0.279
1.526GlnLeu: 1.526 ± 0.431
0.723GlnMet: 0.723 ± 0.219
0.803GlnAsn: 0.803 ± 0.281
1.285GlnPro: 1.285 ± 0.279
0.321GlnGln: 0.321 ± 0.183
3.614GlnArg: 3.614 ± 0.593
1.044GlnSer: 1.044 ± 0.253
1.445GlnThr: 1.445 ± 0.382
2.088GlnVal: 2.088 ± 0.442
0.402GlnTrp: 0.402 ± 0.175
0.642GlnTyr: 0.642 ± 0.208
0.0GlnXaa: 0.0 ± 0.0
Arg
8.191ArgAla: 8.191 ± 0.763
0.803ArgCys: 0.803 ± 0.346
4.979ArgAsp: 4.979 ± 0.76
4.417ArgGlu: 4.417 ± 0.575
2.65ArgPhe: 2.65 ± 0.513
5.701ArgGly: 5.701 ± 0.606
1.285ArgHis: 1.285 ± 0.341
3.292ArgIle: 3.292 ± 0.651
4.417ArgLys: 4.417 ± 0.554
4.658ArgLeu: 4.658 ± 0.57
1.847ArgMet: 1.847 ± 0.357
1.927ArgAsn: 1.927 ± 0.447
1.927ArgPro: 1.927 ± 0.35
1.686ArgGln: 1.686 ± 0.369
3.614ArgArg: 3.614 ± 0.563
3.935ArgSer: 3.935 ± 0.666
3.774ArgThr: 3.774 ± 0.619
5.461ArgVal: 5.461 ± 0.707
1.365ArgTrp: 1.365 ± 0.341
2.329ArgTyr: 2.329 ± 0.513
0.0ArgXaa: 0.0 ± 0.0
Ser
7.227SerAla: 7.227 ± 0.818
0.402SerCys: 0.402 ± 0.172
3.373SerAsp: 3.373 ± 0.573
3.453SerGlu: 3.453 ± 0.429
1.445SerPhe: 1.445 ± 0.337
5.701SerGly: 5.701 ± 0.762
0.803SerHis: 0.803 ± 0.275
2.168SerIle: 2.168 ± 0.346
2.088SerLys: 2.088 ± 0.415
4.738SerLeu: 4.738 ± 0.599
1.205SerMet: 1.205 ± 0.247
0.803SerAsn: 0.803 ± 0.311
2.891SerPro: 2.891 ± 0.572
1.205SerGln: 1.205 ± 0.25
3.051SerArg: 3.051 ± 0.458
2.811SerSer: 2.811 ± 0.557
4.658SerThr: 4.658 ± 0.692
4.336SerVal: 4.336 ± 0.683
0.964SerTrp: 0.964 ± 0.252
1.526SerTyr: 1.526 ± 0.422
0.0SerXaa: 0.0 ± 0.0
Thr
6.986ThrAla: 6.986 ± 0.685
0.803ThrCys: 0.803 ± 0.244
3.453ThrAsp: 3.453 ± 0.673
4.095ThrGlu: 4.095 ± 0.499
2.329ThrPhe: 2.329 ± 0.405
7.388ThrGly: 7.388 ± 0.98
0.883ThrHis: 0.883 ± 0.254
2.088ThrIle: 2.088 ± 0.34
2.891ThrLys: 2.891 ± 0.451
6.585ThrLeu: 6.585 ± 0.901
1.124ThrMet: 1.124 ± 0.273
1.847ThrAsn: 1.847 ± 0.454
4.738ThrPro: 4.738 ± 0.724
2.329ThrGln: 2.329 ± 0.419
2.811ThrArg: 2.811 ± 0.583
3.132ThrSer: 3.132 ± 0.454
4.577ThrThr: 4.577 ± 0.674
6.023ThrVal: 6.023 ± 0.896
1.445ThrTrp: 1.445 ± 0.359
2.168ThrTyr: 2.168 ± 0.515
0.0ThrXaa: 0.0 ± 0.0
Val
8.11ValAla: 8.11 ± 0.785
0.803ValCys: 0.803 ± 0.265
3.935ValAsp: 3.935 ± 0.594
4.658ValGlu: 4.658 ± 0.884
2.329ValPhe: 2.329 ± 0.469
7.227ValGly: 7.227 ± 0.945
1.124ValHis: 1.124 ± 0.305
3.051ValIle: 3.051 ± 0.457
2.409ValLys: 2.409 ± 0.519
6.344ValLeu: 6.344 ± 0.591
2.168ValMet: 2.168 ± 0.443
2.409ValAsn: 2.409 ± 0.48
5.461ValPro: 5.461 ± 0.748
1.686ValGln: 1.686 ± 0.354
5.942ValArg: 5.942 ± 0.748
4.898ValSer: 4.898 ± 0.684
6.906ValThr: 6.906 ± 0.851
6.023ValVal: 6.023 ± 0.697
1.606ValTrp: 1.606 ± 0.358
2.088ValTyr: 2.088 ± 0.401
0.0ValXaa: 0.0 ± 0.0
Trp
2.409TrpAla: 2.409 ± 0.348
0.241TrpCys: 0.241 ± 0.132
1.365TrpAsp: 1.365 ± 0.302
1.044TrpGlu: 1.044 ± 0.31
0.803TrpPhe: 0.803 ± 0.296
1.365TrpGly: 1.365 ± 0.266
0.321TrpHis: 0.321 ± 0.165
0.402TrpIle: 0.402 ± 0.168
0.321TrpLys: 0.321 ± 0.14
2.248TrpLeu: 2.248 ± 0.418
0.161TrpMet: 0.161 ± 0.1
0.161TrpAsn: 0.161 ± 0.117
0.321TrpPro: 0.321 ± 0.137
1.044TrpGln: 1.044 ± 0.23
2.008TrpArg: 2.008 ± 0.492
1.445TrpSer: 1.445 ± 0.353
1.285TrpThr: 1.285 ± 0.307
1.767TrpVal: 1.767 ± 0.46
0.321TrpTrp: 0.321 ± 0.226
0.562TrpTyr: 0.562 ± 0.219
0.0TrpXaa: 0.0 ± 0.0
Tyr
3.614TyrAla: 3.614 ± 0.55
0.562TyrCys: 0.562 ± 0.206
2.088TyrAsp: 2.088 ± 0.428
1.686TyrGlu: 1.686 ± 0.412
0.321TyrPhe: 0.321 ± 0.18
3.694TyrGly: 3.694 ± 0.586
0.402TyrHis: 0.402 ± 0.174
0.241TyrIle: 0.241 ± 0.139
0.964TyrLys: 0.964 ± 0.295
1.124TyrLeu: 1.124 ± 0.347
0.803TyrMet: 0.803 ± 0.283
0.883TyrAsn: 0.883 ± 0.223
1.285TyrPro: 1.285 ± 0.373
0.562TyrGln: 0.562 ± 0.194
2.811TyrArg: 2.811 ± 0.637
1.365TyrSer: 1.365 ± 0.31
1.686TyrThr: 1.686 ± 0.368
3.051TyrVal: 3.051 ± 0.606
0.241TyrTrp: 0.241 ± 0.156
0.402TyrTyr: 0.402 ± 0.185
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 53 proteins (12454 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski