Selenomonas sputigena (strain ATCC 35185 / DSM 20758 / VPI D19B-28)

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Firmicutes; Negativicutes; Selenomonadales; Selenomonadaceae; Selenomonas; Selenomonas sputigena

Average proteome isoelectric point is 6.2

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2243 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|F4EWI3|F4EWI3_SELS3 Dynamin family protein OS=Selenomonas sputigena (strain ATCC 35185 / DSM 20758 / VPI D19B-28) OX=546271 GN=Selsp_2214 PE=4 SV=1
MM1 pKa = 7.51NRR3 pKa = 11.84FCRR6 pKa = 11.84ILRR9 pKa = 11.84QAFAVLLMTFIIAGGGYY26 pKa = 10.33GEE28 pKa = 4.28AVKK31 pKa = 10.86AGWCFAASLDD41 pKa = 4.45LAGLEE46 pKa = 4.38DD47 pKa = 3.94RR48 pKa = 11.84AVDD51 pKa = 3.8IDD53 pKa = 4.05PSDD56 pKa = 3.8GSVSVTLSEE65 pKa = 4.95SEE67 pKa = 4.22MEE69 pKa = 4.21LLSSVRR75 pKa = 11.84CNLLYY80 pKa = 10.59LDD82 pKa = 4.35EE83 pKa = 4.72NVVIHH88 pKa = 6.76LGSDD92 pKa = 3.31ANIDD96 pKa = 3.88ADD98 pKa = 3.21WDD100 pKa = 3.89TGVFRR105 pKa = 11.84DD106 pKa = 4.38NFDD109 pKa = 3.67GTWPMLDD116 pKa = 3.2GHH118 pKa = 6.32PVYY121 pKa = 10.52IEE123 pKa = 3.88LTEE126 pKa = 4.54EE127 pKa = 4.38GDD129 pKa = 3.99DD130 pKa = 3.76YY131 pKa = 11.75NLYY134 pKa = 10.6SIPIKK139 pKa = 10.96LNGEE143 pKa = 3.91EE144 pKa = 5.07CILQVSYY151 pKa = 11.25NYY153 pKa = 10.57NDD155 pKa = 3.36EE156 pKa = 4.44TYY158 pKa = 10.2HH159 pKa = 7.63ILGAQRR165 pKa = 11.84EE166 pKa = 4.32PEE168 pKa = 4.01EE169 pKa = 5.34SGMSDD174 pKa = 2.86QGLIPLRR181 pKa = 11.84EE182 pKa = 4.04GDD184 pKa = 3.99MVTTIHH190 pKa = 6.27FAKK193 pKa = 10.4SVSDD197 pKa = 3.85NDD199 pKa = 3.9DD200 pKa = 4.01DD201 pKa = 4.1EE202 pKa = 4.83LAAVEE207 pKa = 5.19AEE209 pKa = 4.12TFQIGSHH216 pKa = 6.32PAVEE220 pKa = 4.47DD221 pKa = 3.82EE222 pKa = 4.21EE223 pKa = 6.35LGDD226 pKa = 3.59GTYY229 pKa = 10.9GYY231 pKa = 9.91IFEE234 pKa = 4.7FLAPTGEE241 pKa = 4.32SALSEE246 pKa = 3.58IAVFNIEE253 pKa = 4.34GGNITTFDD261 pKa = 3.56EE262 pKa = 4.51

Molecular weight: 28.77 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|C9LRM5|C9LRM5_SELS3 RNA polymerase sigma factor sigma-70 family OS=Selenomonas sputigena (strain ATCC 35185 / DSM 20758 / VPI D19B-28) OX=546271 GN=Selsp_1911 PE=4 SV=1
MM1 pKa = 7.22KK2 pKa = 10.11HH3 pKa = 5.81WDD5 pKa = 3.99LPEE8 pKa = 4.43DD9 pKa = 3.84WEE11 pKa = 4.7IEE13 pKa = 4.09AALPEE18 pKa = 4.36YY19 pKa = 11.13VDD21 pKa = 4.1GEE23 pKa = 4.38GDD25 pKa = 3.3ATRR28 pKa = 11.84LFLADD33 pKa = 3.15GTDD36 pKa = 2.97RR37 pKa = 11.84LVRR40 pKa = 11.84ARR42 pKa = 11.84LRR44 pKa = 11.84TVLEE48 pKa = 3.79RR49 pKa = 11.84LARR52 pKa = 11.84RR53 pKa = 11.84HH54 pKa = 5.9CRR56 pKa = 11.84SLPLLRR62 pKa = 11.84AWAKK66 pKa = 10.28EE67 pKa = 3.82RR68 pKa = 11.84TALAQTAPLAIASEE82 pKa = 4.28LVLVPFRR89 pKa = 11.84ARR91 pKa = 11.84RR92 pKa = 11.84PRR94 pKa = 11.84IRR96 pKa = 11.84GDD98 pKa = 2.87AAMGVVNAMHH108 pKa = 6.4AQLVRR113 pKa = 11.84AEE115 pKa = 4.23EE116 pKa = 3.9PAEE119 pKa = 4.1IEE121 pKa = 4.17LSCGRR126 pKa = 11.84RR127 pKa = 11.84IRR129 pKa = 11.84ALWGAATLAAHH140 pKa = 6.75LRR142 pKa = 11.84AAEE145 pKa = 4.32ILRR148 pKa = 11.84QDD150 pKa = 4.06LDD152 pKa = 3.8AKK154 pKa = 10.33TEE156 pKa = 3.98AAVLRR161 pKa = 11.84RR162 pKa = 11.84LFWQKK167 pKa = 9.79AWNRR171 pKa = 11.84AALGRR176 pKa = 11.84KK177 pKa = 8.43

Molecular weight: 20.03 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2243

0

2243

752766

32

4439

335.6

37.06

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

10.777 ± 0.081

1.133 ± 0.023

5.453 ± 0.04

7.513 ± 0.072

3.992 ± 0.045

7.69 ± 0.081

2.056 ± 0.021

5.811 ± 0.057

5.604 ± 0.044

9.721 ± 0.064

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.826 ± 0.029

3.128 ± 0.059

3.731 ± 0.033

2.977 ± 0.031

5.906 ± 0.057

5.311 ± 0.039

5.137 ± 0.081

7.14 ± 0.045

0.935 ± 0.022

3.16 ± 0.043
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski