Eubacterium sp. CAG:156

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Firmicutes; Clostridia; Eubacteriales; Eubacteriaceae; Eubacterium; environmental samples

Average proteome isoelectric point is 6.21

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2092 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|R5ZHB4|R5ZHB4_9FIRM Glycosyltransferases involved in cell wall biogenesis OS=Eubacterium sp. CAG:156 OX=1262880 GN=BN504_01846 PE=4 SV=1
MM1 pKa = 7.79RR2 pKa = 11.84KK3 pKa = 9.65KK4 pKa = 10.57FLLSVMLLVTMLTTLLFGNNVRR26 pKa = 11.84ADD28 pKa = 4.0YY29 pKa = 11.57YY30 pKa = 11.45DD31 pKa = 4.56DD32 pKa = 5.97FYY34 pKa = 11.81DD35 pKa = 3.79NEE37 pKa = 4.04YY38 pKa = 10.95DD39 pKa = 4.17YY40 pKa = 10.86EE41 pKa = 4.51YY42 pKa = 11.47SDD44 pKa = 5.7ADD46 pKa = 4.53DD47 pKa = 5.75LEE49 pKa = 5.63IDD51 pKa = 4.08YY52 pKa = 11.17KK53 pKa = 11.15SLSLNEE59 pKa = 4.17GSTQKK64 pKa = 10.15IEE66 pKa = 4.01YY67 pKa = 9.76VFVGDD72 pKa = 4.61AIVWRR77 pKa = 11.84SS78 pKa = 2.95

Molecular weight: 9.23 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|R5Z2P0|R5Z2P0_9FIRM t(6)A37 threonylcarbamoyladenosine biosynthesis protein TsaE OS=Eubacterium sp. CAG:156 OX=1262880 GN=BN504_01810 PE=3 SV=1
MM1 pKa = 7.59HH2 pKa = 7.51KK3 pKa = 10.93GKK5 pKa = 8.1MTYY8 pKa = 8.58QPKK11 pKa = 9.73KK12 pKa = 7.54RR13 pKa = 11.84QRR15 pKa = 11.84SKK17 pKa = 9.0VHH19 pKa = 5.93GFRR22 pKa = 11.84SRR24 pKa = 11.84MSTAGGRR31 pKa = 11.84KK32 pKa = 8.71VLAARR37 pKa = 11.84RR38 pKa = 11.84AKK40 pKa = 9.71GRR42 pKa = 11.84KK43 pKa = 8.73VLSAA47 pKa = 4.05

Molecular weight: 5.34 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2092

0

2092

690534

29

2402

330.1

37.16

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.434 ± 0.053

1.334 ± 0.023

6.009 ± 0.041

7.414 ± 0.071

4.018 ± 0.039

6.619 ± 0.052

1.381 ± 0.019

8.685 ± 0.053

8.762 ± 0.054

7.709 ± 0.049

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.985 ± 0.026

6.017 ± 0.052

2.729 ± 0.024

2.618 ± 0.025

3.306 ± 0.036

5.945 ± 0.049

5.665 ± 0.054

7.036 ± 0.044

0.789 ± 0.018

4.542 ± 0.046
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski