Neotoma lepida (Desert woodrat)

Taxonomy: cellular organisms; Eukaryota; Opisthokonta; Metazoa; Eumetazoa; Bilateria; Deuterostomia; Chordata; Craniata; Vertebrata; Gnathostomata; Teleostomi; Euteleostomi; Sarcopterygii; Dipnotetrapodomorpha; Tetrapoda; Amniota; Mammalia; Theria; Eutheria; Boreoeutheria; Euarchontoglires; Glires; Rodentia;

Average proteome isoelectric point is 6.65

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 24274 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1A6GBA2|A0A1A6GBA2_NEOLE Homeobox domain-containing protein OS=Neotoma lepida OX=56216 GN=A6R68_07926 PE=4 SV=1
MM1 pKa = 6.81EE2 pKa = 5.0TDD4 pKa = 3.42MPFSRR9 pKa = 11.84GGQRR13 pKa = 11.84NIEE16 pKa = 4.16KK17 pKa = 9.71AQSVSYY23 pKa = 11.18DD24 pKa = 3.79DD25 pKa = 5.52GDD27 pKa = 4.41GNNGDD32 pKa = 5.05DD33 pKa = 4.48NDD35 pKa = 4.45SGHH38 pKa = 7.33DD39 pKa = 3.71GGGGDD44 pKa = 5.74DD45 pKa = 5.3DD46 pKa = 6.58DD47 pKa = 6.36DD48 pKa = 4.41GHH50 pKa = 6.22GHH52 pKa = 6.52NDD54 pKa = 3.3GGGGGGNDD62 pKa = 3.62SNGSDD67 pKa = 4.01VLVMMMVMVVVVVVVVVMMVVMVVVVALVVMMVVMMMMVVVVMMVMMINIDD118 pKa = 3.16ITNFSSSWSDD128 pKa = 3.38GLALCALLHH137 pKa = 6.47TYY139 pKa = 10.54LPAHH143 pKa = 7.07IPYY146 pKa = 9.89QEE148 pKa = 4.62LNSQEE153 pKa = 4.46KK154 pKa = 9.76EE155 pKa = 4.06LSEE158 pKa = 3.93MLYY161 pKa = 10.04TDD163 pKa = 3.56RR164 pKa = 11.84PDD166 pKa = 3.22WQSVMQYY173 pKa = 9.62VAQIYY178 pKa = 10.26KK179 pKa = 10.45YY180 pKa = 10.83FEE182 pKa = 4.02TT183 pKa = 4.55

Molecular weight: 19.99 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1A6GUR6|A0A1A6GUR6_NEOLE Uncharacterized protein (Fragment) OS=Neotoma lepida OX=56216 GN=A6R68_01785 PE=4 SV=1
MM1 pKa = 7.57SSHH4 pKa = 5.15KK5 pKa = 8.91TFRR8 pKa = 11.84IKK10 pKa = 10.64RR11 pKa = 11.84FLAKK15 pKa = 9.71KK16 pKa = 9.58QKK18 pKa = 8.69QNRR21 pKa = 11.84PIPQWIRR28 pKa = 11.84MKK30 pKa = 9.89TGNKK34 pKa = 8.61IRR36 pKa = 11.84YY37 pKa = 7.09NSKK40 pKa = 8.3RR41 pKa = 11.84RR42 pKa = 11.84HH43 pKa = 3.95WRR45 pKa = 11.84RR46 pKa = 11.84TKK48 pKa = 10.83LGLL51 pKa = 3.67

Molecular weight: 6.41 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

24274

0

24274

8997059

8

6798

370.6

41.22

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.009 ± 0.019

2.213 ± 0.013

4.804 ± 0.012

6.914 ± 0.023

3.651 ± 0.013

6.461 ± 0.018

2.62 ± 0.008

4.281 ± 0.016

5.534 ± 0.019

10.162 ± 0.019

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.243 ± 0.007

3.456 ± 0.013

6.253 ± 0.023

4.785 ± 0.015

5.592 ± 0.016

8.358 ± 0.021

5.384 ± 0.013

6.206 ± 0.017

1.244 ± 0.007

2.664 ± 0.01
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski