Cassava satellite virus

Taxonomy: Viruses; Satellites; RNA satellites

Average proteome isoelectric point is 7.25

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1S5XW17|A0A1S5XW17_9VIRU ORF2 OS=Cassava satellite virus OX=1958957 PE=4 SV=1
MM1 pKa = 7.81KK2 pKa = 10.27EE3 pKa = 3.49ITFFLATLDD12 pKa = 4.12IPDD15 pKa = 3.82EE16 pKa = 4.48VYY18 pKa = 11.16DD19 pKa = 5.29FIMAHH24 pKa = 4.85YY25 pKa = 9.9VSWGWYY31 pKa = 9.93AGGKK35 pKa = 8.54CCEE38 pKa = 3.86VTFNFKK44 pKa = 10.87SEE46 pKa = 3.43VRR48 pKa = 11.84AFVNKK53 pKa = 10.43YY54 pKa = 9.2PDD56 pKa = 3.53PQIASGIYY64 pKa = 8.26TYY66 pKa = 11.21LLKK69 pKa = 10.5FYY71 pKa = 9.33YY72 pKa = 10.43LPIGVGRR79 pKa = 11.84LNIQNFDD86 pKa = 3.62LTRR89 pKa = 11.84SS90 pKa = 3.55

Molecular weight: 10.51 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1S5XW17|A0A1S5XW17_9VIRU ORF2 OS=Cassava satellite virus OX=1958957 PE=4 SV=1
MM1 pKa = 7.16GRR3 pKa = 11.84SRR5 pKa = 11.84NRR7 pKa = 11.84GRR9 pKa = 11.84RR10 pKa = 11.84RR11 pKa = 11.84RR12 pKa = 11.84VRR14 pKa = 11.84RR15 pKa = 11.84FRR17 pKa = 11.84PRR19 pKa = 11.84VPALTTLNYY28 pKa = 6.64TTASAGSQTTLARR41 pKa = 11.84KK42 pKa = 9.62DD43 pKa = 3.66FQVLAGCGDD52 pKa = 3.62RR53 pKa = 11.84SFKK56 pKa = 10.63IVGLKK61 pKa = 9.1LTVAALSEE69 pKa = 3.91PVIIQVHH76 pKa = 4.94VFNPGGQEE84 pKa = 3.19IGLNNTLIADD94 pKa = 3.67TRR96 pKa = 11.84TNLNLRR102 pKa = 11.84IPSSFKK108 pKa = 10.29EE109 pKa = 3.73WWQGEE114 pKa = 4.5TALSTTLVRR123 pKa = 11.84VDD125 pKa = 4.64LLRR128 pKa = 11.84TYY130 pKa = 10.72DD131 pKa = 3.59GQKK134 pKa = 10.91AKK136 pKa = 9.41VTFLLSCLVRR146 pKa = 11.84LGSPEE151 pKa = 3.75LTGRR155 pKa = 11.84SS156 pKa = 3.54

Molecular weight: 17.34 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2

0

2

246

90

156

123.0

13.93

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.098 ± 0.324

1.626 ± 0.356

4.065 ± 0.89

3.659 ± 0.469

5.691 ± 1.909

7.724 ± 0.631

0.813 ± 0.178

5.285 ± 1.488

4.472 ± 0.647

10.976 ± 1.909

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.22 ± 0.599

4.472 ± 0.016

4.065 ± 0.226

3.252 ± 0.615

8.943 ± 3.348

6.098 ± 0.987

8.537 ± 1.779

7.317 ± 0.388

1.626 ± 0.356

4.065 ± 2.879
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski