Brevibacillus phage Powder

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Myoviridae; Jimmervirus; Brevibacillus virus Osiris; Brevibacillus phage Osiris

Average proteome isoelectric point is 6.46

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 103 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A0K2FM89|A0A0K2FM89_9CAUD Uncharacterized protein OS=Brevibacillus phage Powder OX=1691957 GN=POWDER_23 PE=4 SV=1
MM1 pKa = 7.6DD2 pKa = 3.85TSLYY6 pKa = 11.08AEE8 pKa = 5.4FIGKK12 pKa = 8.82EE13 pKa = 3.83NPDD16 pKa = 3.93YY17 pKa = 11.2IKK19 pKa = 10.93DD20 pKa = 3.79SNTDD24 pKa = 3.49LNINFWLSTEE34 pKa = 5.11LYY36 pKa = 8.46PKK38 pKa = 10.29LISIPKK44 pKa = 9.09DD45 pKa = 3.12YY46 pKa = 11.29HH47 pKa = 6.47NILFAEE53 pKa = 4.2EE54 pKa = 4.26LTPKK58 pKa = 10.57LLQLLNIEE66 pKa = 4.05VDD68 pKa = 3.68SYY70 pKa = 11.52LVSSFVGEE78 pKa = 4.11PSS80 pKa = 2.93

Molecular weight: 9.22 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A0K2FLS5|A0A0K2FLS5_9CAUD Uncharacterized protein OS=Brevibacillus phage Powder OX=1691957 GN=POWDER_81 PE=4 SV=1
MM1 pKa = 7.54GMFDD5 pKa = 4.15EE6 pKa = 4.74VRR8 pKa = 11.84AVQKK12 pKa = 10.8PNFKK16 pKa = 10.44RR17 pKa = 11.84RR18 pKa = 11.84TKK20 pKa = 10.58KK21 pKa = 9.68RR22 pKa = 11.84GARR25 pKa = 11.84GRR27 pKa = 11.84ISPAVYY33 pKa = 10.34DD34 pKa = 3.76EE35 pKa = 4.24VMKK38 pKa = 10.46RR39 pKa = 11.84DD40 pKa = 3.22QGRR43 pKa = 11.84CVLCGKK49 pKa = 6.47TTWLQAHH56 pKa = 6.85HH57 pKa = 6.91IIFRR61 pKa = 11.84SEE63 pKa = 3.76GGTGEE68 pKa = 4.05AHH70 pKa = 6.77NLALACGPSTQTGTCHH86 pKa = 6.13WKK88 pKa = 9.77AHH90 pKa = 4.95HH91 pKa = 6.05TKK93 pKa = 10.26EE94 pKa = 3.95GRR96 pKa = 11.84QAFRR100 pKa = 11.84DD101 pKa = 3.76YY102 pKa = 10.94RR103 pKa = 11.84EE104 pKa = 4.16KK105 pKa = 11.2VLLPLYY111 pKa = 10.38RR112 pKa = 11.84GASS115 pKa = 3.44

Molecular weight: 13.05 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

103

0

103

16030

27

677

155.6

17.79

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

5.901 ± 0.244

0.873 ± 0.112

6.064 ± 0.223

8.21 ± 0.335

4.105 ± 0.204

6.064 ± 0.25

1.566 ± 0.099

7.405 ± 0.207

8.565 ± 0.266

8.21 ± 0.246

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.695 ± 0.15

5.028 ± 0.249

3.007 ± 0.182

3.974 ± 0.219

4.766 ± 0.257

5.976 ± 0.21

5.77 ± 0.207

6.438 ± 0.225

1.254 ± 0.097

4.13 ± 0.208
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski