Cyanobacteria bacterium PMG_004

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Cyanobacteria/Melainabacteria group; Cyanobacteria; unclassified Cyanobacteria

Average proteome isoelectric point is 7.7

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 142 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A4Q5PYA8|A0A4Q5PYA8_9CYAN Uncharacterized protein OS=Cyanobacteria bacterium PMG_004 OX=2773169 GN=EOO34_00225 PE=4 SV=1
MM1 pKa = 7.39EE2 pKa = 5.17SSAFFYY8 pKa = 10.35TIFLWCLLLSITGYY22 pKa = 10.1SIYY25 pKa = 10.9VGFGPPSKK33 pKa = 10.22EE34 pKa = 3.63LRR36 pKa = 11.84DD37 pKa = 3.63PFEE40 pKa = 4.47DD41 pKa = 4.9HH42 pKa = 7.31EE43 pKa = 4.53DD44 pKa = 3.35

Molecular weight: 5.12 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A4Q5Q0M3|A0A4Q5Q0M3_9CYAN ATP synthase subunit a OS=Cyanobacteria bacterium PMG_004 OX=2773169 GN=atpB PE=3 SV=1
MM1 pKa = 7.58AKK3 pKa = 10.21KK4 pKa = 10.83SMIEE8 pKa = 3.99RR9 pKa = 11.84EE10 pKa = 4.25KK11 pKa = 10.75KK12 pKa = 10.17RR13 pKa = 11.84QFLVIKK19 pKa = 9.16YY20 pKa = 8.55AKK22 pKa = 9.63KK23 pKa = 9.93RR24 pKa = 11.84NSLKK28 pKa = 10.91EE29 pKa = 4.08EE30 pKa = 3.76IKK32 pKa = 9.52KK33 pKa = 9.2TSFLEE38 pKa = 4.03EE39 pKa = 4.02KK40 pKa = 10.9LNIHH44 pKa = 6.92RR45 pKa = 11.84KK46 pKa = 7.56LQRR49 pKa = 11.84LPRR52 pKa = 11.84NSAPVRR58 pKa = 11.84LHH60 pKa = 5.48NRR62 pKa = 11.84CLITGRR68 pKa = 11.84PKK70 pKa = 10.56GYY72 pKa = 10.29YY73 pKa = 9.33RR74 pKa = 11.84DD75 pKa = 4.13FGLSRR80 pKa = 11.84HH81 pKa = 6.11LLRR84 pKa = 11.84EE85 pKa = 3.84MAHH88 pKa = 5.9NCLLPGVTKK97 pKa = 10.77SSWW100 pKa = 2.87

Molecular weight: 11.95 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

142

0

142

28495

21

2172

200.7

22.71

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

5.411 ± 0.312

1.263 ± 0.122

4.155 ± 0.184

5.12 ± 0.193

6.271 ± 0.264

5.864 ± 0.273

1.523 ± 0.114

6.966 ± 0.171

7.914 ± 0.503

10.956 ± 0.246

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.158 ± 0.158

5.236 ± 0.21

4.162 ± 0.123

4.071 ± 0.205

4.51 ± 0.229

7.787 ± 0.309

5.917 ± 0.193

6.506 ± 0.259

1.056 ± 0.11

3.151 ± 0.188
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski