Amino acid dipepetide frequency for Kribbella antibiotica

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10^-3.

For more information see sequence space article on Wikipedia.

	Ala	Cys	Asp	Glu	Phe	Gly	His	Ile	Lys	Leu	Met	Asn	Pro	Gln	Arg	Ser	Thr	Val	Trp	Tyr	Xaa
Ala	18.705AlaAla: 18.705 ± 0.141	0.85AlaCys: 0.85 ± 0.019	8.054AlaAsp: 8.054 ± 0.071	7.804AlaGlu: 7.804 ± 0.068	3.603AlaPhe: 3.603 ± 0.037	11.981AlaGly: 11.981 ± 0.065	2.187AlaHis: 2.187 ± 0.032	5.042AlaIle: 5.042 ± 0.044	3.496AlaLys: 3.496 ± 0.048	12.667AlaLeu: 12.667 ± 0.104	2.309AlaMet: 2.309 ± 0.03	2.703AlaAsn: 2.703 ± 0.036	5.758AlaPro: 5.758 ± 0.062	3.874AlaGln: 3.874 ± 0.044	7.745AlaArg: 7.745 ± 0.062	6.089AlaSer: 6.089 ± 0.046	7.638AlaThr: 7.638 ± 0.056	11.336AlaVal: 11.336 ± 0.075	1.866AlaTrp: 1.866 ± 0.026	2.602AlaTyr: 2.602 ± 0.032	0.0AlaXaa: 0.0 ± 0.0
Cys	0.791CysAla: 0.791 ± 0.018	0.084CysCys: 0.084 ± 0.005	0.408CysAsp: 0.408 ± 0.013	0.347CysGlu: 0.347 ± 0.011	0.222CysPhe: 0.222 ± 0.009	0.806CysGly: 0.806 ± 0.018	0.175CysHis: 0.175 ± 0.008	0.214CysIle: 0.214 ± 0.009	0.134CysLys: 0.134 ± 0.007	0.673CysLeu: 0.673 ± 0.016	0.115CysMet: 0.115 ± 0.007	0.186CysAsn: 0.186 ± 0.009	0.403CysPro: 0.403 ± 0.013	0.2CysGln: 0.2 ± 0.01	0.465CysArg: 0.465 ± 0.013	0.451CysSer: 0.451 ± 0.012	0.473CysThr: 0.473 ± 0.016	0.536CysVal: 0.536 ± 0.015	0.119CysTrp: 0.119 ± 0.007	0.162CysTyr: 0.162 ± 0.009	0.0CysXaa: 0.0 ± 0.0
Asp	6.978AspAla: 6.978 ± 0.053	0.394AspCys: 0.394 ± 0.013	3.764AspAsp: 3.764 ± 0.036	3.814AspGlu: 3.814 ± 0.04	1.709AspPhe: 1.709 ± 0.028	6.091AspGly: 6.091 ± 0.061	1.411AspHis: 1.411 ± 0.022	2.062AspIle: 2.062 ± 0.029	1.472AspLys: 1.472 ± 0.029	6.736AspLeu: 6.736 ± 0.056	0.69AspMet: 0.69 ± 0.015	1.312AspAsn: 1.312 ± 0.024	4.264AspPro: 4.264 ± 0.047	2.099AspGln: 2.099 ± 0.024	4.425AspArg: 4.425 ± 0.04	2.802AspSer: 2.802 ± 0.039	2.811AspThr: 2.811 ± 0.035	4.951AspVal: 4.951 ± 0.051	1.077AspTrp: 1.077 ± 0.024	1.293AspTyr: 1.293 ± 0.026	0.0AspXaa: 0.0 ± 0.0
Glu	6.225GluAla: 6.225 ± 0.066	0.305GluCys: 0.305 ± 0.011	2.384GluAsp: 2.384 ± 0.034	2.49GluGlu: 2.49 ± 0.037	1.634GluPhe: 1.634 ± 0.024	3.359GluGly: 3.359 ± 0.04	1.364GluHis: 1.364 ± 0.024	2.464GluIle: 2.464 ± 0.034	1.322GluLys: 1.322 ± 0.027	7.224GluLeu: 7.224 ± 0.071	0.852GluMet: 0.852 ± 0.02	1.081GluAsn: 1.081 ± 0.022	3.025GluPro: 3.025 ± 0.039	2.334GluGln: 2.334 ± 0.03	4.394GluArg: 4.394 ± 0.048	2.766GluSer: 2.766 ± 0.04	2.878GluThr: 2.878 ± 0.037	4.531GluVal: 4.531 ± 0.038	0.856GluTrp: 0.856 ± 0.018	1.224GluTyr: 1.224 ± 0.024	0.0GluXaa: 0.0 ± 0.0
Phe	3.792PheAla: 3.792 ± 0.039	0.255PheCys: 0.255 ± 0.009	2.194PheAsp: 2.194 ± 0.028	1.629PheGlu: 1.629 ± 0.027	0.954PhePhe: 0.954 ± 0.02	3.231PheGly: 3.231 ± 0.037	0.632PheHis: 0.632 ± 0.016	0.938PheIle: 0.938 ± 0.017	0.671PheLys: 0.671 ± 0.017	2.621PheLeu: 2.621 ± 0.038	0.397PheMet: 0.397 ± 0.012	0.747PheAsn: 0.747 ± 0.019	1.408PhePro: 1.408 ± 0.025	0.845PheGln: 0.845 ± 0.017	1.852PheArg: 1.852 ± 0.028	1.664PheSer: 1.664 ± 0.025	2.17PheThr: 2.17 ± 0.028	2.611PheVal: 2.611 ± 0.033	0.487PheTrp: 0.487 ± 0.014	0.704PheTyr: 0.704 ± 0.017	0.0PheXaa: 0.0 ± 0.0
Gly	9.413GlyAla: 9.413 ± 0.068	0.76GlyCys: 0.76 ± 0.018	4.886GlyAsp: 4.886 ± 0.048	4.271GlyGlu: 4.271 ± 0.044	3.034GlyPhe: 3.034 ± 0.037	7.549GlyGly: 7.549 ± 0.078	1.945GlyHis: 1.945 ± 0.025	3.98GlyIle: 3.98 ± 0.039	2.878GlyLys: 2.878 ± 0.044	9.251GlyLeu: 9.251 ± 0.073	1.701GlyMet: 1.701 ± 0.028	2.274GlyAsn: 2.274 ± 0.042	4.43GlyPro: 4.43 ± 0.037	2.957GlyGln: 2.957 ± 0.038	6.161GlyArg: 6.161 ± 0.052	5.409GlySer: 5.409 ± 0.046	5.996GlyThr: 5.996 ± 0.061	7.441GlyVal: 7.441 ± 0.065	1.826GlyTrp: 1.826 ± 0.028	2.481GlyTyr: 2.481 ± 0.034	0.0GlyXaa: 0.0 ± 0.0
His	2.209HisAla: 2.209 ± 0.031	0.177HisCys: 0.177 ± 0.009	1.235HisAsp: 1.235 ± 0.024	1.14HisGlu: 1.14 ± 0.024	0.628HisPhe: 0.628 ± 0.014	1.969HisGly: 1.969 ± 0.025	0.625HisHis: 0.625 ± 0.016	0.635HisIle: 0.635 ± 0.016	0.415HisLys: 0.415 ± 0.014	2.321HisLeu: 2.321 ± 0.034	0.272HisMet: 0.272 ± 0.009	0.473HisAsn: 0.473 ± 0.015	1.501HisPro: 1.501 ± 0.027	0.737HisGln: 0.737 ± 0.018	1.687HisArg: 1.687 ± 0.031	1.039HisSer: 1.039 ± 0.022	1.121HisThr: 1.121 ± 0.021	1.55HisVal: 1.55 ± 0.024	0.381HisTrp: 0.381 ± 0.013	0.495HisTyr: 0.495 ± 0.014	0.0HisXaa: 0.0 ± 0.0
Ile	5.834IleAla: 5.834 ± 0.045	0.327IleCys: 0.327 ± 0.011	2.979IleAsp: 2.979 ± 0.028	2.415IleGlu: 2.415 ± 0.03	1.031IlePhe: 1.031 ± 0.024	4.301IleGly: 4.301 ± 0.043	0.75IleHis: 0.75 ± 0.017	1.258IleIle: 1.258 ± 0.024	0.998IleLys: 0.998 ± 0.022	3.107IleLeu: 3.107 ± 0.038	0.493IleMet: 0.493 ± 0.015	0.965IleAsn: 0.965 ± 0.02	2.129IlePro: 2.129 ± 0.027	1.087IleGln: 1.087 ± 0.021	2.648IleArg: 2.648 ± 0.032	2.297IleSer: 2.297 ± 0.031	2.865IleThr: 2.865 ± 0.032	3.394IleVal: 3.394 ± 0.042	0.525IleTrp: 0.525 ± 0.014	0.806IleTyr: 0.806 ± 0.015	0.0IleXaa: 0.0 ± 0.0
Lys	3.389LysAla: 3.389 ± 0.042	0.126LysCys: 0.126 ± 0.007	1.371LysAsp: 1.371 ± 0.027	1.061LysGlu: 1.061 ± 0.02	0.705LysPhe: 0.705 ± 0.021	1.757LysGly: 1.757 ± 0.028	0.556LysHis: 0.556 ± 0.014	1.137LysIle: 1.137 ± 0.025	0.861LysLys: 0.861 ± 0.032	2.822LysLeu: 2.822 ± 0.036	0.43LysMet: 0.43 ± 0.012	0.702LysAsn: 0.702 ± 0.021	1.831LysPro: 1.831 ± 0.032	0.921LysGln: 0.921 ± 0.02	1.546LysArg: 1.546 ± 0.024	1.526LysSer: 1.526 ± 0.03	1.744LysThr: 1.744 ± 0.034	2.4LysVal: 2.4 ± 0.038	0.375LysTrp: 0.375 ± 0.012	0.685LysTyr: 0.685 ± 0.02	0.0LysXaa: 0.0 ± 0.0
Leu	14.293LeuAla: 14.293 ± 0.107	0.692LeuCys: 0.692 ± 0.018	6.543LeuAsp: 6.543 ± 0.057	4.912LeuGlu: 4.912 ± 0.05	2.749LeuPhe: 2.749 ± 0.033	8.885LeuGly: 8.885 ± 0.065	2.091LeuHis: 2.091 ± 0.032	4.303LeuIle: 4.303 ± 0.048	2.436LeuLys: 2.436 ± 0.036	10.595LeuLeu: 10.595 ± 0.098	1.67LeuMet: 1.67 ± 0.022	2.281LeuAsn: 2.281 ± 0.029	6.055LeuPro: 6.055 ± 0.059	3.035LeuGln: 3.035 ± 0.035	7.544LeuArg: 7.544 ± 0.069	5.846LeuSer: 5.846 ± 0.043	7.334LeuThr: 7.334 ± 0.057	8.859LeuVal: 8.859 ± 0.07	1.323LeuTrp: 1.323 ± 0.023	1.953LeuTyr: 1.953 ± 0.029	0.0LeuXaa: 0.0 ± 0.0
Met	2.023MetAla: 2.023 ± 0.03	0.098MetCys: 0.098 ± 0.006	0.813MetAsp: 0.813 ± 0.018	0.686MetGlu: 0.686 ± 0.016	0.518MetPhe: 0.518 ± 0.012	1.092MetGly: 1.092 ± 0.02	0.344MetHis: 0.344 ± 0.011	0.774MetIle: 0.774 ± 0.015	0.497MetLys: 0.497 ± 0.015	1.772MetLeu: 1.772 ± 0.029	0.299MetMet: 0.299 ± 0.011	0.472MetAsn: 0.472 ± 0.014	1.054MetPro: 1.054 ± 0.018	0.485MetGln: 0.485 ± 0.013	1.261MetArg: 1.261 ± 0.022	1.294MetSer: 1.294 ± 0.022	1.556MetThr: 1.556 ± 0.021	1.348MetVal: 1.348 ± 0.023	0.209MetTrp: 0.209 ± 0.007	0.32MetTyr: 0.32 ± 0.013	0.0MetXaa: 0.0 ± 0.0
Asn	2.686AsnAla: 2.686 ± 0.035	0.2AsnCys: 0.2 ± 0.008	1.354AsnAsp: 1.354 ± 0.03	1.076AsnGlu: 1.076 ± 0.021	0.717AsnPhe: 0.717 ± 0.014	2.526AsnGly: 2.526 ± 0.045	0.528AsnHis: 0.528 ± 0.013	0.821AsnIle: 0.821 ± 0.018	0.593AsnLys: 0.593 ± 0.017	2.306AsnLeu: 2.306 ± 0.028	0.34AsnMet: 0.34 ± 0.011	0.716AsnAsn: 0.716 ± 0.022	1.73AsnPro: 1.73 ± 0.025	0.825AsnGln: 0.825 ± 0.02	1.576AsnArg: 1.576 ± 0.024	1.244AsnSer: 1.244 ± 0.024	1.455AsnThr: 1.455 ± 0.027	1.733AsnVal: 1.733 ± 0.03	0.452AsnTrp: 0.452 ± 0.014	0.647AsnTyr: 0.647 ± 0.013	0.0AsnXaa: 0.0 ± 0.0
Pro	7.644ProAla: 7.644 ± 0.065	0.293ProCys: 0.293 ± 0.012	4.183ProAsp: 4.183 ± 0.047	3.71ProGlu: 3.71 ± 0.04	1.532ProPhe: 1.532 ± 0.024	5.565ProGly: 5.565 ± 0.053	1.094ProHis: 1.094 ± 0.022	2.053ProIle: 2.053 ± 0.03	1.459ProLys: 1.459 ± 0.025	4.846ProLeu: 4.846 ± 0.044	0.976ProMet: 0.976 ± 0.02	1.409ProAsn: 1.409 ± 0.022	2.965ProPro: 2.965 ± 0.046	1.719ProGln: 1.719 ± 0.026	3.058ProArg: 3.058 ± 0.038	3.257ProSer: 3.257 ± 0.038	3.824ProThr: 3.824 ± 0.048	4.939ProVal: 4.939 ± 0.051	0.943ProTrp: 0.943 ± 0.021	1.355ProTyr: 1.355 ± 0.025	0.0ProXaa: 0.0 ± 0.0
Gln	4.145GlnAla: 4.145 ± 0.039	0.185GlnCys: 0.185 ± 0.009	1.406GlnAsp: 1.406 ± 0.024	1.381GlnGlu: 1.381 ± 0.019	0.953GlnPhe: 0.953 ± 0.021	2.217GlnGly: 2.217 ± 0.032	0.764GlnHis: 0.764 ± 0.018	1.426GlnIle: 1.426 ± 0.021	0.77GlnLys: 0.77 ± 0.018	3.959GlnLeu: 3.959 ± 0.042	0.538GlnMet: 0.538 ± 0.015	0.717GlnAsn: 0.717 ± 0.016	2.075GlnPro: 2.075 ± 0.034	1.54GlnGln: 1.54 ± 0.028	2.511GlnArg: 2.511 ± 0.032	1.686GlnSer: 1.686 ± 0.026	1.864GlnThr: 1.864 ± 0.027	2.94GlnVal: 2.94 ± 0.038	0.596GlnTrp: 0.596 ± 0.017	0.854GlnTyr: 0.854 ± 0.017	0.0GlnXaa: 0.0 ± 0.0
Arg	7.552ArgAla: 7.552 ± 0.067	0.465ArgCys: 0.465 ± 0.014	3.791ArgAsp: 3.791 ± 0.042	3.757ArgGlu: 3.757 ± 0.044	2.252ArgPhe: 2.252 ± 0.03	4.825ArgGly: 4.825 ± 0.042	1.517ArgHis: 1.517 ± 0.027	3.28ArgIle: 3.28 ± 0.032	1.742ArgLys: 1.742 ± 0.025	7.655ArgLeu: 7.655 ± 0.07	1.548ArgMet: 1.548 ± 0.024	1.578ArgAsn: 1.578 ± 0.026	3.88ArgPro: 3.88 ± 0.039	2.399ArgGln: 2.399 ± 0.031	6.265ArgArg: 6.265 ± 0.058	3.979ArgSer: 3.979 ± 0.043	4.592ArgThr: 4.592 ± 0.05	5.194ArgVal: 5.194 ± 0.042	1.329ArgTrp: 1.329 ± 0.023	1.793ArgTyr: 1.793 ± 0.026	0.0ArgXaa: 0.0 ± 0.0
Ser	6.726SerAla: 6.726 ± 0.053	0.419SerCys: 0.419 ± 0.014	2.938SerAsp: 2.938 ± 0.034	2.583SerGlu: 2.583 ± 0.033	1.785SerPhe: 1.785 ± 0.025	5.944SerGly: 5.944 ± 0.057	0.991SerHis: 0.991 ± 0.021	2.207SerIle: 2.207 ± 0.026	1.442SerLys: 1.442 ± 0.028	5.173SerLeu: 5.173 ± 0.052	1.218SerMet: 1.218 ± 0.021	1.326SerAsn: 1.326 ± 0.023	3.052SerPro: 3.052 ± 0.037	1.59SerGln: 1.59 ± 0.026	3.617SerArg: 3.617 ± 0.041	3.406SerSer: 3.406 ± 0.044	3.88SerThr: 3.88 ± 0.039	4.512SerVal: 4.512 ± 0.044	1.077SerTrp: 1.077 ± 0.022	1.607SerTyr: 1.607 ± 0.028	0.0SerXaa: 0.0 ± 0.0
Thr	8.676ThrAla: 8.676 ± 0.07	0.441ThrCys: 0.441 ± 0.015	3.793ThrAsp: 3.793 ± 0.036	3.186ThrGlu: 3.186 ± 0.037	2.007ThrPhe: 2.007 ± 0.029	6.327ThrGly: 6.327 ± 0.051	1.166ThrHis: 1.166 ± 0.024	2.649ThrIle: 2.649 ± 0.031	1.694ThrLys: 1.694 ± 0.032	5.872ThrLeu: 5.872 ± 0.054	1.056ThrMet: 1.056 ± 0.02	1.477ThrAsn: 1.477 ± 0.025	4.251ThrPro: 4.251 ± 0.06	1.727ThrGln: 1.727 ± 0.026	3.582ThrArg: 3.582 ± 0.037	3.699ThrSer: 3.699 ± 0.044	4.619ThrThr: 4.619 ± 0.065	6.035ThrVal: 6.035 ± 0.054	1.071ThrTrp: 1.071 ± 0.021	1.658ThrTyr: 1.658 ± 0.033	0.0ThrXaa: 0.0 ± 0.0
Val	10.827ValAla: 10.827 ± 0.077	0.564ValCys: 0.564 ± 0.012	5.339ValAsp: 5.339 ± 0.045	4.651ValGlu: 4.651 ± 0.047	2.414ValPhe: 2.414 ± 0.032	6.807ValGly: 6.807 ± 0.064	1.652ValHis: 1.652 ± 0.027	3.647ValIle: 3.647 ± 0.042	2.063ValLys: 2.063 ± 0.038	9.478ValLeu: 9.478 ± 0.078	1.4ValMet: 1.4 ± 0.025	1.973ValAsn: 1.973 ± 0.033	4.907ValPro: 4.907 ± 0.044	2.587ValGln: 2.587 ± 0.035	5.995ValArg: 5.995 ± 0.048	4.614ValSer: 4.614 ± 0.045	5.654ValThr: 5.654 ± 0.051	8.498ValVal: 8.498 ± 0.073	1.148ValTrp: 1.148 ± 0.021	1.637ValTyr: 1.637 ± 0.026	0.0ValXaa: 0.0 ± 0.0
Trp	1.67TrpAla: 1.67 ± 0.026	0.134TrpCys: 0.134 ± 0.006	0.855TrpAsp: 0.855 ± 0.021	0.668TrpGlu: 0.668 ± 0.016	0.579TrpPhe: 0.579 ± 0.015	1.093TrpGly: 1.093 ± 0.024	0.388TrpHis: 0.388 ± 0.012	0.716TrpIle: 0.716 ± 0.017	0.464TrpLys: 0.464 ± 0.012	1.903TrpLeu: 1.903 ± 0.028	0.35TrpMet: 0.35 ± 0.011	0.548TrpAsn: 0.548 ± 0.015	0.888TrpPro: 0.888 ± 0.017	0.733TrpGln: 0.733 ± 0.016	1.254TrpArg: 1.254 ± 0.02	1.175TrpSer: 1.175 ± 0.023	1.169TrpThr: 1.169 ± 0.022	1.111TrpVal: 1.111 ± 0.02	0.391TrpTrp: 0.391 ± 0.013	0.39TrpTyr: 0.39 ± 0.013	0.0TrpXaa: 0.0 ± 0.0
Tyr	2.73TyrAla: 2.73 ± 0.031	0.195TyrCys: 0.195 ± 0.008	1.864TyrAsp: 1.864 ± 0.035	1.132TyrGlu: 1.132 ± 0.018	0.778TyrPhe: 0.778 ± 0.017	2.319TyrGly: 2.319 ± 0.028	0.427TyrHis: 0.427 ± 0.013	0.603TyrIle: 0.603 ± 0.013	0.54TyrLys: 0.54 ± 0.017	2.421TyrLeu: 2.421 ± 0.029	0.26TyrMet: 0.26 ± 0.009	0.584TyrAsn: 0.584 ± 0.018	1.217TyrPro: 1.217 ± 0.021	0.833TyrGln: 0.833 ± 0.021	1.808TyrArg: 1.808 ± 0.03	1.25TyrSer: 1.25 ± 0.028	1.339TyrThr: 1.339 ± 0.025	1.938TyrVal: 1.938 ± 0.028	0.427TyrTrp: 0.427 ± 0.014	0.597TyrTyr: 0.597 ± 0.017	0.0TyrXaa: 0.0 ± 0.0
Xaa	0.0XaaAla: 0.0 ± 0.0	0.0XaaCys: 0.0 ± 0.0	0.0XaaAsp: 0.0 ± 0.0	0.0XaaGlu: 0.0 ± 0.0	0.0XaaPhe: 0.0 ± 0.0	0.0XaaGly: 0.0 ± 0.0	0.0XaaHis: 0.0 ± 0.0	0.0XaaIle: 0.0 ± 0.0	0.0XaaLys: 0.0 ± 0.0	0.0XaaLeu: 0.0 ± 0.0	0.0XaaMet: 0.0 ± 0.0	0.0XaaAsn: 0.0 ± 0.0	0.0XaaPro: 0.0 ± 0.0	0.0XaaGln: 0.0 ± 0.0	0.0XaaArg: 0.0 ± 0.0	0.0XaaSer: 0.0 ± 0.0	0.0XaaThr: 0.0 ± 0.0	0.0XaaVal: 0.0 ± 0.0	0.0XaaTrp: 0.0 ± 0.0	0.0XaaTyr: 0.0 ± 0.0	0.0XaaXaa: 0.0 ± 0.0

Statistics based on 8290 proteins (2784158 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944

Contact: Lukasz P. Kozlowski

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