Lepeophtheirus salmonis rhabdovirus 127
Average proteome isoelectric point is 6.57
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 5 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A0A1EBK8|A0A0A1EBK8_9RHAB GDP polyribonucleotidyltransferase OS=Lepeophtheirus salmonis rhabdovirus 127 OX=1573761 PE=4 SV=1
MM1 pKa = 6.7 SQKK4 pKa = 10.08 VSNVGAILDD13 pKa = 4.03 KK14 pKa = 10.64 GVSMVRR20 pKa = 11.84 AEE22 pKa = 3.95 GRR24 pKa = 11.84 RR25 pKa = 11.84 AVEE28 pKa = 4.27 DD29 pKa = 3.89 LGEE32 pKa = 4.15 EE33 pKa = 4.42 SPDD36 pKa = 3.09 EE37 pKa = 5.09 DD38 pKa = 4.11 YY39 pKa = 11.35 QEE41 pKa = 4.69 HH42 pKa = 6.49 PKK44 pKa = 10.73 KK45 pKa = 10.34 PFSSTFGTWHH55 pKa = 6.3 QKK57 pKa = 8.47 FQEE60 pKa = 5.08 DD61 pKa = 3.59 INDD64 pKa = 3.43 WGEE67 pKa = 3.63 QRR69 pKa = 11.84 EE70 pKa = 4.4 IQGLDD75 pKa = 3.14 PEE77 pKa = 4.56 PRR79 pKa = 11.84 STYY82 pKa = 10.94 EE83 pKa = 3.66 MLRR86 pKa = 11.84 DD87 pKa = 3.71 PGEE90 pKa = 4.41 EE91 pKa = 3.58 INIYY95 pKa = 10.23 PDD97 pKa = 3.38 TDD99 pKa = 3.78 LSNMGSQGPEE109 pKa = 3.71 VCDD112 pKa = 3.63 LQDD115 pKa = 3.18 FRR117 pKa = 11.84 TVLDD121 pKa = 4.97 EE122 pKa = 4.02 ISLRR126 pKa = 11.84 YY127 pKa = 9.51 NIPRR131 pKa = 11.84 LIIKK135 pKa = 9.84 RR136 pKa = 11.84 DD137 pKa = 3.23 AGRR140 pKa = 11.84 ITWGEE145 pKa = 3.87 AGTGEE150 pKa = 4.36 EE151 pKa = 4.33 PKK153 pKa = 10.27 IPQDD157 pKa = 3.27 VHH159 pKa = 7.79 LVEE162 pKa = 5.22 EE163 pKa = 4.81 INVKK167 pKa = 10.34 PSIGYY172 pKa = 7.41 PKK174 pKa = 10.2 KK175 pKa = 9.94 PKK177 pKa = 10.46 ASINEE182 pKa = 4.01 TRR184 pKa = 11.84 EE185 pKa = 4.05 RR186 pKa = 11.84 NSLEE190 pKa = 3.42 MALIEE195 pKa = 4.68 KK196 pKa = 9.8 RR197 pKa = 11.84 YY198 pKa = 9.33 HH199 pKa = 5.51 VLGIFDD205 pKa = 3.83 RR206 pKa = 11.84 TKK208 pKa = 10.65 KK209 pKa = 10.42 LSLGIPPIQGVNRR222 pKa = 11.84 LLADD226 pKa = 3.14 IWGEE230 pKa = 4.08 EE231 pKa = 3.98 KK232 pKa = 11.18 AMDD235 pKa = 3.97 DD236 pKa = 4.47 MIRR239 pKa = 11.84 EE240 pKa = 5.01 LYY242 pKa = 9.58 RR243 pKa = 11.84 HH244 pKa = 6.24 HH245 pKa = 7.29 PNMRR249 pKa = 11.84 RR250 pKa = 11.84 LSRR253 pKa = 11.84 SFNFKK258 pKa = 10.47 FIQEE262 pKa = 4.23 SS263 pKa = 3.44
Molecular weight: 30.34 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 5.197
IPC2_protein 5.194
IPC_protein 5.13
Toseland 5.105
ProMoST 5.27
Dawson 5.13
Bjellqvist 5.258
Wikipedia 4.991
Rodwell 5.054
Grimsley 5.029
Solomon 5.13
Lehninger 5.08
Nozaki 5.258
DTASelect 5.385
Thurlkill 5.092
EMBOSS 5.041
Sillero 5.334
Patrickios 4.418
IPC_peptide 5.13
IPC2_peptide 5.321
IPC2.peptide.svr19 5.337
Protein with the highest isoelectric point:
>tr|A0A0A1E924|A0A0A1E924_9RHAB Phosphoprotein OS=Lepeophtheirus salmonis rhabdovirus 127 OX=1573761 PE=4 SV=1
MM1 pKa = 7.23 LRR3 pKa = 11.84 KK4 pKa = 9.28 AALALRR10 pKa = 11.84 GGNKK14 pKa = 10.01 EE15 pKa = 3.92 EE16 pKa = 4.63 DD17 pKa = 3.4 RR18 pKa = 11.84 HH19 pKa = 6.28 PVTSWSSPIQSPSAPMDD36 pKa = 3.57 SRR38 pKa = 11.84 LLTVNLKK45 pKa = 8.37 VTAHH49 pKa = 5.46 MSVRR53 pKa = 11.84 TTNKK57 pKa = 9.82 NIVFSDD63 pKa = 3.58 VLLACTDD70 pKa = 3.09 IMDD73 pKa = 4.36 TYY75 pKa = 10.45 DD76 pKa = 4.46 GPMEE80 pKa = 4.01 TLGFYY85 pKa = 10.14 WIMILVLVKK94 pKa = 10.36 RR95 pKa = 11.84 LKK97 pKa = 10.44 SLRR100 pKa = 11.84 GNEE103 pKa = 3.9 GTQDD107 pKa = 3.58 FVSSVSEE114 pKa = 4.31 PISFLTDD121 pKa = 3.13 ISSPYY126 pKa = 10.71 LKK128 pKa = 9.76 TFPTTAFSFGRR139 pKa = 11.84 EE140 pKa = 3.69 FRR142 pKa = 11.84 AGKK145 pKa = 10.24 VSGNASVSFSEE156 pKa = 4.35 TRR158 pKa = 11.84 AVTTSLEE165 pKa = 4.81 LILQSLGLVNDD176 pKa = 4.32 HH177 pKa = 6.86 LGSALSALKK186 pKa = 10.43 IPAEE190 pKa = 4.3 RR191 pKa = 11.84 IKK193 pKa = 10.98 GSLVIHH199 pKa = 6.13 MALRR203 pKa = 11.84 DD204 pKa = 4.16 SFSHH208 pKa = 6.19 IQPATLKK215 pKa = 8.32 EE216 pKa = 4.23 TSDD219 pKa = 3.41
Molecular weight: 23.98 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 7.944
IPC2_protein 7.907
IPC_protein 8.112
Toseland 8.712
ProMoST 8.361
Dawson 8.858
Bjellqvist 8.712
Wikipedia 8.902
Rodwell 8.975
Grimsley 8.58
Solomon 9.18
Lehninger 9.18
Nozaki 8.902
DTASelect 8.668
Thurlkill 8.799
EMBOSS 9.077
Sillero 8.975
Patrickios 5.194
IPC_peptide 9.18
IPC2_peptide 7.527
IPC2.peptide.svr19 7.502
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
5
0
5
3586
219
2096
717.2
82.0
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
4.183 ± 0.533
1.422 ± 0.382
6.191 ± 0.119
6.219 ± 0.38
4.406 ± 0.151
5.8 ± 0.199
2.566 ± 0.254
6.804 ± 0.199
6.219 ± 0.566
10.318 ± 0.674
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.566 ± 0.12
4.044 ± 0.185
4.713 ± 0.508
2.817 ± 0.146
6.414 ± 0.397
8.645 ± 0.411
6.079 ± 0.452
5.577 ± 0.407
2.091 ± 0.185
2.928 ± 0.279
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here