Arthrobacter sp. CAU 1506
Average proteome isoelectric point is 6.1
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 3726 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A4U0HAT1|A0A4U0HAT1_9MICC PEP-utilizers domain-containing protein OS=Arthrobacter sp. CAU 1506 OX=2560052 GN=E4J89_12170 PE=4 SV=1
MM1 pKa = 7.44 AKK3 pKa = 9.87 ISFHH7 pKa = 7.02 HH8 pKa = 7.01 SDD10 pKa = 3.44 GDD12 pKa = 3.87 VDD14 pKa = 4.61 VLDD17 pKa = 4.24 VDD19 pKa = 4.46 PGTSLMRR26 pKa = 11.84 AAVTNGVDD34 pKa = 4.61 GIVGEE39 pKa = 4.64 CGGQAMCATCHH50 pKa = 5.61 VYY52 pKa = 10.14 VRR54 pKa = 11.84 PEE56 pKa = 4.03 YY57 pKa = 10.96 LDD59 pKa = 3.93 GLPDD63 pKa = 3.73 IGDD66 pKa = 4.25 DD67 pKa = 3.43 EE68 pKa = 6.62 GEE70 pKa = 4.17 MLEE73 pKa = 4.57 CTAGPRR79 pKa = 11.84 DD80 pKa = 3.67 EE81 pKa = 4.41 ARR83 pKa = 11.84 SRR85 pKa = 11.84 LGCQLTAGEE94 pKa = 4.95 DD95 pKa = 3.36 FDD97 pKa = 6.72 AIEE100 pKa = 4.38 VDD102 pKa = 3.97 VPDD105 pKa = 4.57 DD106 pKa = 3.59 QVV108 pKa = 2.93
Molecular weight: 11.43 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.731
IPC2_protein 3.821
IPC_protein 3.808
Toseland 3.592
ProMoST 3.986
Dawson 3.808
Bjellqvist 3.973
Wikipedia 3.77
Rodwell 3.643
Grimsley 3.503
Solomon 3.795
Lehninger 3.757
Nozaki 3.935
DTASelect 4.177
Thurlkill 3.656
EMBOSS 3.77
Sillero 3.935
Patrickios 1.901
IPC_peptide 3.795
IPC2_peptide 3.91
IPC2.peptide.svr19 3.844
Protein with the highest isoelectric point:
>tr|A0A4U0HCX8|A0A4U0HCX8_9MICC o-succinylbenzoate synthase OS=Arthrobacter sp. CAU 1506 OX=2560052 GN=menC PE=3 SV=1
MM1 pKa = 7.4 GSVIKK6 pKa = 10.42 KK7 pKa = 8.47 RR8 pKa = 11.84 RR9 pKa = 11.84 KK10 pKa = 9.22 RR11 pKa = 11.84 MAKK14 pKa = 9.41 KK15 pKa = 9.87 KK16 pKa = 9.7 HH17 pKa = 5.84 RR18 pKa = 11.84 KK19 pKa = 8.56 LLRR22 pKa = 11.84 KK23 pKa = 7.78 TRR25 pKa = 11.84 HH26 pKa = 3.65 QRR28 pKa = 11.84 RR29 pKa = 11.84 NKK31 pKa = 9.78 KK32 pKa = 9.85
Molecular weight: 4.08 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.493
IPC2_protein 11.14
IPC_protein 12.691
Toseland 12.866
ProMoST 13.349
Dawson 12.866
Bjellqvist 12.852
Wikipedia 13.334
Rodwell 12.735
Grimsley 12.91
Solomon 13.349
Lehninger 13.261
Nozaki 12.866
DTASelect 12.852
Thurlkill 12.866
EMBOSS 13.364
Sillero 12.866
Patrickios 12.457
IPC_peptide 13.349
IPC2_peptide 12.34
IPC2.peptide.svr19 9.071
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
3726
0
3726
1222125
30
2032
328.0
35.17
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
13.08 ± 0.054
0.623 ± 0.01
5.739 ± 0.036
5.863 ± 0.039
3.155 ± 0.026
9.123 ± 0.037
2.095 ± 0.017
4.25 ± 0.032
2.615 ± 0.025
10.275 ± 0.047
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.063 ± 0.017
2.338 ± 0.021
5.457 ± 0.03
3.33 ± 0.018
6.74 ± 0.038
5.724 ± 0.026
5.676 ± 0.026
8.322 ± 0.033
1.44 ± 0.017
2.091 ± 0.021
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here