Zymomonas sp.
Average proteome isoelectric point is 6.39
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 1056 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A519E6Z4|A0A519E6Z4_9SPHN Coproporphyrinogen III oxidase (Fragment) OS=Zymomonas sp. OX=2068624 GN=EOP94_02850 PE=4 SV=1
MM1 pKa = 8.88 IMEE4 pKa = 4.15 QPADD8 pKa = 3.88 YY9 pKa = 11.36 DD10 pKa = 3.48 HH11 pKa = 7.3 LLQHH15 pKa = 6.55 AQRR18 pKa = 11.84 LFPGAQVDD26 pKa = 3.36 ITYY29 pKa = 9.78 TEE31 pKa = 4.7 DD32 pKa = 3.56 EE33 pKa = 4.96 IIHH36 pKa = 6.54 IDD38 pKa = 3.61 VDD40 pKa = 3.11 GHH42 pKa = 6.73 RR43 pKa = 11.84 YY44 pKa = 6.73 TFEE47 pKa = 4.84 IGSDD51 pKa = 3.24 DD52 pKa = 4.19 DD53 pKa = 5.33 EE54 pKa = 5.03 YY55 pKa = 11.95 VFDD58 pKa = 5.67 DD59 pKa = 4.25 RR60 pKa = 11.84 STCFSIPLFLDD71 pKa = 3.43 ATWDD75 pKa = 3.95 FSS77 pKa = 3.9
Molecular weight: 9.03 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.714
IPC2_protein 3.834
IPC_protein 3.808
Toseland 3.592
ProMoST 3.999
Dawson 3.821
Bjellqvist 3.973
Wikipedia 3.795
Rodwell 3.643
Grimsley 3.503
Solomon 3.808
Lehninger 3.77
Nozaki 3.948
DTASelect 4.215
Thurlkill 3.668
EMBOSS 3.795
Sillero 3.935
Patrickios 0.896
IPC_peptide 3.795
IPC2_peptide 3.91
IPC2.peptide.svr19 3.831
Protein with the highest isoelectric point:
>tr|A0A519ED50|A0A519ED50_9SPHN ParB/RepB/Spo0J family partition protein OS=Zymomonas sp. OX=2068624 GN=EOP94_00420 PE=3 SV=1
MM1 pKa = 7.24 MGLEE5 pKa = 4.08 GRR7 pKa = 11.84 IAQLKK12 pKa = 5.55 PTSVGGHH19 pKa = 3.76 VTGYY23 pKa = 10.44 LVLGGTSRR31 pKa = 11.84 GFAFGGPEE39 pKa = 3.87 FFLDD43 pKa = 3.98 LDD45 pKa = 4.4 RR46 pKa = 11.84 FPSRR50 pKa = 11.84 PWLSRR55 pKa = 11.84 SCSMSCSARR64 pKa = 11.84 FRR66 pKa = 11.84 ASPRR70 pKa = 11.84 HH71 pKa = 5.72 RR72 pKa = 11.84 RR73 pKa = 11.84 LDD75 pKa = 3.73 PLGLRR80 pKa = 11.84 HH81 pKa = 6.29 GARR84 pKa = 11.84 HH85 pKa = 5.93 RR86 pKa = 11.84 CRR88 pKa = 11.84 EE89 pKa = 3.97 GAGAIADD96 pKa = 3.89 LTGEE100 pKa = 4.19 PGALFVQRR108 pKa = 11.84 YY109 pKa = 8.98 RR110 pKa = 11.84 NLKK113 pKa = 10.54 SYY115 pKa = 10.26 GKK117 pKa = 10.27 SNAVPLLKK125 pKa = 10.68 AEE127 pKa = 4.84 TIRR130 pKa = 11.84 SGEE133 pKa = 3.93 QLAACAAA140 pKa = 4.02
Molecular weight: 15.16 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.348
IPC2_protein 9.604
IPC_protein 10.628
Toseland 10.672
ProMoST 10.526
Dawson 10.774
Bjellqvist 10.57
Wikipedia 11.038
Rodwell 10.804
Grimsley 10.818
Solomon 10.95
Lehninger 10.906
Nozaki 10.701
DTASelect 10.555
Thurlkill 10.687
EMBOSS 11.096
Sillero 10.716
Patrickios 10.54
IPC_peptide 10.95
IPC2_peptide 9.94
IPC2.peptide.svr19 8.571
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
1056
0
1056
225047
25
988
213.1
23.33
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
11.494 ± 0.097
0.874 ± 0.028
6.085 ± 0.056
5.784 ± 0.065
3.811 ± 0.055
8.017 ± 0.082
2.123 ± 0.043
5.269 ± 0.056
3.297 ± 0.065
9.912 ± 0.091
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.515 ± 0.043
2.774 ± 0.055
4.871 ± 0.054
3.284 ± 0.044
7.381 ± 0.085
5.947 ± 0.06
5.502 ± 0.068
7.309 ± 0.068
1.352 ± 0.033
2.401 ± 0.044
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here