Amino acid dipepetide frequency for Escherichia phage muut

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
4.579AlaAla: 4.579 ± 0.45
0.713AlaCys: 0.713 ± 0.13
3.521AlaAsp: 3.521 ± 0.291
4.28AlaGlu: 4.28 ± 0.428
2.301AlaPhe: 2.301 ± 0.258
4.464AlaGly: 4.464 ± 0.368
1.289AlaHis: 1.289 ± 0.189
4.28AlaIle: 4.28 ± 0.309
5.615AlaLys: 5.615 ± 0.383
5.661AlaLeu: 5.661 ± 0.338
1.979AlaMet: 1.979 ± 0.257
3.175AlaAsn: 3.175 ± 0.327
1.795AlaPro: 1.795 ± 0.212
2.462AlaGln: 2.462 ± 0.198
2.508AlaArg: 2.508 ± 0.245
4.027AlaSer: 4.027 ± 0.315
4.464AlaThr: 4.464 ± 0.431
3.682AlaVal: 3.682 ± 0.34
1.128AlaTrp: 1.128 ± 0.198
2.922AlaTyr: 2.922 ± 0.249
0.0AlaXaa: 0.0 ± 0.0
Cys
0.966CysAla: 0.966 ± 0.15
0.23CysCys: 0.23 ± 0.071
0.943CysAsp: 0.943 ± 0.187
0.874CysGlu: 0.874 ± 0.144
1.082CysPhe: 1.082 ± 0.173
1.035CysGly: 1.035 ± 0.144
0.253CysHis: 0.253 ± 0.067
0.989CysIle: 0.989 ± 0.12
0.874CysLys: 0.874 ± 0.153
1.289CysLeu: 1.289 ± 0.193
0.253CysMet: 0.253 ± 0.077
0.506CysAsn: 0.506 ± 0.101
0.598CysPro: 0.598 ± 0.149
0.414CysGln: 0.414 ± 0.098
0.621CysArg: 0.621 ± 0.164
0.782CysSer: 0.782 ± 0.139
0.621CysThr: 0.621 ± 0.109
0.966CysVal: 0.966 ± 0.153
0.276CysTrp: 0.276 ± 0.078
0.736CysTyr: 0.736 ± 0.127
0.0CysXaa: 0.0 ± 0.0
Asp
3.935AspAla: 3.935 ± 0.356
0.667AspCys: 0.667 ± 0.129
3.797AspAsp: 3.797 ± 0.293
3.935AspGlu: 3.935 ± 0.285
3.291AspPhe: 3.291 ± 0.317
4.832AspGly: 4.832 ± 0.392
1.473AspHis: 1.473 ± 0.183
4.533AspIle: 4.533 ± 0.297
4.441AspLys: 4.441 ± 0.346
5.638AspLeu: 5.638 ± 0.408
1.749AspMet: 1.749 ± 0.194
3.774AspAsn: 3.774 ± 0.313
2.807AspPro: 2.807 ± 0.258
2.025AspGln: 2.025 ± 0.239
2.531AspArg: 2.531 ± 0.221
2.991AspSer: 2.991 ± 0.255
3.544AspThr: 3.544 ± 0.286
4.694AspVal: 4.694 ± 0.357
1.289AspTrp: 1.289 ± 0.195
3.383AspTyr: 3.383 ± 0.291
0.0AspXaa: 0.0 ± 0.0
Glu
4.97GluAla: 4.97 ± 0.402
1.035GluCys: 1.035 ± 0.174
5.822GluAsp: 5.822 ± 0.402
7.732GluGlu: 7.732 ± 0.592
2.784GluPhe: 2.784 ± 0.267
4.119GluGly: 4.119 ± 0.311
1.542GluHis: 1.542 ± 0.193
5.062GluIle: 5.062 ± 0.447
5.431GluLys: 5.431 ± 0.337
4.832GluLeu: 4.832 ± 0.34
2.508GluMet: 2.508 ± 0.259
3.958GluAsn: 3.958 ± 0.314
1.565GluPro: 1.565 ± 0.217
2.853GluGln: 2.853 ± 0.267
2.991GluArg: 2.991 ± 0.241
3.337GluSer: 3.337 ± 0.29
3.958GluThr: 3.958 ± 0.318
5.085GluVal: 5.085 ± 0.413
1.427GluTrp: 1.427 ± 0.199
2.784GluTyr: 2.784 ± 0.272
0.0GluXaa: 0.0 ± 0.0
Phe
2.646PheAla: 2.646 ± 0.255
0.759PheCys: 0.759 ± 0.141
3.613PheAsp: 3.613 ± 0.362
2.554PheGlu: 2.554 ± 0.226
2.209PhePhe: 2.209 ± 0.266
2.876PheGly: 2.876 ± 0.269
0.575PheHis: 0.575 ± 0.112
2.83PheIle: 2.83 ± 0.227
3.314PheLys: 3.314 ± 0.257
3.452PheLeu: 3.452 ± 0.308
1.058PheMet: 1.058 ± 0.154
2.554PheAsn: 2.554 ± 0.206
1.358PhePro: 1.358 ± 0.182
1.335PheGln: 1.335 ± 0.191
1.611PheArg: 1.611 ± 0.213
3.406PheSer: 3.406 ± 0.345
2.899PheThr: 2.899 ± 0.248
3.083PheVal: 3.083 ± 0.3
0.621PheTrp: 0.621 ± 0.119
1.956PheTyr: 1.956 ± 0.239
0.0PheXaa: 0.0 ± 0.0
Gly
3.613GlyAla: 3.613 ± 0.299
1.082GlyCys: 1.082 ± 0.168
4.602GlyAsp: 4.602 ± 0.463
4.464GlyGlu: 4.464 ± 0.339
3.36GlyPhe: 3.36 ± 0.27
4.119GlyGly: 4.119 ± 0.437
1.243GlyHis: 1.243 ± 0.169
3.843GlyIle: 3.843 ± 0.253
5.062GlyLys: 5.062 ± 0.371
5.016GlyLeu: 5.016 ± 0.313
1.565GlyMet: 1.565 ± 0.168
3.498GlyAsn: 3.498 ± 0.361
0.414GlyPro: 0.414 ± 0.149
1.91GlyGln: 1.91 ± 0.216
2.416GlyArg: 2.416 ± 0.23
3.682GlySer: 3.682 ± 0.25
3.774GlyThr: 3.774 ± 0.403
5.431GlyVal: 5.431 ± 0.418
1.035GlyTrp: 1.035 ± 0.154
3.383GlyTyr: 3.383 ± 0.289
0.0GlyXaa: 0.0 ± 0.0
His
1.035HisAla: 1.035 ± 0.159
0.46HisCys: 0.46 ± 0.098
1.128HisAsp: 1.128 ± 0.16
0.92HisGlu: 0.92 ± 0.135
1.128HisPhe: 1.128 ± 0.145
1.266HisGly: 1.266 ± 0.21
0.276HisHis: 0.276 ± 0.084
1.22HisIle: 1.22 ± 0.188
1.105HisLys: 1.105 ± 0.168
1.496HisLeu: 1.496 ± 0.212
0.552HisMet: 0.552 ± 0.118
1.197HisAsn: 1.197 ± 0.177
0.874HisPro: 0.874 ± 0.147
0.391HisGln: 0.391 ± 0.094
1.058HisArg: 1.058 ± 0.169
0.966HisSer: 0.966 ± 0.149
1.058HisThr: 1.058 ± 0.15
1.289HisVal: 1.289 ± 0.182
0.322HisTrp: 0.322 ± 0.095
1.174HisTyr: 1.174 ± 0.197
0.0HisXaa: 0.0 ± 0.0
Ile
4.694IleAla: 4.694 ± 0.311
1.058IleCys: 1.058 ± 0.193
4.257IleAsp: 4.257 ± 0.35
4.809IleGlu: 4.809 ± 0.362
2.577IlePhe: 2.577 ± 0.265
3.544IleGly: 3.544 ± 0.26
1.058IleHis: 1.058 ± 0.168
4.004IleIle: 4.004 ± 0.343
4.441IleLys: 4.441 ± 0.379
4.947IleLeu: 4.947 ± 0.386
1.22IleMet: 1.22 ± 0.167
3.728IleAsn: 3.728 ± 0.351
2.899IlePro: 2.899 ± 0.274
2.347IleGln: 2.347 ± 0.21
2.209IleArg: 2.209 ± 0.194
3.912IleSer: 3.912 ± 0.315
4.533IleThr: 4.533 ± 0.28
5.615IleVal: 5.615 ± 0.328
0.667IleTrp: 0.667 ± 0.128
2.531IleTyr: 2.531 ± 0.232
0.0IleXaa: 0.0 ± 0.0
Lys
5.615LysAla: 5.615 ± 0.426
0.828LysCys: 0.828 ± 0.138
4.487LysAsp: 4.487 ± 0.36
6.765LysGlu: 6.765 ± 0.446
2.485LysPhe: 2.485 ± 0.237
4.648LysGly: 4.648 ± 0.476
1.795LysHis: 1.795 ± 0.188
4.51LysIle: 4.51 ± 0.316
4.671LysLys: 4.671 ± 0.46
5.292LysLeu: 5.292 ± 0.316
2.393LysMet: 2.393 ± 0.228
3.268LysAsn: 3.268 ± 0.328
2.163LysPro: 2.163 ± 0.218
2.715LysGln: 2.715 ± 0.223
3.452LysArg: 3.452 ± 0.376
3.59LysSer: 3.59 ± 0.287
4.027LysThr: 4.027 ± 0.302
4.441LysVal: 4.441 ± 0.297
1.22LysTrp: 1.22 ± 0.177
3.083LysTyr: 3.083 ± 0.319
0.0LysXaa: 0.0 ± 0.0
Leu
5.408LeuAla: 5.408 ± 0.319
1.058LeuCys: 1.058 ± 0.147
5.408LeuAsp: 5.408 ± 0.339
5.822LeuGlu: 5.822 ± 0.388
3.06LeuPhe: 3.06 ± 0.307
4.74LeuGly: 4.74 ± 0.371
1.634LeuHis: 1.634 ± 0.229
4.441LeuIle: 4.441 ± 0.354
5.638LeuLys: 5.638 ± 0.416
6.558LeuLeu: 6.558 ± 0.374
1.864LeuMet: 1.864 ± 0.206
4.234LeuAsn: 4.234 ± 0.356
3.337LeuPro: 3.337 ± 0.284
2.83LeuGln: 2.83 ± 0.23
3.659LeuArg: 3.659 ± 0.366
5.776LeuSer: 5.776 ± 0.351
5.385LeuThr: 5.385 ± 0.404
5.246LeuVal: 5.246 ± 0.356
0.92LeuTrp: 0.92 ± 0.174
3.337LeuTyr: 3.337 ± 0.268
0.0LeuXaa: 0.0 ± 0.0
Met
1.979MetAla: 1.979 ± 0.21
0.368MetCys: 0.368 ± 0.087
1.035MetAsp: 1.035 ± 0.153
2.071MetGlu: 2.071 ± 0.215
1.243MetPhe: 1.243 ± 0.191
1.404MetGly: 1.404 ± 0.175
0.483MetHis: 0.483 ± 0.109
2.117MetIle: 2.117 ± 0.228
2.117MetLys: 2.117 ± 0.207
2.462MetLeu: 2.462 ± 0.245
0.667MetMet: 0.667 ± 0.137
0.92MetAsn: 0.92 ± 0.167
0.782MetPro: 0.782 ± 0.144
0.897MetGln: 0.897 ± 0.137
1.012MetArg: 1.012 ± 0.141
2.117MetSer: 2.117 ± 0.272
1.542MetThr: 1.542 ± 0.182
1.703MetVal: 1.703 ± 0.201
0.368MetTrp: 0.368 ± 0.094
0.966MetTyr: 0.966 ± 0.119
0.0MetXaa: 0.0 ± 0.0
Asn
3.014AsnAla: 3.014 ± 0.23
0.667AsnCys: 0.667 ± 0.111
2.393AsnAsp: 2.393 ± 0.277
2.968AsnGlu: 2.968 ± 0.291
2.485AsnPhe: 2.485 ± 0.268
3.866AsnGly: 3.866 ± 0.345
0.92AsnHis: 0.92 ± 0.139
4.648AsnIle: 4.648 ± 0.304
3.912AsnLys: 3.912 ± 0.322
3.751AsnLeu: 3.751 ± 0.267
1.634AsnMet: 1.634 ± 0.2
3.106AsnAsn: 3.106 ± 0.306
2.439AsnPro: 2.439 ± 0.288
1.473AsnGln: 1.473 ± 0.204
1.795AsnArg: 1.795 ± 0.204
3.152AsnSer: 3.152 ± 0.297
3.866AsnThr: 3.866 ± 0.301
3.613AsnVal: 3.613 ± 0.262
0.598AsnTrp: 0.598 ± 0.142
2.439AsnTyr: 2.439 ± 0.214
0.0AsnXaa: 0.0 ± 0.0
Pro
2.025ProAla: 2.025 ± 0.194
0.483ProCys: 0.483 ± 0.098
2.577ProAsp: 2.577 ± 0.22
3.175ProGlu: 3.175 ± 0.247
1.749ProPhe: 1.749 ± 0.197
1.657ProGly: 1.657 ± 0.237
0.644ProHis: 0.644 ± 0.135
1.772ProIle: 1.772 ± 0.214
2.14ProLys: 2.14 ± 0.213
2.002ProLeu: 2.002 ± 0.201
0.736ProMet: 0.736 ± 0.113
1.91ProAsn: 1.91 ± 0.252
0.92ProPro: 0.92 ± 0.149
0.782ProGln: 0.782 ± 0.147
1.174ProArg: 1.174 ± 0.162
2.577ProSer: 2.577 ± 0.227
2.439ProThr: 2.439 ± 0.291
2.761ProVal: 2.761 ± 0.256
0.345ProTrp: 0.345 ± 0.097
1.841ProTyr: 1.841 ± 0.218
0.0ProXaa: 0.0 ± 0.0
Gln
2.186GlnAla: 2.186 ± 0.255
0.414GlnCys: 0.414 ± 0.11
2.025GlnAsp: 2.025 ± 0.221
2.945GlnGlu: 2.945 ± 0.283
1.542GlnPhe: 1.542 ± 0.149
2.278GlnGly: 2.278 ± 0.324
0.598GlnHis: 0.598 ± 0.107
2.14GlnIle: 2.14 ± 0.187
1.956GlnLys: 1.956 ± 0.212
2.393GlnLeu: 2.393 ± 0.225
1.082GlnMet: 1.082 ± 0.149
1.312GlnAsn: 1.312 ± 0.185
0.874GlnPro: 0.874 ± 0.139
1.427GlnGln: 1.427 ± 0.174
1.312GlnArg: 1.312 ± 0.197
1.703GlnSer: 1.703 ± 0.209
1.818GlnThr: 1.818 ± 0.207
2.209GlnVal: 2.209 ± 0.238
0.621GlnTrp: 0.621 ± 0.112
1.726GlnTyr: 1.726 ± 0.198
0.0GlnXaa: 0.0 ± 0.0
Arg
2.117ArgAla: 2.117 ± 0.196
0.759ArgCys: 0.759 ± 0.143
2.807ArgAsp: 2.807 ± 0.246
2.646ArgGlu: 2.646 ± 0.267
1.542ArgPhe: 1.542 ± 0.187
2.485ArgGly: 2.485 ± 0.285
0.69ArgHis: 0.69 ± 0.12
2.761ArgIle: 2.761 ± 0.265
3.175ArgLys: 3.175 ± 0.292
3.36ArgLeu: 3.36 ± 0.298
1.128ArgMet: 1.128 ± 0.173
2.485ArgAsn: 2.485 ± 0.216
1.174ArgPro: 1.174 ± 0.142
1.473ArgGln: 1.473 ± 0.261
1.68ArgArg: 1.68 ± 0.239
2.462ArgSer: 2.462 ± 0.217
1.956ArgThr: 1.956 ± 0.199
2.646ArgVal: 2.646 ± 0.234
0.483ArgTrp: 0.483 ± 0.103
1.703ArgTyr: 1.703 ± 0.16
0.0ArgXaa: 0.0 ± 0.0
Ser
4.234SerAla: 4.234 ± 0.349
0.759SerCys: 0.759 ± 0.12
3.429SerAsp: 3.429 ± 0.277
3.889SerGlu: 3.889 ± 0.305
2.968SerPhe: 2.968 ± 0.238
4.625SerGly: 4.625 ± 0.361
0.943SerHis: 0.943 ± 0.131
3.751SerIle: 3.751 ± 0.284
4.257SerLys: 4.257 ± 0.305
5.062SerLeu: 5.062 ± 0.376
1.427SerMet: 1.427 ± 0.191
3.337SerAsn: 3.337 ± 0.268
2.255SerPro: 2.255 ± 0.261
1.496SerGln: 1.496 ± 0.215
2.531SerArg: 2.531 ± 0.248
3.981SerSer: 3.981 ± 0.42
3.36SerThr: 3.36 ± 0.33
4.142SerVal: 4.142 ± 0.32
0.782SerTrp: 0.782 ± 0.141
2.692SerTyr: 2.692 ± 0.241
0.0SerXaa: 0.0 ± 0.0
Thr
3.728ThrAla: 3.728 ± 0.384
0.828ThrCys: 0.828 ± 0.121
3.36ThrAsp: 3.36 ± 0.37
4.625ThrGlu: 4.625 ± 0.362
3.014ThrPhe: 3.014 ± 0.27
4.349ThrGly: 4.349 ± 0.446
0.989ThrHis: 0.989 ± 0.145
3.797ThrIle: 3.797 ± 0.264
4.303ThrLys: 4.303 ± 0.3
5.615ThrLeu: 5.615 ± 0.375
1.473ThrMet: 1.473 ± 0.183
2.83ThrAsn: 2.83 ± 0.232
2.83ThrPro: 2.83 ± 0.232
1.979ThrGln: 1.979 ± 0.176
1.956ThrArg: 1.956 ± 0.192
3.06ThrSer: 3.06 ± 0.3
3.521ThrThr: 3.521 ± 0.444
4.717ThrVal: 4.717 ± 0.465
0.966ThrTrp: 0.966 ± 0.164
2.922ThrTyr: 2.922 ± 0.262
0.0ThrXaa: 0.0 ± 0.0
Val
4.211ValAla: 4.211 ± 0.336
1.105ValCys: 1.105 ± 0.181
5.454ValAsp: 5.454 ± 0.365
5.085ValGlu: 5.085 ± 0.381
2.991ValPhe: 2.991 ± 0.357
3.705ValGly: 3.705 ± 0.364
1.335ValHis: 1.335 ± 0.163
4.809ValIle: 4.809 ± 0.311
5.223ValLys: 5.223 ± 0.38
5.776ValLeu: 5.776 ± 0.376
1.45ValMet: 1.45 ± 0.181
3.682ValAsn: 3.682 ± 0.322
2.485ValPro: 2.485 ± 0.217
1.841ValGln: 1.841 ± 0.274
2.508ValArg: 2.508 ± 0.264
4.648ValSer: 4.648 ± 0.357
4.671ValThr: 4.671 ± 0.385
5.569ValVal: 5.569 ± 0.469
0.897ValTrp: 0.897 ± 0.158
3.705ValTyr: 3.705 ± 0.319
0.0ValXaa: 0.0 ± 0.0
Trp
0.598TrpAla: 0.598 ± 0.112
0.23TrpCys: 0.23 ± 0.071
1.312TrpAsp: 1.312 ± 0.212
1.197TrpGlu: 1.197 ± 0.156
0.943TrpPhe: 0.943 ± 0.173
0.69TrpGly: 0.69 ± 0.122
0.391TrpHis: 0.391 ± 0.1
1.082TrpIle: 1.082 ± 0.206
1.058TrpLys: 1.058 ± 0.157
1.588TrpLeu: 1.588 ± 0.184
0.483TrpMet: 0.483 ± 0.125
0.713TrpAsn: 0.713 ± 0.127
0.276TrpPro: 0.276 ± 0.075
0.414TrpGln: 0.414 ± 0.122
0.575TrpArg: 0.575 ± 0.105
0.897TrpSer: 0.897 ± 0.142
0.644TrpThr: 0.644 ± 0.135
0.989TrpVal: 0.989 ± 0.135
0.345TrpTrp: 0.345 ± 0.102
0.575TrpTyr: 0.575 ± 0.11
0.0TrpXaa: 0.0 ± 0.0
Tyr
3.314TyrAla: 3.314 ± 0.271
0.805TyrCys: 0.805 ± 0.142
3.36TyrAsp: 3.36 ± 0.264
3.037TyrGlu: 3.037 ± 0.239
1.956TyrPhe: 1.956 ± 0.203
2.807TyrGly: 2.807 ± 0.21
0.782TyrHis: 0.782 ± 0.148
2.462TyrIle: 2.462 ± 0.209
2.715TyrLys: 2.715 ± 0.273
4.165TyrLeu: 4.165 ± 0.314
1.012TyrMet: 1.012 ± 0.144
2.554TyrAsn: 2.554 ± 0.252
1.864TyrPro: 1.864 ± 0.219
1.496TyrGln: 1.496 ± 0.193
1.933TyrArg: 1.933 ± 0.177
2.853TyrSer: 2.853 ± 0.319
2.784TyrThr: 2.784 ± 0.253
3.268TyrVal: 3.268 ± 0.266
0.69TyrTrp: 0.69 ± 0.137
2.14TyrTyr: 2.14 ± 0.253
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 239 proteins (43459 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski