Chlamydia phage 1 (Bacteriophage Chp1)

Taxonomy: Viruses; Monodnaviria; Sangervirae; Phixviricota; Malgrandaviricetes; Petitvirales; Microviridae; Gokushovirinae; Chlamydiamicrovirus

Average proteome isoelectric point is 6.82

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 8 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>sp|P19194|B_BPCHP Internal scaffolding protein VP3 OS=Chlamydia phage 1 OX=2003327 GN=ORF3 PE=1 SV=1
MM1 pKa = 7.79KK2 pKa = 10.24FRR4 pKa = 11.84TIYY7 pKa = 10.67DD8 pKa = 3.68EE9 pKa = 4.26EE10 pKa = 5.14RR11 pKa = 11.84PAPVLEE17 pKa = 4.93CKK19 pKa = 10.63DD20 pKa = 3.71EE21 pKa = 4.34SLCLAYY27 pKa = 10.36QCTEE31 pKa = 3.63TSIEE35 pKa = 4.2KK36 pKa = 10.14LVKK39 pKa = 10.12LANQNPSYY47 pKa = 10.84LHH49 pKa = 6.93AFAGDD54 pKa = 3.6PTRR57 pKa = 11.84QPEE60 pKa = 4.22YY61 pKa = 10.8GEE63 pKa = 4.69CPSPLDD69 pKa = 3.57YY70 pKa = 11.06QDD72 pKa = 3.9ALEE75 pKa = 4.16IVARR79 pKa = 11.84GEE81 pKa = 3.74EE82 pKa = 4.16AFYY85 pKa = 11.06SLPANIRR92 pKa = 11.84VNFSNPMEE100 pKa = 4.19FLSWLEE106 pKa = 4.15DD107 pKa = 3.3PANYY111 pKa = 10.56DD112 pKa = 3.52EE113 pKa = 4.54VEE115 pKa = 4.1KK116 pKa = 11.19LGLLDD121 pKa = 3.76PEE123 pKa = 4.34KK124 pKa = 10.88VQIRR128 pKa = 11.84KK129 pKa = 9.74SKK131 pKa = 9.55LQKK134 pKa = 9.79DD135 pKa = 3.73QKK137 pKa = 10.77EE138 pKa = 4.31EE139 pKa = 3.88VSSEE143 pKa = 3.97EE144 pKa = 4.16KK145 pKa = 10.87

Molecular weight: 16.68 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>sp|P19189|REP_BPCHP Replication-associated protein ORF4 OS=Chlamydia phage 1 OX=2003327 GN=ORF4 PE=3 SV=1
MM1 pKa = 6.77VRR3 pKa = 11.84RR4 pKa = 11.84RR5 pKa = 11.84RR6 pKa = 11.84LRR8 pKa = 11.84RR9 pKa = 11.84RR10 pKa = 11.84ISRR13 pKa = 11.84RR14 pKa = 11.84IFRR17 pKa = 11.84RR18 pKa = 11.84TVARR22 pKa = 11.84VGRR25 pKa = 11.84RR26 pKa = 11.84RR27 pKa = 11.84RR28 pKa = 11.84SFRR31 pKa = 11.84GGIRR35 pKa = 11.84FF36 pKa = 3.45

Molecular weight: 4.67 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

8

0

8

1648

31

596

206.0

23.61

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

5.461 ± 1.295

1.335 ± 0.541

6.189 ± 0.75

4.854 ± 1.168

5.825 ± 0.384

6.007 ± 1.045

1.699 ± 0.24

4.126 ± 0.914

5.4 ± 1.179

9.466 ± 1.635

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.184 ± 0.455

4.369 ± 0.829

4.43 ± 1.2

3.823 ± 0.444

7.1 ± 1.521

8.981 ± 0.652

4.248 ± 1.195

7.646 ± 0.377

1.032 ± 0.192

5.825 ± 0.858
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski