Porphyromonas cangingivalis
Average proteome isoelectric point is 6.54
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 1769 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A0A2EWV3|A0A0A2EWV3_PORCN Uncharacterized protein OS=Porphyromonas cangingivalis OX=36874 GN=HQ35_00835 PE=4 SV=1
MM1 pKa = 7.48 EE2 pKa = 5.94 AMDD5 pKa = 4.62 LNEE8 pKa = 3.34 ARR10 pKa = 11.84 IYY12 pKa = 10.4 VGTYY16 pKa = 9.66 AKK18 pKa = 10.48 YY19 pKa = 11.08 NNGSLQGEE27 pKa = 4.35 WVEE30 pKa = 5.59 LSDD33 pKa = 6.54 FYY35 pKa = 11.8 DD36 pKa = 3.73 LDD38 pKa = 3.98 GFMEE42 pKa = 4.67 RR43 pKa = 11.84 CAEE46 pKa = 3.69 IHH48 pKa = 6.37 EE49 pKa = 4.93 DD50 pKa = 3.86 EE51 pKa = 4.98 EE52 pKa = 4.41 EE53 pKa = 4.26 PEE55 pKa = 4.24 YY56 pKa = 10.63 MFQAWEE62 pKa = 4.6 EE63 pKa = 4.3 IPDD66 pKa = 3.74 SLIAEE71 pKa = 4.32 SHH73 pKa = 6.01 LEE75 pKa = 3.81 EE76 pKa = 4.51 TFFEE80 pKa = 5.11 LRR82 pKa = 11.84 DD83 pKa = 3.54 EE84 pKa = 4.57 LDD86 pKa = 3.37 RR87 pKa = 11.84 LNDD90 pKa = 3.71 TEE92 pKa = 5.92 EE93 pKa = 3.68 EE94 pKa = 4.41 AFWVWADD101 pKa = 3.5 GNNAQLTQDD110 pKa = 3.57 AYY112 pKa = 11.64 SLVKK116 pKa = 10.47 SFQSDD121 pKa = 4.36 YY122 pKa = 10.36 IGSYY126 pKa = 10.73 ASRR129 pKa = 11.84 EE130 pKa = 4.07 DD131 pKa = 3.69 FAEE134 pKa = 4.24 EE135 pKa = 3.76 LAKK138 pKa = 10.16 MEE140 pKa = 4.7 NDD142 pKa = 3.78 LPDD145 pKa = 3.5 FALIYY150 pKa = 10.58 FDD152 pKa = 3.68 FGKK155 pKa = 10.95 YY156 pKa = 10.21 ADD158 pKa = 5.5 DD159 pKa = 5.2 LFCTDD164 pKa = 3.63 FWYY167 pKa = 10.77 KK168 pKa = 10.62 DD169 pKa = 3.23 GYY171 pKa = 9.32 VFRR174 pKa = 11.84 NEE176 pKa = 3.68
Molecular weight: 20.87 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.731
IPC2_protein 3.757
IPC_protein 3.745
Toseland 3.541
ProMoST 3.859
Dawson 3.719
Bjellqvist 3.897
Wikipedia 3.617
Rodwell 3.567
Grimsley 3.452
Solomon 3.706
Lehninger 3.656
Nozaki 3.821
DTASelect 3.999
Thurlkill 3.579
EMBOSS 3.63
Sillero 3.859
Patrickios 0.846
IPC_peptide 3.706
IPC2_peptide 3.834
IPC2.peptide.svr19 3.776
Protein with the highest isoelectric point:
>tr|A0A0A2EWI0|A0A0A2EWI0_PORCN Uncharacterized protein OS=Porphyromonas cangingivalis OX=36874 GN=HQ35_00445 PE=4 SV=1
MM1 pKa = 7.07 TNKK4 pKa = 9.43 GRR6 pKa = 11.84 HH7 pKa = 5.2 SPHH10 pKa = 5.96 TNAIISLFNPRR21 pKa = 11.84 NIGYY25 pKa = 8.41 PKK27 pKa = 10.31 EE28 pKa = 4.35 AGLPTRR34 pKa = 11.84 SEE36 pKa = 4.21 VLRR39 pKa = 11.84 LPILFYY45 pKa = 10.4 PKK47 pKa = 9.72 TVASYY52 pKa = 8.41 STMMILRR59 pKa = 11.84 LRR61 pKa = 11.84 GQCRR65 pKa = 11.84 LCTDD69 pKa = 4.58 FPFNPILMRR78 pKa = 11.84 DD79 pKa = 3.24
Molecular weight: 9.08 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.33
IPC2_protein 9.677
IPC_protein 10.452
Toseland 10.35
ProMoST 10.116
Dawson 10.54
Bjellqvist 10.292
Wikipedia 10.76
Rodwell 10.716
Grimsley 10.613
Solomon 10.613
Lehninger 10.584
Nozaki 10.379
DTASelect 10.262
Thurlkill 10.394
EMBOSS 10.76
Sillero 10.467
Patrickios 10.496
IPC_peptide 10.613
IPC2_peptide 9.487
IPC2.peptide.svr19 8.398
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
1769
0
1769
612983
27
1814
346.5
38.95
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
7.205 ± 0.056
0.978 ± 0.02
5.706 ± 0.039
6.736 ± 0.052
4.364 ± 0.039
6.889 ± 0.049
2.147 ± 0.023
7.104 ± 0.047
6.399 ± 0.042
9.6 ± 0.054
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.727 ± 0.022
4.059 ± 0.044
3.941 ± 0.031
3.088 ± 0.027
5.258 ± 0.047
6.499 ± 0.045
5.613 ± 0.032
6.698 ± 0.048
0.986 ± 0.018
4.001 ± 0.04
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here