Brassica yellows virus

Taxonomy: Viruses; Riboviria; Orthornavirae; Pisuviricota; Pisoniviricetes; Sobelivirales; Solemoviridae; Polerovirus; unclassified Polerovirus

Average proteome isoelectric point is 7.44

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 6 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|G3E231|G3E231_9LUTE Movement protein OS=Brassica yellows virus OX=1046403 PE=3 SV=1
MM1 pKa = 7.33NWTNVDD7 pKa = 3.49ARR9 pKa = 11.84WYY11 pKa = 10.53SSNNVKK17 pKa = 10.16AVPMYY22 pKa = 9.63VFPVPEE28 pKa = 4.42GTWSVEE34 pKa = 3.35ISTEE38 pKa = 4.49GYY40 pKa = 10.03QPTASTTDD48 pKa = 3.66PNKK51 pKa = 10.79GKK53 pKa = 10.38VDD55 pKa = 3.46GMIAYY60 pKa = 10.23SDD62 pKa = 3.96DD63 pKa = 3.63QSEE66 pKa = 4.31VWNVGINQNCNITNLKK82 pKa = 9.98ADD84 pKa = 3.77NSWKK88 pKa = 10.43YY89 pKa = 9.19GHH91 pKa = 7.66PDD93 pKa = 3.15MEE95 pKa = 5.12INNCHH100 pKa = 6.29FNQGQVLEE108 pKa = 4.15MDD110 pKa = 4.0GTISFHH116 pKa = 7.99VEE118 pKa = 3.87TTGSDD123 pKa = 2.85ASFFLVGPAVQKK135 pKa = 9.94QSKK138 pKa = 8.52YY139 pKa = 10.45NYY141 pKa = 8.79AVSYY145 pKa = 8.92GAWTDD150 pKa = 3.01RR151 pKa = 11.84DD152 pKa = 3.83MEE154 pKa = 4.95LGLISVSLDD163 pKa = 3.29EE164 pKa = 4.93KK165 pKa = 11.03DD166 pKa = 3.65GSRR169 pKa = 11.84GSAMKK174 pKa = 10.06RR175 pKa = 11.84PRR177 pKa = 11.84RR178 pKa = 11.84EE179 pKa = 3.83GHH181 pKa = 5.89SKK183 pKa = 10.56AVSTWEE189 pKa = 4.21TINLPEE195 pKa = 4.86KK196 pKa = 10.43EE197 pKa = 4.12NSEE200 pKa = 4.44KK201 pKa = 10.99SITSQRR207 pKa = 11.84QDD209 pKa = 3.05SKK211 pKa = 9.87TQPQQGFSDD220 pKa = 4.45AGSEE224 pKa = 5.01DD225 pKa = 3.42NKK227 pKa = 10.88EE228 pKa = 3.64WDD230 pKa = 3.69FTPGTRR236 pKa = 11.84MFLPEE241 pKa = 4.66DD242 pKa = 3.62FEE244 pKa = 4.77EE245 pKa = 4.5PRR247 pKa = 11.84EE248 pKa = 4.15AQNDD252 pKa = 3.6DD253 pKa = 3.66LLRR256 pKa = 11.84DD257 pKa = 3.56RR258 pKa = 11.84GIVVDD263 pKa = 4.82PSLLEE268 pKa = 4.62IPDD271 pKa = 4.19SPPPRR276 pKa = 11.84ADD278 pKa = 4.79HH279 pKa = 7.36KK280 pKa = 11.17DD281 pKa = 3.21TDD283 pKa = 3.24PWAEE287 pKa = 3.88VEE289 pKa = 4.51RR290 pKa = 11.84YY291 pKa = 9.37KK292 pKa = 10.92SSHH295 pKa = 5.98KK296 pKa = 10.91ALVGEE301 pKa = 4.6DD302 pKa = 3.24TRR304 pKa = 11.84SRR306 pKa = 11.84QSSLTGGSLAGGSLRR321 pKa = 11.84KK322 pKa = 9.71LKK324 pKa = 10.41PVTPDD329 pKa = 2.7EE330 pKa = 4.21YY331 pKa = 11.08EE332 pKa = 3.87QAKK335 pKa = 10.71LKK337 pKa = 10.93DD338 pKa = 3.76EE339 pKa = 4.51SRR341 pKa = 11.84LQYY344 pKa = 10.43EE345 pKa = 3.82QDD347 pKa = 3.0LAEE350 pKa = 4.07YY351 pKa = 9.76HH352 pKa = 6.82RR353 pKa = 11.84SLKK356 pKa = 10.47RR357 pKa = 11.84PSSALIPRR365 pKa = 11.84DD366 pKa = 3.71DD367 pKa = 4.31GEE369 pKa = 4.48LMEE372 pKa = 5.15MDD374 pKa = 4.22PSQRR378 pKa = 11.84KK379 pKa = 8.57LMKK382 pKa = 10.37NAGVNLDD389 pKa = 3.31KK390 pKa = 11.01YY391 pKa = 10.8GRR393 pKa = 11.84PIKK396 pKa = 10.51KK397 pKa = 9.98GFLSFRR403 pKa = 11.84KK404 pKa = 9.7

Molecular weight: 45.56 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|G3E226|G3E226_9LUTE Read-through protein OS=Brassica yellows virus OX=1046403 PE=4 SV=1
MM1 pKa = 6.49NTVVGRR7 pKa = 11.84RR8 pKa = 11.84TINGRR13 pKa = 11.84RR14 pKa = 11.84RR15 pKa = 11.84PRR17 pKa = 11.84RR18 pKa = 11.84QTRR21 pKa = 11.84RR22 pKa = 11.84IQRR25 pKa = 11.84NQPVVVVQASRR36 pKa = 11.84TTQRR40 pKa = 11.84RR41 pKa = 11.84PRR43 pKa = 11.84RR44 pKa = 11.84RR45 pKa = 11.84RR46 pKa = 11.84RR47 pKa = 11.84GNNRR51 pKa = 11.84TGRR54 pKa = 11.84TVPTRR59 pKa = 11.84GAGSSEE65 pKa = 3.84TFVFSKK71 pKa = 11.13DD72 pKa = 3.3NLAGSSSGAITFGPSLSDD90 pKa = 4.1CPAFSNGILKK100 pKa = 10.26AYY102 pKa = 10.04HH103 pKa = 6.58EE104 pKa = 4.78YY105 pKa = 10.01KK106 pKa = 10.07ISMVILEE113 pKa = 4.63FVSEE117 pKa = 4.2ASSQNSGSIAYY128 pKa = 9.39EE129 pKa = 3.85LDD131 pKa = 3.29PHH133 pKa = 6.98CKK135 pKa = 10.11LNSLSSTINKK145 pKa = 9.78FGITKK150 pKa = 9.9PGRR153 pKa = 11.84KK154 pKa = 7.21TFTASFINGIEE165 pKa = 3.93WHH167 pKa = 7.14DD168 pKa = 3.64VAEE171 pKa = 4.32DD172 pKa = 3.49QFRR175 pKa = 11.84ILYY178 pKa = 9.14KK179 pKa = 11.0GNGSSSIAGSFRR191 pKa = 11.84ITMKK195 pKa = 10.75CQFHH199 pKa = 6.24NPKK202 pKa = 10.51

Molecular weight: 22.56 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

6

0

6

2664

175

1031

444.0

49.64

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.719 ± 0.652

1.652 ± 0.321

4.73 ± 0.768

6.006 ± 0.498

4.58 ± 0.603

6.532 ± 0.27

2.14 ± 0.345

4.467 ± 0.383

5.818 ± 0.381

9.197 ± 0.852

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.14 ± 0.176

4.429 ± 0.273

5.743 ± 0.281

3.679 ± 0.462

6.194 ± 0.737

10.248 ± 0.838

5.893 ± 0.378

5.33 ± 0.308

1.839 ± 0.234

2.665 ± 0.215
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski