Candidatus Sulfobium mesophilum

Taxonomy: cellular organisms; Bacteria; Nitrospirae; Nitrospira; Nitrospirales; Nitrospiraceae; Candidatus Sulfobium

Average proteome isoelectric point is 7.07

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2739 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A2U3QFM9|A0A2U3QFM9_9BACT Uncharacterized protein OS=Candidatus Sulfobium mesophilum OX=2016548 GN=NBG4_190037 PE=4 SV=1
MM1 pKa = 7.51LKK3 pKa = 10.24AKK5 pKa = 10.3KK6 pKa = 10.36SFIVTLFVALVLVSFGIASAASIGGLSSISGPAPVSDD43 pKa = 4.59SYY45 pKa = 12.08VNPDD49 pKa = 2.97GLGDD53 pKa = 3.65VLLFNYY59 pKa = 10.07YY60 pKa = 9.25NARR63 pKa = 11.84SGMEE67 pKa = 3.81TFFAIVNTDD76 pKa = 3.3SEE78 pKa = 4.1QGTRR82 pKa = 11.84VRR84 pKa = 11.84VRR86 pKa = 11.84FRR88 pKa = 11.84EE89 pKa = 3.96AADD92 pKa = 3.31IANSQCVDD100 pKa = 3.01STTGKK105 pKa = 10.44GATAGSFEE113 pKa = 4.13VLDD116 pKa = 4.22FDD118 pKa = 4.47VCLTAGDD125 pKa = 3.68MWSGFVTAAPGGGAMLCSNDD145 pKa = 3.77VDD147 pKa = 3.8TLIYY151 pKa = 10.92DD152 pKa = 4.39GVNSGLFPAGCVPFKK167 pKa = 11.02SGTFGSATLTADD179 pKa = 3.63DD180 pKa = 3.66TMEE183 pKa = 4.9GYY185 pKa = 10.74FEE187 pKa = 5.18VIGEE191 pKa = 4.2DD192 pKa = 3.38SLNAEE197 pKa = 4.45FTTCKK202 pKa = 10.17PIANFQCTGAGTPDD216 pKa = 3.68ACCTAAGLGTCPDD229 pKa = 3.17QFTDD233 pKa = 3.51VGNEE237 pKa = 3.81LFGNAILIDD246 pKa = 3.99ASTSGSWSYY255 pKa = 11.59DD256 pKa = 2.62ATAIADD262 pKa = 3.75FAFEE266 pKa = 5.06SIQQSPTTSRR276 pKa = 11.84PDD278 pKa = 3.32LASDD282 pKa = 3.87SEE284 pKa = 4.5DD285 pKa = 4.1GIDD288 pKa = 4.21GINYY292 pKa = 9.36ILTKK296 pKa = 9.88EE297 pKa = 4.2HH298 pKa = 6.5VFTIYY303 pKa = 11.08DD304 pKa = 4.76LIHH307 pKa = 6.59ARR309 pKa = 11.84TEE311 pKa = 3.96IVLTEE316 pKa = 3.93PTKK319 pKa = 11.07LLTQQNVGCSLEE331 pKa = 4.05IPGTFNPNIFTDD343 pKa = 4.25DD344 pKa = 3.53SALFTVWNDD353 pKa = 3.2SEE355 pKa = 4.5KK356 pKa = 10.83QLTTVCQFSPCPGGTVNKK374 pKa = 9.94LPHH377 pKa = 6.28EE378 pKa = 4.51VNVLQMNIPTAGVVSDD394 pKa = 4.3ILDD397 pKa = 3.62SAVDD401 pKa = 3.48QVVAITFDD409 pKa = 3.81FGWIDD414 pKa = 3.35INLDD418 pKa = 3.27PDD420 pKa = 3.77VNLTFPHH427 pKa = 5.09QTCFGDD433 pKa = 4.75DD434 pKa = 3.01NVAPFNCSNGWPVLGITLLDD454 pKa = 3.64VDD456 pKa = 5.01HH457 pKa = 6.75GASTGAFPTQYY468 pKa = 9.32FTDD471 pKa = 3.63AFLDD475 pKa = 3.67

Molecular weight: 50.27 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A2U3QKZ1|A0A2U3QKZ1_9BACT Putative streptogramin lyase gluconolactonase family protein OS=Candidatus Sulfobium mesophilum OX=2016548 GN=NBG4_90020 PE=4 SV=1
MM1 pKa = 7.64GKK3 pKa = 10.04NNFWSPYY10 pKa = 7.76IAGIGLGLTLLATFYY25 pKa = 11.05IMGKK29 pKa = 10.24GLGASSAFSIVSAVAVEE46 pKa = 4.44KK47 pKa = 10.73INPEE51 pKa = 3.55FAGNLRR57 pKa = 11.84YY58 pKa = 9.57FSRR61 pKa = 11.84YY62 pKa = 9.64LSTASPLKK70 pKa = 10.21DD71 pKa = 2.99WIVFEE76 pKa = 4.3ILGLFAGALTGALLSRR92 pKa = 11.84NFQIRR97 pKa = 11.84FDD99 pKa = 4.02KK100 pKa = 10.9APGMGGTTRR109 pKa = 11.84LFTAFTGGILIGFAGRR125 pKa = 11.84LARR128 pKa = 11.84GCTSGVALSGGAQLAAAGWIFVISMFISGFLFAAIFRR165 pKa = 11.84RR166 pKa = 11.84LWSS169 pKa = 3.48

Molecular weight: 17.93 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2739

0

2739

780115

22

2513

284.8

31.69

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.979 ± 0.051

1.133 ± 0.022

5.238 ± 0.034

6.732 ± 0.058

4.457 ± 0.039

7.748 ± 0.048

1.894 ± 0.021

7.271 ± 0.04

6.675 ± 0.052

9.684 ± 0.05

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.718 ± 0.022

3.442 ± 0.026

4.089 ± 0.03

2.558 ± 0.026

5.751 ± 0.043

6.387 ± 0.036

5.013 ± 0.036

7.145 ± 0.042

0.977 ± 0.019

3.109 ± 0.026
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski