Falsigemmobacter faecalis 
Average proteome isoelectric point is 6.56 
Get precalculated fractions of proteins 
 
  
    Acidic  
 
  
    pI < 6.8  
 
  
    6.8-7.4  
 
  
    pI > 7.4  
 
  
    Basic  
    
 
  
    All  
 
Note: above files contain also dissociation constants (pKa) 
Virtual 2D-PAGE plot for 3827 proteins (isoelectric point calculated using IPC2_protein) 
 
Get csv file with sequences according to given criteria: 
* You can choose from 21 different methods for calculating isoelectric point 
 Summary statistics related to proteome-wise predictions 
Protein with the lowest isoelectric point: 
>tr|A0A3P3D6U2|A0A3P3D6U2_9RHOB Helix-turn-helix domain-containing protein OS=Falsigemmobacter faecalis OX=2488730 GN=EG244_18675 PE=4 SV=1II1 pKa = 6.3  YY2 pKa = 7.69  TTSQIANNLGTAGDD16 pKa = 3.6  EE17 pKa = 4.23  TEE19 pKa = 4.21  IYY21 pKa = 10.49  FGEE24 pKa = 4.46  FSEE27 pKa = 5.61  AMIGDD32 pKa = 4.12  SQNLSLSVSTDD43 pKa = 2.73  AAYY46 pKa = 11.14  VDD48 pKa = 4.37  GSGNTVSAYY57 pKa = 10.39  QSDD60 pKa = 4.53  LTLMRR65 pKa = 11.84  AISEE69 pKa = 3.98  HH70 pKa = 7.05  DD71 pKa = 3.37  FALEE75 pKa = 3.67  HH76 pKa = 6.9  DD77 pKa = 4.1  VAFAGFNAKK86 pKa = 8.82  GWSLL90 pKa = 3.43  
 9.61 kDa
Isoelectric point according different methods: 
IPC2.protein.svr19  3.756 
IPC2_protein 3.846 
IPC_protein 3.757 
Toseland    3.567 
ProMoST     3.935 
Dawson      3.745 
Bjellqvist  3.91 
Wikipedia   3.694 
Rodwell     3.592 
Grimsley    3.478 
Solomon     3.719 
Lehninger   3.681 
Nozaki      3.884 
DTASelect   4.062 
Thurlkill   3.63 
EMBOSS      3.706 
Sillero     3.872 
Patrickios  0.693 
IPC_peptide 3.719 
IPC2_peptide  3.846 
IPC2.peptide.svr19  3.791 
 Protein with the highest isoelectric point: 
>tr|A0A3P3DT26|A0A3P3DT26_9RHOB UPF0056 membrane protein OS=Falsigemmobacter faecalis OX=2488730 GN=EG244_07905 PE=3 SV=1MM1 pKa = 7.35  PWFWPRR7 pKa = 11.84  SPSRR11 pKa = 11.84  CPAAIKK17 pKa = 10.04  PRR19 pKa = 11.84  FRR21 pKa = 11.84  PRR23 pKa = 11.84  PRR25 pKa = 11.84  RR26 pKa = 11.84  PRR28 pKa = 11.84  PARR31 pKa = 11.84  PHH33 pKa = 6.76  RR34 pKa = 11.84  SASPPPRR41 pKa = 11.84  ARR43 pKa = 11.84  APRR46 pKa = 11.84  PPLTAPARR54 pKa = 11.84  PKK56 pKa = 10.32  KK57 pKa = 9.68  PPPSPPPPPARR68 pKa = 11.84  RR69 pKa = 11.84  SVRR72 pKa = 11.84  SRR74 pKa = 11.84  PRR76 pKa = 11.84  WAIRR80 pKa = 11.84  PNRR83 pKa = 11.84  ASGCARR89 pKa = 11.84  RR90 pKa = 11.84  WSKK93 pKa = 10.98  KK94 pKa = 7.19  SARR97 pKa = 11.84  AWW99 pKa = 3.26  
 11.4 kDa
Isoelectric point according different methods: 
IPC2.protein.svr19  9.49 
IPC2_protein 11.082 
IPC_protein 12.691 
Toseland    12.852 
ProMoST     13.349 
Dawson      12.852 
Bjellqvist  12.852 
Wikipedia   13.32 
Rodwell     12.442 
Grimsley    12.896 
Solomon     13.349 
Lehninger   13.247 
Nozaki      12.852 
DTASelect   12.852 
Thurlkill   12.852 
EMBOSS      13.349 
Sillero     12.852 
Patrickios  12.149 
IPC_peptide 13.349 
IPC2_peptide  12.34 
IPC2.peptide.svr19  9.144 
 Peptides (in silico  digests for buttom-up proteomics) 
Below you can find 
in silico  digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
 
  
    Try  
 
  
    ChTry  
 
  
    ArgC  
 
  
    LysN  
 
  
    TryLysC  
 
  
    Try  
 
  
    ChTry  
 
  
    ArgC  
 
  
    LysN  
 
  
    TryLysC  
 
  
    Try  
 
  
    ChTry  
 
  
    ArgC  
 
  
    LysN  
 
  
    TryLysC  
 
  
    Try  
 
  
    ChTry  
 
  
    ArgC  
 
  
    LysN  
 
  
    TryLysC  
 
  
    Try  
 
  
    ChTry  
 
  
    ArgC  
 
  
    LysN  
 
  
    TryLysC  
   
  
General Statistics 
    
      
        Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
 
     
    
      
        3827 
0
3827 
1200599
26
5765
313.7
33.9
          
     
  
 
    Amino acid frequency 
  
  
    
      
        Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
 
     
    
                 
        12.998 ± 0.063
0.856 ± 0.013
5.292 ± 0.03
5.92 ± 0.035
3.589 ± 0.028
8.954 ± 0.05
1.924 ± 0.019
4.915 ± 0.025
2.903 ± 0.03
10.902 ± 0.046
          
     
  
  
  
      
          
        Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
 
     
    
                 
        2.632 ± 0.02
2.319 ± 0.023
5.368 ± 0.038
3.253 ± 0.023
7.275 ± 0.043
5.433 ± 0.029
5.141 ± 0.029
6.932 ± 0.033
1.391 ± 0.018
2.001 ± 0.018
          
     
  
  
  
Note:  For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein levelMost of the basic statistics you can see at this page can be downloaded from this CSV file  
For dipeptide frequency statistics click here