Shuangao insect virus 11
Average proteome isoelectric point is 6.87
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 1 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A1L3KGB6|A0A1L3KGB6_9VIRU RNA replicase OS=Shuangao insect virus 11 OX=1923467 PE=3 SV=1
MM1 pKa = 7.28 VKK3 pKa = 9.84 IEE5 pKa = 4.79 LDD7 pKa = 3.49 SSSLCEE13 pKa = 4.33 PIVVKK18 pKa = 10.52 SLILGATTTATLYY31 pKa = 9.5 FVWRR35 pKa = 11.84 EE36 pKa = 3.58 RR37 pKa = 11.84 ARR39 pKa = 11.84 IVAAVAIGPYY49 pKa = 8.19 PTALVSPVSRR59 pKa = 11.84 FFQRR63 pKa = 11.84 TLIDD67 pKa = 3.16 RR68 pKa = 11.84 TKK70 pKa = 10.75 RR71 pKa = 11.84 DD72 pKa = 3.34 VSVEE76 pKa = 3.92 WFPHH80 pKa = 5.48 NFTPTTFQRR89 pKa = 11.84 RR90 pKa = 11.84 VTDD93 pKa = 3.31 NGHH96 pKa = 6.9 PISGAVRR103 pKa = 11.84 DD104 pKa = 3.86 AARR107 pKa = 11.84 VLISDD112 pKa = 5.6 AIDD115 pKa = 3.29 STGLKK120 pKa = 9.9 RR121 pKa = 11.84 FEE123 pKa = 4.52 ISPSSRR129 pKa = 11.84 TSAGARR135 pKa = 11.84 CKK137 pKa = 9.27 HH138 pKa = 4.53 QHH140 pKa = 5.27 YY141 pKa = 10.24 AAGDD145 pKa = 3.58 LHH147 pKa = 8.19 LDD149 pKa = 4.26 LSNQIPQTGEE159 pKa = 3.85 VIVCIDD165 pKa = 3.16 TDD167 pKa = 3.94 YY168 pKa = 11.35 YY169 pKa = 11.34 LRR171 pKa = 11.84 DD172 pKa = 3.24 VSEE175 pKa = 4.93 AFGHH179 pKa = 5.08 VNPIIMHH186 pKa = 5.8 TFSPRR191 pKa = 11.84 RR192 pKa = 11.84 VAGLDD197 pKa = 3.18 GDD199 pKa = 4.07 SVYY202 pKa = 10.53 TIKK205 pKa = 10.94 NNQIRR210 pKa = 11.84 YY211 pKa = 7.56 QVSGGAAWSHH221 pKa = 6.11 EE222 pKa = 3.93 VWDD225 pKa = 3.45 WCGYY229 pKa = 10.6 GEE231 pKa = 5.87 FIRR234 pKa = 11.84 LDD236 pKa = 3.5 AYY238 pKa = 11.27 LNSWWSRR245 pKa = 11.84 ALSWFGVRR253 pKa = 11.84 KK254 pKa = 8.29 TILTKK259 pKa = 10.13 VHH261 pKa = 7.36 HH262 pKa = 6.25 IRR264 pKa = 11.84 PWADD268 pKa = 3.16 SPDD271 pKa = 3.77 RR272 pKa = 11.84 ALVWCIPQYY281 pKa = 8.73 TFWRR285 pKa = 11.84 FSFLADD291 pKa = 3.61 DD292 pKa = 3.56 VHH294 pKa = 6.68 ARR296 pKa = 11.84 PLARR300 pKa = 11.84 VVFQDD305 pKa = 3.19 STRR308 pKa = 11.84 PGWNSLVYY316 pKa = 10.5 LDD318 pKa = 5.45 ADD320 pKa = 3.58 NKK322 pKa = 9.46 QWISIGRR329 pKa = 11.84 EE330 pKa = 4.08 GEE332 pKa = 4.07 DD333 pKa = 3.41 ATVEE337 pKa = 4.07 MPKK340 pKa = 9.7 IDD342 pKa = 4.28 FDD344 pKa = 4.27 VLMGLQSSQSVTSRR358 pKa = 11.84 MIGMKK363 pKa = 8.91 YY364 pKa = 9.02 TSPHH368 pKa = 5.5 EE369 pKa = 4.02 LALVGQYY376 pKa = 9.08 YY377 pKa = 10.42 RR378 pKa = 11.84 KK379 pKa = 8.83 GTADD383 pKa = 3.4 TPDD386 pKa = 3.47 SFRR389 pKa = 11.84 LARR392 pKa = 11.84 PASVRR397 pKa = 11.84 VHH399 pKa = 5.28 WPEE402 pKa = 3.84 SVEE405 pKa = 3.91 ADD407 pKa = 3.56 VPEE410 pKa = 4.35 VSSRR414 pKa = 11.84 SYY416 pKa = 11.0 GPPVVTDD423 pKa = 3.62 EE424 pKa = 5.3 NMMPMIKK431 pKa = 9.39 RR432 pKa = 11.84 WEE434 pKa = 4.08 ALSLSLEE441 pKa = 3.88 RR442 pKa = 11.84 RR443 pKa = 11.84 VEE445 pKa = 4.05 FVRR448 pKa = 11.84 NNKK451 pKa = 9.18 IPPKK455 pKa = 9.92 KK456 pKa = 8.03 FQSYY460 pKa = 10.2 AEE462 pKa = 3.98 EE463 pKa = 4.49 FLRR466 pKa = 11.84 CVCPEE471 pKa = 4.06 AGSGVPYY478 pKa = 9.9 TLEE481 pKa = 4.15 DD482 pKa = 3.3 TASFLDD488 pKa = 4.09 KK489 pKa = 10.32 PSQVLAVKK497 pKa = 8.87 QVWEE501 pKa = 4.26 TVDD504 pKa = 3.25 MPVRR508 pKa = 11.84 RR509 pKa = 11.84 LIEE512 pKa = 4.2 CFLKK516 pKa = 10.91 NEE518 pKa = 4.21 PCMKK522 pKa = 9.42 PGRR525 pKa = 11.84 IISAFADD532 pKa = 3.3 ARR534 pKa = 11.84 YY535 pKa = 9.61 LLQLSAFTLAFRR547 pKa = 11.84 DD548 pKa = 3.59 RR549 pKa = 11.84 VLHH552 pKa = 6.57 AEE554 pKa = 4.22 HH555 pKa = 6.78 NEE557 pKa = 3.68 HH558 pKa = 6.72 WFCPGLTPRR567 pKa = 11.84 GIADD571 pKa = 3.74 KK572 pKa = 10.89 VCDD575 pKa = 3.95 YY576 pKa = 11.53 VSMVDD581 pKa = 3.9 QVAEE585 pKa = 4.05 GDD587 pKa = 3.86 YY588 pKa = 11.32 SNLDD592 pKa = 3.29 GTVSVWLQRR601 pKa = 11.84 RR602 pKa = 11.84 VMNAVYY608 pKa = 10.24 LRR610 pKa = 11.84 YY611 pKa = 9.18 FHH613 pKa = 7.07 HH614 pKa = 7.27 SYY616 pKa = 10.57 RR617 pKa = 11.84 KK618 pKa = 9.16 EE619 pKa = 4.07 LQAHH623 pKa = 6.99 LDD625 pKa = 3.67 MLVTCPARR633 pKa = 11.84 AKK635 pKa = 10.48 RR636 pKa = 11.84 FGFAYY641 pKa = 10.41 DD642 pKa = 3.38 AGHH645 pKa = 6.1 GVKK648 pKa = 10.19 SGSPTTCDD656 pKa = 3.1 ANTIAGGFVQYY667 pKa = 10.52 CAVRR671 pKa = 11.84 EE672 pKa = 4.21 TQPEE676 pKa = 4.37 LTPSEE681 pKa = 4.16 AFRR684 pKa = 11.84 AIGLAFGDD692 pKa = 4.31 DD693 pKa = 3.55 GLFDD697 pKa = 3.68 RR698 pKa = 11.84 RR699 pKa = 11.84 YY700 pKa = 9.01 KK701 pKa = 10.77 RR702 pKa = 11.84 GWAKK706 pKa = 10.09 SAEE709 pKa = 4.07 RR710 pKa = 11.84 LGLVLKK716 pKa = 10.49 IEE718 pKa = 4.92 PYY720 pKa = 10.05 QPEE723 pKa = 3.92 QGVCFLARR731 pKa = 11.84 VFPDD735 pKa = 3.21 PWNTNTSFQDD745 pKa = 3.65 PLRR748 pKa = 11.84 TWRR751 pKa = 11.84 KK752 pKa = 8.53 LHH754 pKa = 5.74 LTSRR758 pKa = 11.84 DD759 pKa = 3.42 PTIKK763 pKa = 10.65 LEE765 pKa = 4.12 SAAVDD770 pKa = 3.46 RR771 pKa = 11.84 LEE773 pKa = 5.18 GYY775 pKa = 10.73 LITDD779 pKa = 4.52 PLTPVTSHH787 pKa = 5.62 YY788 pKa = 11.52 ARR790 pKa = 11.84 MILRR794 pKa = 11.84 TFGRR798 pKa = 11.84 GAEE801 pKa = 4.19 SEE803 pKa = 4.51 VVRR806 pKa = 11.84 RR807 pKa = 11.84 SRR809 pKa = 11.84 KK810 pKa = 9.42 SVDD813 pKa = 3.0 RR814 pKa = 11.84 EE815 pKa = 3.7 KK816 pKa = 10.77 PYY818 pKa = 10.24 WLTVGGSWPQNPADD832 pKa = 4.0 VDD834 pKa = 3.93 LMWRR838 pKa = 11.84 VIAEE842 pKa = 3.89 RR843 pKa = 11.84 TGFDD847 pKa = 3.55 DD848 pKa = 4.11 ATLRR852 pKa = 11.84 DD853 pKa = 3.58 YY854 pKa = 11.39 AGRR857 pKa = 11.84 CEE859 pKa = 4.38 TIDD862 pKa = 3.18 NHH864 pKa = 6.25 LAHH867 pKa = 7.02 HH868 pKa = 6.33 SCSLTT873 pKa = 3.34
Molecular weight: 99.1 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 6.869
IPC2_protein 7.132
IPC_protein 7.015
Toseland 6.883
ProMoST 7.556
Dawson 7.658
Bjellqvist 8.039
Wikipedia 7.585
Rodwell 7.673
Grimsley 6.985
Solomon 7.688
Lehninger 7.702
Nozaki 8.244
DTASelect 7.863
Thurlkill 7.907
EMBOSS 7.922
Sillero 8.185
Patrickios 4.304
IPC_peptide 7.688
IPC2_peptide 7.41
IPC2.peptide.svr19 7.125
Protein with the highest isoelectric point:
>tr|A0A1L3KGB6|A0A1L3KGB6_9VIRU RNA replicase OS=Shuangao insect virus 11 OX=1923467 PE=3 SV=1
MM1 pKa = 7.28 VKK3 pKa = 9.84 IEE5 pKa = 4.79 LDD7 pKa = 3.49 SSSLCEE13 pKa = 4.33 PIVVKK18 pKa = 10.52 SLILGATTTATLYY31 pKa = 9.5 FVWRR35 pKa = 11.84 EE36 pKa = 3.58 RR37 pKa = 11.84 ARR39 pKa = 11.84 IVAAVAIGPYY49 pKa = 8.19 PTALVSPVSRR59 pKa = 11.84 FFQRR63 pKa = 11.84 TLIDD67 pKa = 3.16 RR68 pKa = 11.84 TKK70 pKa = 10.75 RR71 pKa = 11.84 DD72 pKa = 3.34 VSVEE76 pKa = 3.92 WFPHH80 pKa = 5.48 NFTPTTFQRR89 pKa = 11.84 RR90 pKa = 11.84 VTDD93 pKa = 3.31 NGHH96 pKa = 6.9 PISGAVRR103 pKa = 11.84 DD104 pKa = 3.86 AARR107 pKa = 11.84 VLISDD112 pKa = 5.6 AIDD115 pKa = 3.29 STGLKK120 pKa = 9.9 RR121 pKa = 11.84 FEE123 pKa = 4.52 ISPSSRR129 pKa = 11.84 TSAGARR135 pKa = 11.84 CKK137 pKa = 9.27 HH138 pKa = 4.53 QHH140 pKa = 5.27 YY141 pKa = 10.24 AAGDD145 pKa = 3.58 LHH147 pKa = 8.19 LDD149 pKa = 4.26 LSNQIPQTGEE159 pKa = 3.85 VIVCIDD165 pKa = 3.16 TDD167 pKa = 3.94 YY168 pKa = 11.35 YY169 pKa = 11.34 LRR171 pKa = 11.84 DD172 pKa = 3.24 VSEE175 pKa = 4.93 AFGHH179 pKa = 5.08 VNPIIMHH186 pKa = 5.8 TFSPRR191 pKa = 11.84 RR192 pKa = 11.84 VAGLDD197 pKa = 3.18 GDD199 pKa = 4.07 SVYY202 pKa = 10.53 TIKK205 pKa = 10.94 NNQIRR210 pKa = 11.84 YY211 pKa = 7.56 QVSGGAAWSHH221 pKa = 6.11 EE222 pKa = 3.93 VWDD225 pKa = 3.45 WCGYY229 pKa = 10.6 GEE231 pKa = 5.87 FIRR234 pKa = 11.84 LDD236 pKa = 3.5 AYY238 pKa = 11.27 LNSWWSRR245 pKa = 11.84 ALSWFGVRR253 pKa = 11.84 KK254 pKa = 8.29 TILTKK259 pKa = 10.13 VHH261 pKa = 7.36 HH262 pKa = 6.25 IRR264 pKa = 11.84 PWADD268 pKa = 3.16 SPDD271 pKa = 3.77 RR272 pKa = 11.84 ALVWCIPQYY281 pKa = 8.73 TFWRR285 pKa = 11.84 FSFLADD291 pKa = 3.61 DD292 pKa = 3.56 VHH294 pKa = 6.68 ARR296 pKa = 11.84 PLARR300 pKa = 11.84 VVFQDD305 pKa = 3.19 STRR308 pKa = 11.84 PGWNSLVYY316 pKa = 10.5 LDD318 pKa = 5.45 ADD320 pKa = 3.58 NKK322 pKa = 9.46 QWISIGRR329 pKa = 11.84 EE330 pKa = 4.08 GEE332 pKa = 4.07 DD333 pKa = 3.41 ATVEE337 pKa = 4.07 MPKK340 pKa = 9.7 IDD342 pKa = 4.28 FDD344 pKa = 4.27 VLMGLQSSQSVTSRR358 pKa = 11.84 MIGMKK363 pKa = 8.91 YY364 pKa = 9.02 TSPHH368 pKa = 5.5 EE369 pKa = 4.02 LALVGQYY376 pKa = 9.08 YY377 pKa = 10.42 RR378 pKa = 11.84 KK379 pKa = 8.83 GTADD383 pKa = 3.4 TPDD386 pKa = 3.47 SFRR389 pKa = 11.84 LARR392 pKa = 11.84 PASVRR397 pKa = 11.84 VHH399 pKa = 5.28 WPEE402 pKa = 3.84 SVEE405 pKa = 3.91 ADD407 pKa = 3.56 VPEE410 pKa = 4.35 VSSRR414 pKa = 11.84 SYY416 pKa = 11.0 GPPVVTDD423 pKa = 3.62 EE424 pKa = 5.3 NMMPMIKK431 pKa = 9.39 RR432 pKa = 11.84 WEE434 pKa = 4.08 ALSLSLEE441 pKa = 3.88 RR442 pKa = 11.84 RR443 pKa = 11.84 VEE445 pKa = 4.05 FVRR448 pKa = 11.84 NNKK451 pKa = 9.18 IPPKK455 pKa = 9.92 KK456 pKa = 8.03 FQSYY460 pKa = 10.2 AEE462 pKa = 3.98 EE463 pKa = 4.49 FLRR466 pKa = 11.84 CVCPEE471 pKa = 4.06 AGSGVPYY478 pKa = 9.9 TLEE481 pKa = 4.15 DD482 pKa = 3.3 TASFLDD488 pKa = 4.09 KK489 pKa = 10.32 PSQVLAVKK497 pKa = 8.87 QVWEE501 pKa = 4.26 TVDD504 pKa = 3.25 MPVRR508 pKa = 11.84 RR509 pKa = 11.84 LIEE512 pKa = 4.2 CFLKK516 pKa = 10.91 NEE518 pKa = 4.21 PCMKK522 pKa = 9.42 PGRR525 pKa = 11.84 IISAFADD532 pKa = 3.3 ARR534 pKa = 11.84 YY535 pKa = 9.61 LLQLSAFTLAFRR547 pKa = 11.84 DD548 pKa = 3.59 RR549 pKa = 11.84 VLHH552 pKa = 6.57 AEE554 pKa = 4.22 HH555 pKa = 6.78 NEE557 pKa = 3.68 HH558 pKa = 6.72 WFCPGLTPRR567 pKa = 11.84 GIADD571 pKa = 3.74 KK572 pKa = 10.89 VCDD575 pKa = 3.95 YY576 pKa = 11.53 VSMVDD581 pKa = 3.9 QVAEE585 pKa = 4.05 GDD587 pKa = 3.86 YY588 pKa = 11.32 SNLDD592 pKa = 3.29 GTVSVWLQRR601 pKa = 11.84 RR602 pKa = 11.84 VMNAVYY608 pKa = 10.24 LRR610 pKa = 11.84 YY611 pKa = 9.18 FHH613 pKa = 7.07 HH614 pKa = 7.27 SYY616 pKa = 10.57 RR617 pKa = 11.84 KK618 pKa = 9.16 EE619 pKa = 4.07 LQAHH623 pKa = 6.99 LDD625 pKa = 3.67 MLVTCPARR633 pKa = 11.84 AKK635 pKa = 10.48 RR636 pKa = 11.84 FGFAYY641 pKa = 10.41 DD642 pKa = 3.38 AGHH645 pKa = 6.1 GVKK648 pKa = 10.19 SGSPTTCDD656 pKa = 3.1 ANTIAGGFVQYY667 pKa = 10.52 CAVRR671 pKa = 11.84 EE672 pKa = 4.21 TQPEE676 pKa = 4.37 LTPSEE681 pKa = 4.16 AFRR684 pKa = 11.84 AIGLAFGDD692 pKa = 4.31 DD693 pKa = 3.55 GLFDD697 pKa = 3.68 RR698 pKa = 11.84 RR699 pKa = 11.84 YY700 pKa = 9.01 KK701 pKa = 10.77 RR702 pKa = 11.84 GWAKK706 pKa = 10.09 SAEE709 pKa = 4.07 RR710 pKa = 11.84 LGLVLKK716 pKa = 10.49 IEE718 pKa = 4.92 PYY720 pKa = 10.05 QPEE723 pKa = 3.92 QGVCFLARR731 pKa = 11.84 VFPDD735 pKa = 3.21 PWNTNTSFQDD745 pKa = 3.65 PLRR748 pKa = 11.84 TWRR751 pKa = 11.84 KK752 pKa = 8.53 LHH754 pKa = 5.74 LTSRR758 pKa = 11.84 DD759 pKa = 3.42 PTIKK763 pKa = 10.65 LEE765 pKa = 4.12 SAAVDD770 pKa = 3.46 RR771 pKa = 11.84 LEE773 pKa = 5.18 GYY775 pKa = 10.73 LITDD779 pKa = 4.52 PLTPVTSHH787 pKa = 5.62 YY788 pKa = 11.52 ARR790 pKa = 11.84 MILRR794 pKa = 11.84 TFGRR798 pKa = 11.84 GAEE801 pKa = 4.19 SEE803 pKa = 4.51 VVRR806 pKa = 11.84 RR807 pKa = 11.84 SRR809 pKa = 11.84 KK810 pKa = 9.42 SVDD813 pKa = 3.0 RR814 pKa = 11.84 EE815 pKa = 3.7 KK816 pKa = 10.77 PYY818 pKa = 10.24 WLTVGGSWPQNPADD832 pKa = 4.0 VDD834 pKa = 3.93 LMWRR838 pKa = 11.84 VIAEE842 pKa = 3.89 RR843 pKa = 11.84 TGFDD847 pKa = 3.55 DD848 pKa = 4.11 ATLRR852 pKa = 11.84 DD853 pKa = 3.58 YY854 pKa = 11.39 AGRR857 pKa = 11.84 CEE859 pKa = 4.38 TIDD862 pKa = 3.18 NHH864 pKa = 6.25 LAHH867 pKa = 7.02 HH868 pKa = 6.33 SCSLTT873 pKa = 3.34
Molecular weight: 99.1 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 6.869
IPC2_protein 7.132
IPC_protein 7.015
Toseland 6.883
ProMoST 7.556
Dawson 7.658
Bjellqvist 8.039
Wikipedia 7.585
Rodwell 7.673
Grimsley 6.985
Solomon 7.688
Lehninger 7.702
Nozaki 8.244
DTASelect 7.863
Thurlkill 7.907
EMBOSS 7.922
Sillero 8.185
Patrickios 4.304
IPC_peptide 7.688
IPC2_peptide 7.41
IPC2.peptide.svr19 7.125
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
1
0
1
873
873
873
873.0
99.1
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
8.018 ± 0.0
1.947 ± 0.0
6.644 ± 0.0
5.04 ± 0.0
4.353 ± 0.0
5.727 ± 0.0
2.864 ± 0.0
4.467 ± 0.0
3.551 ± 0.0
7.904 ± 0.0
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
1.833 ± 0.0
2.405 ± 0.0
5.727 ± 0.0
2.978 ± 0.0
8.477 ± 0.0
7.446 ± 0.0
6.071 ± 0.0
8.247 ± 0.0
2.749 ± 0.0
3.551 ± 0.0
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here