Streptococcus phage CHPC1027
Average proteome isoelectric point is 6.65
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 46 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A3G8F993|A0A3G8F993_9CAUD Uncharacterized protein OS=Streptococcus phage CHPC1027 OX=2365008 GN=CHPC1027_0040 PE=4 SV=1
MM1 pKa = 7.12 VNWVDD6 pKa = 4.64 KK7 pKa = 10.89 DD8 pKa = 3.93 GNDD11 pKa = 4.47 IPDD14 pKa = 4.25 GDD16 pKa = 4.34 SKK18 pKa = 11.51 DD19 pKa = 3.95 FKK21 pKa = 10.71 PGLFFSFAGDD31 pKa = 3.46 EE32 pKa = 4.32 VNITDD37 pKa = 3.77 TGEE40 pKa = 3.65 GGYY43 pKa = 10.63 YY44 pKa = 9.66 GGYY47 pKa = 8.9 YY48 pKa = 9.56 FRR50 pKa = 11.84 KK51 pKa = 9.96 FEE53 pKa = 4.13 FGQFGTVWLSCWNKK67 pKa = 10.66 DD68 pKa = 3.89 DD69 pKa = 5.14 LVNYY73 pKa = 7.38 YY74 pKa = 8.26 QQ75 pKa = 4.22
Molecular weight: 8.65 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.771
IPC2_protein 3.986
IPC_protein 3.935
Toseland 3.706
ProMoST 4.126
Dawson 3.948
Bjellqvist 4.101
Wikipedia 3.923
Rodwell 3.757
Grimsley 3.617
Solomon 3.923
Lehninger 3.884
Nozaki 4.075
DTASelect 4.342
Thurlkill 3.783
EMBOSS 3.923
Sillero 4.062
Patrickios 1.952
IPC_peptide 3.923
IPC2_peptide 4.024
IPC2.peptide.svr19 3.95
Protein with the highest isoelectric point:
>tr|A0A3G8F965|A0A3G8F965_9CAUD Tail assembly protein OS=Streptococcus phage CHPC1027 OX=2365008 GN=CHPC1027_0010 PE=4 SV=1
MM1 pKa = 7.56 SKK3 pKa = 9.22 TKK5 pKa = 10.5 RR6 pKa = 11.84 GVCANCHH13 pKa = 4.61 TVFEE17 pKa = 4.52 VSKK20 pKa = 9.76 KK21 pKa = 7.91 QRR23 pKa = 11.84 YY24 pKa = 8.42 KK25 pKa = 10.36 IKK27 pKa = 9.76 NGKK30 pKa = 8.84 SVFCSQTCSLEE41 pKa = 4.28 KK42 pKa = 10.59 YY43 pKa = 9.48 GKK45 pKa = 8.13 TKK47 pKa = 9.25 ITISEE52 pKa = 4.39 IPCCRR57 pKa = 11.84 CGKK60 pKa = 9.28 MFTPTYY66 pKa = 9.9 NQYY69 pKa = 10.78 KK70 pKa = 8.85 RR71 pKa = 11.84 YY72 pKa = 9.81 KK73 pKa = 9.02 YY74 pKa = 10.59 NDD76 pKa = 3.28 YY77 pKa = 10.92 VSNSFCSNEE86 pKa = 3.99 CRR88 pKa = 11.84 WKK90 pKa = 10.73 KK91 pKa = 10.23 EE92 pKa = 4.07 YY93 pKa = 9.51 PCKK96 pKa = 10.6 YY97 pKa = 8.82 HH98 pKa = 7.57 DD99 pKa = 5.03 DD100 pKa = 4.04 YY101 pKa = 12.18 VSVFVNGKK109 pKa = 9.91 EE110 pKa = 3.7 ILLDD114 pKa = 3.54 FDD116 pKa = 4.4 VFEE119 pKa = 5.59 KK120 pKa = 9.61 YY121 pKa = 9.3 TNTIYY126 pKa = 10.8 VRR128 pKa = 11.84 NDD130 pKa = 3.13 KK131 pKa = 10.5 ISNYY135 pKa = 8.5 HH136 pKa = 4.86 SVYY139 pKa = 10.59 VFEE142 pKa = 4.99 KK143 pKa = 9.91 GKK145 pKa = 10.6 KK146 pKa = 7.7 VLSRR150 pKa = 11.84 IIMSVTDD157 pKa = 3.35 KK158 pKa = 11.25 NKK160 pKa = 10.42 YY161 pKa = 8.48 VDD163 pKa = 4.77 HH164 pKa = 7.19 INGNPLDD171 pKa = 3.85 NRR173 pKa = 11.84 RR174 pKa = 11.84 SNLRR178 pKa = 11.84 VVSHH182 pKa = 5.96 QEE184 pKa = 3.05 NMMNKK189 pKa = 8.25 KK190 pKa = 8.41 TYY192 pKa = 10.6 KK193 pKa = 10.75 NNTSKK198 pKa = 10.74 IKK200 pKa = 10.53 GVNLNKK206 pKa = 10.19 KK207 pKa = 8.12 GLWVARR213 pKa = 11.84 IQVRR217 pKa = 11.84 NKK219 pKa = 10.32 RR220 pKa = 11.84 IFLGSSKK227 pKa = 10.66 DD228 pKa = 3.3 KK229 pKa = 11.21 SVAEE233 pKa = 4.08 KK234 pKa = 10.91 LRR236 pKa = 11.84 VEE238 pKa = 4.09 AEE240 pKa = 3.59 KK241 pKa = 10.69 KK242 pKa = 10.7 YY243 pKa = 10.41 FGKK246 pKa = 10.57 YY247 pKa = 8.25 DD248 pKa = 3.53 RR249 pKa = 11.84 KK250 pKa = 10.2 YY251 pKa = 11.25 LKK253 pKa = 10.64
Molecular weight: 29.92 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.116
IPC2_protein 9.238
IPC_protein 9.107
Toseland 9.882
ProMoST 9.589
Dawson 10.101
Bjellqvist 9.78
Wikipedia 10.262
Rodwell 10.613
Grimsley 10.175
Solomon 10.116
Lehninger 10.087
Nozaki 9.94
DTASelect 9.75
Thurlkill 9.955
EMBOSS 10.292
Sillero 10.028
Patrickios 9.955
IPC_peptide 10.116
IPC2_peptide 8.478
IPC2.peptide.svr19 8.089
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
46
0
46
11092
38
1593
241.1
27.32
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
6.248 ± 0.526
0.622 ± 0.164
6.626 ± 0.232
6.428 ± 0.451
4.354 ± 0.215
6.843 ± 0.501
1.497 ± 0.122
6.536 ± 0.264
8.547 ± 0.5
7.871 ± 0.371
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.236 ± 0.142
6.464 ± 0.247
3.11 ± 0.183
4.093 ± 0.267
4.309 ± 0.31
6.239 ± 0.258
6.212 ± 0.342
6.221 ± 0.318
1.551 ± 0.15
3.994 ± 0.307
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here