Zygosaccharomyces bailii virus Z
Average proteome isoelectric point is 7.34
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 2 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|Q9J0H5|Q9J0H5_9VIRU Major capsid protein OS=Zygosaccharomyces bailii virus Z OX=114871 GN=CAP PE=4 SV=1
MM1 pKa = 7.52 EE2 pKa = 6.26 KK3 pKa = 10.3 DD4 pKa = 3.74 GKK6 pKa = 10.83 SSTWSSFSTALSEE19 pKa = 4.18 IQKK22 pKa = 9.92 EE23 pKa = 4.16 RR24 pKa = 11.84 VPGLEE29 pKa = 4.2 EE30 pKa = 3.61 KK31 pKa = 10.3 TSIDD35 pKa = 3.65 FEE37 pKa = 4.86 VDD39 pKa = 2.78 GKK41 pKa = 10.88 RR42 pKa = 11.84 YY43 pKa = 10.44 SLVSSRR49 pKa = 11.84 KK50 pKa = 7.12 PTDD53 pKa = 3.19 RR54 pKa = 11.84 EE55 pKa = 4.14 VEE57 pKa = 4.27 LKK59 pKa = 10.85 SLIPSAILTEE69 pKa = 3.86 QLLSNEE75 pKa = 4.62 HH76 pKa = 5.66 IGKK79 pKa = 9.16 YY80 pKa = 7.77 FEE82 pKa = 5.29 RR83 pKa = 11.84 IRR85 pKa = 11.84 KK86 pKa = 8.68 ALIVKK91 pKa = 7.61 NTQADD96 pKa = 4.03 LVEE99 pKa = 4.58 LLNSFTTVLTNIHH112 pKa = 5.73 QVSLYY117 pKa = 10.07 IEE119 pKa = 4.22 HH120 pKa = 7.08 HH121 pKa = 7.16 PDD123 pKa = 2.76 TTAVAKK129 pKa = 10.8 SFEE132 pKa = 4.15 NYY134 pKa = 9.55 KK135 pKa = 10.5 LKK137 pKa = 10.72 KK138 pKa = 10.23 DD139 pKa = 3.6 LSKK142 pKa = 10.84 WKK144 pKa = 10.6 LDD146 pKa = 3.5 MLNSLSTVDD155 pKa = 3.69 GRR157 pKa = 11.84 VQIHH161 pKa = 5.45 VNEE164 pKa = 4.04 ILAMFHH170 pKa = 5.83 GVKK173 pKa = 10.35 NAAQKK178 pKa = 9.8 DD179 pKa = 3.73 AQMKK183 pKa = 8.82 EE184 pKa = 3.74 EE185 pKa = 4.17 KK186 pKa = 10.08 ARR188 pKa = 11.84 KK189 pKa = 9.1 LMKK192 pKa = 10.26 QQVEE196 pKa = 4.02 LARR199 pKa = 11.84 QEE201 pKa = 3.82 FEE203 pKa = 3.98 EE204 pKa = 4.77 RR205 pKa = 11.84 IKK207 pKa = 10.65 HH208 pKa = 4.57 VRR210 pKa = 11.84 PFQGLMLLTGSFKK223 pKa = 10.06 EE224 pKa = 4.09 QSDD227 pKa = 3.98 RR228 pKa = 11.84 VHH230 pKa = 6.51 EE231 pKa = 4.2 MARR234 pKa = 11.84 QEE236 pKa = 4.26 WMSLPDD242 pKa = 3.62 TEE244 pKa = 5.47 KK245 pKa = 11.18 SSDD248 pKa = 3.25 YY249 pKa = 11.02 DD250 pKa = 2.99 AWAQAWWATRR260 pKa = 11.84 KK261 pKa = 10.32 DD262 pKa = 3.67 GEE264 pKa = 4.23 IFAVLEE270 pKa = 4.27 STVMTEE276 pKa = 3.66 EE277 pKa = 3.76 FLEE280 pKa = 4.71 FFSQHH285 pKa = 4.2 WLFRR289 pKa = 11.84 GG290 pKa = 3.45
Molecular weight: 33.68 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 6.064
IPC2_protein 5.881
IPC_protein 5.931
Toseland 6.465
ProMoST 6.275
Dawson 6.186
Bjellqvist 6.186
Wikipedia 6.198
Rodwell 6.173
Grimsley 6.693
Solomon 6.186
Lehninger 6.186
Nozaki 6.44
DTASelect 6.634
Thurlkill 6.649
EMBOSS 6.62
Sillero 6.547
Patrickios 4.813
IPC_peptide 6.198
IPC2_peptide 6.491
IPC2.peptide.svr19 6.564
Protein with the highest isoelectric point:
>tr|Q9J0H5|Q9J0H5_9VIRU Major capsid protein OS=Zygosaccharomyces bailii virus Z OX=114871 GN=CAP PE=4 SV=1
MM1 pKa = 7.52 EE2 pKa = 6.26 KK3 pKa = 10.3 DD4 pKa = 3.74 GKK6 pKa = 10.83 SSTWSSFSTALSEE19 pKa = 4.18 IQKK22 pKa = 9.92 EE23 pKa = 4.16 RR24 pKa = 11.84 VPGLEE29 pKa = 4.2 EE30 pKa = 3.61 KK31 pKa = 10.3 TSIDD35 pKa = 3.65 FEE37 pKa = 4.86 VDD39 pKa = 2.78 GKK41 pKa = 10.88 RR42 pKa = 11.84 YY43 pKa = 10.44 SLVSSRR49 pKa = 11.84 KK50 pKa = 7.12 PTDD53 pKa = 3.19 RR54 pKa = 11.84 EE55 pKa = 4.14 VEE57 pKa = 4.27 LKK59 pKa = 10.85 SLIPSAILTEE69 pKa = 3.86 QLLSNEE75 pKa = 4.62 HH76 pKa = 5.66 IGKK79 pKa = 9.16 YY80 pKa = 7.77 FEE82 pKa = 5.29 RR83 pKa = 11.84 IRR85 pKa = 11.84 KK86 pKa = 8.68 ALIVKK91 pKa = 7.61 NTQADD96 pKa = 4.03 LVEE99 pKa = 4.58 LLNSFTTVLTNIHH112 pKa = 5.73 QVSLYY117 pKa = 10.07 IEE119 pKa = 4.22 HH120 pKa = 7.08 HH121 pKa = 7.16 PDD123 pKa = 2.76 TTAVAKK129 pKa = 10.8 SFEE132 pKa = 4.15 NYY134 pKa = 9.55 KK135 pKa = 10.5 LKK137 pKa = 10.72 KK138 pKa = 10.23 DD139 pKa = 3.6 LSKK142 pKa = 10.84 WKK144 pKa = 10.6 LDD146 pKa = 3.5 MLNSLSTVDD155 pKa = 3.69 GRR157 pKa = 11.84 VQIHH161 pKa = 5.45 VNEE164 pKa = 4.04 ILAMFHH170 pKa = 5.83 GVKK173 pKa = 10.35 NAAQKK178 pKa = 9.8 DD179 pKa = 3.73 AQMKK183 pKa = 8.82 EE184 pKa = 3.74 EE185 pKa = 4.17 KK186 pKa = 10.08 ARR188 pKa = 11.84 KK189 pKa = 9.1 LMKK192 pKa = 10.26 QQVEE196 pKa = 4.02 LARR199 pKa = 11.84 QEE201 pKa = 3.82 FEE203 pKa = 3.98 EE204 pKa = 4.77 RR205 pKa = 11.84 IKK207 pKa = 10.65 HH208 pKa = 4.57 VRR210 pKa = 11.84 PFQGLMLLTGSFKK223 pKa = 10.06 EE224 pKa = 4.09 QSDD227 pKa = 3.98 RR228 pKa = 11.84 VHH230 pKa = 6.51 EE231 pKa = 4.2 MARR234 pKa = 11.84 QEE236 pKa = 4.26 WMSLPDD242 pKa = 3.62 TEE244 pKa = 5.47 KK245 pKa = 11.18 SSDD248 pKa = 3.25 YY249 pKa = 11.02 DD250 pKa = 2.99 AWAQAWWATRR260 pKa = 11.84 KK261 pKa = 10.32 DD262 pKa = 3.67 GEE264 pKa = 4.23 IFAVLEE270 pKa = 4.27 STVMTEE276 pKa = 3.66 EE277 pKa = 3.76 FLEE280 pKa = 4.71 FFSQHH285 pKa = 5.1 WLFEE289 pKa = 4.61 ADD291 pKa = 3.21 RR292 pKa = 11.84 LTWSRR297 pKa = 11.84 RR298 pKa = 11.84 FKK300 pKa = 10.88 QSVSRR305 pKa = 11.84 YY306 pKa = 8.41 GYY308 pKa = 10.8 GFNKK312 pKa = 9.52 EE313 pKa = 4.08 KK314 pKa = 10.87 KK315 pKa = 9.45 IDD317 pKa = 3.73 TKK319 pKa = 10.98 FKK321 pKa = 10.65 NLKK324 pKa = 9.76 KK325 pKa = 10.23 INNIQKK331 pKa = 9.94 HH332 pKa = 5.56 PPLVGRR338 pKa = 11.84 GPCQRR343 pKa = 11.84 NLFFEE348 pKa = 4.14 NTLRR352 pKa = 11.84 SMGGSEE358 pKa = 4.08 VRR360 pKa = 11.84 AIDD363 pKa = 3.83 LYY365 pKa = 11.35 KK366 pKa = 10.83 NYY368 pKa = 10.27 GRR370 pKa = 11.84 FGGSRR375 pKa = 11.84 VDD377 pKa = 3.94 SILYY381 pKa = 7.91 MKK383 pKa = 10.7 NFDD386 pKa = 4.0 KK387 pKa = 10.8 KK388 pKa = 11.27 YY389 pKa = 7.93 PTLDD393 pKa = 4.63 LDD395 pKa = 3.59 WKK397 pKa = 10.84 SNKK400 pKa = 8.0 EE401 pKa = 3.51 WCFEE405 pKa = 3.68 IFGKK409 pKa = 10.6 VNHH412 pKa = 6.75 IKK414 pKa = 10.64 LFDD417 pKa = 3.54 MEE419 pKa = 4.54 YY420 pKa = 10.6 CLDD423 pKa = 3.94 VPDD426 pKa = 4.72 VPSDD430 pKa = 3.46 TSPGITYY437 pKa = 10.15 KK438 pKa = 10.85 RR439 pKa = 11.84 LGFIKK444 pKa = 10.38 KK445 pKa = 10.01 ADD447 pKa = 4.0 ALPVCLSHH455 pKa = 7.82 LRR457 pKa = 11.84 DD458 pKa = 3.51 VLKK461 pKa = 10.71 KK462 pKa = 9.77 IEE464 pKa = 3.98 KK465 pKa = 10.22 HH466 pKa = 5.19 EE467 pKa = 4.16 LQTLPEE473 pKa = 4.32 IAWCIAGRR481 pKa = 11.84 PKK483 pKa = 9.89 FTEE486 pKa = 4.05 LNKK489 pKa = 10.36 AFQKK493 pKa = 10.88 VYY495 pKa = 10.67 DD496 pKa = 3.92 RR497 pKa = 11.84 QSVGRR502 pKa = 11.84 SIWVSDD508 pKa = 3.61 MEE510 pKa = 4.1 EE511 pKa = 4.75 AILSRR516 pKa = 11.84 HH517 pKa = 4.44 FTRR520 pKa = 11.84 YY521 pKa = 8.27 IDD523 pKa = 3.7 KK524 pKa = 10.66 NITGTPTFHH533 pKa = 7.85 KK534 pKa = 10.65 IMINFDD540 pKa = 3.25 KK541 pKa = 11.43 VKK543 pKa = 10.36 DD544 pKa = 3.95 AKK546 pKa = 10.65 HH547 pKa = 4.89 LQNWVEE553 pKa = 3.92 QYY555 pKa = 11.46 DD556 pKa = 4.08 YY557 pKa = 10.66 IIEE560 pKa = 4.4 ADD562 pKa = 3.55 YY563 pKa = 11.62 SKK565 pKa = 10.99 FDD567 pKa = 3.57 SSVPACVVRR576 pKa = 11.84 KK577 pKa = 9.74 AFQIFKK583 pKa = 10.04 EE584 pKa = 4.17 LFVSFDD590 pKa = 3.07 HH591 pKa = 6.12 TTATVYY597 pKa = 11.0 DD598 pKa = 3.9 FLIEE602 pKa = 4.03 NFLNSKK608 pKa = 10.18 VDD610 pKa = 3.79 LSDD613 pKa = 3.63 GRR615 pKa = 11.84 LLQKK619 pKa = 10.77 FGGVPSGSGFTSIIGSICNYY639 pKa = 10.04 IMLDD643 pKa = 3.41 TCFMKK648 pKa = 10.21 MGLDD652 pKa = 3.48 KK653 pKa = 11.16 NRR655 pKa = 11.84 WSAIVYY661 pKa = 10.6 GDD663 pKa = 4.04 DD664 pKa = 3.76 CLIGIKK670 pKa = 10.25 KK671 pKa = 9.35 PLHH674 pKa = 5.47 KK675 pKa = 10.45 HH676 pKa = 5.21 EE677 pKa = 5.7 DD678 pKa = 3.45 SAFKK682 pKa = 10.21 IRR684 pKa = 11.84 KK685 pKa = 9.0 LMLKK689 pKa = 10.26 FMQLLFDD696 pKa = 3.71 ITLDD700 pKa = 3.78 PKK702 pKa = 10.0 DD703 pKa = 3.54 TKK705 pKa = 10.68 LVCEE709 pKa = 4.85 KK710 pKa = 9.5 YY711 pKa = 10.14 VSVIIPEE718 pKa = 4.05 YY719 pKa = 11.15 DD720 pKa = 3.39 EE721 pKa = 5.15 DD722 pKa = 4.34 TTNGTSLLKK731 pKa = 10.05 PSKK734 pKa = 8.66 ITRR737 pKa = 11.84 IEE739 pKa = 4.07 KK740 pKa = 10.74 EE741 pKa = 3.62 PDD743 pKa = 2.95 TFLLSNSSSHH753 pKa = 5.68 RR754 pKa = 11.84 WWYY757 pKa = 10.93 SFEE760 pKa = 4.23 KK761 pKa = 8.55 TWKK764 pKa = 9.59 FLGYY768 pKa = 10.69 SLTCDD773 pKa = 3.01 GRR775 pKa = 11.84 LIRR778 pKa = 11.84 PTRR781 pKa = 11.84 DD782 pKa = 2.81 VLARR786 pKa = 11.84 IYY788 pKa = 10.94 NPEE791 pKa = 4.13 KK792 pKa = 10.17 PVKK795 pKa = 10.22 SWDD798 pKa = 3.37 EE799 pKa = 3.98 HH800 pKa = 4.38 VTLLKK805 pKa = 9.61 MAYY808 pKa = 10.11 LEE810 pKa = 4.33 NYY812 pKa = 8.58 EE813 pKa = 4.23 NAHH816 pKa = 4.84 TRR818 pKa = 11.84 NRR820 pKa = 11.84 IYY822 pKa = 10.41 HH823 pKa = 5.81 YY824 pKa = 11.19 LMDD827 pKa = 4.38 AWWVIHH833 pKa = 6.27 HH834 pKa = 6.84 GCPQRR839 pKa = 11.84 ALPDD843 pKa = 3.0 VDD845 pKa = 3.64 YY846 pKa = 9.95 TVSKK850 pKa = 10.31 GRR852 pKa = 11.84 CFHH855 pKa = 7.43 RR856 pKa = 11.84 YY857 pKa = 5.75 TNHH860 pKa = 5.42 YY861 pKa = 9.12 VHH863 pKa = 6.76 LRR865 pKa = 11.84 WEE867 pKa = 4.13 PSLQGFHH874 pKa = 6.82 NFFDD878 pKa = 3.91 NFDD881 pKa = 4.01 FNMLKK886 pKa = 10.5 LHH888 pKa = 6.24 IQVSLNEE895 pKa = 4.35 FYY897 pKa = 10.96 YY898 pKa = 11.0 SRR900 pKa = 11.84 VKK902 pKa = 10.51 RR903 pKa = 11.84 GRR905 pKa = 11.84 LSSSLFDD912 pKa = 3.2 MSMRR916 pKa = 11.84 PAHH919 pKa = 5.3 YY920 pKa = 10.02 HH921 pKa = 4.98 SVAYY925 pKa = 10.09 LCKK928 pKa = 9.79 KK929 pKa = 10.4 LGMVTPQVTTAYY941 pKa = 8.56 TYY943 pKa = 10.25 SKK945 pKa = 10.25 EE946 pKa = 4.12 WFHH949 pKa = 6.31 SHH951 pKa = 7.32 PGMLKK956 pKa = 10.24 NIKK959 pKa = 9.7 KK960 pKa = 9.63 VLRR963 pKa = 11.84 KK964 pKa = 9.87 RR965 pKa = 11.84 MQFYY969 pKa = 10.16 HH970 pKa = 6.46 QDD972 pKa = 3.18 GGRR975 pKa = 11.84 KK976 pKa = 9.07 KK977 pKa = 10.22 FKK979 pKa = 10.91 KK980 pKa = 9.45 MLQNFKK986 pKa = 10.65 KK987 pKa = 10.12 IRR989 pKa = 11.84 QEE991 pKa = 4.21 FSFLYY996 pKa = 9.27 PNKK999 pKa = 9.7 PFTIHH1004 pKa = 6.33 NPDD1007 pKa = 3.94 IIAALL1012 pKa = 3.68
Molecular weight: 118.75 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 8.613
IPC2_protein 8.521
IPC_protein 8.361
Toseland 9.121
ProMoST 8.946
Dawson 9.428
Bjellqvist 9.209
Wikipedia 9.575
Rodwell 9.692
Grimsley 9.487
Solomon 9.443
Lehninger 9.414
Nozaki 9.341
DTASelect 9.136
Thurlkill 9.297
EMBOSS 9.575
Sillero 9.414
Patrickios 4.774
IPC_peptide 9.443
IPC2_peptide 7.775
IPC2.peptide.svr19 7.709
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
2
0
2
1302
290
1012
651.0
76.21
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
4.685 ± 0.732
1.075 ± 0.517
5.914 ± 0.357
7.066 ± 1.577
5.76 ± 0.449
4.147 ± 0.336
3.456 ± 0.17
5.453 ± 0.467
9.524 ± 0.269
9.37 ± 0.303
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.842 ± 0.126
3.763 ± 0.483
3.226 ± 0.556
3.763 ± 0.678
5.376 ± 0.098
7.757 ± 0.581
5.376 ± 0.234
5.684 ± 0.252
2.227 ± 0.09
3.533 ± 0.87
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here