Capybara microvirus Cap3_SP_465
Average proteome isoelectric point is 5.45
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 6 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A4P8W588|A0A4P8W588_9VIRU Major capsid protein OS=Capybara microvirus Cap3_SP_465 OX=2585462 PE=3 SV=1
MM1 pKa = 7.54 KK2 pKa = 10.18 IEE4 pKa = 4.5 FKK6 pKa = 11.13 NPLEE10 pKa = 4.96 IDD12 pKa = 3.27 TSSYY16 pKa = 11.39 VSVCGLPYY24 pKa = 10.61 EE25 pKa = 5.4 DD26 pKa = 6.06 DD27 pKa = 3.99 YY28 pKa = 12.05 QLSLDD33 pKa = 4.53 DD34 pKa = 5.3 NGCEE38 pKa = 3.96 YY39 pKa = 10.71 LKK41 pKa = 10.74 KK42 pKa = 10.62 VGVIEE47 pKa = 4.38 TYY49 pKa = 11.14 KK50 pKa = 10.65 LIQSHH55 pKa = 6.97 RR56 pKa = 11.84 DD57 pKa = 3.42 SCDD60 pKa = 2.78 LAYY63 pKa = 9.8 ILDD66 pKa = 4.04 TLDD69 pKa = 4.08 PDD71 pKa = 4.01 SVLGSGSPNVSFDD84 pKa = 4.64 DD85 pKa = 4.14 IYY87 pKa = 11.05 IDD89 pKa = 3.7 FTKK92 pKa = 10.63 FPTNPGEE99 pKa = 4.13 AFNLIKK105 pKa = 10.72 NAEE108 pKa = 3.99 FAFNKK113 pKa = 10.34 LPFEE117 pKa = 4.74 LRR119 pKa = 11.84 QEE121 pKa = 4.75 CNFSPNVFVKK131 pKa = 10.76 NLDD134 pKa = 3.35 KK135 pKa = 11.04 LAEE138 pKa = 4.32 KK139 pKa = 10.31 FSKK142 pKa = 9.87 VDD144 pKa = 3.12 KK145 pKa = 10.5 VEE147 pKa = 4.2 SVGVSNEE154 pKa = 3.56
Molecular weight: 17.4 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 4.347
IPC2_protein 4.355
IPC_protein 4.291
Toseland 4.101
ProMoST 4.38
Dawson 4.253
Bjellqvist 4.444
Wikipedia 4.151
Rodwell 4.113
Grimsley 4.012
Solomon 4.253
Lehninger 4.202
Nozaki 4.368
DTASelect 4.558
Thurlkill 4.126
EMBOSS 4.164
Sillero 4.406
Patrickios 3.541
IPC_peptide 4.253
IPC2_peptide 4.38
IPC2.peptide.svr19 4.346
Protein with the highest isoelectric point:
>tr|A0A4P8W543|A0A4P8W543_9VIRU Minor capsid protein OS=Capybara microvirus Cap3_SP_465 OX=2585462 PE=4 SV=1
MM1 pKa = 7.77 RR2 pKa = 11.84 SILEE6 pKa = 4.2 ASQSPNNWFITLTYY20 pKa = 10.94 DD21 pKa = 4.06 NDD23 pKa = 3.77 HH24 pKa = 6.64 LPISKK29 pKa = 10.06 YY30 pKa = 10.62 LNEE33 pKa = 4.04 EE34 pKa = 3.86 TGVYY38 pKa = 9.83 TEE40 pKa = 5.04 TPTLDD45 pKa = 3.39 NVEE48 pKa = 4.06 EE49 pKa = 4.2 MKK51 pKa = 10.96 KK52 pKa = 8.46 FWKK55 pKa = 10.06 RR56 pKa = 11.84 FRR58 pKa = 11.84 INLSRR63 pKa = 11.84 SSDD66 pKa = 3.04 VDD68 pKa = 3.75 LKK70 pKa = 11.28 VRR72 pKa = 11.84 YY73 pKa = 8.89 LYY75 pKa = 10.87 SGEE78 pKa = 4.34 YY79 pKa = 10.59 GDD81 pKa = 3.96 TTHH84 pKa = 7.29 RR85 pKa = 11.84 PHH87 pKa = 6.41 FHH89 pKa = 7.26 AILFNTVLPDD99 pKa = 3.62 LKK101 pKa = 10.99 VFYY104 pKa = 10.14 KK105 pKa = 10.68 DD106 pKa = 2.96 KK107 pKa = 10.23 QTGFTYY113 pKa = 9.3 YY114 pKa = 10.26 TSEE117 pKa = 4.37 LLEE120 pKa = 4.37 KK121 pKa = 10.0 SWKK124 pKa = 9.09 KK125 pKa = 11.02 GNVLIADD132 pKa = 4.27 CTFEE136 pKa = 3.95 TCQYY140 pKa = 8.4 VAAYY144 pKa = 9.24 VVKK147 pKa = 10.34 KK148 pKa = 7.93 QTGKK152 pKa = 10.27 QSQVYY157 pKa = 9.9 DD158 pKa = 4.17 DD159 pKa = 4.39 VNIIPPYY166 pKa = 7.81 MQMSRR171 pKa = 11.84 RR172 pKa = 11.84 PGIGSQVIEE181 pKa = 4.58 LNLEE185 pKa = 4.4 TFWHH189 pKa = 6.87 DD190 pKa = 3.17 KK191 pKa = 11.03 SIILSTKK198 pKa = 10.44 NGGKK202 pKa = 9.01 QLPFDD207 pKa = 3.68 QYY209 pKa = 11.45 IKK211 pKa = 11.25 NKK213 pKa = 9.85 LDD215 pKa = 3.31 KK216 pKa = 11.12 QFGEE220 pKa = 4.88 EE221 pKa = 3.86 YY222 pKa = 10.39 QEE224 pKa = 4.51 YY225 pKa = 10.62 KK226 pKa = 10.88 NQLCEE231 pKa = 4.67 DD232 pKa = 3.6 MKK234 pKa = 10.95 EE235 pKa = 4.12 LQEE238 pKa = 4.1 LKK240 pKa = 10.77 YY241 pKa = 11.0 GSLEE245 pKa = 3.91 KK246 pKa = 10.18 TYY248 pKa = 10.93 LQALLDD254 pKa = 3.74 EE255 pKa = 4.79 EE256 pKa = 5.71 ANFKK260 pKa = 11.14 ANYY263 pKa = 8.55 FKK265 pKa = 11.25 NKK267 pKa = 8.73 SRR269 pKa = 11.84 KK270 pKa = 8.87 LL271 pKa = 3.53
Molecular weight: 31.97 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 6.411
IPC2_protein 6.478
IPC_protein 6.722
Toseland 6.781
ProMoST 7.059
Dawson 7.19
Bjellqvist 6.971
Wikipedia 7.161
Rodwell 7.176
Grimsley 6.883
Solomon 7.249
Lehninger 7.263
Nozaki 7.278
DTASelect 7.424
Thurlkill 7.454
EMBOSS 7.527
Sillero 7.527
Patrickios 4.062
IPC_peptide 7.263
IPC2_peptide 6.854
IPC2.peptide.svr19 7.014
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
6
0
6
1463
82
561
243.8
27.21
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
7.177 ± 2.799
1.025 ± 0.296
6.083 ± 0.837
4.99 ± 1.007
4.785 ± 0.849
6.015 ± 0.77
1.846 ± 0.589
4.785 ± 0.387
5.947 ± 1.433
8.271 ± 0.895
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.392 ± 0.329
6.63 ± 1.038
3.623 ± 0.865
4.238 ± 0.741
3.008 ± 0.524
10.321 ± 1.578
6.015 ± 0.707
6.425 ± 0.675
1.025 ± 0.423
5.4 ± 0.554
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here