Mycobacterium phage JoieB

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; Marvinvirus; unclassified Marvinvirus

Average proteome isoelectric point is 6.0

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 117 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A5P8D4Z4|A0A5P8D4Z4_9CAUD Uncharacterized protein OS=Mycobacterium phage JoieB OX=2653277 GN=19 PE=4 SV=1
MM1 pKa = 7.44NAPEE5 pKa = 4.1LRR7 pKa = 11.84AVLTEE12 pKa = 4.1ALAGHH17 pKa = 5.59QPEE20 pKa = 4.6NYY22 pKa = 9.94GFNCSGCDD30 pKa = 3.4WEE32 pKa = 4.41PANPGVTYY40 pKa = 10.31AAEE43 pKa = 4.25FAAHH47 pKa = 6.25QLDD50 pKa = 3.58VLYY53 pKa = 10.17AVPGVAVIQLPEE65 pKa = 4.88PDD67 pKa = 5.07DD68 pKa = 3.77DD69 pKa = 5.7HH70 pKa = 9.06GDD72 pKa = 4.23GIAFWTAQEE81 pKa = 4.08VGVAGGMVVARR92 pKa = 11.84FDD94 pKa = 3.88PGNEE98 pKa = 3.83AIYY101 pKa = 10.33YY102 pKa = 7.43EE103 pKa = 4.59PSEE106 pKa = 4.06AWEE109 pKa = 4.11TAVALAAAAVVAEE122 pKa = 4.87GEE124 pKa = 4.39DD125 pKa = 3.55KK126 pKa = 11.29

Molecular weight: 13.25 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A5P8D549|A0A5P8D549_9CAUD Uncharacterized protein OS=Mycobacterium phage JoieB OX=2653277 GN=69 PE=4 SV=1
MM1 pKa = 7.44SNRR4 pKa = 11.84RR5 pKa = 11.84TVRR8 pKa = 11.84IRR10 pKa = 11.84IEE12 pKa = 4.03SNKK15 pKa = 10.26AKK17 pKa = 10.11TSFWYY22 pKa = 9.43TLVAGNGNTIMRR34 pKa = 11.84STAKK38 pKa = 9.45YY39 pKa = 4.77TTEE42 pKa = 3.97WNCRR46 pKa = 11.84TAATRR51 pKa = 11.84LCDD54 pKa = 3.71ALRR57 pKa = 11.84FSPLAVEE64 pKa = 4.4VQDD67 pKa = 3.66PKK69 pKa = 10.58TRR71 pKa = 11.84EE72 pKa = 3.43ISTLEE77 pKa = 4.25RR78 pKa = 11.84YY79 pKa = 8.41PSSPLKK85 pKa = 10.56VLQKK89 pKa = 7.78TTQTRR94 pKa = 11.84SS95 pKa = 2.92

Molecular weight: 10.9 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

117

0

117

20667

38

1840

176.6

19.57

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

9.755 ± 0.294

1.321 ± 0.184

6.556 ± 0.26

6.755 ± 0.308

3.319 ± 0.149

8.298 ± 0.492

2.163 ± 0.148

4.529 ± 0.164

3.803 ± 0.215

8.211 ± 0.246

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.627 ± 0.127

3.527 ± 0.205

5.802 ± 0.233

3.706 ± 0.248

6.769 ± 0.405

5.506 ± 0.268

5.613 ± 0.306

6.789 ± 0.207

2.255 ± 0.133

2.695 ± 0.165
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski