Escherichia phage MS2 (Bacteriophage MS2)

Taxonomy: Viruses; Riboviria; Orthornavirae; Lenarviricota; Allassoviricetes; Levivirales; Leviviridae; Levivirus

Average proteome isoelectric point is 8.42

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 4 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>sp|P03612|CAPSD_BPMS2 Capsid protein OS=Escherichia phage MS2 OX=329852 PE=1 SV=2
MM1 pKa = 7.8ASNFTQFVLVDD12 pKa = 3.32NGGTGDD18 pKa = 3.67VTVAPSNFANGVAEE32 pKa = 4.71WISSNSRR39 pKa = 11.84SQAYY43 pKa = 9.18KK44 pKa = 7.66VTCSVRR50 pKa = 11.84QSSAQNRR57 pKa = 11.84KK58 pKa = 6.94YY59 pKa = 8.88TIKK62 pKa = 10.9VEE64 pKa = 4.25VPKK67 pKa = 10.97VATQTVGGVEE77 pKa = 4.21LPVAAWRR84 pKa = 11.84SYY86 pKa = 11.56LNMEE90 pKa = 3.9LTIPIFATNSDD101 pKa = 3.79CEE103 pKa = 4.82LIVKK107 pKa = 10.01AMQGLLKK114 pKa = 10.45DD115 pKa = 4.02GNPIPSAIAANSGIYY130 pKa = 9.86

Molecular weight: 13.86 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>sp|P03610|MATA_BPMS2 Maturation protein A OS=Escherichia phage MS2 OX=329852 GN=A PE=1 SV=1
MM1 pKa = 7.21EE2 pKa = 4.57TRR4 pKa = 11.84FPQQSQQTPASTNRR18 pKa = 11.84RR19 pKa = 11.84RR20 pKa = 11.84PFKK23 pKa = 10.96HH24 pKa = 6.25EE25 pKa = 4.89DD26 pKa = 3.55YY27 pKa = 10.52PCRR30 pKa = 11.84RR31 pKa = 11.84QQRR34 pKa = 11.84SSTLYY39 pKa = 10.25VLIFLAIFLSKK50 pKa = 9.68FTNQLLLSLLEE61 pKa = 3.96AVIRR65 pKa = 11.84TVTTLQQLLTT75 pKa = 4.01

Molecular weight: 8.87 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

4

0

4

1143

75

545

285.8

31.89

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

8.136 ± 0.74

1.137 ± 0.152

4.462 ± 0.983

4.112 ± 0.087

4.637 ± 0.452

7.087 ± 1.021

1.4 ± 0.3

5.162 ± 0.527

4.112 ± 0.348

9.186 ± 0.988

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.925 ± 0.107

4.112 ± 0.675

4.724 ± 0.187

4.199 ± 1.229

7.262 ± 0.751

9.011 ± 0.217

6.649 ± 0.832

6.912 ± 0.862

2.012 ± 0.459

3.762 ± 0.205
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski