Klebsiella phage 1513
Average proteome isoelectric point is 6.5
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 72 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A0C5AN96|A0A0C5AN96_9CAUD Uncharacterized protein OS=Klebsiella phage 1513 OX=1610829 GN=PK_045 PE=4 SV=1
MM1 pKa = 7.67 KK2 pKa = 10.31 LYY4 pKa = 10.06 PDD6 pKa = 4.02 QNDD9 pKa = 3.27 PAAPMRR15 pKa = 11.84 LGDD18 pKa = 3.8 TPIDD22 pKa = 3.8 EE23 pKa = 4.66 QEE25 pKa = 4.3 VIAAIARR32 pKa = 11.84 ASDD35 pKa = 3.91 DD36 pKa = 3.93 EE37 pKa = 4.61 NPTALEE43 pKa = 4.32 CVTADD48 pKa = 3.91 LLLMSFLPEE57 pKa = 3.89 SEE59 pKa = 5.54 SYY61 pKa = 9.71 IQHH64 pKa = 5.29 ITEE67 pKa = 4.44 TGQAVTKK74 pKa = 10.2 PEE76 pKa = 4.41 FIGASNVLAMMADD89 pKa = 3.38 EE90 pKa = 4.69 SDD92 pKa = 4.05 NIISLDD98 pKa = 3.69 YY99 pKa = 10.39 PQQ101 pKa = 5.29
Molecular weight: 11.01 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 4.037
IPC2_protein 3.719
IPC_protein 3.656
Toseland 3.465
ProMoST 3.834
Dawson 3.656
Bjellqvist 3.808
Wikipedia 3.592
Rodwell 3.49
Grimsley 3.376
Solomon 3.63
Lehninger 3.579
Nozaki 3.783
DTASelect 3.961
Thurlkill 3.528
EMBOSS 3.605
Sillero 3.783
Patrickios 2.918
IPC_peptide 3.63
IPC2_peptide 3.757
IPC2.peptide.svr19 3.743
Protein with the highest isoelectric point:
>tr|A0A0C5ACP6|A0A0C5ACP6_9CAUD Uncharacterized protein OS=Klebsiella phage 1513 OX=1610829 GN=PK_071 PE=4 SV=1
MM1 pKa = 7.15 EE2 pKa = 4.35 QMAKK6 pKa = 9.16 IDD8 pKa = 3.69 KK9 pKa = 8.67 RR10 pKa = 11.84 TLNGNNGTSRR20 pKa = 11.84 GKK22 pKa = 10.13 DD23 pKa = 2.76 KK24 pKa = 10.96 KK25 pKa = 10.14 KK26 pKa = 10.08 RR27 pKa = 11.84 KK28 pKa = 9.64 RR29 pKa = 11.84 PTGYY33 pKa = 10.29 YY34 pKa = 8.98 VLKK37 pKa = 10.82 DD38 pKa = 3.64 EE39 pKa = 4.64 VRR41 pKa = 11.84 AGLTARR47 pKa = 11.84 MEE49 pKa = 4.17 LVIDD53 pKa = 4.09 AFGGIAGTAKK63 pKa = 10.28 EE64 pKa = 4.69 LGVSIQVVQQWRR76 pKa = 11.84 KK77 pKa = 10.07 RR78 pKa = 11.84 GMISADD84 pKa = 3.02 GAYY87 pKa = 9.93 LVHH90 pKa = 7.28 KK91 pKa = 9.81 SYY93 pKa = 10.98 RR94 pKa = 11.84 RR95 pKa = 11.84 NNCKK99 pKa = 9.96 GFRR102 pKa = 11.84 ASFCRR107 pKa = 11.84 PDD109 pKa = 4.47 LRR111 pKa = 11.84 FDD113 pKa = 4.29 SNGKK117 pKa = 8.82 PVTRR121 pKa = 11.84 RR122 pKa = 11.84 CDD124 pKa = 3.19 RR125 pKa = 11.84 RR126 pKa = 11.84 EE127 pKa = 3.64 MLRR130 pKa = 11.84 VVRR133 pKa = 5.17
Molecular weight: 15.21 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.335
IPC2_protein 9.765
IPC_protein 10.584
Toseland 10.921
ProMoST 10.804
Dawson 10.979
Bjellqvist 10.701
Wikipedia 11.199
Rodwell 11.228
Grimsley 11.023
Solomon 11.14
Lehninger 11.111
Nozaki 10.906
DTASelect 10.687
Thurlkill 10.906
EMBOSS 11.33
Sillero 10.921
Patrickios 10.95
IPC_peptide 11.155
IPC2_peptide 9.706
IPC2.peptide.svr19 8.677
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
72
0
72
13581
50
989
188.6
21.04
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
8.865 ± 0.414
1.451 ± 0.159
6.288 ± 0.23
6.421 ± 0.305
4.219 ± 0.152
7.812 ± 0.229
1.87 ± 0.196
6.222 ± 0.187
6.708 ± 0.317
6.693 ± 0.212
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
3.137 ± 0.146
4.492 ± 0.2
3.49 ± 0.229
3.696 ± 0.282
5.294 ± 0.254
6.045 ± 0.251
5.287 ± 0.236
7.054 ± 0.345
1.465 ± 0.118
3.49 ± 0.188
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here