Berrimah virus

Taxonomy: Viruses; Riboviria; Orthornavirae; Negarnaviricota; Haploviricotina; Monjiviricetes; Mononegavirales; Rhabdoviridae; Alpharhabdovirinae; Ephemerovirus; Berrimah ephemerovirus

Average proteome isoelectric point is 7.02

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 10 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|I3NUX6|I3NUX6_9RHAB Matrix protein M OS=Berrimah virus OX=318834 GN=M PE=4 SV=1
MM1 pKa = 7.25SHH3 pKa = 6.33QKK5 pKa = 9.93VKK7 pKa = 10.89LMTGTYY13 pKa = 10.29DD14 pKa = 3.34AGKK17 pKa = 9.36LQRR20 pKa = 11.84NLQEE24 pKa = 5.08HH25 pKa = 6.58ILLEE29 pKa = 4.24EE30 pKa = 4.9DD31 pKa = 3.47EE32 pKa = 5.58DD33 pKa = 4.88GSFAQGDD40 pKa = 3.82QVAEE44 pKa = 4.6EE45 pKa = 4.32ILPINPLLNKK55 pKa = 9.91SEE57 pKa = 4.06PKK59 pKa = 10.34VYY61 pKa = 10.0PIEE64 pKa = 4.68INLEE68 pKa = 4.08DD69 pKa = 4.0LEE71 pKa = 4.38KK72 pKa = 11.33ANGIEE77 pKa = 4.57DD78 pKa = 3.83DD79 pKa = 4.21WEE81 pKa = 4.77HH82 pKa = 6.5SLLKK86 pKa = 10.12IIEE89 pKa = 4.24SSEE92 pKa = 3.98VSPKK96 pKa = 10.72LSWDD100 pKa = 3.85DD101 pKa = 3.68EE102 pKa = 4.73FEE104 pKa = 4.33SDD106 pKa = 5.42SYY108 pKa = 11.87EE109 pKa = 3.99KK110 pKa = 10.5DD111 pKa = 3.13KK112 pKa = 11.62KK113 pKa = 10.9GMRR116 pKa = 11.84GPKK119 pKa = 9.18QMKK122 pKa = 9.92DD123 pKa = 2.96IQNKK127 pKa = 9.16YY128 pKa = 9.31IVEE131 pKa = 4.01IDD133 pKa = 3.49EE134 pKa = 5.78DD135 pKa = 4.02SLKK138 pKa = 11.14DD139 pKa = 3.6VLKK142 pKa = 10.78VLSAFEE148 pKa = 3.9IRR150 pKa = 11.84EE151 pKa = 4.19GEE153 pKa = 4.17DD154 pKa = 3.38FNVSNNKK161 pKa = 9.29SKK163 pKa = 10.57RR164 pKa = 11.84VKK166 pKa = 10.15IVIEE170 pKa = 3.79KK171 pKa = 10.53RR172 pKa = 11.84GTRR175 pKa = 11.84EE176 pKa = 3.73CNKK179 pKa = 9.68IDD181 pKa = 3.64VTGKK185 pKa = 9.98SPQNEE190 pKa = 3.74AMKK193 pKa = 10.37EE194 pKa = 3.88DD195 pKa = 3.65RR196 pKa = 11.84SKK198 pKa = 11.15FDD200 pKa = 3.44QIMDD204 pKa = 3.31QFQRR208 pKa = 11.84GIRR211 pKa = 11.84IKK213 pKa = 10.66KK214 pKa = 9.86KK215 pKa = 9.63FGKK218 pKa = 10.57GYY220 pKa = 11.25VKK222 pKa = 10.33MNADD226 pKa = 3.77NMPGTYY232 pKa = 9.65HH233 pKa = 6.86EE234 pKa = 5.24LYY236 pKa = 10.28SVVSGIVDD244 pKa = 3.59EE245 pKa = 4.56NTTEE249 pKa = 5.3DD250 pKa = 3.4MIKK253 pKa = 10.61YY254 pKa = 9.66LFRR257 pKa = 11.84KK258 pKa = 9.5SKK260 pKa = 10.47SFKK263 pKa = 11.25AMGKK267 pKa = 8.32MLNIDD272 pKa = 4.51EE273 pKa = 5.76IILCC277 pKa = 4.54

Molecular weight: 31.96 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|I3NUX9|I3NUX9_9RHAB Isoform of I3NUY0 Alpha 1 protein OS=Berrimah virus OX=318834 GN=alpha PE=4 SV=1
MM1 pKa = 7.91DD2 pKa = 5.77RR3 pKa = 11.84SLLSNFWKK11 pKa = 10.69DD12 pKa = 3.22FTNWSEE18 pKa = 3.61ARR20 pKa = 11.84KK21 pKa = 9.86IEE23 pKa = 5.23IIDD26 pKa = 2.88WWTNLEE32 pKa = 3.74GRR34 pKa = 11.84IRR36 pKa = 11.84LGFWIIFIILIGLIIIRR53 pKa = 11.84ISYY56 pKa = 9.9KK57 pKa = 9.84ILKK60 pKa = 9.53CIQLLRR66 pKa = 11.84KK67 pKa = 10.14GIVKK71 pKa = 8.65TKK73 pKa = 10.53RR74 pKa = 11.84IIKK77 pKa = 9.39KK78 pKa = 9.47RR79 pKa = 11.84KK80 pKa = 7.02TIKK83 pKa = 10.25KK84 pKa = 8.21YY85 pKa = 10.24RR86 pKa = 11.84KK87 pKa = 8.8HH88 pKa = 5.82

Molecular weight: 10.83 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

9

1

10

4745

88

2144

474.5

55.07

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

2.929 ± 0.426

1.855 ± 0.268

5.606 ± 0.48

8.114 ± 0.513

3.983 ± 0.3

5.564 ± 0.274

2.234 ± 0.165

8.746 ± 0.772

8.809 ± 0.682

9.505 ± 0.803

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.655 ± 0.271

6.596 ± 0.418

3.182 ± 0.213

2.655 ± 0.241

5.016 ± 0.353

7.503 ± 0.494

4.215 ± 0.303

4.953 ± 0.431

1.812 ± 0.267

4.067 ± 0.378
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski