Amino acid dipepetide frequency for Sarcina sp. DSM 11001

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10^-3.

For more information see sequence space article on Wikipedia.

	Ala	Cys	Asp	Glu	Phe	Gly	His	Ile	Lys	Leu	Met	Asn	Pro	Gln	Arg	Ser	Thr	Val	Trp	Tyr	Xaa
Ala	10.024AlaAla: 10.024 ± 0.182	1.274AlaCys: 1.274 ± 0.04	5.608AlaAsp: 5.608 ± 0.092	6.39AlaGlu: 6.39 ± 0.103	3.343AlaPhe: 3.343 ± 0.066	7.972AlaGly: 7.972 ± 0.115	1.202AlaHis: 1.202 ± 0.03	4.128AlaIle: 4.128 ± 0.066	3.836AlaLys: 3.836 ± 0.075	7.072AlaLeu: 7.072 ± 0.106	2.176AlaMet: 2.176 ± 0.051	2.239AlaAsn: 2.239 ± 0.048	2.662AlaPro: 2.662 ± 0.06	2.658AlaGln: 2.658 ± 0.05	3.986AlaArg: 3.986 ± 0.068	4.686AlaSer: 4.686 ± 0.079	2.99AlaThr: 2.99 ± 0.067	7.009AlaVal: 7.009 ± 0.103	0.759AlaTrp: 0.759 ± 0.027	2.642AlaTyr: 2.642 ± 0.05	0.001AlaXaa: 0.001 ± 0.001
Cys	1.208CysAla: 1.208 ± 0.037	0.257CysCys: 0.257 ± 0.017	0.786CysAsp: 0.786 ± 0.028	0.838CysGlu: 0.838 ± 0.027	0.645CysPhe: 0.645 ± 0.021	1.567CysGly: 1.567 ± 0.039	0.271CysHis: 0.271 ± 0.017	0.936CysIle: 0.936 ± 0.033	0.586CysLys: 0.586 ± 0.025	1.237CysLeu: 1.237 ± 0.036	0.425CysMet: 0.425 ± 0.021	0.455CysAsn: 0.455 ± 0.022	0.717CysPro: 0.717 ± 0.03	0.33CysGln: 0.33 ± 0.018	1.04CysArg: 1.04 ± 0.034	0.99CysSer: 0.99 ± 0.032	0.823CysThr: 0.823 ± 0.029	0.951CysVal: 0.951 ± 0.028	0.136CysTrp: 0.136 ± 0.012	0.538CysTyr: 0.538 ± 0.024	0.0CysXaa: 0.0 ± 0.0
Asp	4.819AspAla: 4.819 ± 0.074	0.816AspCys: 0.816 ± 0.027	3.406AspAsp: 3.406 ± 0.074	4.754AspGlu: 4.754 ± 0.085	2.617AspPhe: 2.617 ± 0.048	5.173AspGly: 5.173 ± 0.111	1.123AspHis: 1.123 ± 0.038	4.007AspIle: 4.007 ± 0.067	2.998AspLys: 2.998 ± 0.053	5.348AspLeu: 5.348 ± 0.07	1.624AspMet: 1.624 ± 0.039	2.239AspAsn: 2.239 ± 0.054	3.095AspPro: 3.095 ± 0.059	1.849AspGln: 1.849 ± 0.047	3.599AspArg: 3.599 ± 0.073	3.968AspSer: 3.968 ± 0.084	3.333AspThr: 3.333 ± 0.065	3.652AspVal: 3.652 ± 0.059	0.675AspTrp: 0.675 ± 0.024	2.715AspTyr: 2.715 ± 0.056	0.0AspXaa: 0.0 ± 0.0
Glu	6.547GluAla: 6.547 ± 0.096	0.735GluCys: 0.735 ± 0.028	5.139GluAsp: 5.139 ± 0.09	7.915GluGlu: 7.915 ± 0.13	2.136GluPhe: 2.136 ± 0.052	5.036GluGly: 5.036 ± 0.084	1.222GluHis: 1.222 ± 0.034	5.288GluIle: 5.288 ± 0.08	6.293GluLys: 6.293 ± 0.088	5.823GluLeu: 5.823 ± 0.092	2.255GluMet: 2.255 ± 0.048	3.989GluAsn: 3.989 ± 0.084	2.268GluPro: 2.268 ± 0.047	2.626GluGln: 2.626 ± 0.056	3.423GluArg: 3.423 ± 0.069	3.75GluSer: 3.75 ± 0.067	4.517GluThr: 4.517 ± 0.099	4.003GluVal: 4.003 ± 0.061	0.662GluTrp: 0.662 ± 0.027	2.89GluTyr: 2.89 ± 0.056	0.0GluXaa: 0.0 ± 0.0
Phe	2.929PheAla: 2.929 ± 0.057	0.733PheCys: 0.733 ± 0.03	2.539PheAsp: 2.539 ± 0.044	2.526PheGlu: 2.526 ± 0.055	1.83PhePhe: 1.83 ± 0.055	2.833PheGly: 2.833 ± 0.065	0.819PheHis: 0.819 ± 0.029	2.377PheIle: 2.377 ± 0.056	1.661PheLys: 1.661 ± 0.037	3.814PheLeu: 3.814 ± 0.069	1.03PheMet: 1.03 ± 0.034	1.408PheAsn: 1.408 ± 0.037	1.583PhePro: 1.583 ± 0.036	1.014PheGln: 1.014 ± 0.029	2.317PheArg: 2.317 ± 0.054	3.132PheSer: 3.132 ± 0.063	2.414PheThr: 2.414 ± 0.046	2.506PheVal: 2.506 ± 0.047	0.394PheTrp: 0.394 ± 0.022	1.561PheTyr: 1.561 ± 0.038	0.0PheXaa: 0.0 ± 0.0
Gly	5.626GlyAla: 5.626 ± 0.097	1.232GlyCys: 1.232 ± 0.037	4.14GlyAsp: 4.14 ± 0.065	5.223GlyGlu: 5.223 ± 0.076	3.245GlyPhe: 3.245 ± 0.054	5.543GlyGly: 5.543 ± 0.122	1.384GlyHis: 1.384 ± 0.033	5.935GlyIle: 5.935 ± 0.082	5.215GlyLys: 5.215 ± 0.083	5.883GlyLeu: 5.883 ± 0.072	2.303GlyMet: 2.303 ± 0.05	3.307GlyAsn: 3.307 ± 0.075	1.779GlyPro: 1.779 ± 0.051	2.364GlyGln: 2.364 ± 0.057	4.326GlyArg: 4.326 ± 0.075	5.332GlySer: 5.332 ± 0.086	5.296GlyThr: 5.296 ± 0.118	4.477GlyVal: 4.477 ± 0.079	0.748GlyTrp: 0.748 ± 0.033	3.027GlyTyr: 3.027 ± 0.06	0.0GlyXaa: 0.0 ± 0.0
His	1.182HisAla: 1.182 ± 0.032	0.306HisCys: 0.306 ± 0.018	0.996HisAsp: 0.996 ± 0.037	1.041HisGlu: 1.041 ± 0.032	0.806HisPhe: 0.806 ± 0.028	1.316HisGly: 1.316 ± 0.033	0.434HisHis: 0.434 ± 0.033	1.196HisIle: 1.196 ± 0.035	0.757HisLys: 0.757 ± 0.026	1.573HisLeu: 1.573 ± 0.043	0.536HisMet: 0.536 ± 0.019	0.633HisAsn: 0.633 ± 0.026	0.965HisPro: 0.965 ± 0.03	0.511HisGln: 0.511 ± 0.022	1.013HisArg: 1.013 ± 0.028	1.045HisSer: 1.045 ± 0.035	1.026HisThr: 1.026 ± 0.036	1.094HisVal: 1.094 ± 0.031	0.176HisTrp: 0.176 ± 0.013	0.827HisTyr: 0.827 ± 0.028	0.0HisXaa: 0.0 ± 0.0
Ile	4.977IleAla: 4.977 ± 0.071	1.194IleCys: 1.194 ± 0.04	3.959IleAsp: 3.959 ± 0.067	4.107IleGlu: 4.107 ± 0.072	2.389IlePhe: 2.389 ± 0.05	4.573IleGly: 4.573 ± 0.096	1.307IleHis: 1.307 ± 0.029	3.855IleIle: 3.855 ± 0.077	2.825IleLys: 2.825 ± 0.058	6.513IleLeu: 6.513 ± 0.107	1.581IleMet: 1.581 ± 0.04	2.407IleAsn: 2.407 ± 0.054	3.312IlePro: 3.312 ± 0.058	1.746IleGln: 1.746 ± 0.041	4.599IleArg: 4.599 ± 0.081	4.516IleSer: 4.516 ± 0.061	3.898IleThr: 3.898 ± 0.07	4.201IleVal: 4.201 ± 0.074	0.541IleTrp: 0.541 ± 0.023	2.316IleTyr: 2.316 ± 0.041	0.0IleXaa: 0.0 ± 0.0
Lys	4.836LysAla: 4.836 ± 0.072	0.577LysCys: 0.577 ± 0.027	3.587LysAsp: 3.587 ± 0.078	5.192LysGlu: 5.192 ± 0.087	1.305LysPhe: 1.305 ± 0.039	3.923LysGly: 3.923 ± 0.066	0.811LysHis: 0.811 ± 0.025	3.588LysIle: 3.588 ± 0.064	5.216LysLys: 5.216 ± 0.097	4.0LysLeu: 4.0 ± 0.07	1.643LysMet: 1.643 ± 0.041	2.889LysAsn: 2.889 ± 0.05	1.889LysPro: 1.889 ± 0.049	1.705LysGln: 1.705 ± 0.048	2.65LysArg: 2.65 ± 0.057	2.891LysSer: 2.891 ± 0.057	3.66LysThr: 3.66 ± 0.074	3.374LysVal: 3.374 ± 0.073	0.555LysTrp: 0.555 ± 0.023	2.255LysTyr: 2.255 ± 0.052	0.0LysXaa: 0.0 ± 0.0
Leu	6.643LeuAla: 6.643 ± 0.09	1.535LeuCys: 1.535 ± 0.042	4.826LeuAsp: 4.826 ± 0.064	5.926LeuGlu: 5.926 ± 0.082	3.663LeuPhe: 3.663 ± 0.071	5.328LeuGly: 5.328 ± 0.08	1.64LeuHis: 1.64 ± 0.038	5.378LeuIle: 5.378 ± 0.093	4.663LeuLys: 4.663 ± 0.086	8.153LeuLeu: 8.153 ± 0.128	2.35LeuMet: 2.35 ± 0.05	3.131LeuAsn: 3.131 ± 0.054	4.026LeuPro: 4.026 ± 0.063	2.894LeuGln: 2.894 ± 0.054	5.079LeuArg: 5.079 ± 0.084	6.152LeuSer: 6.152 ± 0.081	5.291LeuThr: 5.291 ± 0.074	4.996LeuVal: 4.996 ± 0.081	0.675LeuTrp: 0.675 ± 0.023	3.202LeuTyr: 3.202 ± 0.061	0.0LeuXaa: 0.0 ± 0.0
Met	2.192MetAla: 2.192 ± 0.051	0.315MetCys: 0.315 ± 0.017	1.807MetAsp: 1.807 ± 0.036	2.267MetGlu: 2.267 ± 0.05	0.89MetPhe: 0.89 ± 0.027	1.835MetGly: 1.835 ± 0.044	0.505MetHis: 0.505 ± 0.02	1.982MetIle: 1.982 ± 0.047	1.959MetLys: 1.959 ± 0.046	2.315MetLeu: 2.315 ± 0.047	0.859MetMet: 0.859 ± 0.029	1.273MetAsn: 1.273 ± 0.035	1.165MetPro: 1.165 ± 0.035	0.969MetGln: 0.969 ± 0.03	1.424MetArg: 1.424 ± 0.039	1.545MetSer: 1.545 ± 0.04	1.837MetThr: 1.837 ± 0.042	1.645MetVal: 1.645 ± 0.041	0.192MetTrp: 0.192 ± 0.015	0.811MetTyr: 0.811 ± 0.029	0.0MetXaa: 0.0 ± 0.0
Asn	3.109AsnAla: 3.109 ± 0.059	0.559AsnCys: 0.559 ± 0.022	2.071AsnAsp: 2.071 ± 0.051	2.49AsnGlu: 2.49 ± 0.054	1.209AsnPhe: 1.209 ± 0.037	3.509AsnGly: 3.509 ± 0.08	0.688AsnHis: 0.688 ± 0.021	2.702AsnIle: 2.702 ± 0.05	2.04AsnLys: 2.04 ± 0.041	3.133AsnLeu: 3.133 ± 0.056	1.05AsnMet: 1.05 ± 0.036	1.669AsnAsn: 1.669 ± 0.049	1.94AsnPro: 1.94 ± 0.047	1.07AsnGln: 1.07 ± 0.034	2.226AsnArg: 2.226 ± 0.05	2.313AsnSer: 2.313 ± 0.063	2.441AsnThr: 2.441 ± 0.056	2.449AsnVal: 2.449 ± 0.054	0.395AsnTrp: 0.395 ± 0.019	1.57AsnTyr: 1.57 ± 0.04	0.0AsnXaa: 0.0 ± 0.0
Pro	3.974ProAla: 3.974 ± 0.087	0.499ProCys: 0.499 ± 0.023	3.295ProAsp: 3.295 ± 0.063	4.302ProGlu: 4.302 ± 0.066	1.677ProPhe: 1.677 ± 0.039	3.349ProGly: 3.349 ± 0.059	0.592ProHis: 0.592 ± 0.024	1.725ProIle: 1.725 ± 0.041	1.484ProLys: 1.484 ± 0.039	2.956ProLeu: 2.956 ± 0.057	0.891ProMet: 0.891 ± 0.028	1.05ProAsn: 1.05 ± 0.033	0.984ProPro: 0.984 ± 0.036	1.163ProGln: 1.163 ± 0.039	1.47ProArg: 1.47 ± 0.04	2.284ProSer: 2.284 ± 0.048	1.45ProThr: 1.45 ± 0.033	3.635ProVal: 3.635 ± 0.062	0.412ProTrp: 0.412 ± 0.022	1.432ProTyr: 1.432 ± 0.037	0.0ProXaa: 0.0 ± 0.0
Gln	2.5GlnAla: 2.5 ± 0.048	0.329GlnCys: 0.329 ± 0.016	1.887GlnAsp: 1.887 ± 0.045	2.76GlnGlu: 2.76 ± 0.061	1.032GlnPhe: 1.032 ± 0.037	1.881GlnGly: 1.881 ± 0.049	0.45GlnHis: 0.45 ± 0.023	2.308GlnIle: 2.308 ± 0.048	2.346GlnLys: 2.346 ± 0.054	2.304GlnLeu: 2.304 ± 0.051	1.081GlnMet: 1.081 ± 0.028	1.407GlnAsn: 1.407 ± 0.039	1.025GlnPro: 1.025 ± 0.033	1.115GlnGln: 1.115 ± 0.045	1.417GlnArg: 1.417 ± 0.038	1.683GlnSer: 1.683 ± 0.045	1.868GlnThr: 1.868 ± 0.044	1.79GlnVal: 1.79 ± 0.043	0.297GlnTrp: 0.297 ± 0.015	1.309GlnTyr: 1.309 ± 0.04	0.0GlnXaa: 0.0 ± 0.0
Arg	3.721ArgAla: 3.721 ± 0.068	0.697ArgCys: 0.697 ± 0.028	3.201ArgAsp: 3.201 ± 0.068	4.748ArgGlu: 4.748 ± 0.083	2.406ArgPhe: 2.406 ± 0.051	3.284ArgGly: 3.284 ± 0.068	1.004ArgHis: 1.004 ± 0.03	4.448ArgIle: 4.448 ± 0.083	3.6ArgLys: 3.6 ± 0.071	4.715ArgLeu: 4.715 ± 0.073	1.776ArgMet: 1.776 ± 0.041	2.13ArgAsn: 2.13 ± 0.044	1.903ArgPro: 1.903 ± 0.043	2.053ArgGln: 2.053 ± 0.053	3.635ArgArg: 3.635 ± 0.073	3.044ArgSer: 3.044 ± 0.058	3.054ArgThr: 3.054 ± 0.059	3.162ArgVal: 3.162 ± 0.049	0.492ArgTrp: 0.492 ± 0.024	2.136ArgTyr: 2.136 ± 0.046	0.0ArgXaa: 0.0 ± 0.0
Ser	5.314SerAla: 5.314 ± 0.092	0.914SerCys: 0.914 ± 0.031	3.836SerAsp: 3.836 ± 0.066	4.369SerGlu: 4.369 ± 0.087	2.891SerPhe: 2.891 ± 0.057	6.347SerGly: 6.347 ± 0.095	0.974SerHis: 0.974 ± 0.028	3.671SerIle: 3.671 ± 0.059	2.605SerLys: 2.605 ± 0.042	5.454SerLeu: 5.454 ± 0.085	1.673SerMet: 1.673 ± 0.039	2.007SerAsn: 2.007 ± 0.053	2.368SerPro: 2.368 ± 0.049	1.736SerGln: 1.736 ± 0.038	3.66SerArg: 3.66 ± 0.073	4.306SerSer: 4.306 ± 0.079	3.185SerThr: 3.185 ± 0.059	4.381SerVal: 4.381 ± 0.06	0.707SerTrp: 0.707 ± 0.027	2.257SerTyr: 2.257 ± 0.051	0.0SerXaa: 0.0 ± 0.0
Thr	5.706ThrAla: 5.706 ± 0.113	0.785ThrCys: 0.785 ± 0.029	3.911ThrAsp: 3.911 ± 0.087	4.147ThrGlu: 4.147 ± 0.096	2.282ThrPhe: 2.282 ± 0.042	5.801ThrGly: 5.801 ± 0.089	0.852ThrHis: 0.852 ± 0.03	3.621ThrIle: 3.621 ± 0.072	2.495ThrLys: 2.495 ± 0.068	4.629ThrLeu: 4.629 ± 0.069	1.419ThrMet: 1.419 ± 0.039	1.812ThrAsn: 1.812 ± 0.053	2.386ThrPro: 2.386 ± 0.047	1.511ThrGln: 1.511 ± 0.039	2.452ThrArg: 2.452 ± 0.046	3.256ThrSer: 3.256 ± 0.065	2.864ThrThr: 2.864 ± 0.117	4.833ThrVal: 4.833 ± 0.102	0.587ThrTrp: 0.587 ± 0.023	2.076ThrTyr: 2.076 ± 0.059	0.0ThrXaa: 0.0 ± 0.0
Val	3.967ValAla: 3.967 ± 0.082	1.191ValCys: 1.191 ± 0.038	3.625ValAsp: 3.625 ± 0.069	4.127ValGlu: 4.127 ± 0.075	3.078ValPhe: 3.078 ± 0.055	3.725ValGly: 3.725 ± 0.069	1.182ValHis: 1.182 ± 0.03	4.666ValIle: 4.666 ± 0.074	3.522ValLys: 3.522 ± 0.056	6.312ValLeu: 6.312 ± 0.093	1.821ValMet: 1.821 ± 0.047	2.526ValAsn: 2.526 ± 0.059	2.92ValPro: 2.92 ± 0.055	1.966ValGln: 1.966 ± 0.04	3.998ValArg: 3.998 ± 0.069	4.735ValSer: 4.735 ± 0.068	4.479ValThr: 4.479 ± 0.11	3.981ValVal: 3.981 ± 0.076	0.616ValTrp: 0.616 ± 0.025	2.557ValTyr: 2.557 ± 0.049	0.0ValXaa: 0.0 ± 0.0
Trp	0.557TrpAla: 0.557 ± 0.024	0.165TrpCys: 0.165 ± 0.013	0.568TrpAsp: 0.568 ± 0.027	0.728TrpGlu: 0.728 ± 0.026	0.39TrpPhe: 0.39 ± 0.02	0.559TrpGly: 0.559 ± 0.024	0.208TrpHis: 0.208 ± 0.013	0.693TrpIle: 0.693 ± 0.029	0.708TrpLys: 0.708 ± 0.029	0.904TrpLeu: 0.904 ± 0.03	0.295TrpMet: 0.295 ± 0.018	0.539TrpAsn: 0.539 ± 0.025	0.251TrpPro: 0.251 ± 0.015	0.354TrpGln: 0.354 ± 0.02	0.472TrpArg: 0.472 ± 0.018	0.568TrpSer: 0.568 ± 0.026	0.539TrpThr: 0.539 ± 0.025	0.486TrpVal: 0.486 ± 0.021	0.114TrpTrp: 0.114 ± 0.012	0.438TrpTyr: 0.438 ± 0.022	0.0TrpXaa: 0.0 ± 0.0
Tyr	2.825TyrAla: 2.825 ± 0.062	0.622TyrCys: 0.622 ± 0.023	2.621TyrAsp: 2.621 ± 0.054	2.668TyrGlu: 2.668 ± 0.047	1.587TyrPhe: 1.587 ± 0.038	2.829TyrGly: 2.829 ± 0.049	0.765TyrHis: 0.765 ± 0.026	2.311TyrIle: 2.311 ± 0.047	1.737TyrLys: 1.737 ± 0.044	3.415TyrLeu: 3.415 ± 0.068	0.998TyrMet: 0.998 ± 0.031	1.525TyrAsn: 1.525 ± 0.04	1.387TyrPro: 1.387 ± 0.036	1.152TyrGln: 1.152 ± 0.035	2.424TyrArg: 2.424 ± 0.053	2.459TyrSer: 2.459 ± 0.054	2.461TyrThr: 2.461 ± 0.067	2.366TyrVal: 2.366 ± 0.05	0.403TyrTrp: 0.403 ± 0.018	1.919TyrTyr: 1.919 ± 0.062	0.0TyrXaa: 0.0 ± 0.0
Xaa	0.0XaaAla: 0.0 ± 0.0	0.0XaaCys: 0.0 ± 0.0	0.0XaaAsp: 0.0 ± 0.0	0.001XaaGlu: 0.001 ± 0.001	0.0XaaPhe: 0.0 ± 0.0	0.0XaaGly: 0.0 ± 0.0	0.0XaaHis: 0.0 ± 0.0	0.0XaaIle: 0.0 ± 0.0	0.0XaaLys: 0.0 ± 0.0	0.0XaaLeu: 0.0 ± 0.0	0.0XaaMet: 0.0 ± 0.0	0.0XaaAsn: 0.0 ± 0.0	0.0XaaPro: 0.0 ± 0.0	0.0XaaGln: 0.0 ± 0.0	0.0XaaArg: 0.0 ± 0.0	0.0XaaSer: 0.0 ± 0.0	0.0XaaThr: 0.0 ± 0.0	0.0XaaVal: 0.0 ± 0.0	0.0XaaTrp: 0.0 ± 0.0	0.0XaaTyr: 0.0 ± 0.0	0.003XaaXaa: 0.003 ± 0.002

Statistics based on 3187 proteins (1153430 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944

Contact: Lukasz P. Kozlowski

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