Salmonella phage SJ46
Average proteome isoelectric point is 6.45
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 122 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A1B0V861|A0A1B0V861_9CAUD Uncharacterized protein OS=Salmonella phage SJ46 OX=1815968 GN=J46_0083 PE=4 SV=1
MM1 pKa = 7.54 AFIPPTIDD9 pKa = 4.44 DD10 pKa = 3.84 VRR12 pKa = 11.84 HH13 pKa = 6.05 CSNALSVDD21 pKa = 3.55 PAEE24 pKa = 4.25 TDD26 pKa = 3.0 AARR29 pKa = 11.84 AIAEE33 pKa = 4.36 HH34 pKa = 6.3 YY35 pKa = 10.88 SKK37 pKa = 10.62 ISNQEE42 pKa = 3.46 YY43 pKa = 10.42 RR44 pKa = 11.84 ITQDD48 pKa = 4.89 DD49 pKa = 4.74 LDD51 pKa = 5.05 DD52 pKa = 4.06 LTDD55 pKa = 4.01 TIEE58 pKa = 4.42 YY59 pKa = 10.89 LMATNQPDD67 pKa = 3.65 SQQ69 pKa = 3.69
Molecular weight: 7.74 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 4.018
IPC2_protein 3.948
IPC_protein 3.897
Toseland 3.681
ProMoST 4.075
Dawson 3.897
Bjellqvist 4.062
Wikipedia 3.872
Rodwell 3.719
Grimsley 3.592
Solomon 3.884
Lehninger 3.846
Nozaki 4.024
DTASelect 4.279
Thurlkill 3.757
EMBOSS 3.872
Sillero 4.024
Patrickios 3.058
IPC_peptide 3.884
IPC2_peptide 3.999
IPC2.peptide.svr19 3.934
Protein with the highest isoelectric point:
>tr|A0A1B0V7H9|A0A1B0V7H9_9CAUD Transposase OS=Salmonella phage SJ46 OX=1815968 GN=tnpA PE=4 SV=1
MM1 pKa = 7.42 NKK3 pKa = 9.59 LRR5 pKa = 11.84 LLRR8 pKa = 11.84 RR9 pKa = 11.84 LSTMKK14 pKa = 10.64 LSLAAIVFDD23 pKa = 4.49 SIFMAVYY30 pKa = 9.91 VLNEE34 pKa = 4.0 TWPLEE39 pKa = 3.95 PLLYY43 pKa = 10.67 AGLRR47 pKa = 11.84 LCLTFLSMAARR58 pKa = 11.84 LMQQKK63 pKa = 7.9 EE64 pKa = 4.23 TASDD68 pKa = 3.91 CPGRR72 pKa = 11.84 AVRR75 pKa = 11.84 KK76 pKa = 8.79 YY77 pKa = 6.97 MARR80 pKa = 11.84 RR81 pKa = 11.84 RR82 pKa = 11.84 RR83 pKa = 3.46
Molecular weight: 9.67 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.301
IPC2_protein 9.911
IPC_protein 10.979
Toseland 10.994
ProMoST 11.374
Dawson 11.067
Bjellqvist 10.906
Wikipedia 11.389
Rodwell 11.096
Grimsley 11.111
Solomon 11.33
Lehninger 11.272
Nozaki 10.994
DTASelect 10.891
Thurlkill 11.008
EMBOSS 11.433
Sillero 11.023
Patrickios 10.862
IPC_peptide 11.33
IPC2_peptide 10.218
IPC2.peptide.svr19 8.79
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
122
0
122
30880
35
1493
253.1
28.16
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
9.621 ± 0.268
0.988 ± 0.105
6.062 ± 0.18
6.671 ± 0.199
3.507 ± 0.118
6.813 ± 0.229
1.745 ± 0.114
5.787 ± 0.14
5.755 ± 0.209
8.808 ± 0.234
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.542 ± 0.113
4.582 ± 0.177
3.996 ± 0.142
4.135 ± 0.126
5.991 ± 0.215
6.49 ± 0.162
5.738 ± 0.203
6.273 ± 0.139
1.367 ± 0.095
3.128 ± 0.144
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here