Rodent stool-associated circular genome virus
Average proteome isoelectric point is 6.06
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 1 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|G1C9H6|G1C9H6_9VIRU ATP-dependent helicase Rep OS=Rodent stool-associated circular genome virus OX=1074214 PE=3 SV=1
MM1 pKa = 7.64 CVRR4 pKa = 11.84 IKK6 pKa = 8.29 MTQATWWLGTKK17 pKa = 9.83 FVHH20 pKa = 6.32 EE21 pKa = 4.14 AHH23 pKa = 6.84 DD24 pKa = 3.79 TEE26 pKa = 4.15 EE27 pKa = 3.96 WMRR30 pKa = 11.84 DD31 pKa = 3.11 LAEE34 pKa = 4.24 SGKK37 pKa = 10.62 VKK39 pKa = 10.7 FITGQRR45 pKa = 11.84 EE46 pKa = 4.14 LCPEE50 pKa = 4.37 TMRR53 pKa = 11.84 HH54 pKa = 5.02 HH55 pKa = 5.8 VQFVVNLHH63 pKa = 6.06 RR64 pKa = 11.84 SQRR67 pKa = 11.84 LSYY70 pKa = 10.2 LKK72 pKa = 10.62 KK73 pKa = 9.93 IDD75 pKa = 5.13 GEE77 pKa = 4.38 AHH79 pKa = 5.71 WEE81 pKa = 4.37 SVRR84 pKa = 11.84 GTPKK88 pKa = 10.09 QARR91 pKa = 11.84 DD92 pKa = 3.51 YY93 pKa = 8.99 CTKK96 pKa = 10.45 EE97 pKa = 3.74 DD98 pKa = 3.69 TRR100 pKa = 11.84 FDD102 pKa = 4.11 GPWEE106 pKa = 4.03 FGKK109 pKa = 10.72 CLEE112 pKa = 4.49 PGQRR116 pKa = 11.84 RR117 pKa = 11.84 GYY119 pKa = 10.3 DD120 pKa = 3.16 EE121 pKa = 5.59 AVAAVCSGRR130 pKa = 11.84 CLAEE134 pKa = 3.96 VASEE138 pKa = 4.26 YY139 pKa = 9.17 PQVWVKK145 pKa = 10.29 YY146 pKa = 10.19 GRR148 pKa = 11.84 GLVDD152 pKa = 3.63 LRR154 pKa = 11.84 KK155 pKa = 9.42 QLRR158 pKa = 11.84 LDD160 pKa = 3.15 SDD162 pKa = 3.48 RR163 pKa = 11.84 RR164 pKa = 11.84 QFDD167 pKa = 3.93 DD168 pKa = 4.38 NGPEE172 pKa = 4.04 LWIFWGPSGTGKK184 pKa = 10.15 SKK186 pKa = 10.58 RR187 pKa = 11.84 ANEE190 pKa = 4.23 TWPDD194 pKa = 3.98 AYY196 pKa = 9.82 WKK198 pKa = 10.6 IPGEE202 pKa = 4.2 KK203 pKa = 8.69 WWDD206 pKa = 3.48 GYY208 pKa = 9.06 TNQDD212 pKa = 3.14 TVILDD217 pKa = 3.66 DD218 pKa = 4.17 FKK220 pKa = 11.6 GSFMRR225 pKa = 11.84 LTDD228 pKa = 3.77 FQRR231 pKa = 11.84 LIDD234 pKa = 5.33 RR235 pKa = 11.84 YY236 pKa = 8.75 PLWVEE241 pKa = 4.05 VKK243 pKa = 10.43 GGSIPMLATRR253 pKa = 11.84 YY254 pKa = 10.2 VITSNEE260 pKa = 3.91 APEE263 pKa = 4.02 AWYY266 pKa = 10.39 ASADD270 pKa = 3.35 PHH272 pKa = 5.4 GTVTRR277 pKa = 11.84 RR278 pKa = 11.84 CNDD281 pKa = 2.82 FTDD284 pKa = 3.69 GGRR287 pKa = 11.84 RR288 pKa = 11.84 IIYY291 pKa = 9.55 IGPEE295 pKa = 3.89 RR296 pKa = 11.84 QEE298 pKa = 4.64 LPDD301 pKa = 3.73 FEE303 pKa = 5.84 SWVGWGEE310 pKa = 3.85 FARR313 pKa = 4.7
Molecular weight: 36.5 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 6.056
IPC2_protein 6.059
IPC_protein 6.135
Toseland 6.351
ProMoST 6.517
Dawson 6.44
Bjellqvist 6.415
Wikipedia 6.44
Rodwell 6.427
Grimsley 6.453
Solomon 6.44
Lehninger 6.44
Nozaki 6.693
DTASelect 6.868
Thurlkill 6.883
EMBOSS 6.868
Sillero 6.81
Patrickios 4.342
IPC_peptide 6.453
IPC2_peptide 6.707
IPC2.peptide.svr19 6.676
Protein with the highest isoelectric point:
>tr|G1C9H6|G1C9H6_9VIRU ATP-dependent helicase Rep OS=Rodent stool-associated circular genome virus OX=1074214 PE=3 SV=1
MM1 pKa = 7.64 CVRR4 pKa = 11.84 IKK6 pKa = 8.29 MTQATWWLGTKK17 pKa = 9.83 FVHH20 pKa = 6.32 EE21 pKa = 4.14 AHH23 pKa = 6.84 DD24 pKa = 3.79 TEE26 pKa = 4.15 EE27 pKa = 3.96 WMRR30 pKa = 11.84 DD31 pKa = 3.11 LAEE34 pKa = 4.24 SGKK37 pKa = 10.62 VKK39 pKa = 10.7 FITGQRR45 pKa = 11.84 EE46 pKa = 4.14 LCPEE50 pKa = 4.37 TMRR53 pKa = 11.84 HH54 pKa = 5.02 HH55 pKa = 5.8 VQFVVNLHH63 pKa = 6.06 RR64 pKa = 11.84 SQRR67 pKa = 11.84 LSYY70 pKa = 10.2 LKK72 pKa = 10.62 KK73 pKa = 9.93 IDD75 pKa = 5.13 GEE77 pKa = 4.38 AHH79 pKa = 5.71 WEE81 pKa = 4.37 SVRR84 pKa = 11.84 GTPKK88 pKa = 10.09 QARR91 pKa = 11.84 DD92 pKa = 3.51 YY93 pKa = 8.99 CTKK96 pKa = 10.45 EE97 pKa = 3.74 DD98 pKa = 3.69 TRR100 pKa = 11.84 FDD102 pKa = 4.11 GPWEE106 pKa = 4.03 FGKK109 pKa = 10.72 CLEE112 pKa = 4.49 PGQRR116 pKa = 11.84 RR117 pKa = 11.84 GYY119 pKa = 10.3 DD120 pKa = 3.16 EE121 pKa = 5.59 AVAAVCSGRR130 pKa = 11.84 CLAEE134 pKa = 3.96 VASEE138 pKa = 4.26 YY139 pKa = 9.17 PQVWVKK145 pKa = 10.29 YY146 pKa = 10.19 GRR148 pKa = 11.84 GLVDD152 pKa = 3.63 LRR154 pKa = 11.84 KK155 pKa = 9.42 QLRR158 pKa = 11.84 LDD160 pKa = 3.15 SDD162 pKa = 3.48 RR163 pKa = 11.84 RR164 pKa = 11.84 QFDD167 pKa = 3.93 DD168 pKa = 4.38 NGPEE172 pKa = 4.04 LWIFWGPSGTGKK184 pKa = 10.15 SKK186 pKa = 10.58 RR187 pKa = 11.84 ANEE190 pKa = 4.23 TWPDD194 pKa = 3.98 AYY196 pKa = 9.82 WKK198 pKa = 10.6 IPGEE202 pKa = 4.2 KK203 pKa = 8.69 WWDD206 pKa = 3.48 GYY208 pKa = 9.06 TNQDD212 pKa = 3.14 TVILDD217 pKa = 3.66 DD218 pKa = 4.17 FKK220 pKa = 11.6 GSFMRR225 pKa = 11.84 LTDD228 pKa = 3.77 FQRR231 pKa = 11.84 LIDD234 pKa = 5.33 RR235 pKa = 11.84 YY236 pKa = 8.75 PLWVEE241 pKa = 4.05 VKK243 pKa = 10.43 GGSIPMLATRR253 pKa = 11.84 YY254 pKa = 10.2 VITSNEE260 pKa = 3.91 APEE263 pKa = 4.02 AWYY266 pKa = 10.39 ASADD270 pKa = 3.35 PHH272 pKa = 5.4 GTVTRR277 pKa = 11.84 RR278 pKa = 11.84 CNDD281 pKa = 2.82 FTDD284 pKa = 3.69 GGRR287 pKa = 11.84 RR288 pKa = 11.84 IIYY291 pKa = 9.55 IGPEE295 pKa = 3.89 RR296 pKa = 11.84 QEE298 pKa = 4.64 LPDD301 pKa = 3.73 FEE303 pKa = 5.84 SWVGWGEE310 pKa = 3.85 FARR313 pKa = 4.7
Molecular weight: 36.5 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 6.056
IPC2_protein 6.059
IPC_protein 6.135
Toseland 6.351
ProMoST 6.517
Dawson 6.44
Bjellqvist 6.415
Wikipedia 6.44
Rodwell 6.427
Grimsley 6.453
Solomon 6.44
Lehninger 6.44
Nozaki 6.693
DTASelect 6.868
Thurlkill 6.883
EMBOSS 6.868
Sillero 6.81
Patrickios 4.342
IPC_peptide 6.453
IPC2_peptide 6.707
IPC2.peptide.svr19 6.676
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
1
0
1
313
313
313
313.0
36.5
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
5.751 ± 0.0
2.236 ± 0.0
7.348 ± 0.0
7.668 ± 0.0
4.153 ± 0.0
8.626 ± 0.0
2.236 ± 0.0
3.834 ± 0.0
5.431 ± 0.0
6.07 ± 0.0
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
1.917 ± 0.0
1.917 ± 0.0
4.792 ± 0.0
3.834 ± 0.0
8.946 ± 0.0
4.473 ± 0.0
6.07 ± 0.0
6.07 ± 0.0
5.112 ± 0.0
3.514 ± 0.0
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here