Staphylococcus phage phiSP119-2

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; unclassified Siphoviridae

Average proteome isoelectric point is 6.39

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 63 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A499SJT4|A0A499SJT4_9CAUD Uncharacterized protein OS=Staphylococcus phage phiSP119-2 OX=2491317 PE=4 SV=1
MM1 pKa = 7.65KK2 pKa = 10.4YY3 pKa = 10.17IIAALIILYY12 pKa = 9.22VAHH15 pKa = 6.95DD16 pKa = 3.48TYY18 pKa = 11.38IRR20 pKa = 11.84LTANDD25 pKa = 4.96EE26 pKa = 3.5IDD28 pKa = 3.55QYY30 pKa = 11.56DD31 pKa = 3.8FRR33 pKa = 11.84AEE35 pKa = 3.81VSEE38 pKa = 4.18

Molecular weight: 4.48 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A499SJJ7|A0A499SJJ7_9CAUD Uncharacterized protein OS=Staphylococcus phage phiSP119-2 OX=2491317 PE=4 SV=1
MM1 pKa = 7.61IDD3 pKa = 3.29KK4 pKa = 10.32AQVKK8 pKa = 10.29AYY10 pKa = 7.54MKK12 pKa = 10.46QFFGSKK18 pKa = 9.78RR19 pKa = 11.84YY20 pKa = 9.65LYY22 pKa = 10.36QNGRR26 pKa = 11.84KK27 pKa = 8.64VAHH30 pKa = 5.46IHH32 pKa = 5.42IVNGIYY38 pKa = 10.22HH39 pKa = 5.87FHH41 pKa = 6.04GHH43 pKa = 6.41FKK45 pKa = 10.61TKK47 pKa = 10.36FSRR50 pKa = 11.84LKK52 pKa = 11.15LEE54 pKa = 4.93FKK56 pKa = 10.69CKK58 pKa = 9.86QDD60 pKa = 3.45FNDD63 pKa = 3.49YY64 pKa = 10.85LEE66 pKa = 4.14KK67 pKa = 10.9HH68 pKa = 5.44EE69 pKa = 5.51LRR71 pKa = 11.84FKK73 pKa = 10.6EE74 pKa = 4.14

Molecular weight: 9.11 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

63

0

63

12552

37

1861

199.2

22.7

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.095 ± 0.555

0.55 ± 0.107

6.405 ± 0.253

8.174 ± 0.414

4.191 ± 0.287

5.617 ± 0.572

1.84 ± 0.127

7.457 ± 0.308

9.855 ± 0.423

8.301 ± 0.256

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.47 ± 0.201

6.501 ± 0.289

2.438 ± 0.24

3.426 ± 0.27

3.721 ± 0.186

6.119 ± 0.239

5.88 ± 0.31

6.174 ± 0.361

0.94 ± 0.122

3.848 ± 0.263
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski