Allobacillus sp. SKP2-8

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Firmicutes; Bacilli; Bacillales; Bacillaceae; Allobacillus; unclassified Allobacillus

Average proteome isoelectric point is 5.99

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2475 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A556PNV1|A0A556PNV1_9BACI 2-keto-3-deoxygluconate permease OS=Allobacillus sp. SKP2-8 OX=1955271 GN=FPQ10_07940 PE=3 SV=1
MM1 pKa = 7.8LFFLSLALLLVIAACGGSDD20 pKa = 3.77EE21 pKa = 5.21DD22 pKa = 4.37SSSGSEE28 pKa = 3.93GEE30 pKa = 4.28EE31 pKa = 3.82NGEE34 pKa = 4.16EE35 pKa = 4.16SSSEE39 pKa = 4.0SSSEE43 pKa = 3.94DD44 pKa = 3.56GEE46 pKa = 4.29SSEE49 pKa = 5.32ASGEE53 pKa = 4.27SYY55 pKa = 10.67EE56 pKa = 5.56LEE58 pKa = 4.66FGMQPNTQANEE69 pKa = 3.72YY70 pKa = 9.73KK71 pKa = 10.54AAEE74 pKa = 4.0FLADD78 pKa = 3.84YY79 pKa = 10.75VEE81 pKa = 4.62KK82 pKa = 10.82EE83 pKa = 3.59SDD85 pKa = 3.21GRR87 pKa = 11.84LTVTIFPDD95 pKa = 3.4AQLGNDD101 pKa = 3.72LSMLGQLQDD110 pKa = 3.33GTLDD114 pKa = 3.11ITLAEE119 pKa = 4.11MGRR122 pKa = 11.84FGEE125 pKa = 4.39WIPRR129 pKa = 11.84AEE131 pKa = 4.58LLAMPYY137 pKa = 10.2VIEE140 pKa = 5.34DD141 pKa = 3.25FDD143 pKa = 4.56HH144 pKa = 6.66IKK146 pKa = 10.78NVVYY150 pKa = 8.91NTEE153 pKa = 3.86FGEE156 pKa = 4.16EE157 pKa = 3.65LRR159 pKa = 11.84AEE161 pKa = 4.18LVEE164 pKa = 4.01EE165 pKa = 3.97HH166 pKa = 6.3GMRR169 pKa = 11.84VIDD172 pKa = 3.68SAYY175 pKa = 9.61NGPRR179 pKa = 11.84VTSSNSPIEE188 pKa = 4.13EE189 pKa = 4.13LADD192 pKa = 3.59MEE194 pKa = 4.5GMSLRR199 pKa = 11.84VPEE202 pKa = 4.53AQTLLDD208 pKa = 3.57YY209 pKa = 11.59AEE211 pKa = 4.14YY212 pKa = 9.94TGANPTPMAFGEE224 pKa = 4.67VYY226 pKa = 10.6LALQTGQVDD235 pKa = 4.05GQEE238 pKa = 4.28NPLPTIEE245 pKa = 4.28AQSFHH250 pKa = 6.53EE251 pKa = 4.23VQDD254 pKa = 4.21YY255 pKa = 10.78IALTNHH261 pKa = 5.12VVNDD265 pKa = 3.33NTYY268 pKa = 8.8VVSEE272 pKa = 4.4KK273 pKa = 10.12TYY275 pKa = 10.81QDD277 pKa = 3.44LPEE280 pKa = 4.63DD281 pKa = 3.39LRR283 pKa = 11.84TILEE287 pKa = 4.12EE288 pKa = 5.18GIDD291 pKa = 3.7AATDD295 pKa = 3.05HH296 pKa = 6.24HH297 pKa = 6.9TEE299 pKa = 4.2LFQTQEE305 pKa = 4.08DD306 pKa = 4.19EE307 pKa = 4.69LISFFEE313 pKa = 4.33EE314 pKa = 3.71EE315 pKa = 4.79GVTITEE321 pKa = 4.21PNLDD325 pKa = 3.82EE326 pKa = 4.47FRR328 pKa = 11.84EE329 pKa = 4.63AVSEE333 pKa = 4.36AYY335 pKa = 9.39PSYY338 pKa = 11.16YY339 pKa = 10.45EE340 pKa = 5.98DD341 pKa = 3.2IGEE344 pKa = 4.24DD345 pKa = 3.16AEE347 pKa = 5.15KK348 pKa = 10.76YY349 pKa = 9.51MEE351 pKa = 4.7VIQGAAEE358 pKa = 3.79

Molecular weight: 39.65 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A556PMV2|A0A556PMV2_9BACI TIGR02678 family protein OS=Allobacillus sp. SKP2-8 OX=1955271 GN=FPQ10_08795 PE=4 SV=1
MM1 pKa = 7.36KK2 pKa = 9.43RR3 pKa = 11.84TYY5 pKa = 9.54QPKK8 pKa = 9.44KK9 pKa = 7.96RR10 pKa = 11.84KK11 pKa = 8.69HH12 pKa = 5.94SKK14 pKa = 8.54VHH16 pKa = 5.76GFRR19 pKa = 11.84ARR21 pKa = 11.84MKK23 pKa = 8.78TKK25 pKa = 10.39NGRR28 pKa = 11.84KK29 pKa = 8.66VLARR33 pKa = 11.84RR34 pKa = 11.84RR35 pKa = 11.84KK36 pKa = 9.2KK37 pKa = 10.05GRR39 pKa = 11.84KK40 pKa = 8.66VLSAA44 pKa = 4.05

Molecular weight: 5.3 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2475

0

2475

700000

25

1429

282.8

31.93

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.523 ± 0.047

0.562 ± 0.014

5.59 ± 0.042

8.287 ± 0.062

4.605 ± 0.043

6.639 ± 0.047

2.129 ± 0.024

7.712 ± 0.049

6.461 ± 0.048

9.538 ± 0.052

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.848 ± 0.026

4.398 ± 0.033

3.525 ± 0.031

4.221 ± 0.043

4.191 ± 0.035

5.89 ± 0.035

5.401 ± 0.033

7.021 ± 0.04

0.961 ± 0.017

3.498 ± 0.034
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski