Gordonia phage Dmitri

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; unclassified Siphoviridae

Average proteome isoelectric point is 6.28

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 63 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A514DFV6|A0A514DFV6_9CAUD Uncharacterized protein OS=Gordonia phage Dmitri OX=2591207 GN=39 PE=4 SV=1
MM1 pKa = 7.34NPNHH5 pKa = 6.68DD6 pKa = 4.09HH7 pKa = 7.18DD8 pKa = 4.01HH9 pKa = 7.05RR10 pKa = 11.84EE11 pKa = 3.69YY12 pKa = 11.55DD13 pKa = 4.85DD14 pKa = 3.89IDD16 pKa = 3.96RR17 pKa = 11.84ADD19 pKa = 3.26WAADD23 pKa = 3.32SEE25 pKa = 5.18GPVMYY30 pKa = 10.0PPDD33 pKa = 3.74HH34 pKa = 6.77VGPDD38 pKa = 3.69EE39 pKa = 6.2DD40 pKa = 5.78DD41 pKa = 3.29GTMPDD46 pKa = 3.26NVADD50 pKa = 4.47LRR52 pKa = 11.84DD53 pKa = 3.53AVIGRR58 pKa = 11.84RR59 pKa = 11.84IVSAEE64 pKa = 3.73QTDD67 pKa = 4.15FRR69 pKa = 11.84AADD72 pKa = 4.39FAASEE77 pKa = 4.28HH78 pKa = 4.81TAYY81 pKa = 9.74WSSLDD86 pKa = 3.59GNGLVLTLDD95 pKa = 3.67DD96 pKa = 4.0GRR98 pKa = 11.84RR99 pKa = 11.84VALVDD104 pKa = 3.11TGYY107 pKa = 10.95CCAYY111 pKa = 9.74TDD113 pKa = 3.89LQEE116 pKa = 5.16FFLNPDD122 pKa = 3.85LVDD125 pKa = 3.52HH126 pKa = 7.32AITGVATTDD135 pKa = 4.75GYY137 pKa = 6.7TTWHH141 pKa = 6.94IYY143 pKa = 11.07ADD145 pKa = 3.92LGDD148 pKa = 3.57IMRR151 pKa = 11.84LKK153 pKa = 10.83VGWSAGNPFYY163 pKa = 10.89YY164 pKa = 10.41GYY166 pKa = 10.91GFDD169 pKa = 4.0IGVSTTIEE177 pKa = 4.05GSLALPEE184 pKa = 4.32IEE186 pKa = 5.3GPSHH190 pKa = 7.46DD191 pKa = 3.78

Molecular weight: 21.02 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A514DFW4|A0A514DFW4_9CAUD Uncharacterized protein OS=Gordonia phage Dmitri OX=2591207 GN=61 PE=4 SV=1
MM1 pKa = 7.32QSTGVLTPEE10 pKa = 4.82GVDD13 pKa = 3.48SLITGAEE20 pKa = 4.01AARR23 pKa = 11.84LCGVSTVTIRR33 pKa = 11.84KK34 pKa = 4.84WTHH37 pKa = 5.71RR38 pKa = 11.84GYY40 pKa = 10.19IDD42 pKa = 4.66SKK44 pKa = 11.27GAGQKK49 pKa = 10.69LEE51 pKa = 3.88VAGRR55 pKa = 11.84DD56 pKa = 3.3RR57 pKa = 11.84QGRR60 pKa = 11.84NLYY63 pKa = 10.44RR64 pKa = 11.84LIDD67 pKa = 3.65VAKK70 pKa = 10.46AEE72 pKa = 4.16HH73 pKa = 6.17ATRR76 pKa = 11.84ARR78 pKa = 11.84ARR80 pKa = 11.84RR81 pKa = 11.84SS82 pKa = 3.13

Molecular weight: 8.94 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

63

0

63

14878

50

1896

236.2

25.5

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

12.777 ± 0.605

0.807 ± 0.139

6.802 ± 0.335

5.626 ± 0.312

2.507 ± 0.181

8.657 ± 0.439

1.909 ± 0.16

4.228 ± 0.21

3.159 ± 0.204

8.341 ± 0.269

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.131 ± 0.112

2.554 ± 0.143

5.552 ± 0.37

3.824 ± 0.306

7.071 ± 0.36

5.841 ± 0.241

6.842 ± 0.249

7.279 ± 0.274

1.949 ± 0.178

2.144 ± 0.137
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski