Lactococcus virus bIL67

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; Ceduovirus

Average proteome isoelectric point is 6.33

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 37 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|Q38244|Q38244_9CAUD Uncharacterized protein OS=Lactococcus virus bIL67 OX=36343 PE=4 SV=1
MM1 pKa = 7.66AEE3 pKa = 3.91KK4 pKa = 10.59NIYY7 pKa = 9.18FVNDD11 pKa = 3.22EE12 pKa = 4.52TEE14 pKa = 4.11LKK16 pKa = 10.3QVLEE20 pKa = 5.32FIDD23 pKa = 3.55KK24 pKa = 9.73TDD26 pKa = 3.33YY27 pKa = 11.28GINIDD32 pKa = 3.63KK33 pKa = 10.06TRR35 pKa = 11.84EE36 pKa = 3.6DD37 pKa = 3.69VYY39 pKa = 11.64AVVTSYY45 pKa = 11.55SLPII49 pKa = 4.27

Molecular weight: 5.73 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|Q38236|Q38236_9CAUD Uncharacterized protein OS=Lactococcus virus bIL67 OX=36343 PE=4 SV=1
MM1 pKa = 7.52ATTKK5 pKa = 10.43QVTGLEE11 pKa = 4.28KK12 pKa = 10.13FTEE15 pKa = 4.18KK16 pKa = 10.32QLKK19 pKa = 9.56KK20 pKa = 10.6VWLEE24 pKa = 3.57MADD27 pKa = 3.65AFNSNQNTVKK37 pKa = 10.51RR38 pKa = 11.84SYY40 pKa = 10.61KK41 pKa = 10.29SSLGGDD47 pKa = 3.82FSGYY51 pKa = 8.69RR52 pKa = 11.84AKK54 pKa = 10.7FDD56 pKa = 3.46TKK58 pKa = 10.74KK59 pKa = 8.9ITKK62 pKa = 9.39QVTRR66 pKa = 11.84SYY68 pKa = 11.74GSLKK72 pKa = 10.39SGNIGIINGFKK83 pKa = 10.79AKK85 pKa = 10.43DD86 pKa = 3.64EE87 pKa = 4.19SWRR90 pKa = 11.84MLNVLLHH97 pKa = 6.71DD98 pKa = 3.74RR99 pKa = 11.84HH100 pKa = 5.47LHH102 pKa = 4.35QRR104 pKa = 11.84YY105 pKa = 8.47GQTLIKK111 pKa = 9.46ATHH114 pKa = 5.95EE115 pKa = 4.16MDD117 pKa = 5.74DD118 pKa = 3.35KK119 pKa = 11.4TKK121 pKa = 9.88TIKK124 pKa = 10.41RR125 pKa = 11.84KK126 pKa = 9.57LRR128 pKa = 11.84SITNNGG134 pKa = 2.99

Molecular weight: 15.38 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

37

0

37

6610

41

628

178.6

20.33

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.067 ± 0.447

0.726 ± 0.153

6.006 ± 0.312

7.398 ± 0.568

4.493 ± 0.283

6.899 ± 0.455

1.377 ± 0.153

6.278 ± 0.451

8.896 ± 0.563

8.563 ± 0.372

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.874 ± 0.195

6.415 ± 0.345

2.421 ± 0.332

3.828 ± 0.387

3.631 ± 0.287

5.825 ± 0.425

6.218 ± 0.389

6.097 ± 0.377

1.452 ± 0.268

4.539 ± 0.392
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski