Defluviimonas alba
Average proteome isoelectric point is 6.62
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 5035 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A159Z2E0|A0A159Z2E0_9RHOB Uncharacterized protein OS=Defluviimonas alba OX=1335048 GN=AKL17_1892 PE=4 SV=1
MM1 pKa = 7.71 SSRR4 pKa = 11.84 FLLPLAVAGAFCAASVLPAAALDD27 pKa = 3.44 RR28 pKa = 11.84 HH29 pKa = 5.35 VRR31 pKa = 11.84 IVNDD35 pKa = 3.22 TGFAIVEE42 pKa = 5.02 FYY44 pKa = 11.31 ASNTGTSDD52 pKa = 2.88 WQEE55 pKa = 4.79 DD56 pKa = 3.58 ILGPDD61 pKa = 3.84 VLPSGASVMINIDD74 pKa = 4.5 DD75 pKa = 3.74 GTGYY79 pKa = 10.68 CKK81 pKa = 10.55 YY82 pKa = 10.8 DD83 pKa = 3.64 LLAVFEE89 pKa = 5.99 DD90 pKa = 4.11 GDD92 pKa = 3.9 QLVKK96 pKa = 10.77 SDD98 pKa = 3.92 VNVCEE103 pKa = 4.43 VGTFTYY109 pKa = 10.96 NN110 pKa = 2.81
Molecular weight: 11.73 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.731
IPC2_protein 3.872
IPC_protein 3.834
Toseland 3.617
ProMoST 4.024
Dawson 3.846
Bjellqvist 3.999
Wikipedia 3.821
Rodwell 3.668
Grimsley 3.528
Solomon 3.821
Lehninger 3.783
Nozaki 3.973
DTASelect 4.228
Thurlkill 3.694
EMBOSS 3.821
Sillero 3.961
Patrickios 0.846
IPC_peptide 3.821
IPC2_peptide 3.935
IPC2.peptide.svr19 3.848
Protein with the highest isoelectric point:
>tr|A0A161HCN6|A0A161HCN6_9RHOB GntR family transcriptional regulator OS=Defluviimonas alba OX=1335048 GN=AKL17_3625 PE=4 SV=1
MM1 pKa = 7.49 HH2 pKa = 7.64 PASLVIATAALALLAALSLAANRR25 pKa = 11.84 RR26 pKa = 11.84 LRR28 pKa = 11.84 HH29 pKa = 5.77 LGRR32 pKa = 11.84 LPMHH36 pKa = 7.08 WGLAGQVTWTAPRR49 pKa = 11.84 PLALALVPILAAIMLLASALSGAPPLMTALMALALIAAHH88 pKa = 7.32 LAHH91 pKa = 6.99 LWLIRR96 pKa = 11.84 RR97 pKa = 11.84 TALRR101 pKa = 11.84 GTPP104 pKa = 3.35
Molecular weight: 10.9 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.485
IPC2_protein 11.067
IPC_protein 12.691
Toseland 12.852
ProMoST 13.349
Dawson 12.852
Bjellqvist 12.852
Wikipedia 13.32
Rodwell 12.34
Grimsley 12.881
Solomon 13.349
Lehninger 13.247
Nozaki 12.852
DTASelect 12.852
Thurlkill 12.852
EMBOSS 13.349
Sillero 12.852
Patrickios 12.106
IPC_peptide 13.349
IPC2_peptide 12.34
IPC2.peptide.svr19 9.163
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
5035
0
5035
1461988
30
4091
290.4
31.25
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
13.877 ± 0.057
0.902 ± 0.012
5.449 ± 0.033
5.516 ± 0.034
3.511 ± 0.023
9.161 ± 0.056
2.024 ± 0.019
4.768 ± 0.024
2.547 ± 0.027
10.517 ± 0.042
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.696 ± 0.017
2.157 ± 0.018
5.573 ± 0.029
3.056 ± 0.018
7.444 ± 0.039
4.882 ± 0.025
5.237 ± 0.029
7.233 ± 0.031
1.432 ± 0.016
2.019 ± 0.018
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here