Wuhan insect virus 35

Taxonomy: Viruses; Riboviria; unclassified Riboviria; unclassified RNA viruses ShiM-2016

Average proteome isoelectric point is 7.63

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1L3KGP0|A0A1L3KGP0_9VIRU DiSB-ORF2_chro domain-containing protein OS=Wuhan insect virus 35 OX=1923738 PE=4 SV=1
MM1 pKa = 8.3DD2 pKa = 4.35YY3 pKa = 10.97LRR5 pKa = 11.84QLMQNFIGGEE15 pKa = 3.86PDD17 pKa = 3.35EE18 pKa = 5.35LPEE21 pKa = 4.08EE22 pKa = 4.17QAVEE26 pKa = 4.45RR27 pKa = 11.84EE28 pKa = 4.2TQLDD32 pKa = 3.76QIINTLDD39 pKa = 3.14RR40 pKa = 11.84LRR42 pKa = 11.84RR43 pKa = 11.84VQQLTHH49 pKa = 6.9EE50 pKa = 4.52IDD52 pKa = 3.95DD53 pKa = 4.29ARR55 pKa = 11.84TAPIPQGEE63 pKa = 4.33TAEE66 pKa = 4.06EE67 pKa = 3.93RR68 pKa = 11.84VLRR71 pKa = 11.84GVRR74 pKa = 11.84AQAEE78 pKa = 4.76VIEE81 pKa = 4.26HH82 pKa = 5.94RR83 pKa = 11.84QVIDD87 pKa = 4.68DD88 pKa = 3.8KK89 pKa = 9.5TTPPSVDD96 pKa = 2.93DD97 pKa = 4.65CYY99 pKa = 11.85AFLKK103 pKa = 9.0TRR105 pKa = 11.84CEE107 pKa = 4.01GKK109 pKa = 10.63LVDD112 pKa = 3.9GTYY115 pKa = 10.69NAYY118 pKa = 9.93CAALIRR124 pKa = 11.84QYY126 pKa = 10.9FATRR130 pKa = 11.84KK131 pKa = 8.39PVSNKK136 pKa = 8.8MEE138 pKa = 4.08YY139 pKa = 10.69AIMNSVMNMHH149 pKa = 7.87LINIRR154 pKa = 11.84PQYY157 pKa = 11.02DD158 pKa = 3.62YY159 pKa = 11.3DD160 pKa = 3.53LHH162 pKa = 7.74DD163 pKa = 3.59ILRR166 pKa = 11.84YY167 pKa = 9.33NDD169 pKa = 4.15CINYY173 pKa = 7.71VQRR176 pKa = 11.84KK177 pKa = 5.69TIRR180 pKa = 11.84IFNQIIEE187 pKa = 4.26IPFTRR192 pKa = 11.84ITLEE196 pKa = 3.97DD197 pKa = 3.85LNIFRR202 pKa = 11.84PAATSRR208 pKa = 11.84PINFLWKK215 pKa = 10.48LFLALSLIIIAYY227 pKa = 7.95YY228 pKa = 9.98AVHH231 pKa = 7.42FITLPFTLLTTFTTTHH247 pKa = 6.15NQPQQPSQPIITATDD262 pKa = 3.41PSYY265 pKa = 11.14YY266 pKa = 9.04QTITHH271 pKa = 7.68LISTWTTSSTSSNDD285 pKa = 3.72DD286 pKa = 3.51YY287 pKa = 12.11SHH289 pKa = 6.94GPLSSIYY296 pKa = 9.36HH297 pKa = 4.74QWIAPASEE305 pKa = 4.32SFTRR309 pKa = 11.84TLTLVYY315 pKa = 10.8YY316 pKa = 10.63KK317 pKa = 10.22DD318 pKa = 3.62AKK320 pKa = 10.0PALEE324 pKa = 4.3SLLSLSWRR332 pKa = 11.84KK333 pKa = 8.65CQHH336 pKa = 6.65LNTKK340 pKa = 10.08LQDD343 pKa = 3.57LYY345 pKa = 11.63KK346 pKa = 10.63EE347 pKa = 4.45GTPHH351 pKa = 6.53LTYY354 pKa = 11.47AMDD357 pKa = 5.76AISNHH362 pKa = 4.82WNAVSKK368 pKa = 10.21ATDD371 pKa = 3.35TLAKK375 pKa = 10.09EE376 pKa = 4.18LTTILAAKK384 pKa = 7.4YY385 pKa = 8.15TSSLANINTTLRR397 pKa = 11.84EE398 pKa = 4.12TILPLTLTSLSKK410 pKa = 10.97CYY412 pKa = 10.49DD413 pKa = 3.39YY414 pKa = 11.06ATDD417 pKa = 4.62FIYY420 pKa = 10.74DD421 pKa = 3.78VTNKK425 pKa = 10.08APNYY429 pKa = 9.04LSSVKK434 pKa = 10.25KK435 pKa = 10.33QLEE438 pKa = 4.52TEE440 pKa = 4.62LLPAMEE446 pKa = 5.44KK447 pKa = 10.29SIQYY451 pKa = 9.65AAQEE455 pKa = 4.46CPAMWTQALEE465 pKa = 4.07IASSIITSSGMLLNDD480 pKa = 5.19FISTVMRR487 pKa = 11.84LSTEE491 pKa = 4.36TISSYY496 pKa = 11.24SQTLKK501 pKa = 10.73SQQQNSQTYY510 pKa = 8.47YY511 pKa = 11.19GSTIWKK517 pKa = 7.89QLLVIVLILYY527 pKa = 7.62TKK529 pKa = 10.91LNFAVADD536 pKa = 3.54WFIIPTAIPQWPLTRR551 pKa = 11.84IDD553 pKa = 3.22

Molecular weight: 63.36 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1L3KH03|A0A1L3KH03_9VIRU RNA-directed RNA polymerase OS=Wuhan insect virus 35 OX=1923738 PE=4 SV=1
MM1 pKa = 6.87EE2 pKa = 4.95TVPGIITNHH11 pKa = 4.84NRR13 pKa = 11.84LLRR16 pKa = 11.84RR17 pKa = 11.84TLYY20 pKa = 10.36HH21 pKa = 6.13PPIYY25 pKa = 10.3APHH28 pKa = 7.12DD29 pKa = 3.95FYY31 pKa = 11.79YY32 pKa = 10.17YY33 pKa = 10.46AQSATTTIAAYY44 pKa = 10.07HH45 pKa = 5.67NRR47 pKa = 11.84HH48 pKa = 5.94RR49 pKa = 11.84PIILPDD55 pKa = 3.48YY56 pKa = 10.25NPSHH60 pKa = 6.91FNVDD64 pKa = 3.53NIFDD68 pKa = 3.69KK69 pKa = 10.97FKK71 pKa = 11.02RR72 pKa = 11.84RR73 pKa = 11.84LQPWTFEE80 pKa = 4.14QYY82 pKa = 10.08ISSMDD87 pKa = 3.45SPRR90 pKa = 11.84KK91 pKa = 9.33RR92 pKa = 11.84KK93 pKa = 9.4FYY95 pKa = 10.56QDD97 pKa = 3.59AYY99 pKa = 9.98TSILQGRR106 pKa = 11.84KK107 pKa = 6.77TSSGITPFTKK117 pKa = 10.25LEE119 pKa = 3.97KK120 pKa = 10.05MSTSKK125 pKa = 11.12YY126 pKa = 9.02KK127 pKa = 10.62APRR130 pKa = 11.84LIQGRR135 pKa = 11.84HH136 pKa = 3.97PTFNLCYY143 pKa = 10.14GRR145 pKa = 11.84YY146 pKa = 8.84IKK148 pKa = 10.4PLEE151 pKa = 4.32RR152 pKa = 11.84SLKK155 pKa = 9.73GDD157 pKa = 3.28RR158 pKa = 11.84HH159 pKa = 5.76FGKK162 pKa = 9.03GTYY165 pKa = 10.34DD166 pKa = 3.93DD167 pKa = 4.1IGRR170 pKa = 11.84KK171 pKa = 7.15IHH173 pKa = 6.35KK174 pKa = 10.12LSRR177 pKa = 11.84KK178 pKa = 7.88YY179 pKa = 10.51KK180 pKa = 8.49YY181 pKa = 9.07YY182 pKa = 10.7TEE184 pKa = 5.02GDD186 pKa = 3.3HH187 pKa = 5.74TTFDD191 pKa = 3.49SHH193 pKa = 5.5VTVEE197 pKa = 4.04MLRR200 pKa = 11.84LCHH203 pKa = 6.7RR204 pKa = 11.84FYY206 pKa = 11.23LRR208 pKa = 11.84CYY210 pKa = 8.62KK211 pKa = 10.29QSPEE215 pKa = 4.27LLKK218 pKa = 10.88LCKK221 pKa = 9.35KK222 pKa = 8.68TIRR225 pKa = 11.84NRR227 pKa = 11.84AVARR231 pKa = 11.84NGEE234 pKa = 4.39KK235 pKa = 9.42YY236 pKa = 7.83TVRR239 pKa = 11.84GTRR242 pKa = 11.84MSGDD246 pKa = 3.12VDD248 pKa = 3.51TGFGNSIINYY258 pKa = 8.11YY259 pKa = 9.81IIRR262 pKa = 11.84HH263 pKa = 4.72ALKK266 pKa = 10.19RR267 pKa = 11.84LHH269 pKa = 6.9IDD271 pKa = 2.92GDD273 pKa = 4.42AIVNGDD279 pKa = 3.89DD280 pKa = 5.52FIIFTNIKK288 pKa = 9.62ISTAEE293 pKa = 3.74FTNLLRR299 pKa = 11.84EE300 pKa = 4.34YY301 pKa = 11.41NMEE304 pKa = 4.07TTLGNSVDD312 pKa = 4.58SIHH315 pKa = 6.22QVEE318 pKa = 5.49FCRR321 pKa = 11.84CRR323 pKa = 11.84LVYY326 pKa = 10.5HH327 pKa = 7.16PDD329 pKa = 3.02GHH331 pKa = 6.34PTMAFDD337 pKa = 3.46PHH339 pKa = 8.13RR340 pKa = 11.84LEE342 pKa = 5.61KK343 pKa = 10.19IYY345 pKa = 10.86GCSNILYY352 pKa = 7.75PKK354 pKa = 8.93KK355 pKa = 10.35KK356 pKa = 10.21KK357 pKa = 10.82YY358 pKa = 10.31EE359 pKa = 4.49DD360 pKa = 3.6YY361 pKa = 11.25LKK363 pKa = 10.03TIQHH367 pKa = 6.55ANHH370 pKa = 6.98MINLNSPIHH379 pKa = 6.59NDD381 pKa = 2.91WQLLIDD387 pKa = 5.67DD388 pKa = 4.4DD389 pKa = 4.46TALKK393 pKa = 11.02LMTPQLKK400 pKa = 9.91RR401 pKa = 11.84IYY403 pKa = 10.05HH404 pKa = 5.45KK405 pKa = 9.64QHH407 pKa = 6.32SNRR410 pKa = 11.84VYY412 pKa = 8.5TWDD415 pKa = 3.55YY416 pKa = 8.28LTPSFIQAYY425 pKa = 9.07PNYY428 pKa = 8.4KK429 pKa = 8.46TRR431 pKa = 11.84KK432 pKa = 9.31KK433 pKa = 9.92PFPLARR439 pKa = 11.84TIPQQRR445 pKa = 11.84QLLINHH451 pKa = 5.96NTRR454 pKa = 11.84EE455 pKa = 4.37LITLL459 pKa = 4.29

Molecular weight: 54.36 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3

0

3

1505

459

553

501.7

57.91

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

5.648 ± 0.908

1.927 ± 0.444

5.249 ± 0.173

3.522 ± 0.662

4.186 ± 0.677

3.056 ± 0.629

3.189 ± 0.775

7.708 ± 0.676

4.983 ± 0.99

9.568 ± 0.61

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.86 ± 0.093

4.85 ± 0.269

5.847 ± 0.594

4.718 ± 0.618

5.847 ± 0.869

7.442 ± 1.025

9.369 ± 0.647

3.787 ± 0.537

0.997 ± 0.291

6.246 ± 0.43
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski