Bat Middle East Hepe-Astrovirus

Taxonomy: Viruses; Riboviria; unclassified Riboviria; unclassified ssRNA viruses; unclassified ssRNA positive-strand viruses

Average proteome isoelectric point is 7.5

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A2U7N8C8|A0A2U7N8C8_9VIRU Capsid polyprotein VP90 OS=Bat Middle East Hepe-Astrovirus OX=2301732 PE=3 SV=1
MM1 pKa = 7.58RR2 pKa = 11.84VLFFRR7 pKa = 11.84GSPPMSMTSSACRR20 pKa = 11.84SAMDD24 pKa = 4.14LSYY27 pKa = 10.68PQGAEE32 pKa = 4.27PTWGTQIAAINAGAAARR49 pKa = 11.84RR50 pKa = 11.84VRR52 pKa = 11.84AALHH56 pKa = 5.15VPYY59 pKa = 10.85VLTQTQKK66 pKa = 9.17TRR68 pKa = 11.84FKK70 pKa = 11.22DD71 pKa = 3.57LVRR74 pKa = 11.84CDD76 pKa = 3.45VVFEE80 pKa = 4.47DD81 pKa = 4.97RR82 pKa = 11.84VEE84 pKa = 4.09HH85 pKa = 4.89THH87 pKa = 6.03AVLRR91 pKa = 11.84ALHH94 pKa = 5.79YY95 pKa = 10.24FADD98 pKa = 3.79ATAFRR103 pKa = 11.84HH104 pKa = 5.97ADD106 pKa = 3.49DD107 pKa = 5.22VIDD110 pKa = 4.16IGGDD114 pKa = 3.14PRR116 pKa = 11.84KK117 pKa = 10.51NKK119 pKa = 7.64TRR121 pKa = 11.84RR122 pKa = 11.84NHH124 pKa = 4.0VCMMVDD130 pKa = 3.3NARR133 pKa = 11.84DD134 pKa = 3.35EE135 pKa = 4.31LRR137 pKa = 11.84AMRR140 pKa = 11.84ANLDD144 pKa = 3.39PRR146 pKa = 11.84PDD148 pKa = 3.26VGTRR152 pKa = 11.84CYY154 pKa = 10.92GGAQWCNVQAPRR166 pKa = 11.84GVSIHH171 pKa = 5.83SAYY174 pKa = 10.6DD175 pKa = 3.21ITWEE179 pKa = 3.7VWEE182 pKa = 4.84QIFEE186 pKa = 4.15AHH188 pKa = 6.98GLQCVDD194 pKa = 2.79VWLLEE199 pKa = 4.0PEE201 pKa = 4.22EE202 pKa = 4.56LFGGTTDD209 pKa = 3.25AFYY212 pKa = 10.81GIQTTAVGQNHH223 pKa = 5.87VMLLDD228 pKa = 4.95DD229 pKa = 3.95GTAGYY234 pKa = 7.52EE235 pKa = 3.94HH236 pKa = 7.06RR237 pKa = 11.84TVEE240 pKa = 3.75WTKK243 pKa = 9.5YY244 pKa = 9.27RR245 pKa = 11.84LSNGHH250 pKa = 5.44VGKK253 pKa = 10.23NFNLAVLPEE262 pKa = 4.35GQWGILRR269 pKa = 11.84RR270 pKa = 11.84YY271 pKa = 8.28TIFRR275 pKa = 11.84SHH277 pKa = 6.55RR278 pKa = 11.84PARR281 pKa = 11.84IWQAKK286 pKa = 8.65CAVDD290 pKa = 3.77RR291 pKa = 11.84NTAHH295 pKa = 6.51VPTLAGGCIKK305 pKa = 10.63VPLQHH310 pKa = 6.98WDD312 pKa = 3.52NLLSWGLARR321 pKa = 11.84GDD323 pKa = 3.62EE324 pKa = 4.32KK325 pKa = 11.03FTFQALMAYY334 pKa = 9.8ARR336 pKa = 11.84ALRR339 pKa = 11.84HH340 pKa = 5.4RR341 pKa = 11.84LIVGSNVVHH350 pKa = 6.9GGWTQDD356 pKa = 2.75AVEE359 pKa = 4.06QHH361 pKa = 7.01DD362 pKa = 4.28VVQTAFVVCAALRR375 pKa = 11.84FMRR378 pKa = 11.84TQSLSLALRR387 pKa = 11.84RR388 pKa = 11.84LKK390 pKa = 10.72HH391 pKa = 4.61EE392 pKa = 4.22QGRR395 pKa = 11.84GWFGEE400 pKa = 4.11LIDD403 pKa = 5.74RR404 pKa = 11.84LKK406 pKa = 11.08AWLGIPAIRR415 pKa = 11.84LRR417 pKa = 11.84QFLVTDD423 pKa = 3.56LHH425 pKa = 6.06NLRR428 pKa = 11.84AGRR431 pKa = 11.84DD432 pKa = 3.45SAVPLLRR439 pKa = 11.84EE440 pKa = 3.75LPGRR444 pKa = 11.84VGGNFWPEE452 pKa = 3.54EE453 pKa = 4.11DD454 pKa = 3.68TDD456 pKa = 3.87EE457 pKa = 4.94GPEE460 pKa = 4.0FTPEE464 pKa = 3.75EE465 pKa = 4.27PLPPEE470 pKa = 4.13PAEE473 pKa = 3.74LLAYY477 pKa = 8.06RR478 pKa = 11.84TALQAQTCTAGPYY491 pKa = 10.38AEE493 pKa = 5.5LAQQTLDD500 pKa = 3.61FLDD503 pKa = 3.99AAEE506 pKa = 4.34CRR508 pKa = 11.84ARR510 pKa = 11.84PKK512 pKa = 9.69RR513 pKa = 11.84TLIEE517 pKa = 4.37GPPGSGKK524 pKa = 7.47STRR527 pKa = 11.84FADD530 pKa = 3.24WSDD533 pKa = 3.29DD534 pKa = 3.4RR535 pKa = 11.84KK536 pKa = 11.2ANTLVVVPTKK546 pKa = 10.29KK547 pKa = 10.29QKK549 pKa = 11.06ADD551 pKa = 2.74WAQRR555 pKa = 11.84GFTAHH560 pKa = 6.23TPQRR564 pKa = 11.84ALQQCVGYY572 pKa = 9.09PLVIVDD578 pKa = 4.43EE579 pKa = 4.62VSLVHH584 pKa = 6.21PGVVRR589 pKa = 11.84ALEE592 pKa = 4.12RR593 pKa = 11.84HH594 pKa = 5.73SGAAQLYY601 pKa = 9.55CVGDD605 pKa = 3.83LKK607 pKa = 11.18QIAFCDD613 pKa = 4.25FEE615 pKa = 4.55GLGVEE620 pKa = 4.91FDD622 pKa = 3.78LQRR625 pKa = 11.84FYY627 pKa = 11.77ADD629 pKa = 3.39WEE631 pKa = 4.55KK632 pKa = 10.36EE633 pKa = 4.38TLTVTHH639 pKa = 7.3RR640 pKa = 11.84CPVDD644 pKa = 3.33VTKK647 pKa = 11.07VLGRR651 pKa = 11.84YY652 pKa = 9.0YY653 pKa = 10.7EE654 pKa = 5.12GITTTNTRR662 pKa = 11.84QVSIIKK668 pKa = 8.96GVCNPAAQHH677 pKa = 6.06LCYY680 pKa = 9.83TQGTKK685 pKa = 10.38SKK687 pKa = 9.4LTSQHH692 pKa = 6.17PNVLTVHH699 pKa = 6.53EE700 pKa = 4.64AQGGTYY706 pKa = 10.2KK707 pKa = 10.54AVCLHH712 pKa = 6.45VEE714 pKa = 4.12AQDD717 pKa = 3.35ATLVRR722 pKa = 11.84TSRR725 pKa = 11.84AHH727 pKa = 5.73NLVAITRR734 pKa = 11.84HH735 pKa = 5.05TGSLVVCEE743 pKa = 4.41QGTTALSEE751 pKa = 4.06WLDD754 pKa = 3.38PAFAVCEE761 pKa = 3.92LAEE764 pKa = 4.61GLDD767 pKa = 3.91AAPVTVPEE775 pKa = 4.4DD776 pKa = 3.21ARR778 pKa = 11.84ATVSPADD785 pKa = 3.64EE786 pKa = 5.19AGNSIPPHH794 pKa = 5.76TGANLDD800 pKa = 3.86DD801 pKa = 5.06LGVTFGADD809 pKa = 2.93LGVEE813 pKa = 4.76RR814 pKa = 11.84ITAAALPAAQRR825 pKa = 11.84TAQIQLDD832 pKa = 4.26SFPTLDD838 pKa = 3.38VSKK841 pKa = 10.61LALTAVPAPACRR853 pKa = 11.84FSRR856 pKa = 11.84PQDD859 pKa = 3.19KK860 pKa = 10.45AAAAAAVLSRR870 pKa = 11.84ITGTNKK876 pKa = 9.83NCGKK880 pKa = 10.2QEE882 pKa = 3.92AKK884 pKa = 10.62VRR886 pKa = 11.84GAALRR891 pKa = 11.84RR892 pKa = 11.84AFDD895 pKa = 3.15QWLEE899 pKa = 4.05PEE901 pKa = 4.55DD902 pKa = 4.67AVVSPDD908 pKa = 3.35EE909 pKa = 3.95QFLRR913 pKa = 11.84LAEE916 pKa = 4.2ALRR919 pKa = 11.84AAAEE923 pKa = 4.1KK924 pKa = 10.82DD925 pKa = 3.26PEE927 pKa = 4.23LRR929 pKa = 11.84RR930 pKa = 11.84LLAEE934 pKa = 4.46EE935 pKa = 4.7DD936 pKa = 3.65LVDD939 pKa = 4.61IIRR942 pKa = 11.84VEE944 pKa = 3.96NHH946 pKa = 6.55LKK948 pKa = 9.63QQAKK952 pKa = 9.65FAGEE956 pKa = 3.93PLRR959 pKa = 11.84KK960 pKa = 9.12IKK962 pKa = 10.58AGQGIDD968 pKa = 3.2AWSKK972 pKa = 10.27KK973 pKa = 9.79ANLVIGPFIRR983 pKa = 11.84AAQEE987 pKa = 3.57RR988 pKa = 11.84LLRR991 pKa = 11.84RR992 pKa = 11.84LKK994 pKa = 9.06PTVMMILHH1002 pKa = 6.04QSDD1005 pKa = 3.53ADD1007 pKa = 3.56VRR1009 pKa = 11.84DD1010 pKa = 4.44WIRR1013 pKa = 11.84SVHH1016 pKa = 5.67TGNTCVCNDD1025 pKa = 3.17FTEE1028 pKa = 4.7FDD1030 pKa = 3.65STQNDD1035 pKa = 3.38GTTRR1039 pKa = 11.84FEE1041 pKa = 4.44CLLLADD1047 pKa = 5.16LGVPKK1052 pKa = 10.32PIVNTYY1058 pKa = 9.44KK1059 pKa = 10.99ALRR1062 pKa = 11.84EE1063 pKa = 4.16SARR1066 pKa = 11.84VHH1068 pKa = 6.14ANGVVTDD1075 pKa = 4.04AAYY1078 pKa = 10.75ARR1080 pKa = 11.84MSGEE1084 pKa = 4.04ANTLFGNTVVTMAVNALLFPGAFDD1108 pKa = 3.35WAAFKK1113 pKa = 11.12GDD1115 pKa = 4.73DD1116 pKa = 5.22SIICNPRR1123 pKa = 11.84NRR1125 pKa = 11.84SDD1127 pKa = 3.66PDD1129 pKa = 3.83IISHH1133 pKa = 4.68QTGMQCKK1140 pKa = 9.44IEE1142 pKa = 4.04EE1143 pKa = 4.08QHH1145 pKa = 6.4VSEE1148 pKa = 5.08FVGLLISTDD1157 pKa = 3.33HH1158 pKa = 6.22VFPDD1162 pKa = 3.33LRR1164 pKa = 11.84RR1165 pKa = 11.84RR1166 pKa = 11.84VGKK1169 pKa = 8.71LTGRR1173 pKa = 11.84GYY1175 pKa = 9.98PGDD1178 pKa = 3.75PMARR1182 pKa = 11.84DD1183 pKa = 3.45QFSQSVRR1190 pKa = 11.84DD1191 pKa = 3.8TLALITPEE1199 pKa = 3.97STEE1202 pKa = 4.0ACILLNALAHH1212 pKa = 5.92HH1213 pKa = 6.66VSPAEE1218 pKa = 3.73AQVWYY1223 pKa = 9.82DD1224 pKa = 3.26VLIACVEE1231 pKa = 4.23GLGKK1235 pKa = 9.72PNRR1238 pKa = 11.84VEE1240 pKa = 4.7AIEE1243 pKa = 4.23ARR1245 pKa = 11.84LDD1247 pKa = 3.63EE1248 pKa = 5.03EE1249 pKa = 4.3II1250 pKa = 5.1

Molecular weight: 138.1 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A2U7MWY3|A0A2U7MWY3_9VIRU ORF1 OS=Bat Middle East Hepe-Astrovirus OX=2301732 PE=4 SV=1
MM1 pKa = 7.91PFRR4 pKa = 11.84MQGSRR9 pKa = 11.84SSSGRR14 pKa = 11.84ARR16 pKa = 11.84DD17 pKa = 3.46QRR19 pKa = 11.84SRR21 pKa = 11.84NLGQPRR27 pKa = 11.84LISARR32 pKa = 11.84SLARR36 pKa = 11.84TQMASPGSLTCSCTLLFCGTSMLRR60 pKa = 11.84TPPTPRR66 pKa = 11.84RR67 pKa = 11.84SEE69 pKa = 3.96RR70 pKa = 11.84RR71 pKa = 11.84STPCTVSQTWSLPCTAWWAPLASLVPSVSLRR102 pKa = 11.84CXLTLPRR109 pKa = 11.84AQPFPSTPSPRR120 pKa = 11.84ASTRR124 pKa = 11.84RR125 pKa = 11.84SASDD129 pKa = 3.11EE130 pKa = 4.13TDD132 pKa = 3.12GSNLAFHH139 pKa = 7.23PVRR142 pKa = 11.84ILGSSPTPRR151 pKa = 11.84RR152 pKa = 11.84TRR154 pKa = 11.84GPSRR158 pKa = 11.84SGKK161 pKa = 8.85RR162 pKa = 11.84WRR164 pKa = 11.84CSCLGQQTISTPTSPTLVHH183 pKa = 7.05FGDD186 pKa = 4.2SRR188 pKa = 11.84CGCVTSSPTTSRR200 pKa = 11.84TSSWRR205 pKa = 11.84LLRR208 pKa = 11.84QGRR211 pKa = 11.84RR212 pKa = 11.84LILPRR217 pKa = 11.84WPRR220 pKa = 11.84MRR222 pKa = 11.84PGTSQSRR229 pKa = 11.84QQSLSSAHH237 pKa = 6.62GSTHH241 pKa = 6.84PPQEE245 pKa = 4.44LPMSCSPSLTSQVGSLLKK263 pKa = 10.27FQSSAHH269 pKa = 6.64CSTQGWLSSSRR280 pKa = 11.84CSARR284 pKa = 11.84PGTGRR289 pKa = 11.84TSTSCAPVSTRR300 pKa = 11.84PRR302 pKa = 11.84LATPSQFQMPSTPQSPEE319 pKa = 3.05ISACNSSPPPTSVAPPKK336 pKa = 10.18PPLPRR341 pKa = 11.84RR342 pKa = 11.84PPAPSSCPPPPSAWLRR358 pKa = 11.84GQPATSRR365 pKa = 11.84SPSPRR370 pKa = 11.84GRR372 pKa = 11.84ARR374 pKa = 11.84WNEE377 pKa = 4.03LVRR380 pKa = 11.84QATTASLSLTPSPAAPPSYY399 pKa = 10.66HH400 pKa = 6.98PVTPPISPYY409 pKa = 10.64AVSGPCGATEE419 pKa = 4.1TRR421 pKa = 11.84PSSSGSYY428 pKa = 10.52VPMGRR433 pKa = 11.84PAEE436 pKa = 4.11RR437 pKa = 11.84TAPSSTSPPTRR448 pKa = 11.84RR449 pKa = 11.84GPWVSSVRR457 pKa = 11.84QQLLLTSPGYY467 pKa = 11.08CPMCHH472 pKa = 6.74RR473 pKa = 11.84ATIAWSLGGQRR484 pKa = 11.84SPPPRR489 pKa = 11.84VCPRR493 pKa = 11.84MYY495 pKa = 11.08DD496 pKa = 3.41PVTRR500 pKa = 11.84SYY502 pKa = 11.48QCISTTGSHH511 pKa = 5.96HH512 pKa = 5.15QTRR515 pKa = 11.84QNGRR519 pKa = 11.84PRR521 pKa = 11.84TAGCFADD528 pKa = 4.14LQRR531 pKa = 11.84SVSLL535 pKa = 3.84

Molecular weight: 57.53 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3

0

3

2403

535

1250

801.0

87.22

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

10.154 ± 1.29

2.289 ± 0.733

4.786 ± 1.241

4.12 ± 1.107

3.121 ± 0.539

7.158 ± 0.649

2.538 ± 0.374

3.204 ± 0.565

2.871 ± 0.766

8.656 ± 0.504

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.415 ± 0.15

2.58 ± 0.543

7.574 ± 2.338

4.702 ± 0.188

7.948 ± 1.484

7.824 ± 3.322

8.656 ± 1.196

6.617 ± 1.119

1.914 ± 0.053

1.789 ± 0.245
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski