Marmoricola ginsengisoli
Average proteome isoelectric point is 5.97
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 4237 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A3N0CN65|A0A3N0CN65_9ACTN Superoxide dismutase OS=Marmoricola ginsengisoli OX=661483 GN=EFK50_02655 PE=3 SV=1
MM1 pKa = 7.8 PGLPGLPGAPGTNGTNGNSLLNGAGAPDD29 pKa = 3.62 SALGSNGDD37 pKa = 3.78 YY38 pKa = 11.26 YY39 pKa = 11.6 LDD41 pKa = 3.62 TAASEE46 pKa = 4.65 LYY48 pKa = 10.43 GPKK51 pKa = 10.33 AGGAWPGSGVVLKK64 pKa = 10.92 GADD67 pKa = 3.71 GTNGTDD73 pKa = 3.83 GAPGAAGPQGPVGPPGADD91 pKa = 3.25 GTDD94 pKa = 3.6 GTDD97 pKa = 3.55 GADD100 pKa = 3.65 GADD103 pKa = 3.47 GAGTIFAASSGAAPAVATTVLGGLAGTGAALPLSGAFVEE142 pKa = 4.71 NGVTLTGGTIDD153 pKa = 3.68 STSLGVPQVFPRR165 pKa = 11.84 DD166 pKa = 3.7 GTVTAVTGRR175 pKa = 11.84 AVLSNALSLIGTTVTLQAQLYY196 pKa = 7.17 TSSSGGTTMTPVPGTQCILTPALTGVLTIGTVLDD230 pKa = 4.18 CSVTGLSIPVTQGTNAMMVVTATAAGISLVNTVTARR266 pKa = 11.84 LATSVAVSS274 pKa = 3.32
Molecular weight: 25.67 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.738
IPC2_protein 3.668
IPC_protein 3.643
Toseland 3.414
ProMoST 3.859
Dawson 3.681
Bjellqvist 3.834
Wikipedia 3.681
Rodwell 3.478
Grimsley 3.325
Solomon 3.656
Lehninger 3.605
Nozaki 3.808
DTASelect 4.113
Thurlkill 3.503
EMBOSS 3.681
Sillero 3.783
Patrickios 1.888
IPC_peptide 3.643
IPC2_peptide 3.745
IPC2.peptide.svr19 3.737
Protein with the highest isoelectric point:
>tr|A0A3N0CFN0|A0A3N0CFN0_9ACTN Glutaredoxin family protein OS=Marmoricola ginsengisoli OX=661483 GN=EFK50_10825 PE=4 SV=1
MM1 pKa = 7.4 GSVIKK6 pKa = 10.42 KK7 pKa = 8.47 RR8 pKa = 11.84 RR9 pKa = 11.84 KK10 pKa = 9.22 RR11 pKa = 11.84 MAKK14 pKa = 9.41 KK15 pKa = 9.99 KK16 pKa = 9.77 HH17 pKa = 5.81 RR18 pKa = 11.84 KK19 pKa = 8.51 LLKK22 pKa = 8.15 KK23 pKa = 9.12 TRR25 pKa = 11.84 VQRR28 pKa = 11.84 RR29 pKa = 11.84 RR30 pKa = 11.84 LGKK33 pKa = 10.04
Molecular weight: 4.1 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.493
IPC2_protein 11.14
IPC_protein 12.691
Toseland 12.866
ProMoST 13.349
Dawson 12.866
Bjellqvist 12.852
Wikipedia 13.334
Rodwell 12.735
Grimsley 12.91
Solomon 13.349
Lehninger 13.261
Nozaki 12.866
DTASelect 12.852
Thurlkill 12.866
EMBOSS 13.364
Sillero 12.866
Patrickios 12.457
IPC_peptide 13.349
IPC2_peptide 12.34
IPC2.peptide.svr19 9.071
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
4237
0
4237
1359760
29
4371
320.9
34.29
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
13.025 ± 0.053
0.735 ± 0.012
6.216 ± 0.03
5.568 ± 0.04
2.955 ± 0.022
9.243 ± 0.037
1.962 ± 0.016
4.045 ± 0.024
2.469 ± 0.027
10.088 ± 0.05
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
1.862 ± 0.018
2.141 ± 0.023
5.483 ± 0.028
2.739 ± 0.017
7.079 ± 0.045
5.509 ± 0.024
6.334 ± 0.053
9.071 ± 0.03
1.437 ± 0.016
2.039 ± 0.017
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here