Paenibacillus whitsoniae

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Firmicutes; Bacilli; Bacillales; Paenibacillaceae; Paenibacillus

Average proteome isoelectric point is 6.39

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 6143 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A430J4L0|A0A430J4L0_9BACL Histidine kinase OS=Paenibacillus whitsoniae OX=2496558 GN=EJQ19_30035 PE=4 SV=1
MM1 pKa = 6.91FTITSYY7 pKa = 9.85TVEE10 pKa = 3.91AVRR13 pKa = 11.84DD14 pKa = 3.82PFGILAGQRR23 pKa = 11.84YY24 pKa = 6.28EE25 pKa = 4.28FKK27 pKa = 10.69IEE29 pKa = 3.85IDD31 pKa = 3.68VEE33 pKa = 4.2EE34 pKa = 5.4DD35 pKa = 3.39DD36 pKa = 4.66EE37 pKa = 6.02LYY39 pKa = 10.42TEE41 pKa = 4.03SGLYY45 pKa = 9.93VRR47 pKa = 11.84VIYY50 pKa = 10.79NVTEE54 pKa = 3.99EE55 pKa = 4.11SAGILKK61 pKa = 10.6YY62 pKa = 10.39EE63 pKa = 4.42LYY65 pKa = 10.78EE66 pKa = 4.21KK67 pKa = 8.5TTEE70 pKa = 4.23LYY72 pKa = 11.05LDD74 pKa = 4.62FDD76 pKa = 4.77LEE78 pKa = 4.38DD79 pKa = 4.82DD80 pKa = 4.06EE81 pKa = 5.04VAEE84 pKa = 5.46LEE86 pKa = 4.52AFCKK90 pKa = 9.96AHH92 pKa = 5.87YY93 pKa = 10.62LEE95 pKa = 4.65GHH97 pKa = 5.82EE98 pKa = 4.83SEE100 pKa = 4.62

Molecular weight: 11.7 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A3S0A9D8|A0A3S0A9D8_9BACL UPF0178 protein EJQ19_20825 OS=Paenibacillus whitsoniae OX=2496558 GN=EJQ19_20825 PE=3 SV=1
MM1 pKa = 7.61GPTFNPNTRR10 pKa = 11.84KK11 pKa = 9.86RR12 pKa = 11.84KK13 pKa = 8.79KK14 pKa = 9.13NHH16 pKa = 5.06GFRR19 pKa = 11.84KK20 pKa = 9.98RR21 pKa = 11.84MSTKK25 pKa = 10.18NGRR28 pKa = 11.84KK29 pKa = 8.87VLAARR34 pKa = 11.84RR35 pKa = 11.84LKK37 pKa = 10.2GRR39 pKa = 11.84KK40 pKa = 8.79VLSAA44 pKa = 4.05

Molecular weight: 5.09 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

6143

0

6143

2161665

26

2882

351.9

39.06

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

8.84 ± 0.043

0.711 ± 0.009

5.086 ± 0.023

6.124 ± 0.036

4.141 ± 0.021

7.427 ± 0.031

2.108 ± 0.017

6.377 ± 0.031

5.17 ± 0.031

10.023 ± 0.045

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.791 ± 0.016

3.844 ± 0.033

4.17 ± 0.022

4.02 ± 0.019

4.801 ± 0.031

6.346 ± 0.031

5.831 ± 0.037

7.213 ± 0.024

1.374 ± 0.014

3.601 ± 0.021
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski