Pectobacterium phage MA11

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; unclassified Siphoviridae

Average proteome isoelectric point is 5.93

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 38 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A5Q2F9T2|A0A5Q2F9T2_9CAUD Portal protein OS=Pectobacterium phage MA11 OX=2662283 GN=MA11_gp14 PE=3 SV=1
MM1 pKa = 7.22ATINVLSEE9 pKa = 4.3ACQKK13 pKa = 10.54IYY15 pKa = 10.57GHH17 pKa = 6.33EE18 pKa = 4.2LPDD21 pKa = 4.92DD22 pKa = 3.51VRR24 pKa = 11.84ADD26 pKa = 3.93YY27 pKa = 11.09DD28 pKa = 3.88WISDD32 pKa = 4.46DD33 pKa = 4.3EE34 pKa = 4.59LSDD37 pKa = 4.03YY38 pKa = 11.29IFFTVEE44 pKa = 3.9GVTHH48 pKa = 6.47CVSDD52 pKa = 4.14FLRR55 pKa = 11.84CDD57 pKa = 3.78DD58 pKa = 3.7YY59 pKa = 11.42TAQGFHH65 pKa = 6.72AAYY68 pKa = 10.31SEE70 pKa = 4.29NAFSALLIGIIDD82 pKa = 4.26GDD84 pKa = 4.17DD85 pKa = 3.92DD86 pKa = 3.89NLKK89 pKa = 10.51VGRR92 pKa = 11.84VCC94 pKa = 5.43

Molecular weight: 10.5 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A5Q2F3L9|A0A5Q2F3L9_9CAUD DNA binding protein OS=Pectobacterium phage MA11 OX=2662283 GN=MA11_gp09 PE=4 SV=1
MM1 pKa = 7.56PRR3 pKa = 11.84HH4 pKa = 6.01HH5 pKa = 6.04WTARR9 pKa = 11.84EE10 pKa = 3.68PARR13 pKa = 11.84RR14 pKa = 11.84CTTHH18 pKa = 5.12ITSGYY23 pKa = 9.91RR24 pKa = 11.84YY25 pKa = 9.1FLTSEE30 pKa = 4.08TSLTGTTCRR39 pKa = 11.84KK40 pKa = 9.95QEE42 pKa = 4.1MNTMRR47 pKa = 11.84SPHH50 pKa = 5.74RR51 pKa = 11.84QPRR54 pKa = 11.84QYY56 pKa = 10.9SDD58 pKa = 3.51QVSCSACGKK67 pKa = 7.71TWDD70 pKa = 4.29VNDD73 pKa = 4.84PEE75 pKa = 4.68PPEE78 pKa = 4.26CVEE81 pKa = 4.34TLQQAIDD88 pKa = 3.31RR89 pKa = 11.84RR90 pKa = 11.84AAEE93 pKa = 4.18TSNRR97 pKa = 11.84LHH99 pKa = 6.9PVRR102 pKa = 11.84LALQAARR109 pKa = 11.84RR110 pKa = 11.84KK111 pKa = 9.87LNHH114 pKa = 6.65GKK116 pKa = 8.09TKK118 pKa = 9.37TT119 pKa = 3.32

Molecular weight: 13.73 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

38

0

38

14129

86

1491

371.8

41.1

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

10.588 ± 0.837

0.927 ± 0.175

6.858 ± 0.371

5.683 ± 0.394

3.758 ± 0.244

7.205 ± 0.27

1.543 ± 0.205

5.181 ± 0.15

5.145 ± 0.357

8.04 ± 0.279

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.668 ± 0.142

4.07 ± 0.15

4.445 ± 0.366

4.091 ± 0.419

5.775 ± 0.212

6.278 ± 0.228

6.186 ± 0.357

6.639 ± 0.272

1.769 ± 0.183

3.15 ± 0.252
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski