Amino acid dipepetide frequency for Enterobacteria phage S13 (Bacteriophage S13)

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
8.43AlaAla: 8.43 ± 1.534
2.81AlaCys: 2.81 ± 0.944
7.025AlaAsp: 7.025 ± 1.459
5.971AlaGlu: 5.971 ± 1.821
4.215AlaPhe: 4.215 ± 0.518
8.079AlaGly: 8.079 ± 3.1
1.054AlaHis: 1.054 ± 0.426
2.107AlaIle: 2.107 ± 0.915
6.322AlaLys: 6.322 ± 1.799
4.566AlaLeu: 4.566 ± 0.974
1.405AlaMet: 1.405 ± 0.445
3.161AlaAsn: 3.161 ± 0.661
2.107AlaPro: 2.107 ± 1.084
3.512AlaGln: 3.512 ± 0.882
2.107AlaArg: 2.107 ± 1.226
7.376AlaSer: 7.376 ± 1.29
5.971AlaThr: 5.971 ± 0.991
5.971AlaVal: 5.971 ± 1.076
0.351AlaTrp: 0.351 ± 0.347
1.405AlaTyr: 1.405 ± 0.646
0.0AlaXaa: 0.0 ± 0.0
Cys
0.0CysAla: 0.0 ± 0.0
0.351CysCys: 0.351 ± 0.322
0.702CysAsp: 0.702 ± 0.595
0.0CysGlu: 0.0 ± 0.0
0.351CysPhe: 0.351 ± 0.322
0.702CysGly: 0.702 ± 0.386
1.054CysHis: 1.054 ± 0.341
0.351CysIle: 0.351 ± 0.39
0.0CysLys: 0.0 ± 0.0
3.161CysLeu: 3.161 ± 0.918
0.0CysMet: 0.0 ± 0.0
0.0CysAsn: 0.0 ± 0.0
0.0CysPro: 0.0 ± 0.0
0.0CysGln: 0.0 ± 0.0
1.054CysArg: 1.054 ± 0.48
1.405CysSer: 1.405 ± 0.577
0.0CysThr: 0.0 ± 0.0
2.81CysVal: 2.81 ± 1.128
0.0CysTrp: 0.0 ± 0.0
1.756CysTyr: 1.756 ± 0.73
0.0CysXaa: 0.0 ± 0.0
Asp
7.376AspAla: 7.376 ± 0.907
1.405AspCys: 1.405 ± 0.465
2.107AspAsp: 2.107 ± 0.499
5.971AspGlu: 5.971 ± 2.07
1.756AspPhe: 1.756 ± 1.227
2.81AspGly: 2.81 ± 0.673
1.405AspHis: 1.405 ± 0.54
6.322AspIle: 6.322 ± 0.634
3.512AspLys: 3.512 ± 1.39
3.512AspLeu: 3.512 ± 0.781
1.756AspMet: 1.756 ± 0.73
3.512AspAsn: 3.512 ± 0.839
1.756AspPro: 1.756 ± 0.408
1.405AspGln: 1.405 ± 0.883
1.756AspArg: 1.756 ± 0.455
3.512AspSer: 3.512 ± 1.205
2.81AspThr: 2.81 ± 0.664
4.215AspVal: 4.215 ± 0.762
0.702AspTrp: 0.702 ± 0.493
2.81AspTyr: 2.81 ± 0.475
0.0AspXaa: 0.0 ± 0.0
Glu
5.269GluAla: 5.269 ± 1.357
2.459GluCys: 2.459 ± 1.087
0.702GluAsp: 0.702 ± 0.505
1.405GluGlu: 1.405 ± 0.58
2.459GluPhe: 2.459 ± 1.092
2.459GluGly: 2.459 ± 0.835
0.351GluHis: 0.351 ± 0.322
2.81GluIle: 2.81 ± 1.013
3.161GluLys: 3.161 ± 1.477
3.161GluLeu: 3.161 ± 1.07
2.81GluMet: 2.81 ± 0.962
2.81GluAsn: 2.81 ± 1.142
1.756GluPro: 1.756 ± 0.383
0.702GluGln: 0.702 ± 0.54
5.269GluArg: 5.269 ± 0.818
2.459GluSer: 2.459 ± 0.776
1.054GluThr: 1.054 ± 0.62
0.702GluVal: 0.702 ± 0.501
1.054GluTrp: 1.054 ± 0.426
1.756GluTyr: 1.756 ± 0.73
0.0GluXaa: 0.0 ± 0.0
Phe
1.054PheAla: 1.054 ± 0.65
1.756PheCys: 1.756 ± 0.73
4.215PheAsp: 4.215 ± 1.117
1.756PheGlu: 1.756 ± 0.432
1.405PhePhe: 1.405 ± 0.856
4.215PheGly: 4.215 ± 0.889
2.459PheHis: 2.459 ± 0.697
3.512PheIle: 3.512 ± 0.707
1.054PheLys: 1.054 ± 0.813
3.161PheLeu: 3.161 ± 1.005
4.215PheMet: 4.215 ± 0.725
1.405PheAsn: 1.405 ± 0.358
1.405PhePro: 1.405 ± 0.753
1.756PheGln: 1.756 ± 0.89
4.215PheArg: 4.215 ± 0.99
1.756PheSer: 1.756 ± 0.495
4.215PheThr: 4.215 ± 0.672
2.107PheVal: 2.107 ± 0.782
0.702PheTrp: 0.702 ± 0.441
2.81PheTyr: 2.81 ± 0.682
0.0PheXaa: 0.0 ± 0.0
Gly
5.62GlyAla: 5.62 ± 1.782
0.0GlyCys: 0.0 ± 0.0
3.512GlyAsp: 3.512 ± 1.021
1.405GlyGlu: 1.405 ± 0.358
5.269GlyPhe: 5.269 ± 0.847
5.269GlyGly: 5.269 ± 1.919
0.702GlyHis: 0.702 ± 0.386
4.566GlyIle: 4.566 ± 1.95
5.269GlyLys: 5.269 ± 1.276
3.864GlyLeu: 3.864 ± 0.873
1.405GlyMet: 1.405 ± 0.445
1.405GlyAsn: 1.405 ± 0.911
0.0GlyPro: 0.0 ± 0.0
2.107GlyGln: 2.107 ± 1.228
4.917GlyArg: 4.917 ± 1.001
2.107GlySer: 2.107 ± 0.978
3.864GlyThr: 3.864 ± 0.976
3.864GlyVal: 3.864 ± 0.813
2.107GlyTrp: 2.107 ± 0.852
3.864GlyTyr: 3.864 ± 0.609
0.0GlyXaa: 0.0 ± 0.0
His
4.215HisAla: 4.215 ± 1.373
0.702HisCys: 0.702 ± 0.509
1.405HisAsp: 1.405 ± 0.805
0.0HisGlu: 0.0 ± 0.0
2.459HisPhe: 2.459 ± 0.861
1.405HisGly: 1.405 ± 0.66
0.351HisHis: 0.351 ± 0.322
0.351HisIle: 0.351 ± 0.347
1.405HisLys: 1.405 ± 0.772
1.405HisLeu: 1.405 ± 1.29
0.0HisMet: 0.0 ± 0.0
0.702HisAsn: 0.702 ± 0.515
1.054HisPro: 1.054 ± 0.526
0.702HisGln: 0.702 ± 0.486
0.351HisArg: 0.351 ± 0.322
0.351HisSer: 0.351 ± 0.322
0.351HisThr: 0.351 ± 0.322
0.351HisVal: 0.351 ± 0.322
0.0HisTrp: 0.0 ± 0.0
0.0HisTyr: 0.0 ± 0.0
0.0HisXaa: 0.0 ± 0.0
Ile
5.971IleAla: 5.971 ± 1.262
0.351IleCys: 0.351 ± 0.39
2.459IleAsp: 2.459 ± 0.832
2.107IleGlu: 2.107 ± 0.945
0.702IlePhe: 0.702 ± 0.441
3.512IleGly: 3.512 ± 0.723
0.0IleHis: 0.0 ± 0.0
1.054IleIle: 1.054 ± 0.778
5.62IleLys: 5.62 ± 0.942
3.161IleLeu: 3.161 ± 1.34
2.107IleMet: 2.107 ± 0.996
1.405IleAsn: 1.405 ± 0.483
0.702IlePro: 0.702 ± 0.57
5.269IleGln: 5.269 ± 0.837
2.459IleArg: 2.459 ± 0.637
3.512IleSer: 3.512 ± 0.619
1.405IleThr: 1.405 ± 0.358
1.405IleVal: 1.405 ± 0.445
0.351IleTrp: 0.351 ± 0.322
0.702IleTyr: 0.702 ± 0.438
0.0IleXaa: 0.0 ± 0.0
Lys
6.674LysAla: 6.674 ± 1.125
0.351LysCys: 0.351 ± 0.39
4.215LysAsp: 4.215 ± 1.176
3.864LysGlu: 3.864 ± 1.591
2.81LysPhe: 2.81 ± 0.887
4.917LysGly: 4.917 ± 1.535
0.702LysHis: 0.702 ± 0.645
1.405LysIle: 1.405 ± 1.036
5.269LysLys: 5.269 ± 1.231
8.43LysLeu: 8.43 ± 2.189
3.864LysMet: 3.864 ± 1.128
2.81LysAsn: 2.81 ± 0.819
1.054LysPro: 1.054 ± 0.528
2.459LysGln: 2.459 ± 0.738
2.459LysArg: 2.459 ± 1.075
5.269LysSer: 5.269 ± 1.348
3.161LysThr: 3.161 ± 0.994
2.107LysVal: 2.107 ± 0.488
2.107LysTrp: 2.107 ± 0.74
1.054LysTyr: 1.054 ± 0.426
0.0LysXaa: 0.0 ± 0.0
Leu
9.835LeuAla: 9.835 ± 1.115
0.351LeuCys: 0.351 ± 0.455
3.512LeuAsp: 3.512 ± 0.911
4.215LeuGlu: 4.215 ± 1.175
2.107LeuPhe: 2.107 ± 0.379
5.62LeuGly: 5.62 ± 1.017
1.756LeuHis: 1.756 ± 0.408
3.512LeuIle: 3.512 ± 1.118
7.025LeuLys: 7.025 ± 1.881
10.537LeuLeu: 10.537 ± 4.192
2.107LeuMet: 2.107 ± 0.777
4.917LeuAsn: 4.917 ± 1.403
5.62LeuPro: 5.62 ± 1.313
4.215LeuGln: 4.215 ± 0.821
6.322LeuArg: 6.322 ± 1.178
8.43LeuSer: 8.43 ± 1.294
7.025LeuThr: 7.025 ± 1.204
3.161LeuVal: 3.161 ± 1.595
1.756LeuTrp: 1.756 ± 0.7
0.702LeuTyr: 0.702 ± 0.509
0.0LeuXaa: 0.0 ± 0.0
Met
1.756MetAla: 1.756 ± 0.551
0.0MetCys: 0.0 ± 0.0
3.161MetAsp: 3.161 ± 0.868
2.107MetGlu: 2.107 ± 0.566
2.459MetPhe: 2.459 ± 0.507
0.702MetGly: 0.702 ± 0.441
0.0MetHis: 0.0 ± 0.0
1.054MetIle: 1.054 ± 0.426
2.81MetLys: 2.81 ± 0.715
1.756MetLeu: 1.756 ± 0.706
0.0MetMet: 0.0 ± 0.35
1.054MetAsn: 1.054 ± 0.426
2.107MetPro: 2.107 ± 0.578
1.756MetGln: 1.756 ± 0.596
5.269MetArg: 5.269 ± 1.397
2.81MetSer: 2.81 ± 0.537
3.161MetThr: 3.161 ± 0.604
2.459MetVal: 2.459 ± 0.745
0.0MetTrp: 0.0 ± 0.0
0.702MetTyr: 0.702 ± 0.386
0.0MetXaa: 0.0 ± 0.0
Asn
4.917AsnAla: 4.917 ± 0.864
0.351AsnCys: 0.351 ± 0.442
2.459AsnAsp: 2.459 ± 0.851
2.81AsnGlu: 2.81 ± 0.81
3.864AsnPhe: 3.864 ± 0.79
2.81AsnGly: 2.81 ± 0.884
0.0AsnHis: 0.0 ± 0.0
1.405AsnIle: 1.405 ± 1.011
1.756AsnLys: 1.756 ± 0.455
5.269AsnLeu: 5.269 ± 0.929
0.702AsnMet: 0.702 ± 0.607
3.161AsnAsn: 3.161 ± 0.561
2.107AsnPro: 2.107 ± 0.531
3.512AsnGln: 3.512 ± 1.303
2.459AsnArg: 2.459 ± 0.637
3.161AsnSer: 3.161 ± 0.564
3.864AsnThr: 3.864 ± 0.85
3.161AsnVal: 3.161 ± 0.791
0.0AsnTrp: 0.0 ± 0.0
2.459AsnTyr: 2.459 ± 0.662
0.0AsnXaa: 0.0 ± 0.0
Pro
2.107ProAla: 2.107 ± 0.617
0.351ProCys: 0.351 ± 0.442
1.054ProAsp: 1.054 ± 0.696
2.81ProGlu: 2.81 ± 0.475
1.405ProPhe: 1.405 ± 0.358
0.702ProGly: 0.702 ± 0.693
0.702ProHis: 0.702 ± 0.645
1.405ProIle: 1.405 ± 0.555
2.81ProLys: 2.81 ± 0.764
4.917ProLeu: 4.917 ± 1.403
0.702ProMet: 0.702 ± 0.386
3.161ProAsn: 3.161 ± 0.722
2.459ProPro: 2.459 ± 1.126
1.405ProGln: 1.405 ± 0.687
1.405ProArg: 1.405 ± 0.834
3.864ProSer: 3.864 ± 0.9
2.107ProThr: 2.107 ± 1.274
4.566ProVal: 4.566 ± 1.191
0.351ProTrp: 0.351 ± 0.322
1.054ProTyr: 1.054 ± 0.426
0.0ProXaa: 0.0 ± 0.0
Gln
3.161GlnAla: 3.161 ± 0.843
0.702GlnCys: 0.702 ± 0.386
1.054GlnAsp: 1.054 ± 0.426
2.107GlnGlu: 2.107 ± 1.166
1.405GlnPhe: 1.405 ± 0.669
1.756GlnGly: 1.756 ± 0.596
0.0GlnHis: 0.0 ± 0.0
2.107GlnIle: 2.107 ± 0.396
3.864GlnLys: 3.864 ± 1.687
4.917GlnLeu: 4.917 ± 0.963
1.405GlnMet: 1.405 ± 0.496
4.215GlnAsn: 4.215 ± 1.471
2.81GlnPro: 2.81 ± 0.874
2.107GlnGln: 2.107 ± 1.394
1.756GlnArg: 1.756 ± 0.339
2.81GlnSer: 2.81 ± 0.482
3.161GlnThr: 3.161 ± 1.454
2.107GlnVal: 2.107 ± 1.047
0.702GlnTrp: 0.702 ± 0.645
1.756GlnTyr: 1.756 ± 0.339
0.0GlnXaa: 0.0 ± 0.0
Arg
2.459ArgAla: 2.459 ± 1.134
0.702ArgCys: 0.702 ± 0.505
6.322ArgAsp: 6.322 ± 1.062
1.405ArgGlu: 1.405 ± 0.358
2.81ArgPhe: 2.81 ± 1.463
2.459ArgGly: 2.459 ± 0.716
1.756ArgHis: 1.756 ± 1.095
3.864ArgIle: 3.864 ± 1.426
4.215ArgLys: 4.215 ± 0.968
7.727ArgLeu: 7.727 ± 2.076
3.512ArgMet: 3.512 ± 0.89
2.459ArgAsn: 2.459 ± 0.717
2.81ArgPro: 2.81 ± 1.418
3.161ArgGln: 3.161 ± 0.841
5.971ArgArg: 5.971 ± 1.325
4.215ArgSer: 4.215 ± 1.133
1.756ArgThr: 1.756 ± 0.408
2.459ArgVal: 2.459 ± 0.507
0.702ArgTrp: 0.702 ± 0.57
2.81ArgTyr: 2.81 ± 0.803
0.0ArgXaa: 0.0 ± 0.0
Ser
3.161SerAla: 3.161 ± 2.451
0.0SerCys: 0.0 ± 0.0
5.269SerAsp: 5.269 ± 1.291
1.405SerGlu: 1.405 ± 0.488
2.81SerPhe: 2.81 ± 0.475
5.269SerGly: 5.269 ± 1.076
2.107SerHis: 2.107 ± 0.424
2.459SerIle: 2.459 ± 0.507
3.864SerLys: 3.864 ± 0.693
5.971SerLeu: 5.971 ± 1.529
4.215SerMet: 4.215 ± 1.074
3.864SerAsn: 3.864 ± 1.03
1.756SerPro: 1.756 ± 0.626
1.756SerGln: 1.756 ± 0.557
7.025SerArg: 7.025 ± 0.899
4.566SerSer: 4.566 ± 2.234
3.864SerThr: 3.864 ± 0.853
5.269SerVal: 5.269 ± 1.538
0.351SerTrp: 0.351 ± 0.442
3.512SerTyr: 3.512 ± 0.755
0.0SerXaa: 0.0 ± 0.0
Thr
6.322ThrAla: 6.322 ± 0.837
0.702ThrCys: 0.702 ± 0.532
3.161ThrAsp: 3.161 ± 1.544
2.459ThrGlu: 2.459 ± 1.259
2.459ThrPhe: 2.459 ± 1.327
0.351ThrGly: 0.351 ± 0.322
0.0ThrHis: 0.0 ± 0.0
3.161ThrIle: 3.161 ± 0.608
4.215ThrLys: 4.215 ± 0.645
8.43ThrLeu: 8.43 ± 1.947
2.107ThrMet: 2.107 ± 0.585
3.512ThrAsn: 3.512 ± 0.699
2.81ThrPro: 2.81 ± 1.15
4.215ThrGln: 4.215 ± 1.177
1.054ThrArg: 1.054 ± 0.726
5.269ThrSer: 5.269 ± 1.773
4.215ThrThr: 4.215 ± 1.299
3.864ThrVal: 3.864 ± 0.805
1.405ThrTrp: 1.405 ± 0.445
1.054ThrTyr: 1.054 ± 0.696
0.0ThrXaa: 0.0 ± 0.0
Val
3.512ValAla: 3.512 ± 0.839
0.0ValCys: 0.0 ± 0.0
4.215ValAsp: 4.215 ± 0.889
1.756ValGlu: 1.756 ± 1.227
1.405ValPhe: 1.405 ± 0.358
3.512ValGly: 3.512 ± 0.921
2.81ValHis: 2.81 ± 0.831
1.756ValIle: 1.756 ± 1.067
2.107ValLys: 2.107 ± 1.051
5.269ValLeu: 5.269 ± 0.885
1.054ValMet: 1.054 ± 0.544
3.161ValAsn: 3.161 ± 0.855
2.81ValPro: 2.81 ± 0.777
2.107ValGln: 2.107 ± 1.185
5.971ValArg: 5.971 ± 1.547
4.215ValSer: 4.215 ± 0.941
5.269ValThr: 5.269 ± 1.015
1.054ValVal: 1.054 ± 0.779
0.702ValTrp: 0.702 ± 0.542
3.864ValTyr: 3.864 ± 1.186
0.0ValXaa: 0.0 ± 0.0
Trp
0.351TrpAla: 0.351 ± 0.322
0.0TrpCys: 0.0 ± 0.0
0.351TrpAsp: 0.351 ± 0.442
0.351TrpGlu: 0.351 ± 0.347
2.107TrpPhe: 2.107 ± 0.74
0.0TrpGly: 0.0 ± 0.0
0.0TrpHis: 0.0 ± 0.0
0.702TrpIle: 0.702 ± 0.505
0.702TrpLys: 0.702 ± 0.534
1.405TrpLeu: 1.405 ± 0.502
0.351TrpMet: 0.351 ± 0.322
1.756TrpAsn: 1.756 ± 0.339
2.107TrpPro: 2.107 ± 0.852
0.0TrpGln: 0.0 ± 0.0
0.351TrpArg: 0.351 ± 0.328
0.351TrpSer: 0.351 ± 0.39
1.756TrpThr: 1.756 ± 0.538
0.0TrpVal: 0.0 ± 0.0
0.0TrpTrp: 0.0 ± 0.0
0.702TrpTyr: 0.702 ± 0.65
0.0TrpXaa: 0.0 ± 0.0
Tyr
1.756TyrAla: 1.756 ± 0.873
0.351TyrCys: 0.351 ± 0.328
3.512TyrAsp: 3.512 ± 0.803
0.702TyrGlu: 0.702 ± 0.649
4.917TyrPhe: 4.917 ± 0.551
4.566TyrGly: 4.566 ± 0.702
0.351TyrHis: 0.351 ± 0.322
0.351TyrIle: 0.351 ± 0.322
0.351TyrLys: 0.351 ± 0.322
2.107TyrLeu: 2.107 ± 0.49
1.054TyrMet: 1.054 ± 0.426
1.756TyrAsn: 1.756 ± 0.653
2.107TyrPro: 2.107 ± 0.824
1.756TyrGln: 1.756 ± 0.51
1.405TyrArg: 1.405 ± 0.992
1.054TyrSer: 1.054 ± 0.426
1.756TyrThr: 1.756 ± 0.408
4.917TyrVal: 4.917 ± 0.956
0.0TyrTrp: 0.0 ± 0.0
1.405TyrTyr: 1.405 ± 0.581
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 12 proteins (2848 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski