Flavobacterium beibuense F44-8

Taxonomy: cellular organisms; Bacteria; FCB group; Bacteroidetes/Chlorobi group; Bacteroidetes; Flavobacteriia; Flavobacteriales; Flavobacteriaceae; Flavobacterium; Flavobacterium beibuense

Average proteome isoelectric point is 6.27

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3263 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A0A2LWF6|A0A0A2LWF6_9FLAO Peptidase M22 OS=Flavobacterium beibuense F44-8 OX=1406840 GN=Q763_10825 PE=4 SV=1
MM1 pKa = 7.46KK2 pKa = 10.52KK3 pKa = 10.22KK4 pKa = 10.46LLYY7 pKa = 10.77LLLLFSGIASAQIVNIPDD25 pKa = 3.55ANFKK29 pKa = 11.05NKK31 pKa = 9.8LLSANTTNNIAKK43 pKa = 9.95DD44 pKa = 3.83EE45 pKa = 4.38NGVAIKK51 pKa = 9.98IDD53 pKa = 3.45QNNDD57 pKa = 3.02YY58 pKa = 10.38EE59 pKa = 4.44IQEE62 pKa = 4.28SEE64 pKa = 4.36ALLVWEE70 pKa = 5.33LSLFSAGMADD80 pKa = 3.42ATGLEE85 pKa = 4.68YY86 pKa = 9.32FTNLRR91 pKa = 11.84SLEE94 pKa = 4.12CANNSDD100 pKa = 5.25LVYY103 pKa = 10.86LDD105 pKa = 4.69LSSLINLEE113 pKa = 5.09DD114 pKa = 4.31FNCDD118 pKa = 2.73GGTGLGFLSFSGLFNLQTVYY138 pKa = 10.67ISDD141 pKa = 4.02TNLEE145 pKa = 4.46TIDD148 pKa = 3.63LTGVTGVQEE157 pKa = 4.48FEE159 pKa = 4.51LEE161 pKa = 4.33GVPVTSLDD169 pKa = 3.51FSDD172 pKa = 4.68MINLYY177 pKa = 10.63SLALSGTEE185 pKa = 5.06LVNLDD190 pKa = 4.54LGDD193 pKa = 4.54APLVTYY199 pKa = 10.73INLSYY204 pKa = 11.43NDD206 pKa = 3.62VLEE209 pKa = 4.75TISIKK214 pKa = 10.7NGAIIPSPSNIHH226 pKa = 6.52INNNNPQLNFICIDD240 pKa = 3.73EE241 pKa = 5.21DD242 pKa = 3.6EE243 pKa = 4.86LSQIEE248 pKa = 4.36LVDD251 pKa = 4.7GIEE254 pKa = 4.48NIVVSTYY261 pKa = 9.99CTFTPGGDD269 pKa = 3.52YY270 pKa = 10.19NTITGTAVFDD280 pKa = 4.67ADD282 pKa = 4.23SDD284 pKa = 4.3GCGPEE289 pKa = 4.27DD290 pKa = 3.84FPYY293 pKa = 10.71PFVKK297 pKa = 10.7LSINDD302 pKa = 3.93GVEE305 pKa = 3.57GGYY308 pKa = 8.37TFTGVNGEE316 pKa = 4.19YY317 pKa = 10.57SFYY320 pKa = 10.27TQDD323 pKa = 3.02GNFTVVPEE331 pKa = 5.02FEE333 pKa = 5.75DD334 pKa = 2.96NWFTVTPAYY343 pKa = 11.24VNFPVVDD350 pKa = 3.72NSVSVQNFCIEE361 pKa = 4.26PNGVHH366 pKa = 6.57NDD368 pKa = 3.43VEE370 pKa = 4.83VVMVPITPARR380 pKa = 11.84PGFDD384 pKa = 3.87AIYY387 pKa = 10.33KK388 pKa = 9.78IVYY391 pKa = 8.82KK392 pKa = 10.95NKK394 pKa = 9.18GNQVVSGTVGCEE406 pKa = 3.38WDD408 pKa = 4.13YY409 pKa = 11.12EE410 pKa = 4.53TLTPLVLNPMADD422 pKa = 4.07DD423 pKa = 4.08IAPGVYY429 pKa = 8.98GWNYY433 pKa = 10.15TNLQPFEE440 pKa = 4.06NRR442 pKa = 11.84EE443 pKa = 3.76ILMTLHH449 pKa = 6.4VNSPTDD455 pKa = 3.57TPAVNIDD462 pKa = 3.56DD463 pKa = 4.3VLPFTANIDD472 pKa = 3.43GGTDD476 pKa = 3.26EE477 pKa = 5.41NPNNNQYY484 pKa = 10.4IFNQTVVGSFDD495 pKa = 4.2PNNIICIEE503 pKa = 4.12GEE505 pKa = 4.38TLPSNAIGDD514 pKa = 3.91YY515 pKa = 10.52LHH517 pKa = 6.64YY518 pKa = 10.77VVNFEE523 pKa = 4.34NTGTAPASFVVVKK536 pKa = 9.67HH537 pKa = 6.13VINEE541 pKa = 3.77ADD543 pKa = 3.32FDD545 pKa = 4.64INSIQILNSSHH556 pKa = 5.18QVEE559 pKa = 5.4ARR561 pKa = 11.84IQDD564 pKa = 3.6NVIEE568 pKa = 5.06FIFDD572 pKa = 4.07NINLNAADD580 pKa = 4.26HH581 pKa = 6.3GNILFKK587 pKa = 10.98LKK589 pKa = 10.5SKK591 pKa = 10.93QDD593 pKa = 3.59LQEE596 pKa = 4.24GDD598 pKa = 3.35NVMNEE603 pKa = 3.33AGIYY607 pKa = 9.84FDD609 pKa = 4.53YY610 pKa = 10.86NLPVATNQAVTEE622 pKa = 4.42FEE624 pKa = 5.69DD625 pKa = 5.13IMGLDD630 pKa = 5.11DD631 pKa = 5.4FIKK634 pKa = 10.86DD635 pKa = 3.57NSIKK639 pKa = 10.59VYY641 pKa = 10.17PNPAKK646 pKa = 10.54DD647 pKa = 3.63VLSVKK652 pKa = 10.59GDD654 pKa = 3.35VNLRR658 pKa = 11.84SVSLYY663 pKa = 10.46DD664 pKa = 3.16IQGRR668 pKa = 11.84LLQTVMPNDD677 pKa = 3.51VQVTIDD683 pKa = 2.72IAMRR687 pKa = 11.84AKK689 pKa = 10.53GIYY692 pKa = 8.75FLKK695 pKa = 9.27VTSDD699 pKa = 3.15KK700 pKa = 10.9GVKK703 pKa = 8.68VEE705 pKa = 4.6KK706 pKa = 10.46VIKK709 pKa = 9.81EE710 pKa = 3.93

Molecular weight: 78.17 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A0A2LJ55|A0A0A2LJ55_9FLAO Transporter OS=Flavobacterium beibuense F44-8 OX=1406840 GN=Q763_11335 PE=3 SV=1
MM1 pKa = 7.5SFVTNIRR8 pKa = 11.84HH9 pKa = 4.78FFEE12 pKa = 5.25RR13 pKa = 11.84NGFHH17 pKa = 6.58VSARR21 pKa = 11.84LSDD24 pKa = 3.7RR25 pKa = 11.84LGMRR29 pKa = 11.84VTNVRR34 pKa = 11.84LFFIYY39 pKa = 10.23ISFFTVGLWFGVYY52 pKa = 8.36LTLAFWLRR60 pKa = 11.84LKK62 pKa = 10.75DD63 pKa = 3.42AVYY66 pKa = 9.69TKK68 pKa = 10.33RR69 pKa = 11.84SSVFDD74 pKa = 3.68LL75 pKa = 4.35

Molecular weight: 8.98 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3263

0

3263

1084350

46

3813

332.3

37.45

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.666 ± 0.049

0.811 ± 0.018

5.724 ± 0.034

6.748 ± 0.043

5.03 ± 0.039

6.491 ± 0.046

1.706 ± 0.021

7.408 ± 0.041

7.374 ± 0.079

8.915 ± 0.057

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.337 ± 0.021

6.161 ± 0.045

3.504 ± 0.03

3.418 ± 0.024

3.361 ± 0.036

6.138 ± 0.037

6.198 ± 0.07

6.544 ± 0.042

1.036 ± 0.016

4.432 ± 0.028
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski