Mycoplasma flocculare ATCC 27399

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Tenericutes; Mollicutes; Mycoplasmatales; Mycoplasmataceae; Mycoplasma; Mycoplasma flocculare

Average proteome isoelectric point is 8.13

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 553 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A0A8E6Z4|A0A0A8E6Z4_MYCFL Oligopeptide ABC transporter permease protein OS=Mycoplasma flocculare ATCC 27399 OX=743971 GN=oppC PE=3 SV=1
MM1 pKa = 8.08PYY3 pKa = 10.45LDD5 pKa = 4.09KK6 pKa = 10.88QRR8 pKa = 11.84KK9 pKa = 9.25DD10 pKa = 3.31GLLQSDD16 pKa = 3.97AFYY19 pKa = 11.55NEE21 pKa = 4.13LFLGLFGFGFWYY33 pKa = 10.23LGRR36 pKa = 11.84VSLAIIRR43 pKa = 11.84FTFTIVGLLMIFIPQMMYY61 pKa = 10.63LGSTAQDD68 pKa = 3.53EE69 pKa = 4.79IKK71 pKa = 10.84NDD73 pKa = 3.49VYY75 pKa = 11.08ILTIVGGVILAVSLLWNIITTIMIFTADD103 pKa = 3.43MRR105 pKa = 11.84DD106 pKa = 3.57DD107 pKa = 3.76RR108 pKa = 11.84GGKK111 pKa = 8.2ILHH114 pKa = 6.86WGTEE118 pKa = 3.97IEE120 pKa = 4.76DD121 pKa = 3.86NPDD124 pKa = 3.23LEE126 pKa = 4.73DD127 pKa = 4.2AFDD130 pKa = 3.83IPVEE134 pKa = 4.31EE135 pKa = 4.67EE136 pKa = 3.65EE137 pKa = 4.49STPVLAMEE145 pKa = 4.31TEE147 pKa = 4.47SVIEE151 pKa = 3.95EE152 pKa = 4.38TEE154 pKa = 4.0ALDD157 pKa = 3.82LQVEE161 pKa = 4.44SLEE164 pKa = 4.43PEE166 pKa = 4.13SLEE169 pKa = 3.98PEE171 pKa = 4.45PVTEE175 pKa = 3.93PVYY178 pKa = 10.59FEE180 pKa = 3.68PVKK183 pKa = 10.49PVFEE187 pKa = 4.7PEE189 pKa = 4.1FVTDD193 pKa = 3.79PVVAEE198 pKa = 4.49PVFGTQPVKK207 pKa = 10.41PVAEE211 pKa = 4.32PPVAEE216 pKa = 4.11PVVKK220 pKa = 10.11PVFEE224 pKa = 4.35PVAEE228 pKa = 4.05PVKK231 pKa = 10.31PVAEE235 pKa = 4.01PVVKK239 pKa = 10.01PVFEE243 pKa = 4.25VEE245 pKa = 4.2EE246 pKa = 4.35NFDD249 pKa = 3.26IARR252 pKa = 11.84QIEE255 pKa = 4.72MINTEE260 pKa = 4.05QVQISQLQTVQKK272 pKa = 11.15AEE274 pKa = 3.95IPVDD278 pKa = 4.43FSALKK283 pKa = 9.98HH284 pKa = 5.22IHH286 pKa = 6.03HH287 pKa = 6.52TTFTIQEE294 pKa = 4.1IEE296 pKa = 4.06SGIVDD301 pKa = 3.32EE302 pKa = 4.84HH303 pKa = 7.86FEE305 pKa = 4.43IRR307 pKa = 11.84GQHH310 pKa = 7.09DD311 pKa = 3.81ISCHH315 pKa = 4.88KK316 pKa = 10.67

Molecular weight: 35.67 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A0A8E7D3|A0A0A8E7D3_MYCFL Ribosomal RNA small subunit methyltransferase G OS=Mycoplasma flocculare ATCC 27399 OX=743971 GN=gidB PE=3 SV=1
MM1 pKa = 7.37ARR3 pKa = 11.84KK4 pKa = 9.96DD5 pKa = 3.99PISQRR10 pKa = 11.84GPLSGNNRR18 pKa = 11.84SHH20 pKa = 7.23ALNATKK26 pKa = 10.38RR27 pKa = 11.84KK28 pKa = 9.59FNLNLQQVTIKK39 pKa = 9.49TASRR43 pKa = 11.84QKK45 pKa = 10.3IRR47 pKa = 11.84LKK49 pKa = 11.04VSTKK53 pKa = 7.89TKK55 pKa = 8.64KK56 pKa = 8.17TLRR59 pKa = 11.84KK60 pKa = 8.66WGQII64 pKa = 3.41

Molecular weight: 7.34 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

553

0

553

221749

37

3784

401.0

46.03

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

5.337 ± 0.088

0.422 ± 0.028

4.663 ± 0.064

6.438 ± 0.091

6.887 ± 0.102

4.575 ± 0.097

1.273 ± 0.044

9.908 ± 0.146

10.755 ± 0.09

9.817 ± 0.075

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.223 ± 0.042

7.69 ± 0.118

2.959 ± 0.049

3.931 ± 0.073

2.905 ± 0.06

7.108 ± 0.101

4.668 ± 0.076

4.86 ± 0.064

0.952 ± 0.031

3.628 ± 0.065
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski